skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1605607
Grant/Contract Number:  
[SC0015662]
Resource Type:
Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
[Journal Name: Frontiers in Microbiology Journal Volume: 11]; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Media SA
Country of Publication:
Switzerland
Language:
English

Citation Formats

Sacher, Jessica C., Shajahan, Asif, Butcher, James, Patry, Robert T., Flint, Annika, Hendrixson, David R., Stintzi, Alain, Azadi, Parastoo, and Szymanski, Christine M. Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans. Switzerland: N. p., 2020. Web. doi:10.3389/fmicb.2020.00397.
Sacher, Jessica C., Shajahan, Asif, Butcher, James, Patry, Robert T., Flint, Annika, Hendrixson, David R., Stintzi, Alain, Azadi, Parastoo, & Szymanski, Christine M. Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans. Switzerland. doi:10.3389/fmicb.2020.00397.
Sacher, Jessica C., Shajahan, Asif, Butcher, James, Patry, Robert T., Flint, Annika, Hendrixson, David R., Stintzi, Alain, Azadi, Parastoo, and Szymanski, Christine M. Fri . "Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans". Switzerland. doi:10.3389/fmicb.2020.00397.
@article{osti_1605607,
title = {Binding of Phage-Encoded FlaGrab to Motile Campylobacter jejuni Flagella Inhibits Growth, Downregulates Energy Metabolism, and Requires Specific Flagellar Glycans},
author = {Sacher, Jessica C. and Shajahan, Asif and Butcher, James and Patry, Robert T. and Flint, Annika and Hendrixson, David R. and Stintzi, Alain and Azadi, Parastoo and Szymanski, Christine M.},
abstractNote = {},
doi = {10.3389/fmicb.2020.00397},
journal = {Frontiers in Microbiology},
number = ,
volume = [11],
place = {Switzerland},
year = {2020},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.3389/fmicb.2020.00397

Save / Share:

Works referenced in this record:

The MotA protein of E. coli is a proton-conducting component of the flagellar motor
journal, February 1990


Identification of the Carbohydrate Moieties and Glycosylation Motifs in Campylobacter jejuni Flagellin
journal, July 2001

  • Thibault, Pierre; Logan, Susan M.; Kelly, John F.
  • Journal of Biological Chemistry, Vol. 276, Issue 37
  • DOI: 10.1074/jbc.M104529200

GAGE: generally applicable gene set enrichment for pathway analysis
journal, January 2009


Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa
journal, April 2004


Phage typing of Campylobacter jejuni and Campylobacter coli and its use as an adjunct to serotyping
journal, August 1999

  • Frost, J. A.; Kramer, J. M.; Gillanders, S. A.
  • Epidemiology and Infection, Vol. 123, Issue 1
  • DOI: 10.1017/S095026889900254X

Pseudaminic acid, the major modification on Campylobacter flagellin, is synthesized via the Cj1293 gene: Campylobacter flagellin glycosylation
journal, October 2003


Phase Variable Expression of a Single Phage Receptor in Campylobacter jejuni NCTC12662 Influences Sensitivity Toward Several Diverse CPS-Dependent Phages
journal, February 2018

  • Gencay, Yilmaz Emre; Sørensen, Martine C. H.; Wenzel, Cory Q.
  • Frontiers in Microbiology, Vol. 9
  • DOI: 10.3389/fmicb.2018.00082

Campylobacter sugars sticking out
journal, September 2008


Protein glycosylation in bacterial mucosal pathogens
journal, March 2005

  • Szymanski, Christine M.; Wren, Brendan W.
  • Nature Reviews Microbiology, Vol. 3, Issue 3
  • DOI: 10.1038/nrmicro1100

STAR: ultrafast universal RNA-seq aligner
journal, October 2012


Phase Variable Expression of Capsular Polysaccharide Modifications Allows Campylobacter jejuni to Avoid Bacteriophage Infection in Chickens
journal, January 2012

