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Title: Photobleaching statistics in single-molecule on-/off-time distributions

ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA
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Sponsoring Org.:
OSTI Identifier:
Grant/Contract Number:  
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Chemical Physics
Additional Journal Information:
Journal Name: Journal of Chemical Physics Journal Volume: 151 Journal Issue: 17; Journal ID: ISSN 0021-9606
American Institute of Physics
Country of Publication:
United States

Citation Formats

Yang, Haw, Li, Hao, and Liu, Tao. Photobleaching statistics in single-molecule on-/off-time distributions. United States: N. p., 2019. Web. doi:10.1063/1.5126500.
Yang, Haw, Li, Hao, & Liu, Tao. Photobleaching statistics in single-molecule on-/off-time distributions. United States. doi:10.1063/1.5126500.
Yang, Haw, Li, Hao, and Liu, Tao. Thu . "Photobleaching statistics in single-molecule on-/off-time distributions". United States. doi:10.1063/1.5126500.
title = {Photobleaching statistics in single-molecule on-/off-time distributions},
author = {Yang, Haw and Li, Hao and Liu, Tao},
abstractNote = {},
doi = {10.1063/1.5126500},
journal = {Journal of Chemical Physics},
number = 17,
volume = 151,
place = {United States},
year = {2019},
month = {11}

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Works referenced in this record:

Single-Molecule Dynamics of Enhanceosome Assembly in Embryonic Stem Cells
journal, March 2014

Single-molecule enzymology of RNA: Essential functional groups impact catalysis from a distance
journal, June 2004

  • Rueda, D.; Bokinsky, G.; Rhodes, M. M.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 27
  • DOI: 10.1073/pnas.0403575101

The photochemical oxidation of 4-arylazo-1-naphthols
journal, January 1972

  • Griffiths, J.; Hawkins, C.
  • Journal of the Chemical Society, Chemical Communications, Issue 8
  • DOI: 10.1039/c39720000463

Statistical Learning of Discrete States in Time Series
journal, December 2018

Green Fluorescent Protein Photobleaching: a Model for Protein Damage by Endogenous and Exogenous Singlet Oxygen
journal, January 2000

  • Greenbaum, Lior; Rothmann, Chana; Lavie, Ronit
  • Biological Chemistry, Vol. 381, Issue 12
  • DOI: 10.1515/bc.2000.153

A benchmark for chromatin binding measurements in live cells
journal, July 2012

  • Mazza, Davide; Abernathy, Alice; Golob, Nicole
  • Nucleic Acids Research, Vol. 40, Issue 15
  • DOI: 10.1093/nar/gks701

The Localization and Action of Topoisomerase IV in Escherichia coli Chromosome Segregation Is Coordinated by the SMC Complex, MukBEF
journal, December 2015

Single-molecule visualization of a formin-capping protein ‘decision complex’ at the actin filament barbed end
journal, November 2015

  • Bombardier, Jeffrey P.; Eskin, Julian A.; Jaiswal, Richa
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms9707

Hydrolysis of a Lipid Membrane by Single Enzyme Molecules: Accurate Determination of Kinetic Parameters
journal, November 2014

  • Rabe, Michael; Tabaei, Seyed R.; Zetterberg, Henrik
  • Angewandte Chemie International Edition, Vol. 54, Issue 3
  • DOI: 10.1002/anie.201409603

Kinetics of Complexin Binding to the SNARE Complex: Correcting Single Molecule FRET Measurements for Hidden Events
journal, September 2007

Parallelization of Change Point Detection
journal, June 2017

Reactions between the Triplet State of Fluorescein and Oxygen 1
journal, April 1964

  • Kasche, Volker; Lindqvist, Lars
  • The Journal of Physical Chemistry, Vol. 68, Issue 4
  • DOI: 10.1021/j100786a019

Influence of the triplet excited state on the photobleaching kinetics of fluorescein in microscopy
journal, June 1996

On/off blinking and switching behaviour of single molecules of green fluorescent protein
journal, July 1997

  • Dickson, Robert M.; Cubitt, Andrew B.; Tsien, Roger Y.
  • Nature, Vol. 388, Issue 6640
  • DOI: 10.1038/41048

Single-molecule imaging of DNA gyrase activity in living Escherichia coli
journal, November 2018

  • Stracy, Mathew; Wollman, Adam J. M.; Kaja, Elzbieta
  • Nucleic Acids Research, Vol. 47, Issue 1
  • DOI: 10.1093/nar/gky1143

Mapping the stochastic sequence of individual ligand-receptor binding events to cellular activation: T cells act on the rare events
journal, January 2019

  • Lin, Jenny J. Y.; Low-Nam, Shalini T.; Alfieri, Katherine N.
  • Science Signaling, Vol. 12, Issue 564
  • DOI: 10.1126/scisignal.aat8715

Spectral Identification of Specific Photophysics of Cy5 by Means of Ensemble and Single Molecule Measurements
journal, January 2006

  • Huang, Zhengxi; Ji, Dongmei; Wang, Sufan
  • The Journal of Physical Chemistry A, Vol. 110, Issue 1
  • DOI: 10.1021/jp0562936

A dynamic mode of mitotic bookmarking by transcription factors
journal, November 2016

Photoswitching Mechanism of Cyanine Dyes
journal, December 2009

  • Dempsey, Graham T.; Bates, Mark; Kowtoniuk, Walter E.
  • Journal of the American Chemical Society, Vol. 131, Issue 51
  • DOI: 10.1021/ja904588g

Single-Molecule Studies of Synaptotagmin and Complexin Binding to the SNARE Complex
journal, July 2005

Single-molecule analysis of steroid receptor and cofactor action in living cells
journal, June 2017

