SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution

Huang, Aaron; Yao, Helen; Olsen, Bradley D.

doi:10.1039/c9sm00766k

Title: SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution

Journal Article · 01 January 2019 · Soft Matter

DOI: https://doi.org/10.1039/c9sm00766k · OSTI ID:1566261

Huang, Aaron ^[1];

^[1];

^[1]

Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States). Dept. of Chemical Engineering

The interaction between proteins and polymers in solution contributes to numerous important technological processes, including protein crystallization, biofouling, and the self-assembly of protein– polymer bioconjugates. To quantify these interactions, three different polymers—PNIPAM, POEGA, and PDMAPS—were each blended with a model protein mCherry and studied using contrast variation small angle neutron scattering (SANS). This technique allows for the decomposition of the SANS scattering intensity into partial structure factors corresponding to interactions between two polymer chains, interactions between two proteins, and interactions between a polymer chain and a protein, even for concentrations above the overlap concentration. Examining correlations between each component offers insight into the interactions within the system. In particular, mCherry–PNIPAM interactions are consistent with a depletion interaction, and mCherry–POEGA interactions suggest a considerable region of polymer enrichment close to the protein surface, indicative of attractive forces between the two. Interactions between mCherry and PDMAPS are more complex, with possible contributions from both depletion forces and electrostatic forces.

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Research Organization:: Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0007106

OSTI ID:: 1566261

Alternate ID(s):: OSTI ID: 1801762

Journal Information:: Soft Matter, Vol. 15, Issue 37; ISSN 1744-683X

Publisher:: Royal Society of ChemistryCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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References (58)