  • Sørensen, Martine C. Holst; van Alphen, Lieke B.; Fodor, Christopher
  • Frontiers in Cellular and Infection Microbiology, Vol. 2
  • DOI: 10.3389/fcimb.2012.00011

Genome and Proteome of Campylobacter jejuni Bacteriophage NCTC 12673
journal, September 2011

  • Kropinski, Andrew M.; Arutyunov, Denis; Foss, Mary
  • Applied and Environmental Microbiology, Vol. 77, Issue 23
  • DOI: 10.1128/AEM.05562-11

A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis : Flagella-mediated signal transduction
journal, August 2013

  • Cairns, Lynne S.; Marlow, Victoria L.; Bissett, Emma
  • Molecular Microbiology
  • DOI: 10.1111/mmi.12342

The impairment of methylmenaquinol:fumarate reductase affects hydrogen peroxide susceptibility and accumulation in Campylobacter jejuni
journal, February 2014

  • Kassem, Issmat I.; Khatri, Mahesh; Sanad, Yasser M.
  • MicrobiologyOpen, Vol. 3, Issue 2
  • DOI: 10.1002/mbo3.158

Identification of Unusual Bacterial Glycosylation by Tandem Mass Spectrometry Analyses of Intact Proteins
journal, December 2005

  • Schirm, Michael; Schoenhofen, Ian C.; Logan, Susan M.
  • Analytical Chemistry, Vol. 77, Issue 23
  • DOI: 10.1021/ac051316y

Exploring the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates
journal, October 2015

  • Simpson, David J.; Sacher, Jessica C.; Szymanski, Christine M.
  • Current Opinion in Structural Biology, Vol. 34
  • DOI: 10.1016/j.sbi.2015.07.006

Campylobacter virulence and survival factors
journal, June 2015


The Escherichia coli MotAB Proton Channel Unplugged
journal, December 2006

  • Hosking, Edan R.; Vogt, Christian; Bakker, Evert P.
  • Journal of Molecular Biology, Vol. 364, Issue 5
  • DOI: 10.1016/j.jmb.2006.09.035

Development and Application of an Insertional System for Gene Delivery and Expression in Campylobacter jejuni
journal, July 2005


Proton-conductivity assay of plugged and unplugged MotA/B proton channel by cytoplasmic pHluorin expressed in Salmonella
journal, February 2010


Flagellar glycosylation - a new component of the motility repertoire?
journal, May 2006


Biofilms, flagella, and mechanosensing of surfaces by bacteria
journal, September 2014


Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold
journal, March 2016

  • Beeby, Morgan; Ribardo, Deborah A.; Brennan, Caitlin A.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 13
  • DOI: 10.1073/pnas.1518952113

Campylobacter jejuni motility and invasion of Caco-2 cells.
journal, January 1995


Bacteriophage Receptor Binding Protein Based Assays for the Simultaneous Detection of Campylobacter jejuni and Campylobacter coli
journal, July 2013


High Throughput Method for Analysis of Repeat Number for 28 Phase Variable Loci of Campylobacter jejuni Strain NCTC11168
journal, July 2016


Iron Acquisition and Regulation in Campylobacter jejuni
journal, July 2004


Campylobacter jejuni transcriptional and genetic adaptation during human infection
journal, March 2018

  • Crofts, Alexander A.; Poly, Frédéric M.; Ewing, Cheryl P.
  • Nature Microbiology, Vol. 3, Issue 4
  • DOI: 10.1038/s41564-018-0133-7

Bacteriophage F336 Recognizes the Capsular Phosphoramidate Modification of Campylobacter jejuni NCTC11168
journal, September 2011

  • Sorensen, M. C. H.; van Alphen, L. B.; Harboe, A.
  • Journal of Bacteriology, Vol. 193, Issue 23
  • DOI: 10.1128/JB.05276-11

Modification of the Campylobacter jejuni flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene
journal, July 2010


