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Title: Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development

Abstract

The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families. Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues. Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems. High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize ( Zea mays, cultivar B73). Of 1239 of these genes, 854 were expressed among the internodes at =95 reads per 20 M, and 693 of them at =500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis. Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development. For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions. The same members of gene families were often expressed in two maize inbreds, B73 and Mo17,more » but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage. Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements. Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another. Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.« less

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [5]; ORCiD logo [6]; ORCiD logo [7]; ORCiD logo [8]; ORCiD logo [9]; ORCiD logo [10]
+ Show Author Affiliations
  1. Purdue Univ., West Lafayette, IN (United States). Dept. of Botany & Plant Pathology; Purdue Univ., West Lafayette, IN (United States). Dept. of Biological Sciences; USDA-ARS, Wooster, OH (United States). Wheat Quality Research Unit
  2. Purdue Univ., West Lafayette, IN (United States). Dept. of Botany & Plant Pathology; Dept. de Alimentos e Nutrição Experimental, Sao Paulo (Brazil)
  3. Purdue Univ., West Lafayette, IN (United States). Dept. of Botany & Plant Pathology
  4. Purdue Univ., West Lafayette, IN (United States). Genomics Core Facility
  5. Purdue Univ., West Lafayette, IN (United States). Bioinformatics Core Facility; Cleveland Clinic, Cleveland, OH (United States). Dept. of Internal Medicine
  6. Dept. de Alimentos e Nutrição Experimental, Sao Paulo (Brazil); Purdue Univ., West Lafayette, IN (United States). Bioinformatics Core Facility
  7. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center; Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
  8. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
  9. Purdue Univ., West Lafayette, IN (United States). Dept. of Biological Sciences; Plant Center for Plant Biology, West Lafayette, IN (United States)
  10. Purdue Univ., West Lafayette, IN (United States). Dept. of Botany & Plant Pathology; Purdue Univ., West Lafayette, IN (United States). Dept. of Biological Sciences; Purdue Center for Plant Biology, West Lafayette, IN (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1573961
Report Number(s):
NREL/JA-5100-74676
Journal ID: ISSN 1471-2164
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
BMC Genomics
Additional Journal Information:
Journal Volume: 20; Journal Issue: 1; Journal ID: ISSN 1471-2164
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Zea mays; maize; stem development; cell-wall biosynthesis; gene expression; transcript profiling; lignocellulosic biomass

Citation Formats

Penning, Bryan W., Shiga, Tânia M., Klimek, John F., SanMiguel, Philip J., Shreve, Jacob, Thimmapuram, Jyothi, Sykes, Robert W., Davis, Mark F., McCann, Maureen C., and Carpita, Nicholas C. Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development. United States: N. p., 2019. Web. doi:10.1186/s12864-019-6117-z.
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Penning, Bryan W., Shiga, Tânia M., Klimek, John F., SanMiguel, Philip J., Shreve, Jacob, Thimmapuram, Jyothi, Sykes, Robert W., Davis, Mark F., McCann, Maureen C., & Carpita, Nicholas C. Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development. United States. doi:10.1186/s12864-019-6117-z.
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Penning, Bryan W., Shiga, Tânia M., Klimek, John F., SanMiguel, Philip J., Shreve, Jacob, Thimmapuram, Jyothi, Sykes, Robert W., Davis, Mark F., McCann, Maureen C., and Carpita, Nicholas C. Tue . "Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development". United States. doi:10.1186/s12864-019-6117-z. https://www.osti.gov/servlets/purl/1573961.
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@article{osti_1573961,
title = {Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development},
author = {Penning, Bryan W. and Shiga, Tânia M. and Klimek, John F. and SanMiguel, Philip J. and Shreve, Jacob and Thimmapuram, Jyothi and Sykes, Robert W. and Davis, Mark F. and McCann, Maureen C. and Carpita, Nicholas C.},
abstractNote = {The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families. Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues. Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems. High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73). Of 1239 of these genes, 854 were expressed among the internodes at =95 reads per 20 M, and 693 of them at =500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis. Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development. For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions. The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage. Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements. Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another. Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.},
doi = {10.1186/s12864-019-6117-z},
journal = {BMC Genomics},
number = 1,
volume = 20,
place = {United States},
year = {2019},
month = {10}
}
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Works referenced in this record:

Biomass recalcitrance: a multi-scale, multi-factor, and conversion-specific property: Fig. 1.
journal, June 2015

  • McCann, Maureen C.; Carpita, Nicholas C.
  • Journal of Experimental Botany, Vol. 66, Issue 14
  • DOI: 10.1093/jxb/erv267

Identification of Novel Genes in Arabidopsis Involved in Secondary Cell Wall Formation Using Expression Profiling and Reverse Genetics
journal, June 2005

  • Brown, David M.; Zeef, Leo A. H.; Ellis, Joanne
  • The Plant Cell, Vol. 17, Issue 8
  • DOI: 10.1105/tpc.105.031542

Genome-wide transcriptome analysis of the transition from primary to secondary stem development in Populus trichocarpa
journal, January 2010

  • Dharmawardhana, Palitha; Brunner, Amy M.; Strauss, Steven H.
  • BMC Genomics, Vol. 11, Issue 1
  • DOI: 10.1186/1471-2164-11-150

Comparative interrogation of the developing xylem transcriptomes of two wood-forming species: Populus trichocarpa and Eucalyptus grandis
journal, February 2015

  • Hefer, Charles A.; Mizrachi, Eshchar; Myburg, Alexander A.
  • New Phytologist, Vol. 206, Issue 4
  • DOI: 10.1111/nph.13277

Dynamics of biomass partitioning, stem gene expression, cell wall biosynthesis, and sucrose accumulation during development of Sorghum bicolor
journal, September 2016

  • McKinley, Brian; Rooney, William; Wilkerson, Curtis
  • The Plant Journal, Vol. 88, Issue 4
  • DOI: 10.1111/tpj.13269

The Sorghum bicolor reference genome: improved assembly, gene annotations, a transcriptome atlas, and signatures of genome organization
journal, December 2017

  • McCormick, Ryan F.; Truong, Sandra K.; Sreedasyam, Avinash
  • The Plant Journal, Vol. 93, Issue 2
  • DOI: 10.1111/tpj.13781

Genomics of plant cell wall biogenesis
journal, June 2005

Genetic Resources for Maize Cell Wall Biology
journal, November 2009

  • Penning, Bryan W.; Hunter, Charles T.; Tayengwa, Reuben
  • Plant Physiology, Vol. 151, Issue 4
  • DOI: 10.1104/pp.109.136804

Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.)
journal, July 2001

  • Tenaillon, M. I.; Sawkins, M. C.; Long, A. D.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 16
  • DOI: 10.1073/pnas.151244298

Maize Inbreds Exhibit High Levels of Copy Number Variation (CNV) and Presence/Absence Variation (PAV) in Genome Content
journal, November 2009

Genetic Determinants for Enzymatic Digestion of Lignocellulosic Biomass Are Independent of Those for Lignin Abundance in a Maize Recombinant Inbred Population
journal, June 2014

  • Penning, Bryan W.; Sykes, Robert W.; Babcock, Nicholas C.
  • Plant Physiology, Vol. 165, Issue 4
  • DOI: 10.1104/pp.114.242446

Complementation contributes to transcriptome complexity in maize (Zea mays L.) hybrids relative to their inbred parents
journal, October 2012

Genome-wide atlas of transcription during maize development
journal, March 2011

Maize Brittle stalk2 Encodes a COBRA-Like Protein Expressed in Early Organ Development But Required for Tissue Flexibility at Maturity
journal, October 2007

  • Sindhu, Anoop; Langewisch, Tiffany; Olek, Anna
  • Plant Physiology, Vol. 145, Issue 4
  • DOI: 10.1104/pp.107.102582

Endo-(1,4)-β-Glucanase gene families in the grasses: temporal and spatial Co-transcription of orthologous genes1
journal, January 2012

  • Buchanan, Margaret; Burton, Rachel A.; Dhugga, Kanwarpal S.
  • BMC Plant Biology, Vol. 12, Issue 1
  • DOI: 10.1186/1471-2229-12-235