  • Paakinaho, Ville; Presman, Diego M.; Ball, David A.
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms15896

Multi-wavelength single-molecule fluorescence analysis of transcription mechanisms
journal, September 2015

Detection of Intensity Change Points in Time-Resolved Single-Molecule Measurements
journal, January 2005

  • Watkins, Lucas P.; Yang, Haw
  • The Journal of Physical Chemistry B, Vol. 109, Issue 1
  • DOI: 10.1021/jp0467548

Structural basis for the phototoxicity of the fluorescent protein KillerRed
journal, July 2009

Single-molecule nanocatalysis reveals heterogeneous reaction pathways and catalytic dynamics
journal, November 2008

  • Xu, Weilin; Kong, Jason S.; Yeh, Yun-Ting E.
  • Nature Materials, Vol. 7, Issue 12
  • DOI: 10.1038/nmat2319

Structural Evidence for a Two-Regime Photobleaching Mechanism in a Reversibly Switchable Fluorescent Protein
journal, October 2013

  • Duan, Chenxi; Adam, Virgile; Byrdin, Martin
  • Journal of the American Chemical Society, Vol. 135, Issue 42
  • DOI: 10.1021/ja406860e

Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function
journal, April 2018

Quantum efficiency independence of the time integrated emission from a fluorescent molecule
journal, January 1976

Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope system
journal, March 1992

Single-molecule DNA repair in live bacteria
journal, April 2013

  • Uphoff, S.; Reyes-Lamothe, R.; Garza de Leon, F.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 20
  • DOI: 10.1073/pnas.1301804110

Photobleaching of Fluorescent Dyes under Conditions Used for Single-Molecule Detection:  Evidence of Two-Step Photolysis
journal, July 1998

  • Eggeling, C.; Widengren, J.; Rigler, R.
  • Analytical Chemistry, Vol. 70, Issue 13
  • DOI: 10.1021/ac980027p

Single-molecule imaging of transcription factor binding to DNA in live mammalian cells
journal, March 2013

  • Gebhardt, J. Christof M.; Suter, David M.; Roy, Rahul
  • Nature Methods, Vol. 10, Issue 5
  • DOI: 10.1038/nmeth.2411

A general mechanism for competitor-induced dissociation of molecular complexes
journal, October 2014

  • Paramanathan, Thayaparan; Reeves, Daniel; Friedman, Larry J.
  • Nature Communications, Vol. 5, Issue 1
  • DOI: 10.1038/ncomms6207

The contribution of singlet oxygen to the photofading of triphenylmethane and related dyes
journal, January 1982

Design of a Highly Sensitive Fluorescent Probe for Interfacial Electron Transfer on a TiO2 Surface
journal, October 2010

  • Tachikawa, Takashi; Wang , Nan; Yamashita, Soichiro
  • Angewandte Chemie International Edition, Vol. 49, Issue 46
  • DOI: 10.1002/anie.201004976

Photobleaching of Rhodamine 6G in Poly(vinyl alcohol) at the Ensemble and Single-Molecule Levels
journal, March 2004

  • Zondervan, Rob; Kulzer, Florian; Kol'chenk, Mikhail A.
  • The Journal of Physical Chemistry A, Vol. 108, Issue 10
  • DOI: 10.1021/jp037222e

Deciphering hierarchical features in the energy landscape of adenylate kinase folding/unfolding
journal, January 2018

  • Taylor, J. Nicholas; Pirchi, Menahem; Haran, Gilad
  • The Journal of Chemical Physics, Vol. 148, Issue 12
  • DOI: 10.1063/1.5016487

Stochastic inhibitor release and binding from single-enzyme molecules
journal, October 2007

  • Gorris, H. H.; Rissin, D. M.; Walt, D. R.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 45
  • DOI: 10.1073/pnas.0705411104

Photobleaching kinetics of fluorescein in quantitative fluorescence microscopy
journal, June 1995

Distinct and Long-Lived Activity States of Single Enzyme Molecules
journal, April 2008

  • Rissin, David M.; Gorris, Hans H.; Walt, David R.
  • Journal of the American Chemical Society, Vol. 130, Issue 15
  • DOI: 10.1021/ja711414f

Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited
journal, December 2005

  • English, Brian P.; Min, Wei; van Oijen, Antoine M.
  • Nature Chemical Biology, Vol. 2, Issue 2
  • DOI: 10.1038/nchembio759

Chromophore Photoreduction in Red Fluorescent Proteins Is Responsible for Bleaching and Phototoxicity
journal, April 2014

  • Vegh, Russell B.; Bravaya, Ksenia B.; Bloch, Dmitry A.
  • The Journal of Physical Chemistry B, Vol. 118, Issue 17
  • DOI: 10.1021/jp500919a

Single-molecule fluorescence spectroscopy maps the folding landscape of a large protein
journal, September 2011

  • Pirchi, Menahem; Ziv, Guy; Riven, Inbal
  • Nature Communications, Vol. 2, Issue 1
  • DOI: 10.1038/ncomms1504

Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed
journal, September 2009

  • Pletnev, Sergei; Gurskaya, Nadya G.; Pletneva, Nadya V.
  • Journal of Biological Chemistry, Vol. 284, Issue 46
  • DOI: 10.1074/jbc.m109.054973

Direct and Sensitized Photooxidation of Cyanine dyes
journal, January 1976

Switch-over of the Mechanism of the Primary Processes in the Photo-oxidation of Xanthene Dyes as Revealed by the Oxygen Consumption Experiments
journal, June 1965

  • Usui, Yoshiharu; Itoh, Keiko; Koizumi, Masao
  • Bulletin of the Chemical Society of Japan, Vol. 38, Issue 6
  • DOI: 10.1246/bcsj.38.1015