PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1 Δ3trans FATTY ACID DESATURASE4
Abstract
Summary Thylakoid membrane lipids, comprised of glycolipids and the phospholipid phosphatidylglycerol (PG), are essential for normal plant growth and development. Unlike other lipid classes, chloroplast PG in nearly all plants contains a substantial fraction of the unusual trans fatty acid 16:1 Δ3trans or 16:1t. We determined that, in Arabidopsis thaliana , 16:1t biosynthesis requires both FATTY ACID DESATURASE4 (FAD4) and a thylakoid‐associated redox protein, PEROXIREDOXIN Q (PRXQ), to produce wild‐type levels of 16:1t. The FAD4–PRXQ biochemical relationship appears to be very specific in planta , as other fatty acids (FA) desaturases do not require peroxiredoxins for their activity, nor does FAD4 require other chloroplast peroxiredoxins under standard growth conditions. Although most of chloroplast PG assembly occurs at the inner envelope membrane, FAD4 was primarily associated with the thylakoid membranes facing the stroma. Furthermore, co‐production of PRXQ with FAD4 was required to produce Δ3‐desaturated FAs in yeast. Alteration of the redox state of FAD4 or PRXQ through site‐directed mutagenesis of conserved cysteine residues impaired Δ3 FA production. However, these mutations did not appear to directly alter disulfide status of FAD4. These results collectively demonstrate that the production of 16:1t is linked to the redox status of the chloroplast through PRXQ associatedmore »
- Authors:
-
[1];
[4]
- Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA
- Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA, Department of Chemical Engineering and Materials Science Michigan State University East Lansing MI 48824 USA
- Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI 48824 USA
- Department of Energy‐Plant Research Laboratory Michigan State University East Lansing MI 48824 USA, Department of Biochemistry and Molecular Biology Michigan State University East Lansing MI 48824 USA, Department of Plant Biology Michigan State University East Lansing MI 48824 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1596397
- Grant/Contract Number:
- DE‐FG02‐91ER20021; DE‐FG02‐98ER20305
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 102 Journal Issue: 4; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Horn, Patrick J., Smith, Montgomery D., Clark, Tessa R., Froehlich, John E., and Benning, Christoph. PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1 Δ3trans FATTY ACID DESATURASE4. United Kingdom: N. p., 2020.
Web. doi:10.1111/tpj.14657.
Horn, Patrick J., Smith, Montgomery D., Clark, Tessa R., Froehlich, John E., & Benning, Christoph. PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1 Δ3trans FATTY ACID DESATURASE4. United Kingdom. https://doi.org/10.1111/tpj.14657
Horn, Patrick J., Smith, Montgomery D., Clark, Tessa R., Froehlich, John E., and Benning, Christoph. Fri .
"PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1 Δ3trans FATTY ACID DESATURASE4". United Kingdom. https://doi.org/10.1111/tpj.14657.
@article{osti_1596397,
title = {PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1 Δ3trans FATTY ACID DESATURASE4},
author = {Horn, Patrick J. and Smith, Montgomery D. and Clark, Tessa R. and Froehlich, John E. and Benning, Christoph},
abstractNote = {Summary Thylakoid membrane lipids, comprised of glycolipids and the phospholipid phosphatidylglycerol (PG), are essential for normal plant growth and development. Unlike other lipid classes, chloroplast PG in nearly all plants contains a substantial fraction of the unusual trans fatty acid 16:1 Δ3trans or 16:1t. We determined that, in Arabidopsis thaliana , 16:1t biosynthesis requires both FATTY ACID DESATURASE4 (FAD4) and a thylakoid‐associated redox protein, PEROXIREDOXIN Q (PRXQ), to produce wild‐type levels of 16:1t. The FAD4–PRXQ biochemical relationship appears to be very specific in planta , as other fatty acids (FA) desaturases do not require peroxiredoxins for their activity, nor does FAD4 require other chloroplast peroxiredoxins under standard growth conditions. Although most of chloroplast PG assembly occurs at the inner envelope membrane, FAD4 was primarily associated with the thylakoid membranes facing the stroma. Furthermore, co‐production of PRXQ with FAD4 was required to produce Δ3‐desaturated FAs in yeast. Alteration of the redox state of FAD4 or PRXQ through site‐directed mutagenesis of conserved cysteine residues impaired Δ3 FA production. However, these mutations did not appear to directly alter disulfide status of FAD4. These results collectively demonstrate that the production of 16:1t is linked to the redox status of the chloroplast through PRXQ associated with the thylakoids.},
doi = {10.1111/tpj.14657},
journal = {The Plant Journal},
number = 4,
volume = 102,
place = {United Kingdom},
year = {Fri Jan 31 00:00:00 EST 2020},
month = {Fri Jan 31 00:00:00 EST 2020}
}
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