Characterization of IRX10 and IRX10-like reveals an essential role in glucuronoxylan biosynthesis in Arabidopsis
journal, February 2009

O-Acetylation of Plant Cell Wall Polysaccharides
journal, January 2012

Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice
journal, November 2016

  • Gao, Yaping; He, Congwu; Zhang, Dongmei
  • Plant Physiology, Vol. 173, Issue 1
  • DOI: 10.1104/pp.16.01618

Grass cell wall feruloylation: distribution of bound ferulate and candidate gene expression in Brachypodium distachyon
journal, January 2013

  • Molinari, Hugo B. C.; Pellny, Till K.; Freeman, Jackie
  • Frontiers in Plant Science, Vol. 4
  • DOI: 10.3389/fpls.2013.00050

Arabidopsis Deficient in Cutin Ferulate Encodes a Transferase Required for Feruloylation of ω-Hydroxy Fatty Acids in Cutin Polyester
journal, December 2011

  • Rautengarten, Carsten; Ebert, Berit; Ouellet, Mario
  • Plant Physiology, Vol. 158, Issue 2
  • DOI: 10.1104/pp.111.187187

The polysaccharide composition of Poales cell walls
journal, January 1999

Cell Wall Architecture of the Elongating Maize Coleoptile
journal, October 2001

  • Carpita, Nicholas C.; Defernez, Marianne; Findlay, Kim
  • Plant Physiology, Vol. 127, Issue 2
  • DOI: 10.1104/pp.010146

Neural Network Analyses of Infrared Spectra for Classifying Cell Wall Architectures
journal, January 2007

  • McCann, Maureen C.; Defernez, Marianne; Urbanowicz, Breeanna R.
  • Plant Physiology, Vol. 143, Issue 3
  • DOI: 10.1104/pp.106.093054

Abundance of mixed linkage glucan in mature tissues and secondary cell walls of grasses
journal, February 2013

  • Vega-Sanchez, Miguel; Verhertbruggen, Yves; Scheller, Henrik Vibe
  • Plant Signaling & Behavior, Vol. 8, Issue 2
  • DOI: 10.4161/psb.23143

Pectin RG-I rhamnosyltransferases represent a novel plant-specific glycosyltransferase family
journal, August 2018

Identification of an additional protein involved in mannan biosynthesis
journal, October 2012

  • Wang, Yan; Mortimer, Jennifer C.; Davis, Jonathan
  • The Plant Journal, Vol. 73, Issue 1
  • DOI: 10.1111/tpj.12019

A DUF-246 family glycosyltransferase-like gene affects male fertility and the biosynthesis of pectic arabinogalactans
journal, April 2016

Loosening of plant cell walls by expansins
journal, September 2000

The XTH Family of Enzymes Involved in Xyloglucan Endotransglucosylation and Endohydrolysis: Current Perspectives and a New Unifying Nomenclature
journal, December 2002

  • Rose, Jocelyn K. C.; Braam, Janet; Fry, Stephen C.
  • Plant and Cell Physiology, Vol. 43, Issue 12
  • DOI: 10.1093/pcp/pcf171

STAMP: a web tool for exploring DNA-binding motif similarities
journal, May 2007

  • Mahony, S.; Benos, P. V.
  • Nucleic Acids Research, Vol. 35, Issue Web Server
  • DOI: 10.1093/nar/gkm272

PlantPAN3.0: a new and updated resource for reconstructing transcriptional regulatory networks from ChIP-seq experiments in plants
journal, November 2018

  • Chow, Chi-Nga; Lee, Tzong-Yi; Hung, Yu-Cheng
  • Nucleic Acids Research, Vol. 47, Issue D1
  • DOI: 10.1093/nar/gky1081

Characterisation of a pine MYB that regulates lignification
journal, December 2003

Characterisation of PtMYB1, an R2R3-MYB from pine xylem
journal, November 2003

A cis-element containing PAL-box functions in the expression of the wound-inducible peroxidase gene of horseradish
journal, May 2000

