Giant virus diversity and host interactions through global metagenomics
Abstract
Our current knowledge about nucleocytoplasmic large DNA viruses (NCLDVs) is largely derived from viral isolates that are co-cultivated with protists and algae. Here we reconstructed 2,074 NCLDV genomes from sampling sites across the globe by building on the rapidly increasing amount of publicly available metagenome data. This led to an 11-fold increase in phylogenetic diversity and a parallel 10-fold expansion in functional diversity. Analysis of 58,023 major capsid proteins from large and giant viruses using metagenomic data revealed the global distribution patterns and cosmopolitan nature of these viruses. The discovered viral genomes encoded a wide range of proteins with putative roles in photosynthesis and diverse substrate transport processes, indicating that host reprogramming is probably a common strategy in the NCLDVs. Furthermore, inferences of horizontal gene transfer connected viral lineages to diverse eukaryotic hosts. We anticipate that the global diversity of NCLDVs that we describe here will establish giant viruses-which are associated with most major eukaryotic lineages-as important players in ecosystems across Earth's biomes.
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint Genome Institute
- Concordia University, Québec (Canada), Groupe de recherche interuniversitaire en limnologie, Dept. of Biology
- Univ. of Michigan, Ann Arbor, MI (United States). Dept. of Ecology and Evolutionary Biology
- Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology; Univ. of Wisconsin, Madison, WI (United States). Dept. Civil and Environmental Engineering
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Civil and Environmental Engineering
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1624270
- Alternate Identifier(s):
- OSTI ID: 1760213
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature (London)
- Additional Journal Information:
- Journal Name: Nature (London); Journal Volume: 578; Journal Issue: 7795; Journal ID: ISSN 0028-0836
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Biodiversity; environmental microbiology; evolution; viral evolution; virus-host interactions
Citation Formats
Schulz, Frederik, Roux, Simon, Paez-Espino, David, Jungbluth, Sean, Walsh, David A., Denef, Vincent J., McMahon, Katherine D., Konstantinidis, Konstantinos T., Eloe-Fadrosh, Emiley A., Kyrpides, Nikos C., and Woyke, Tanja. Giant virus diversity and host interactions through global metagenomics. United States: N. p., 2020.
Web. doi:10.1038/s41586-020-1957-x.
Schulz, Frederik, Roux, Simon, Paez-Espino, David, Jungbluth, Sean, Walsh, David A., Denef, Vincent J., McMahon, Katherine D., Konstantinidis, Konstantinos T., Eloe-Fadrosh, Emiley A., Kyrpides, Nikos C., & Woyke, Tanja. Giant virus diversity and host interactions through global metagenomics. United States. https://doi.org/10.1038/s41586-020-1957-x
Schulz, Frederik, Roux, Simon, Paez-Espino, David, Jungbluth, Sean, Walsh, David A., Denef, Vincent J., McMahon, Katherine D., Konstantinidis, Konstantinos T., Eloe-Fadrosh, Emiley A., Kyrpides, Nikos C., and Woyke, Tanja. Wed .
"Giant virus diversity and host interactions through global metagenomics". United States. https://doi.org/10.1038/s41586-020-1957-x. https://www.osti.gov/servlets/purl/1624270.
@article{osti_1624270,
title = {Giant virus diversity and host interactions through global metagenomics},
author = {Schulz, Frederik and Roux, Simon and Paez-Espino, David and Jungbluth, Sean and Walsh, David A. and Denef, Vincent J. and McMahon, Katherine D. and Konstantinidis, Konstantinos T. and Eloe-Fadrosh, Emiley A. and Kyrpides, Nikos C. and Woyke, Tanja},
abstractNote = {Our current knowledge about nucleocytoplasmic large DNA viruses (NCLDVs) is largely derived from viral isolates that are co-cultivated with protists and algae. Here we reconstructed 2,074 NCLDV genomes from sampling sites across the globe by building on the rapidly increasing amount of publicly available metagenome data. This led to an 11-fold increase in phylogenetic diversity and a parallel 10-fold expansion in functional diversity. Analysis of 58,023 major capsid proteins from large and giant viruses using metagenomic data revealed the global distribution patterns and cosmopolitan nature of these viruses. The discovered viral genomes encoded a wide range of proteins with putative roles in photosynthesis and diverse substrate transport processes, indicating that host reprogramming is probably a common strategy in the NCLDVs. Furthermore, inferences of horizontal gene transfer connected viral lineages to diverse eukaryotic hosts. We anticipate that the global diversity of NCLDVs that we describe here will establish giant viruses-which are associated with most major eukaryotic lineages-as important players in ecosystems across Earth's biomes.},
doi = {10.1038/s41586-020-1957-x},
journal = {Nature (London)},
number = 7795,
volume = 578,
place = {United States},
year = {Wed Jan 22 00:00:00 EST 2020},
month = {Wed Jan 22 00:00:00 EST 2020}
}
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