Principles of protein-protein interactions. Jones, S.; Thornton, J. M. Proceedings of the National Academy of Sciences, Vol. 93, Issue 1 https://doi.org/10.1073/pnas.93.1.13	journal	January 1996
Electrostatics of nanosystems: Application to microtubules and the ribosome Baker, N. A.; Sept, D.; Joseph, S. Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041 https://doi.org/10.1073/pnas.181342398	journal	August 2001
Cooperative Hydration, Chain Collapse, and Flat LCST Behavior in Aqueous Poly( N -isopropylacrylamide) Solutions Okada, Yukinori; Tanaka, Fumihiko Macromolecules, Vol. 38, Issue 10 https://doi.org/10.1021/ma0502497	journal	May 2005
Potential distribution theorem for the polymer-induced depletion between colloidal particles Li, Zhidong; Wu, Jianzhong The Journal of Chemical Physics, Vol. 126, Issue 14 https://doi.org/10.1063/1.2715595	journal	April 2007
Thermosensitive PNIPAM-peptide conjugate – Synthesis and aggregation Trzebicka, Barbara; Robak, Barbara; Trzcinska, Roza European Polymer Journal, Vol. 49, Issue 2 https://doi.org/10.1016/j.eurpolymj.2012.11.005	journal	February 2013
Immobilizing Enzymes: How to Create More Suitable Biocatalysts Bornscheuer, Uwe T. Angewandte Chemie International Edition, Vol. 42, Issue 29 https://doi.org/10.1002/anie.200301664	journal	July 2003
Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods Freedman, A. R.; Galfrè, G.; Gal, E. Journal of Immunological Methods, Vol. 98, Issue 1 https://doi.org/10.1016/0022-1759(87)90445-5	journal	April 1987
Protein-Polymer Hybrid Nanoparticles for Drug Delivery Ge, Jun; Neofytou, Evgenios; Lei, Jiandu Small, Vol. 8, Issue 23 https://doi.org/10.1002/smll.201200889	journal	August 2012
Phase transitions in concentrated solution self-assembly of globular protein–polymer block copolymers Lam, Christopher N.; Olsen, Bradley D. Soft Matter, Vol. 9, Issue 8 https://doi.org/10.1039/c2sm27459k	journal	January 2013
Depletion and pair interactions of proteins in polymer solutions Surve, Megha; Pryamitsyn, Victor; Ganesan, Venkat The Journal of Chemical Physics, Vol. 122, Issue 15 https://doi.org/10.1063/1.1872772	journal	April 2005
Partial structure factors in colloidal silica mixtures determined with small‐angle neutron scattering contrast variation Duits, M. H. G.; May, R. P.; Vrij, A. The Journal of Chemical Physics, Vol. 94, Issue 6 https://doi.org/10.1063/1.460607	journal	March 1991
Responsive Polymer‐Protein Bioconjugates Prepared by RAFT Polymerization and Copper‐Catalyzed Azide‐Alkyne Click Chemistry Li, Ming; De, Priyadarsi; Gondi, Sudershan R. Macromolecular Rapid Communications, Vol. 29, Issue 12–13 https://doi.org/10.1002/marc.200800073	journal	July 2008
Strategies for extended serum half-life of protein therapeutics Kontermann, Roland E. Current Opinion in Biotechnology, Vol. 22, Issue 6 https://doi.org/10.1016/j.copbio.2011.06.012	journal	December 2011
Industrial enzyme applications Kirk, Ole; Borchert, Torben Vedel; Fuglsang, Claus Crone Current Opinion in Biotechnology, Vol. 13, Issue 4 https://doi.org/10.1016/S0958-1669(02)00328-2	journal	August 2002
Protein hydration in solution: Experimental observation by x-ray and neutron scattering Svergun, D. I.; Richard, S.; Koch, M. H. J. Proceedings of the National Academy of Sciences, Vol. 95, Issue 5 https://doi.org/10.1073/pnas.95.5.2267	journal	March 1998
Polysaccharide protein interactions de Kruif, C. G.; Tuinier, R. Food Hydrocolloids, Vol. 15, Issue 4-6 https://doi.org/10.1016/S0268-005X(01)00076-5	journal	July 2001
Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents McBride, Mary T.; Gammon, Stuart; Pitesky, Maurice Analytical Chemistry, Vol. 75, Issue 8 https://doi.org/10.1021/ac026379k	journal	April 2003
The dawning era of polymer therapeutics Duncan, Ruth Nature Reviews Drug Discovery, Vol. 2, Issue 5, p. 347-360 https://doi.org/10.1038/nrd1088	journal	May 2003
Potential applications of enzymes immobilized on/in nano materials: A review Ansari, Shakeel Ahmed; Husain, Qayyum Biotechnology Advances, Vol. 30, Issue 3 https://doi.org/10.1016/j.biotechadv.2011.09.005	journal	May 2012
