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Title: A data set from flash X-ray imaging of carboxysomes

Journal Article · · Scientific Data
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  1. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; DOE/OSTI
  2. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics
  3. National University of Singapore (Singapore). Centre for BioImaging Sciences
  4. Univ. of Melbourne (Australia). ARC Centre of Excellence for Advanced Molecular Imaging. School of Physics
  5. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Uppsala Univ. (Sweden). Dept. of Physics and Astronomy
  6. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Academy of Sciences of the Czech Republic, Prague (Czech Republic). Inst. of Physics. ELI beamlines
  7. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  8. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Tor Vergata Univ. of Rome (Italy). Physics Dept. INFN
  9. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  10. European X-ray Free-Electron Laser (XFEL), Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  11. PNSensor GmbH, München (Germany)
  12. Max Planck Inst. fuer Extraterrestrische Physik, Garching (Germany)
  13. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  14. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Kansas State Univ., Manhattan, KS (United States). J.R. Macdonald Lab. Dept. of Physics; Max Planck Inst. for Medical Research, Heidelberg (Germany); Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Hamburg (Germany)
  15. Kansas State Univ., Manhattan, KS (United States). J.R. Macdonald Lab. Dept. of Physics; Max Planck Inst. for Medical Research, Heidelberg (Germany); Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Hamburg (Germany); Max Planck Inst. fuer Kernphysik, Heidelberg (Germany)
  16. Kansas State Univ., Manhattan, KS (United States). J.R. Macdonald Lab. Dept. of Physics; Max Planck Inst. for Medical Research, Heidelberg (Germany)
  17. Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Hamburg (Germany); Max Planck Inst. fuer Kernphysik, Heidelberg (Germany); Max Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany)
  18. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; European X-ray Free-Electron Laser (XFEL), Hamburg (Germany)
  19. Uppsala Univ. (Sweden). Dept. of Cell and Molecular Biology, Lab. of Molecular Biophysics; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
+ Show Author Affiliations

Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115 ±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Rocky Mountain Oilfield Testing Center (RMOTC), Casper, WY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1624548
Journal Information:
Scientific Data, Journal Name: Scientific Data Journal Issue: 1 Vol. 3; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (9)

Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene journal May 2018
Coherent diffractive imaging of microtubules using an X-ray laser journal June 2019
The trickle before the torrent—diffraction data from X-ray lasers journal August 2016
Short-wavelength free-electron laser sources and science: a review journal October 2017
Short-wavelength free-electron laser sources and science: a review text January 2017
Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene text January 2018
Coherent diffractive imaging of microtubules using an X-ray laser text January 2019
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019

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