A Flagellar Glycan-Specific Protein Encoded by Campylobacter Phages Inhibits Host Cell Growth
journal, December 2015

  • Javed, Muhammad; Sacher, Jessica; van Alphen, Lieke
  • Viruses, Vol. 7, Issue 12
  • DOI: 10.3390/v7122964

Changes in flagellin glycosylation affect Campylobacter autoagglutination and virulence
journal, April 2006


Transcriptomic Analysis of the Campylobacter jejuni Response to T4-Like Phage NCTC 12673 Infection
journal, June 2018

  • Sacher, Jessica; Flint, Annika; Butcher, James
  • Viruses, Vol. 10, Issue 6
  • DOI: 10.3390/v10060332

Complete Genome Sequence of Campylobacter jejuni Strain 12567, a Livestock-Associated Clade Representative
journal, June 2018


A functional Campylobacter jejuni maf4 gene results in novel glycoforms on flagellin and altered autoagglutination behaviour
journal, November 2008


A novel paralogous gene family involved in phase-variable flagella-mediated motility in Campylobacter jejuni
journal, February 2002


Novel Glycosylation Sites Localized in Campylobacter jejuni Flagellin FlaA by Liquid Chromatography Electron Capture Dissociation Tandem Mass Spectrometry
journal, March 2011

  • Zampronio, Cleidiane G.; Blackwell, Gemma; Penn, Charles W.
  • Journal of Proteome Research, Vol. 10, Issue 3
  • DOI: 10.1021/pr101021c

Mucosal Reactive Oxygen Species Decrease Virulence by Disrupting Campylobacter jejuni Phosphotyrosine Signaling
journal, July 2012

  • Corcionivoschi, Nicolae; Alvarez, Luis A. J.; Sharp, Thomas H.
  • Cell Host & Microbe, Vol. 12, Issue 1
  • DOI: 10.1016/j.chom.2012.05.018

Epidemiology and Impact of Campylobacter Infection in Children in 8 Low-Resource Settings: Results From the MAL-ED Study
journal, August 2016

  • Amour, Caroline; Gratz, Jean; Mduma, Estomih
  • Clinical Infectious Diseases
  • DOI: 10.1093/cid/ciw542

Evolution of higher torque in Campylobacter-type bacterial flagellar motors
journal, January 2018


Structural heterogeneity of carbohydrate modifications affects serospecificity of Campylobacter flagellins: Glycosylation of Campylobacter flagellin
journal, October 2002


Functional Characterization of the Flagellar Glycosylation Locus in Campylobacter jejuni 81–176 Using a Focused Metabolomics Approach
journal, May 2006

  • McNally, David J.; Hui, Joseph P. M.; Aubry, Annie J.
  • Journal of Biological Chemistry, Vol. 281, Issue 27
  • DOI: 10.1074/jbc.M603777200

Targeting virulence not viability in the search for future antibacterials: Antivirulence strategies for bacterial infection
journal, January 2015

  • Heras, Begoña; Scanlon, Martin J.; Martin, Jennifer L.
  • British Journal of Clinical Pharmacology, Vol. 79, Issue 2
  • DOI: 10.1111/bcp.12356

A Chaperone for the Stator Units of a Bacterial Flagellum
journal, August 2019

  • Ribardo, Deborah A.; Kelley, Brittni R.; Johnson, Jeremiah G.
  • mBio, Vol. 10, Issue 4
  • DOI: 10.1128/mBio.01732-19

A suggested classification for two groups of Campylobacter myoviruses
journal, July 2013

  • Javed, Muhammad Afzal; Ackermann, Hans-Wolfgang; Azeredo, Joana
  • Archives of Virology, Vol. 159, Issue 1
  • DOI: 10.1007/s00705-013-1788-2

Complete Genome Sequences of Three Campylobacter jejuni Phage-Propagating Strains
journal, June 2018