Gelation Mechanism of Poly( N -isopropylacrylamide)−Clay Nanocomposite Gels Miyazaki, Sho; Endo, Hitoshi; Karino, Takeshi Macromolecules, Vol. 40, Issue 12 https://doi.org/10.1021/ma070104v	journal	June 2007
Highly Active Biocatalytic Coatings from Protein–Polymer Diblock Copolymers Huang, Aaron; Qin, Guokui; Olsen, Bradley D. ACS Applied Materials & Interfaces, Vol. 7, Issue 27 https://doi.org/10.1021/acsami.5b01884	journal	July 2015
Nanostructured materials for applications in drug delivery and tissue engineering Goldberg, Michael; Langer, Robert; Jia, Xinqiao Journal of Biomaterials Science, Polymer Edition, Vol. 18, Issue 3 https://doi.org/10.1163/156856207779996931	journal	January 2007
Irreversible Immobilization of Diisopropylfluorophosphatase in Polyurethane Polymers Drevon, Géraldine F.; Russell, Alan J. Biomacromolecules, Vol. 1, Issue 4 https://doi.org/10.1021/bm0000034	journal	December 2000
Enzymatic hydrolysis optimization to ethanol production by simultaneous saccharification and fermentation Vásquez, Mariana Peñuela; da Silva, Juliana Nascimento C.; de Souza, Maurício Bezerra Applied Biochemistry and Biotechnology, Vol. 137-140, Issue 1-12 https://doi.org/10.1007/s12010-007-9046-2	journal	April 2007
Solid-State Nanostructured Materials from Self-Assembly of a Globular Protein–Polymer Diblock Copolymer Thomas, Carla S.; Glassman, Matthew J.; Olsen, Bradley D. ACS Nano, Vol. 5, Issue 7 https://doi.org/10.1021/nn2013673	journal	June 2011
Computational studies of polyurethanases from Pseudomonas do Canto, Vanessa Petry; Thompson, Claudia Elizabeth; Netz, Paulo Augusto Journal of Molecular Modeling, Vol. 27, Issue 2 https://doi.org/10.1007/s00894-021-04671-x	journal	January 2021
The shape of protein-polymer conjugates in dilute solution Lam, Christopher N.; Chang, Dongsook; Wang, Muzhou Journal of Polymer Science Part A: Polymer Chemistry, Vol. 54, Issue 2 https://doi.org/10.1002/pola.27975	journal	November 2015
Understanding enzyme immobilisation Hanefeld, Ulf; Gardossi, Lucia; Magner, Edmond Chemical Society Reviews, Vol. 38, Issue 2, p. 453-468 https://doi.org/10.1039/B711564B	journal	January 2009
Trehalose Glycopolymers for Stabilization of Protein Conjugates to Environmental Stressors Mancini, Rock J.; Lee, Juneyoung; Maynard, Heather D. Journal of the American Chemical Society, Vol. 134, Issue 20 https://doi.org/10.1021/ja2120234	journal	May 2012
Three‐Dimensional Ordered Antibody Arrays Through Self‐Assembly of Antibody–Polymer Conjugates Dong, Xue‐Hui; Obermeyer, Allie C.; Olsen, Bradley D. Angewandte Chemie, Vol. 129, Issue 5 https://doi.org/10.1002/ange.201607085	journal	December 2016
[FeFe]-Hydrogenase-Catalyzed H ₂ Production in a Photoelectrochemical Biofuel Cell Hambourger, Michael; Gervaldo, Miguel; Svedruzic, Drazenka Journal of the American Chemical Society, Vol. 130, Issue 6 https://doi.org/10.1021/ja077691k	journal	February 2008
Biomaterials for Mediation of Chemical and Biological Warfare Agents Russell, Alan J.; Berberich, Jason A.; Drevon, Géraldine F. Annual Review of Biomedical Engineering, Vol. 5, Issue 1 https://doi.org/10.1146/annurev.bioeng.5.121202.125602	journal	August 2003
Protein−Macromolecule Interactions Odijk, Theo Macromolecules, Vol. 29, Issue 5 https://doi.org/10.1021/ma951467a	journal	January 1996
Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs Broecker, Jana; Eger, Bryan T.; Ernst, Oliver P. Structure, Vol. 25, Issue 2 https://doi.org/10.1016/j.str.2016.12.004	journal	February 2017
Ferrocene-mediated enzyme electrode for amperometric determination of glucose Cass, Anthony E. G.; Davis, Graham.; Francis, Graeme D. Analytical Chemistry, Vol. 56, Issue 4 https://doi.org/10.1021/ac00268a018	journal	April 1984
Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs Broecker, Jana; Eger, Bryan T.; Ernst, Oliver P. Biophysical Journal, Vol. 112, Issue 3 https://doi.org/10.1016/j.bpj.2016.11.1933	journal	February 2017
Immobilizing Enzymes: How to Create More Suitable Biocatalysts. Bornscheuer, Uwe T. ChemInform, Vol. 34, Issue 42 https://doi.org/10.1002/chin.200342037	journal	October 2003