  • Kaothien, P.; Shimokawatoko, Y.; Kawaoka, A.
  • Plant Cell Reports, Vol. 19, Issue 6
  • DOI: 10.1007/s002990050773

Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis
journal, May 2000

The orientation of transcription factor binding site motifs in gene promoter regions: does it matter?
journal, March 2016

Structure, organization and evolution of ADP-ribosylation factors in rice and foxtail millet and their expression in rice
journal, April 2016

  • Muthamilarasan, Mehanathan; Mangu, Venkata R.; Zandkarimi, Hana
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep24008

Identification of Gene Modules Associated with Drought Response in Rice by Network-Based Analysis
journal, May 2012

Functional characterization of drought-responsive modules and genes in Oryza sativa: a network-based approach
journal, July 2015

Regulation of plant biomass production
journal, June 2010

Evolutionary conservation of the transcriptional network regulating secondary cell wall biosynthesis
journal, November 2010

Transcriptional networks for lignin biosynthesis: more complex than we thought?
journal, April 2011

Genetic variation and breeding strategies for improved cell wall digestibility in annual forage crops. A review
journal, May 2003

  • Barrière, Yves; Guillet, Carine; Goffner, Deborah
  • Animal Research, Vol. 52, Issue 3
  • DOI: 10.1051/animres:2003018

Modifying crops to increase cell wall digestibility
journal, April 2012

Improvement of biomass through lignin modification
journal, May 2008

Enhancing cellulose utilization for fuels and chemicals by genetic modification of plant cell wall architecture
journal, April 2015

Sugar release and growth of biofuel crops are improved by downregulation of pectin biosynthesis
journal, February 2018

  • Biswal, Ajaya K.; Atmodjo, Melani A.; Li, Mi
  • Nature Biotechnology, Vol. 36, Issue 3
  • DOI: 10.1038/nbt.4067

Three Distinct Rice Cellulose Synthase Catalytic Subunit Genes Required for Cellulose Synthesis in the Secondary Wall
journal, August 2003

  • Tanaka, Katsuyuki; Murata, Kazumasa; Yamazaki, Muneo
  • Plant Physiology, Vol. 133, Issue 1
  • DOI: 10.1104/pp.103.022442

Cellulose synthesis in maize: isolation and expression analysis of the cellulose synthase (CesA) gene family
journal, September 2004

Cellulose biosynthesis and deposition in higher plants
journal, April 2008

Catalytic Subunit Stoichiometry within the Cellulose Synthase Complex
journal, October 2014

  • Gonneau, Martine; Desprez, Thierry; Guillot, Alain
  • Plant Physiology, Vol. 166, Issue 4
  • DOI: 10.1104/pp.114.250159

The Arabidopsis Cellulose Synthase Complex: A Proposed Hexamer of CESA Trimers in an Equimolar Stoichiometry
journal, December 2014

  • Hill, Joseph L.; Hammudi, Mustafa B.; Tien, Ming
  • The Plant Cell, Vol. 26, Issue 12
  • DOI: 10.1105/tpc.114.131193

Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce
journal, May 2018

  • Zhang, Xueyang; Dominguez, Pia Guadalupe; Kumar, Manoj
  • Plant Physiology, Vol. 177, Issue 3
  • DOI: 10.1104/pp.18.00394

Glycome and Proteome Components of Golgi Membranes Are Common between Two Angiosperms with Distinct Cell-Wall Structures
journal, March 2019

  • Okekeogbu, Ikenna O.; Pattathil, Sivakumar; González Fernández-Niño, Susana M.
  • The Plant Cell, Vol. 31, Issue 5
  • DOI: 10.1105/tpc.18.00755

Arabidopsis irregular xylem8 and irregular xylem9 : Implications for the Complexity of Glucuronoxylan Biosynthesis
journal, February 2007

Comparison of five xylan synthesis mutants reveals new insight into the mechanisms of xylan synthesis
journal, December 2007

The Arabidopsis IRX10 and IRX10-LIKE glycosyltransferases are critical for glucuronoxylan biosynthesis during secondary cell wall formation
journal, February 2009

Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses
journal, January 2012

  • Anders, N.; Wilkinson, M. D.; Lovegrove, A.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 3
  • DOI: 10.1073/pnas.1115858109

XAX1 from glycosyltransferase family 61 mediates xylosyltransfer to rice xylan
journal, October 2012

  • Chiniquy, D.; Sharma, V.; Schultink, A.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 42
  • DOI: 10.1073/pnas.1202079109

Remarkable variation in maize genome structure inferred from haplotype diversity at the bz locus
journal, November 2006

  • Wang, Q.; Dooner, H. K.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 47
  • DOI: 10.1073/pnas.0603080103

Pervasive gene content variation and copy number variation in maize and its undomesticated progenitor
journal, October 2010

  • Swanson-Wagner, R. A.; Eichten, S. R.; Kumari, S.
  • Genome Research, Vol. 20, Issue 12
  • DOI: 10.1101/gr.109165.110

Nonsyntenic Genes Drive Highly Dynamic Complementation of Gene Expression in Maize Hybrids
journal, October 2014

  • Paschold, Anja; Larson, Nick B.; Marcon, Caroline
  • The Plant Cell, Vol. 26, Issue 10
  • DOI: 10.1105/tpc.114.130948

Identification and characterization of presence/absence variation in maize genotype Mo17
journal, April 2015

Gene duplication and exon shuffling by helitron-like transposons generate intraspecies diversity in maize
journal, July 2005

  • Morgante, Michele; Brunner, Stephan; Pea, Giorgio
  • Nature Genetics, Vol. 37, Issue 9
  • DOI: 10.1038/ng1615

High-resolution genetic mapping of maize pan-genome sequence anchors
journal, April 2015

  • Lu, Fei; Romay, Maria C.; Glaubitz, Jeffrey C.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms7914

Comprehensive genotyping of the USA national maize inbred seed bank
journal, June 2013

  • Romay, Maria C.; Millard, Mark J.; Glaubitz, Jeffrey C.
  • Genome Biology, Vol. 14, Issue 6
  • DOI: 10.1186/gb-2013-14-6-r55

Genome-wide association study of leaf architecture in the maize nested association mapping population
journal, January 2011

  • Tian, Feng; Bradbury, Peter J.; Brown, Patrick J.
  • Nature Genetics, Vol. 43, Issue 2
  • DOI: 10.1038/ng.746

The Genetic Architecture of Maize Flowering Time
journal, August 2009

  • Buckler, E. S.; Holland, J. B.; Bradbury, P. J.
  • Science, Vol. 325, Issue 5941, p. 714-718
  • DOI: 10.1126/science.1174276

The Genetic Architecture Of Maize Height
journal, February 2014

Validation of PyMBMS as a High-throughput Screen for Lignin Abundance in Lignocellulosic Biomass of Grasses
journal, January 2014

  • Penning, Bryan W.; Sykes, Robert W.; Babcock, Nicholas C.
  • BioEnergy Research, Vol. 7, Issue 3
  • DOI: 10.1007/s12155-014-9410-3

Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
journal, January 2009

The developmental dynamics of the maize leaf transcriptome
journal, October 2010

  • Li, Pinghua; Ponnala, Lalit; Gandotra, Neeru
  • Nature Genetics, Vol. 42, Issue 12, p. 1060-1067
  • DOI: 10.1038/ng.703

Characterizing Regulatory and Functional Differentiation between Maize Mesophyll and Bundle Sheath Cells by Transcriptomic Analysis
journal, July 2012

  • Chang, Yao-Ming; Liu, Wen-Yu; Shih, Arthur Chun-Chieh
  • Plant Physiology, Vol. 160, Issue 1
  • DOI: 10.1104/pp.112.203810

Genesis: cluster analysis of microarray data
journal, January 2002

edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009

Multiple sequence alignment with the Clustal series of programs
journal, July 2003

TreeDyn: towards dynamic graphics and annotations for analyses of trees
journal, October 2006

  • Chevenet, François; Brun, Christine; Bañuls, Anne-Laure
  • BMC Bioinformatics, Vol. 7, Issue 1
  • DOI: 10.1186/1471-2105-7-439
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