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Title: Non-contact electrochemical evaluation of biofilms

Abstract

Abstract Here, we demonstrate a non-contact technique for electrochemical evaluation of biofilms on surfaces in relation to corrosion. Electrochemical impedance spectrometry was employed, incorporating flat patterned electrodes positioned over the surfaces of aluminum and glass with and without biofilms. Signal communication from the working electrode to the counter electrode followed electric field lines passing tangentially through the biofilms. Electrochemical impedance parameters that were evaluated included complex impedance, phase angle, imaginary (out of phase) conductivity and Cole–Cole plots with a corresponding equivalent circuit. Changes in the impedance properties due to the presence of biofilms were monitored and correlated through microbiological, chemical and electrochemical assays. Impedance parameters associated with microbial activity correlated with biofilms on aluminum and glass surfaces. This technical approach provides impedance information about the biofilm without the signal traveling through the underlying conductive media or disrupting the biofilm. In this way, biological contributions to surface fouling can be evaluated with minimal contribution from the inorganic surface under the biofilm. In addition, this technique can be used to monitor biofilms on electrochemically inert surfaces as well as electrically conductive surfaces. Graphic abstract

Authors:
ORCiD logo; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1619436
Grant/Contract Number:  
AC09-08SR22470
Resource Type:
Published Article
Journal Name:
SN Applied Sciences
Additional Journal Information:
Journal Name: SN Applied Sciences Journal Volume: 2 Journal Issue: 3; Journal ID: ISSN 2523-3963
Publisher:
Springer Science + Business Media
Country of Publication:
Switzerland
Language:
English

Citation Formats

Turick, Charles E., Colon-Mercado, Hector, Bagwell, Christopher E., Greenway, Scott D., and Amoroso, Jake W. Non-contact electrochemical evaluation of biofilms. Switzerland: N. p., 2020. Web. doi:10.1007/s42452-020-2081-0.
Turick, Charles E., Colon-Mercado, Hector, Bagwell, Christopher E., Greenway, Scott D., & Amoroso, Jake W. Non-contact electrochemical evaluation of biofilms. Switzerland. doi:https://doi.org/10.1007/s42452-020-2081-0
Turick, Charles E., Colon-Mercado, Hector, Bagwell, Christopher E., Greenway, Scott D., and Amoroso, Jake W. Wed . "Non-contact electrochemical evaluation of biofilms". Switzerland. doi:https://doi.org/10.1007/s42452-020-2081-0.
@article{osti_1619436,
title = {Non-contact electrochemical evaluation of biofilms},
author = {Turick, Charles E. and Colon-Mercado, Hector and Bagwell, Christopher E. and Greenway, Scott D. and Amoroso, Jake W.},
abstractNote = {Abstract Here, we demonstrate a non-contact technique for electrochemical evaluation of biofilms on surfaces in relation to corrosion. Electrochemical impedance spectrometry was employed, incorporating flat patterned electrodes positioned over the surfaces of aluminum and glass with and without biofilms. Signal communication from the working electrode to the counter electrode followed electric field lines passing tangentially through the biofilms. Electrochemical impedance parameters that were evaluated included complex impedance, phase angle, imaginary (out of phase) conductivity and Cole–Cole plots with a corresponding equivalent circuit. Changes in the impedance properties due to the presence of biofilms were monitored and correlated through microbiological, chemical and electrochemical assays. Impedance parameters associated with microbial activity correlated with biofilms on aluminum and glass surfaces. This technical approach provides impedance information about the biofilm without the signal traveling through the underlying conductive media or disrupting the biofilm. In this way, biological contributions to surface fouling can be evaluated with minimal contribution from the inorganic surface under the biofilm. In addition, this technique can be used to monitor biofilms on electrochemically inert surfaces as well as electrically conductive surfaces. Graphic abstract},
doi = {10.1007/s42452-020-2081-0},
journal = {SN Applied Sciences},
number = 3,
volume = 2,
place = {Switzerland},
year = {2020},
month = {2}
}

Journal Article:
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DOI: https://doi.org/10.1007/s42452-020-2081-0

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Works referenced in this record:

AC-Impedance Measurements on Corroded Porous Aluminum Oxide Films
journal, January 1986

  • Hitzig, J.
  • Journal of The Electrochemical Society, Vol. 133, Issue 5
  • DOI: 10.1149/1.2108756

