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Title: Photobleaching statistics in single-molecule on-/off-time distributions

Abstract

The on- and and off-time distributions from fluorescence single-molecule experiments are widely used to extract kinetics parameters with the goal to provide a quantitative description for the molecule’s behavior on the ensemble level. Such experiments are inevitably influenced by photobleaching, where the fluorescent probe transitions to a nonemissive state. Yet, it appears that few reports went beyond acknowledging this unavoidable complication; in fact, it has so far been ignored when evaluating off-time distributions. Here in this paper, we present a theoretical framework that allows the derivation of analytical equations in which photobleaching kinetics are rigorously incorporated. Unexpectedly, our results indicate that the off-time distribution should be nonexponential even when all the rate processes are single exponential. With the analytical theory understood and demonstrated as easy to implement, such ubiquitous photochemical processes can now be readily included in routine experimental analyses.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
+ Show Author Affiliations
  1. Princeton Univ., NJ (United States)
Publication Date:
Research Org.:
Princeton Univ., NJ (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); Gordon and Betty Moore Foundation (GBMF)
OSTI Identifier:
1617128
Alternate Identifier(s):
OSTI ID: 1572738
Grant/Contract Number:  
SC0019364; 4741
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Chemical Physics
Additional Journal Information:
Journal Volume: 151; Journal Issue: 17; Journal ID: ISSN 0021-9606
Publisher:
American Institute of Physics (AIP)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Yang, Haw, Li, Hao, and Liu, Tao. Photobleaching statistics in single-molecule on-/off-time distributions. United States: N. p., 2019. Web. doi:10.1063/1.5126500.
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Yang, Haw, Li, Hao, & Liu, Tao. Photobleaching statistics in single-molecule on-/off-time distributions. United States. https://doi.org/10.1063/1.5126500
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Yang, Haw, Li, Hao, and Liu, Tao. Fri . "Photobleaching statistics in single-molecule on-/off-time distributions". United States. https://doi.org/10.1063/1.5126500. https://www.osti.gov/servlets/purl/1617128.
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@article{osti_1617128,
title = {Photobleaching statistics in single-molecule on-/off-time distributions},
author = {Yang, Haw and Li, Hao and Liu, Tao},
abstractNote = {The on- and and off-time distributions from fluorescence single-molecule experiments are widely used to extract kinetics parameters with the goal to provide a quantitative description for the molecule’s behavior on the ensemble level. Such experiments are inevitably influenced by photobleaching, where the fluorescent probe transitions to a nonemissive state. Yet, it appears that few reports went beyond acknowledging this unavoidable complication; in fact, it has so far been ignored when evaluating off-time distributions. Here in this paper, we present a theoretical framework that allows the derivation of analytical equations in which photobleaching kinetics are rigorously incorporated. Unexpectedly, our results indicate that the off-time distribution should be nonexponential even when all the rate processes are single exponential. With the analytical theory understood and demonstrated as easy to implement, such ubiquitous photochemical processes can now be readily included in routine experimental analyses.},
doi = {10.1063/1.5126500},
journal = {Journal of Chemical Physics},
number = 17,
volume = 151,
place = {United States},
year = {Fri Nov 01 00:00:00 EDT 2019},
month = {Fri Nov 01 00:00:00 EDT 2019}
}
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