Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments

McKay, Luke J.; Hatzenpichler, Roland; Inskeep, William P.; Fields, Matthew W.

doi:10.1038/s41598-017-07354-x

Title: Occurrence and expression of novel methyl-coenzyme M reductase gene (mcrA) variants in hot spring sediments

Journal Article · Thu Aug 03 00:00:00 UTC 2017 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-017-07354-x · OSTI ID:1624317

McKay, Luke J. ^[1]; Hatzenpichler, Roland ^[2]; Inskeep, William P. ^[3]; Fields, Matthew W. ^[4]

Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering; Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences; DOE/OSTI
Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering; Montana State Univ., Bozeman, MT (United States). Dept. of Chemistry and Biochemistry
Montana State Univ., Bozeman, MT (United States). Dept. of Land Resources and Environmental Sciences
Montana State Univ., Bozeman, MT (United States). Center for Biofilm Engineering; Montana State Univ., Bozeman, MT (United States). Dept. of Microbiology and Immunology

Recent discoveries have shown that the marker gene for anaerobic methane cycling (mcrA) is more widespread in the Archaea than previously thought. However, it remains unclear whether novel mcrA genes associated with the Bathyarchaeota and Verstraetearchaeota are distributed across diverse environments. We examined two geochemically divergent but putatively methanogenic regions of Yellowstone National Park to investigate whether deeply-rooted archaea possess and express novel mcrA genes in situ. Small-subunit (SSU) rRNA gene analyses indicated that Bathyarchaeota were predominant in seven of ten sediment layers, while the Verstraetearchaeota and Euryarchaeota occurred in lower relative abundance. Targeted amplification of novel mcrA genes suggested that diverse taxa contribute to alkane cycling in geothermal environments. Two deeply-branching mcrA clades related to Bathyarchaeota were identified, while highly abundant verstraetearchaeotal mcrA sequences were also recovered. In addition, detection of SSU rRNA and mcrA transcripts from one hot spring suggested that predominant Bathyarchaeota were also active, and that methane cycling genes are expressed by the Euryarchaeota, Verstraetearchaeota, and an unknown lineage basal to the Bathyarchaeota. These findings greatly expand the diversity of the key marker gene for anaerobic alkane cycling and outline the need for greater understanding of the functional capacity and phylogenetic affiliation of novel mcrA variants.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1624317

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Microbial Community Structure and Methane Cycling Potential along a Thermokarst Pond-Peatland Continuum Vigneron, Adrien; Cruaud, Perrine; Bhiry, Najat Microorganisms, Vol. 7, Issue 11 https://doi.org/10.3390/microorganisms7110486	journal	October 2019

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