High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India
Abstract
Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618967
- Alternate Identifier(s):
- OSTI ID: 1379405
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Standards in Genomic Sciences
- Additional Journal Information:
- Journal Name: Standards in Genomic Sciences Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1944-3277
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; root-nodule bacteria; nitrogen fixation; symbiosis; ensifer; prosopis
Citation Formats
Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India. United Kingdom: N. p., 2016.
Web. doi:10.1186/s40793-016-0157-7.
Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, & Reeve, Wayne. High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India. United Kingdom. https://doi.org/10.1186/s40793-016-0157-7
Gehlot, Hukam Singh, Ardley, Julie, Tak, Nisha, Tian, Rui, Poonar, Neetu, Meghwal, Raju R., Rathi, Sonam, Tiwari, Ravi, Adnawani, Wan, Seshadri, Rekha, Reddy, T. B. K., Pati, Amrita, Woyke, Tanja, Pillay, Manoj, Markowitz, Victor, Baeshen, Mohammed N., Al-Hejin, Ahmed M., Ivanova, Natalia, Kyrpides, Nikos, and Reeve, Wayne. Thu .
"High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India". United Kingdom. https://doi.org/10.1186/s40793-016-0157-7.
@article{osti_1618967,
title = {High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India},
author = {Gehlot, Hukam Singh and Ardley, Julie and Tak, Nisha and Tian, Rui and Poonar, Neetu and Meghwal, Raju R. and Rathi, Sonam and Tiwari, Ravi and Adnawani, Wan and Seshadri, Rekha and Reddy, T. B. K. and Pati, Amrita and Woyke, Tanja and Pillay, Manoj and Markowitz, Victor and Baeshen, Mohammed N. and Al-Hejin, Ahmed M. and Ivanova, Natalia and Kyrpides, Nikos and Reeve, Wayne},
abstractNote = {Ensifer sp. PC2 is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a nitrogen-fixing nodule of the tree legume P. cineraria (L.) Druce (Khejri), which is a keystone species that grows in arid and semi-arid regions of the Indian Thar desert. Strain PC2 exists as a dominant saprophyte in alkaline soils of Western Rajasthan. It is fast growing, well-adapted to arid conditions and is able to form an effective symbiosis with several annual crop legumes as well as species of mimosoid trees and shrubs. Here we describe the features of Ensifer sp. PC2, together with genome sequence information and its annotation. The 8,458,965 bp high-quality permanent draft genome is arranged into 171 scaffolds of 171 contigs containing 8,344 protein-coding genes and 139 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.},
doi = {10.1186/s40793-016-0157-7},
journal = {Standards in Genomic Sciences},
number = 1,
volume = 11,
place = {United Kingdom},
year = {Thu Jun 23 00:00:00 EDT 2016},
month = {Thu Jun 23 00:00:00 EDT 2016}
}
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https://doi.org/10.1186/s40793-016-0157-7
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Cited by: 8 works
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Figures / Tables:
Fig. 1 : Images of Ensifer sp. PC2 using scanning (Left) and transmission (Center) electron microscopy and the appearance of colony morphology on solid media (Right)
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Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.