Effect of bioclogging in porous media on complex conductivity signatures
journal, January 2010

  • Abdel Aal, Gamal Z.; Atekwana, Estella A.; Atekwana, Eliot A.
  • Journal of Geophysical Research, Vol. 115
  • DOI: 10.1029/2009JG001159

Complex Dielectric Properties of Sulfate-Reducing Bacteria Suspensions
journal, July 2013


Rapid bacterial detection with an interdigitated array electrode by electrochemical impedance spectroscopy
journal, November 2012


Microbiologically influenced corrosion of NiZn alloy coatings by Delftia acidovorans bacterium
journal, November 2012


Low-frequency, low-field dielectric spectroscopy of living cell suspensions
journal, April 2004

  • Prodan, C.; Mayo, F.; Claycomb, J. R.
  • Journal of Applied Physics, Vol. 95, Issue 7
  • DOI: 10.1063/1.1649455

Attached and Unattached Microbial Communities in a Simulated Basalt Aquifer under Fracture- and Porous-Flow Conditions
journal, June 2001


Investigations of structure and metabolism within Shewanella oneidensis MR-1 biofilms
journal, July 2008

  • McLean, Jeffrey S.; Majors, Paul D.; Reardon, Catherine L.
  • Journal of Microbiological Methods, Vol. 74, Issue 1
  • DOI: 10.1016/j.mimet.2008.02.015

Microbial Corrosion of Aluminum 2024 Aeronautical Alloy by Hydrocarbon Degrading Bacteria Bacillus cereus ACE4 and Serratia marcescens ACE2
journal, July 2010

  • Rajasekar, Aruliah; Ting, Yen-Peng
  • Industrial & Engineering Chemistry Research, Vol. 49, Issue 13
  • DOI: 10.1021/ie100078u

Use of nuclepore filters for counting bacteria by fluorescence microscopy.
journal, January 1977


Electrochemical and surface analytical techniques applied to microbiologically influenced corrosion investigation
journal, July 2018


In-situ detection based on the biofilm hydrophilicity for environmental biofilm formation
journal, May 2019


Microbial Biofilm Voltammetry: Direct Electrochemical Characterization of Catalytic Electrode-Attached Biofilms
journal, October 2008

  • Marsili, E.; Rollefson, J. B.; Baron, D. B.
  • Applied and Environmental Microbiology, Vol. 74, Issue 23
  • DOI: 10.1128/AEM.00177-08

Electric double layer interactions in bacterial adhesion to surfaces
journal, June 2002


Natural polysaccharides as electroactive polymers
journal, February 2005


Microbiology of Spent Nuclear Fuel Storage Basins
journal, December 1998

  • Santo Domingo, Jorge W.; Berry, Christopher J.; Summer, Michael
  • Current Microbiology, Vol. 37, Issue 6
  • DOI: 10.1007/s002849900398

Amplicon Sequencing Reveals Microbiological Signatures in Spent Nuclear Fuel Storage Basins
journal, March 2018

  • Bagwell, Christopher E.; Noble, Peter A.; Milliken, Charles E.
  • Frontiers in Microbiology, Vol. 9
  • DOI: 10.3389/fmicb.2018.00377

The dielectric properties of biological cells at radiofrequencies: applications in biotechnology
journal, August 1999


An investigation of the formation and destruction of corrosion inhibitor films using electrochemical impedance spectroscopy (EIS)
journal, September 1996


Corrosion inhibition of mild steel by aerobic biofilm
journal, August 2005


In situ determination of the loss of adhesion of barrier epoxy coatings using electrochemical impedance spectroscopy
journal, October 1993


Microbially influenced corrosion of stainless steel by marine bacterium Vibrio natriegens: (I) Corrosion behavior
journal, April 2009


Microbial biofilm growth on irradiated, spent nuclear fuel cladding
journal, February 2009


SIP response of artificial biofilms
journal, January 2009

  • Ntarlagiannis, Dimitrios; Ferguson, Andrew
  • GEOPHYSICS, Vol. 74, Issue 1
  • DOI: 10.1190/1.3031514

Plasmon-Waveguide Resonance and Impedance Spectroscopy Studies of the Interaction between Penetratin and Supported Lipid Bilayer Membranes
journal, March 2003