Optical coherence tomography imaging of plant root growth in soil

Larimer, Curtis J.; Denis, Elizabeth H.; Suter, Jonathan D.; Moran, James J.

doi:10.1364/AO.384674

Title: Optical coherence tomography imaging of plant root growth in soil

Abstract

Complex interactions between roots and soil provide the nutrients and physical support required for robust plant growth. Yet, visualizing the root-soil interface is challenged by soil’s opaque scattering characteristics. In this paper, we describe methods for using optical coherence tomography (OCT) to provide non-destructive 3D and cross-sectional root imaging not available with traditional bright field microscopy. OCT is regularly used for bioimaging, especially in ophthalmology where it can detect retinal abnormalities. Prior use of OCT in plant biology has focused on surface defects of above ground tissues, predominantly in food crops. Our results show OCT is also viable for detailed, in situ study of living plant roots.

Authors:

^[1]; Denis, Elizabeth H. ^[1]; Suter, Jonathan D. ^[1];

^[1]

Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Publication Date:: Thu Feb 13 00:00:00 EST 2020

Research Org.:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Great Lakes Bioenergy Research Center; USDOE Laboratory Directed Research and Development (LDRD) Program

OSTI Identifier:: 1605095

Alternate Identifier(s):: OSTI ID: 1602346

Report Number(s):: PNNL-SA-149582
Journal ID: ISSN 1559-128X; APOPAI

Grant/Contract Number:: AC05-76RL01830; SC0018409; FC02-07ER64494

Resource Type:: Accepted Manuscript

Journal Name:: Applied Optics

Additional Journal Information:: Journal Volume: 59; Journal Issue: 8; Journal ID: ISSN 1559-128X

Publisher:: Optical Society of America

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; optical coherence tomography, switchgrass, imaging plant roots

Citation Formats


                    Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., and Moran, James J. Optical coherence tomography imaging of plant root growth in soil.  United States: N. p., 2020. 
Web.  doi:10.1364/AO.384674.

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                    Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., & Moran, James J. Optical coherence tomography imaging of plant root growth in soil.  United States.  https://doi.org/10.1364/AO.384674

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                    Larimer, Curtis J., Denis, Elizabeth H., Suter, Jonathan D., and Moran, James J. Thu .  
"Optical coherence tomography imaging of plant root growth in soil".  United States.  https://doi.org/10.1364/AO.384674.  https://www.osti.gov/servlets/purl/1605095.

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@article{osti_1605095,

  title        = {Optical coherence tomography imaging of plant root growth in soil},

  author       = {Larimer, Curtis J. and Denis, Elizabeth H. and Suter, Jonathan D. and Moran, James J.},

  abstractNote = {Complex interactions between roots and soil provide the nutrients and physical support required for robust plant growth. Yet, visualizing the root-soil interface is challenged by soil’s opaque scattering characteristics. In this paper, we describe methods for using optical coherence tomography (OCT) to provide non-destructive 3D and cross-sectional root imaging not available with traditional bright field microscopy. OCT is regularly used for bioimaging, especially in ophthalmology where it can detect retinal abnormalities. Prior use of OCT in plant biology has focused on surface defects of above ground tissues, predominantly in food crops. Our results show OCT is also viable for detailed, in situ study of living plant roots.},

  doi          = {10.1364/AO.384674},

  journal      = {Applied Optics},

  number       = 8,

  volume       = 59,

  place        = {United States},

  year         = {Thu Feb 13 00:00:00 EST 2020},

  month        = {Thu Feb 13 00:00:00 EST 2020}

}

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https://doi.org/10.1364/AO.384674

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