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Title: Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)

Abstract

Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in swine. Using the yeast two-hybrid system, we identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We further confirmed the occurrence of this interaction in CSFV-infected swine cells by using two independent methodologies: Co-immunoprecipitation and Proximity Ligation Assay. In addition, we demonstrated that pharmacological activation of the PP1 pathway has a negative effect on CSFV replication while inhibition of the PP1 pathway or knockdown of PPP1CB by siRNA had no observed effect. Overall, our data suggests that the CSFV E2 and PPP1CB protein interact in infected cells, and that activation of the PP1 pathway decreases virus replication.

Authors:
 [1];  [2];  [3];  [4];  [3]; ORCiD logo [3]
+ Show Author Affiliations
  1. USDA, Greenport, NY (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
  2. USDA, Greenport, NY (United States); Univ. of Connecticut, Storrs, CT (United States)
  3. USDA, Greenport, NY (United States)
  4. USDA, Greenport, NY (United States); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States); Johns Hopkins Applied Physics Lab., Laurel, MD (United States)
Publication Date:
Research Org.:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1580724
Grant/Contract Number:  
SC0014664
Resource Type:
Accepted Manuscript
Journal Name:
Viruses
Additional Journal Information:
Journal Volume: 11; Journal Issue: 4; Journal ID: ISSN 1999-4915
Publisher:
MDPI
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; E2; classical swine fever virus; CSFV; Viral-Host protein interactions

Citation Formats

Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Holinka, Lauren G., Baker-Branstetter, Ryan, Borca, Manuel V., and Gladue, Douglas P. Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB). United States: N. p., 2019. Web. doi:10.3390/v11040307.
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Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Holinka, Lauren G., Baker-Branstetter, Ryan, Borca, Manuel V., & Gladue, Douglas P. Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB). United States. https://doi.org/10.3390/v11040307
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Vuono, Elizabeth A., Ramirez-Medina, Elizabeth, Holinka, Lauren G., Baker-Branstetter, Ryan, Borca, Manuel V., and Gladue, Douglas P. Fri . "Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)". United States. https://doi.org/10.3390/v11040307. https://www.osti.gov/servlets/purl/1580724.
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@article{osti_1580724,
title = {Interaction of Structural Glycoprotein E2 of Classical Swine Fever Virus with Protein Phosphatase 1 Catalytic Subunit Beta (PPP1CB)},
author = {Vuono, Elizabeth A. and Ramirez-Medina, Elizabeth and Holinka, Lauren G. and Baker-Branstetter, Ryan and Borca, Manuel V. and Gladue, Douglas P.},
abstractNote = {Classical swine fever virus (CSFV) E2 protein, the major virus structural glycoprotein, is an essential component of the viral envelope. E2 is involved in virus absorption, induction of a protective immune response and is critical for virulence in swine. Using the yeast two-hybrid system, we identified protein phosphatase 1 catalytic subunit beta (PPP1CB), which is part of the Protein Phosphatase 1 (PP1) complex, as a specific binding host partner for E2. We further confirmed the occurrence of this interaction in CSFV-infected swine cells by using two independent methodologies: Co-immunoprecipitation and Proximity Ligation Assay. In addition, we demonstrated that pharmacological activation of the PP1 pathway has a negative effect on CSFV replication while inhibition of the PP1 pathway or knockdown of PPP1CB by siRNA had no observed effect. Overall, our data suggests that the CSFV E2 and PPP1CB protein interact in infected cells, and that activation of the PP1 pathway decreases virus replication.},
doi = {10.3390/v11040307},
journal = {Viruses},
number = 4,
volume = 11,
place = {United States},
year = {Fri Mar 29 00:00:00 EDT 2019},
month = {Fri Mar 29 00:00:00 EDT 2019}
}
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https://doi.org/10.3390/v11040307
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    Works referencing / citing this record:

    Structural Glycoprotein E2 of Classical Swine Fever Virus Interacts with Host Protein Dynactin Subunit 6 (DCTN6) during the Virus Infectious Cycle
    journal, October 2019

    • Borca, M. V.; Vuono, E. A.; Ramirez-Medina, E.
    • Journal of Virology, Vol. 94, Issue 1
    • DOI: 10.1128/jvi.01642-19
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