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Title: Methanobactin from methanotrophs: genetics, structure, function and potential applications

Abstract

ABSTRACT Aerobic methane-oxidizing bacteria of the Alphaproteobacteria have been found to express a novel ribosomally synthesized post-translationally modified polypeptide (RiPP) termed methanobactin (MB). The primary function of MB in these microbes appears to be for copper uptake, but MB has been shown to have multiple capabilities, including oxidase, superoxide dismutase and hydrogen peroxide reductase activities, the ability to detoxify mercury species, as well as acting as an antimicrobial agent. Herein, we describe the diversity of known MBs as well as the genetics underlying MB biosynthesis. We further propose based on bioinformatics analyses that some methanotrophs may produce novel forms of MB that have yet to be characterized. We also discuss recent findings documenting that MBs play an important role in controlling copper availability to the broader microbial community, and as a result can strongly affect the activity of microbes that require copper for important enzymatic transformations, e.g. conversion of nitrous oxide to dinitrogen. Finally, we describe procedures for the detection/purification of MB, as well as potential medical and industrial applications of this intriguing RiPP.

Authors:
 [1];  [2];  [1];  [1]
  1. Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, MI, USA 48109-2125
  2. Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, USA 50011
Publication Date:
Research Org.:
Univ. of Michigan, Ann Arbor, MI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
OSTI Identifier:
1615161
Alternate Identifier(s):
OSTI ID: 1691490
Grant/Contract Number:  
SC0018059; SC0020174; 1724744; 1912482
Resource Type:
Published Article
Journal Name:
FEMS Microbiology Letters (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Letters (Online) Journal Volume: 367 Journal Issue: 5; Journal ID: ISSN 1574-6968
Publisher:
Federation of European Microbiological Societies
Country of Publication:
Netherlands
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; methanobactin; methanotroph; mercury; methylmercury; copper

Citation Formats

Semrau, Jeremy D., DiSpirito, Alan A., Obulisamy, Parthiba Karthikeyan, and Kang-Yun, Christina S. Methanobactin from methanotrophs: genetics, structure, function and potential applications. Netherlands: N. p., 2020. Web. doi:10.1093/femsle/fnaa045.
Semrau, Jeremy D., DiSpirito, Alan A., Obulisamy, Parthiba Karthikeyan, & Kang-Yun, Christina S. Methanobactin from methanotrophs: genetics, structure, function and potential applications. Netherlands. doi:https://doi.org/10.1093/femsle/fnaa045
Semrau, Jeremy D., DiSpirito, Alan A., Obulisamy, Parthiba Karthikeyan, and Kang-Yun, Christina S. Fri . "Methanobactin from methanotrophs: genetics, structure, function and potential applications". Netherlands. doi:https://doi.org/10.1093/femsle/fnaa045.
@article{osti_1615161,
title = {Methanobactin from methanotrophs: genetics, structure, function and potential applications},
author = {Semrau, Jeremy D. and DiSpirito, Alan A. and Obulisamy, Parthiba Karthikeyan and Kang-Yun, Christina S.},
abstractNote = {ABSTRACT Aerobic methane-oxidizing bacteria of the Alphaproteobacteria have been found to express a novel ribosomally synthesized post-translationally modified polypeptide (RiPP) termed methanobactin (MB). The primary function of MB in these microbes appears to be for copper uptake, but MB has been shown to have multiple capabilities, including oxidase, superoxide dismutase and hydrogen peroxide reductase activities, the ability to detoxify mercury species, as well as acting as an antimicrobial agent. Herein, we describe the diversity of known MBs as well as the genetics underlying MB biosynthesis. We further propose based on bioinformatics analyses that some methanotrophs may produce novel forms of MB that have yet to be characterized. We also discuss recent findings documenting that MBs play an important role in controlling copper availability to the broader microbial community, and as a result can strongly affect the activity of microbes that require copper for important enzymatic transformations, e.g. conversion of nitrous oxide to dinitrogen. Finally, we describe procedures for the detection/purification of MB, as well as potential medical and industrial applications of this intriguing RiPP.},
doi = {10.1093/femsle/fnaa045},
journal = {FEMS Microbiology Letters (Online)},
number = 5,
volume = 367,
place = {Netherlands},
year = {2020},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
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DOI: https://doi.org/10.1093/femsle/fnaa045

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