Campylobacter flagella: not just for motility
journal, October 2007


Campylobacter jejuni Motility Is Required for Infection of the Flagellotropic Bacteriophage F341
journal, September 2014

  • Baldvinsson, Signe Berg; Sørensen, Martine C. Holst; Vegge, Christina S.
  • Applied and Environmental Microbiology, Vol. 80, Issue 22
  • DOI: 10.1128/AEM.02057-14

Proteomic Analysis of Campylobacter jejuni 11168 Biofilms Reveals a Role for the Motility Complex in Biofilm Formation
journal, June 2006

  • Kalmokoff, M.; Lanthier, P.; Tremblay, T. -L.
  • Journal of Bacteriology, Vol. 188, Issue 12
  • DOI: 10.1128/JB.01975-05

Functional Characterization of Dehydratase/Aminotransferase Pairs from Helicobacter and Campylobacter
journal, November 2005

  • Schoenhofen, Ian C.; McNally, David J.; Vinogradov, Evgeny
  • Journal of Biological Chemistry, Vol. 281, Issue 2
  • DOI: 10.1074/jbc.M511021200

T-Coffee: a web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension
journal, May 2011

  • Di Tommaso, P.; Moretti, S.; Xenarios, I.
  • Nucleic Acids Research, Vol. 39, Issue suppl
  • DOI: 10.1093/nar/gkr245

Functional Characterization of Flagellin Glycosylation in Campylobacter jejuni 81-176
journal, September 2009

  • Ewing, C. P.; Andreishcheva, E.; Guerry, P.
  • Journal of Bacteriology, Vol. 191, Issue 22
  • DOI: 10.1128/JB.00378-09

The Dual-Functioning Fumarate Reductase Is the Sole Succinate:Quinone Reductase in Campylobacter jejuni and Is Required for Full Host Colonization
journal, June 2009

  • Weingarten, R. A.; Taveirne, M. E.; Olson, J. W.
  • Journal of Bacteriology, Vol. 191, Issue 16
  • DOI: 10.1128/JB.00166-09

Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages
journal, July 2006

  • Coward, C.; Grant, A. J.; Swift, C.
  • Applied and Environmental Microbiology, Vol. 72, Issue 7
  • DOI: 10.1128/AEM.00184-06

Targeted Metabolomics Analysis of Campylobacter coli VC167 Reveals Legionaminic Acid Derivatives as Novel Flagellar Glycans
journal, March 2007

  • McNally, David J.; Aubry, Annie J.; Hui, Joseph P. M.
  • Journal of Biological Chemistry, Vol. 282, Issue 19
  • DOI: 10.1074/jbc.M611027200

The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
journal, February 2000

  • Parkhill, J.; Wren, B. W.; Mungall, K.
  • Nature, Vol. 403, Issue 6770
  • DOI: 10.1038/35001088

Phenotypic Screening of a Targeted Mutant Library Reveals Campylobacter jejuni Defenses against Oxidative Stress
journal, March 2014

  • Flint, A.; Sun, Y. -Q.; Butcher, J.
  • Infection and Immunity, Vol. 82, Issue 6
  • DOI: 10.1128/IAI.01528-13

Dynamics of mechanosensing in the bacterial flagellar motor
journal, July 2013

  • Lele, P. P.; Hosu, B. G.; Berg, H. C.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 29
  • DOI: 10.1073/pnas.1305885110

Campylobacter jejuni Glycosylation Island Important in Cell Charge, Legionaminic Acid Biosynthesis, and Colonization of Chickens
journal, March 2009

  • Howard, S. L.; Jagannathan, A.; Soo, E. C.
  • Infection and Immunity, Vol. 77, Issue 6
  • DOI: 10.1128/IAI.01425-08

Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014


Campylobacter – a tale of two protein glycosylation systems
journal, May 2003


Re-annotation and re-analysis of the Campylobacter jejuni NCTC11168 genome sequence
journal, January 2007