Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates

Zaramela, Livia S.; Martino, Cameron; Alisson-Silva, Frederico; Rees, Steven D.; Diaz, Sandra L.; Chuzel, Léa; Ganatra, Mehul B.; Taron, Christopher H.; Secrest, Patrick; Zuñiga, Cristal; Huang, Jianbo; Siegel, Dionicio; Chang, Geoffrey; Varki, Ajit; Zengler, Karsten

doi:10.1038/s41564-019-0564-9

Title: Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates

Journal Article · 23 September 2019 · Nature Microbiology

DOI: https://doi.org/10.1038/s41564-019-0564-9 · OSTI ID:1600797

^[1];

^[1]; Diaz, Sandra L. ^[1]; Chuzel, Léa ^[2]; Ganatra, Mehul B. ^[2]; Taron, Christopher H. ^[2]; Secrest, Patrick ^[1];

^[1]; Huang, Jianbo ^[1]; Siegel, Dionicio ^[1]; Chang, Geoffrey ^[1];

^[1];

^[1]

Univ. of California, San Diego, CA (United States)
New England Biolabs, Ipswich, MA (United States)

Dietary habits have been associated with alterations of the human gut resident microorganisms contributing to obesity, diabetes and cancer. In Western diets, red meat is a frequently eaten food, but long-term consumption has been associated with increased risk of disease. Red meat is enriched in N-glycolylneuraminic acid (Neu5Gc) that cannot be synthesized by humans. However, consumption can cause Neu5Gc incorporation into cell surface glycans, especially in carcinomas. As a consequence, an inflammatory response is triggered when Neu5Gc-containing glycans encounter circulating anti-Neu5Gc antibodies. Although bacteria can use free sialic acids as a nutrient source, it is currently unknown if gut microorganisms contribute to releasing Neu5Gc from food. Iin this work, we found that a Neu5Gc-rich diet induces changes in the gut microbiota, with Bacteroidales and Clostridiales responding the most. Genome assembling of mouse and human shotgun metagenomic sequencing identified bacterial sialidases with previously unobserved substrate preference for Neu5Gc-containing glycans. X-ray crystallography revealed key amino acids potentially contributing to substrate preference. Additionally, we verified that mouse and human sialidases were able to release Neu5Gc from red meat. The release of Neu5Gc from red meat using bacterial sialidases could reduce the risk of inflammatory diseases associated with red meat consumption, including colorectal cancer and atherosclerosis.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF); Program Science Without Borders Bex

Grant/Contract Number:: R01GM32373; IOS-1444435; T32GM8806

OSTI ID:: 1600797

Journal Information:: Nature Microbiology, Vol. 4, Issue 12; ISSN 2058-5276

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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Cited by: 44 works

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Search and clustering orders of magnitude faster than BLAST Edgar, Robert C. Bioinformatics, Vol. 26, Issue 19, p. 2460-2461 https://doi.org/10.1093/bioinformatics/btq461	journal	August 2010
dbCAN-seq: a database of carbohydrate-active enzyme (CAZyme) sequence and annotation Huang, Le; Zhang, Han; Wu, Peizhi Nucleic Acids Research, Vol. 46, Issue D1 https://doi.org/10.1093/nar/gkx894	journal	October 2017
metaSPAdes: a new versatile metagenomic assembler Nurk, Sergey; Meleshko, Dmitry; Korobeynikov, Anton Genome Research, Vol. 27, Issue 5 https://doi.org/10.1101/gr.213959.116	journal	March 2017
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Title: Gut bacteria responding to dietary change encode sialidases that exhibit preference for red meat-associated carbohydrates

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