chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.
Abstract
Chlorophylls (Chls) play pivotal roles in energy absorption and transduction and also in charge separation in reaction centers in all photosynthetic organisms. In Chl biosynthesis steps, only a step for the enzymatic reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) is mediated by both nuclear- and chloroplast-encoded genes in land plants. Many plants encode the genes for light-dependent Pchlide reductase (LPOR) and light-independent Pchlide reductase (DPOR) in the nucleus and chloroplast genome, respectively. During the diversification of land plants, the reduction step of Pchlide to Chlide has become solely dependent on LPOR, and the genes for DPOR have been lost from chloroplast genome. It remains unclear why DPOR persists in some land plants, how they were eliminated from chloroplast genomes during the diversification of land plants, and under what environmental conditions DPOR was required. We demonstrate that DPOR is functional in liverwort (Marchantia polymorpha L.) and plays an important role in Chl biosynthesis. Having established a plastid transformation system in liverwort, we disrupted chlB, which encodes a subunit of DPOR in the M. polymorpha chloroplast genome. Morphological and Chl content analysis of a chlB mutant grown under different photoperiods revealed that DPOR is particularly required for Chl biosynthesis under short-day conditions.more »
- Authors:
-
- Kyoto Univ. (Japan). Graduate School of Science. Dept. of Botany
- Hokkaido Univ., Sapporo (Japan). Inst. of Low Temperature Science
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); Japan Society for the Promotion of Science (JSPS)
- OSTI Identifier:
- 1625333
- Grant/Contract Number:
- AC02-05CH11231; 25650126
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Genome Biology and Evolution
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 3; Journal ID: ISSN 1759-6653
- Publisher:
- Society for Molecular Biology and Evolution
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Evolutionary Biology; Genetics & Heredity; plastid transformation; chloroplast genome evolution; bryophyte; chlorophyll metabolism
Citation Formats
Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, and Nishimura, Yoshiki. chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.. United States: N. p., 2014.
Web. doi:10.1093/gbe/evu045.
Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, & Nishimura, Yoshiki. chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.. United States. https://doi.org/10.1093/gbe/evu045
Ueda, Minoru, Tanaka, Ayumi, Sugimoto, Kazuhiko, Shikanai, Toshiharu, and Nishimura, Yoshiki. Thu .
"chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.". United States. https://doi.org/10.1093/gbe/evu045. https://www.osti.gov/servlets/purl/1625333.
@article{osti_1625333,
title = {chlB Requirement for Chlorophyll Biosynthesis under Short Photoperiod in Marchantia polymorpha L.},
author = {Ueda, Minoru and Tanaka, Ayumi and Sugimoto, Kazuhiko and Shikanai, Toshiharu and Nishimura, Yoshiki},
abstractNote = {Chlorophylls (Chls) play pivotal roles in energy absorption and transduction and also in charge separation in reaction centers in all photosynthetic organisms. In Chl biosynthesis steps, only a step for the enzymatic reduction of protochlorophyllide (Pchlide) to chlorophyllide (Chlide) is mediated by both nuclear- and chloroplast-encoded genes in land plants. Many plants encode the genes for light-dependent Pchlide reductase (LPOR) and light-independent Pchlide reductase (DPOR) in the nucleus and chloroplast genome, respectively. During the diversification of land plants, the reduction step of Pchlide to Chlide has become solely dependent on LPOR, and the genes for DPOR have been lost from chloroplast genome. It remains unclear why DPOR persists in some land plants, how they were eliminated from chloroplast genomes during the diversification of land plants, and under what environmental conditions DPOR was required. We demonstrate that DPOR is functional in liverwort (Marchantia polymorpha L.) and plays an important role in Chl biosynthesis. Having established a plastid transformation system in liverwort, we disrupted chlB, which encodes a subunit of DPOR in the M. polymorpha chloroplast genome. Morphological and Chl content analysis of a chlB mutant grown under different photoperiods revealed that DPOR is particularly required for Chl biosynthesis under short-day conditions. Our findings suggest that an environmental condition in the form of photoperiod is an important factor that determines the loss or retention of chloroplast-encoded genes mediating Pchlide reduction to Chlide.},
doi = {10.1093/gbe/evu045},
journal = {Genome Biology and Evolution},
number = 3,
volume = 6,
place = {United States},
year = {Thu Feb 27 00:00:00 EST 2014},
month = {Thu Feb 27 00:00:00 EST 2014}
}
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FLU, a negative feedback regulator of tetrapyrrole biosynthesis, is physically linked to the final steps of the Mg ++ -branch of this pathway
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Directed disruption of the tobacco ndhB gene impairs cyclic electron flow around photosystem I
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Chloroplast Phylogenomics Indicates that Ginkgo biloba Is Sister to Cycads
journal, January 2013
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Identification of the chlB Gene and the Gene Product Essential for the Light-Independent Chlorophyll Biosynthesis in the Cyanobacterium Plectonema boryanum
journal, April 1996
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Characterization of cDNA of the Liverwort Phytochrome Gene, and Phytochrome Involvement in the Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductase Gene Expression in Marchantia paleacea var. diptera
journal, June 2001
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COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS
journal, January 1949
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Rapid Induction of Distinct Stress Responses after the Release of Singlet Oxygen in Arabidopsis
journal, October 2003
- op den Camp, R. G. L.
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Identification of a Vinyl Reductase Gene for Chlorophyll Synthesis in Arabidopsis thaliana and Implications for the Evolution of Prochlorococcus Species
journal, January 2005
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DELLAs Regulate Chlorophyll and Carotenoid Biosynthesis to Prevent Photooxidative Damage during Seedling Deetiolation in Arabidopsis
journal, May 2011
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Tetrapyrrole Biosynthesis in Higher Plants
journal, June 2007
- Tanaka, Ryouichi; Tanaka, Ayumi
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Decoding of Light Signals by Plant Phytochromes and Their Interacting Proteins
journal, June 2008
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Light-Independent Chlorophyll Biosynthesis: Involvement of the Chloroplast Gene chlL (frxC)
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Chloroplast-Encoded chlB Is Required for Light-Independent Protochlorophyllide Reductase Activity in Chlamydomonas reinhardtii
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