Depletion Interactions in the Protein Limit: Effects of Polymer Density Fluctuations Kulkarni, Amit M.; Chatterjee, Avik P.; Schweizer, Kenneth S. Physical Review Letters, Vol. 83, Issue 22 https://doi.org/10.1103/PhysRevLett.83.4554	journal	November 1999
Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers Bayburt, Timothy H.; Sligar, Stephen G. Protein Science, Vol. 12, Issue 11, p. 2476-2481 https://doi.org/10.1110/ps.03267503	journal	November 2003
SANS studies on catalyst ink of fuel cell Shibayama, Mitsuhiro; Matsunaga, Takuro; Kusano, Takumi Journal of Applied Polymer Science, Vol. 131, Issue 3 https://doi.org/10.1002/app.39842	journal	August 2013
Improvements to the APBS biomolecular solvation software suite: Improvements to the APBS Software Suite Jurrus, Elizabeth; Engel, Dave; Star, Keith Protein Science, Vol. 27, Issue 1 https://doi.org/10.1002/pro.3280	journal	October 2017
Enhanced activity and stability of organophosphorus hydrolase via interaction with an amphiphilic polymer Kim, Minkyu; Gkikas, Manos; Huang, Aaron Chemical Communications, Vol. 50, Issue 40 https://doi.org/10.1039/c3cc47675h	journal	January 2014
Improvements to the APBS biomolecular solvation software suite Jurrus, Elizabeth; Engel, Dave; Star, Keith arXiv https://doi.org/10.48550/arxiv.1707.00027	text	January 2017
Enzyme-based biofuel cells Minteer, Shelley D.; Liaw, Bor Yann; Cooney, Michael J. Current Opinion in Biotechnology, Vol. 18, Issue 3 https://doi.org/10.1016/j.copbio.2007.03.007	journal	June 2007
Structure Analyses of Swollen Rubber-Filler Systems by Using Contrast Variation SANS Takenaka, Mikihito; Nishitsuji, Shotaro; Amino, Naoya Macromolecules, Vol. 42, Issue 1 https://doi.org/10.1021/ma8019046	journal	January 2009
Rapid evolution of mammalian APLP1 as a synaptic adhesion molecule Onodera, Wataru; Asahi, Toru; Sawamura, Naoya Scientific Reports, Vol. 11, Issue 1 https://doi.org/10.1038/s41598-021-90737-y	journal	May 2021
PDB2PQR: an automated pipeline for the setup of Poisson-Boltzmann electrostatics calculations Dolinsky, T. J.; Nielsen, J. E.; McCammon, J. A. Nucleic Acids Research, Vol. 32, Issue Web Server, p. W665-W667 https://doi.org/10.1093/nar/gkh381	journal	July 2004
Three-Dimensional Ordered Antibody Arrays Through Self-Assembly of Antibody-Polymer Conjugates Dong, Xue-Hui; Obermeyer, Allie C.; Olsen, Bradley D. Angewandte Chemie International Edition, Vol. 56, Issue 5 https://doi.org/10.1002/anie.201607085	journal	December 2016
PEGylation of therapeutic proteins JevsÌevar, Simona; Kunstelj, MencÌi; Porekar, Vladka Gaberc Biotechnology Journal, Vol. 5, Issue 1 https://doi.org/10.1002/biot.200900218	journal	January 2010
Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancer Ferrara, Napoleone; Hillan, Kenneth J.; Gerber, Hans-Peter Nature Reviews Drug Discovery, Vol. 3, Issue 5 https://doi.org/10.1038/nrd1381	journal	May 2004
PDB2PQR: expanding and upgrading automated preparation of biomolecular structures for molecular simulations Dolinsky, T. J.; Czodrowski, P.; Li, H. Nucleic Acids Research, Vol. 35, Issue Web Server https://doi.org/10.1093/nar/gkm276	journal	May 2007
Self-assembly of protein-zwitterionic polymer bioconjugates into nanostructured materials Chang, Dongsook; Olsen, Bradley D. Polymer Chemistry, Vol. 7, Issue 13 https://doi.org/10.1039/C5PY01894C	journal	January 2016
Nitrile Hydratase and Its Application to Industrial Production of Acrylamide Yamada, Hideaki; Kobayashi, Michihiko Bioscience, Biotechnology, and Biochemistry, Vol. 60, Issue 9 https://doi.org/10.1271/bbb.60.1391	journal	January 1996
Water-repairable zwitterionic polymer coatings for anti-biofouling surfaces Wang, Zhanhua; van Andel, Esther; Pujari, Sidharam P. Journal of Materials Chemistry B, Vol. 5, Issue 33 https://doi.org/10.1039/C7TB01178D	journal	January 2017
Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly Chang, Dongsook; Lam, Christopher N.; Tang, Shengchang Polym. Chem., Vol. 5, Issue 17 https://doi.org/10.1039/C4PY00448E	journal	January 2014
Enzymes as Catalysts in Synthetic Organic Chemistry [New Synthetic Methods (53)] Whitesides, George M.; Wong, Chi-Huey Angewandte Chemie International Edition in English, Vol. 24, Issue 8 https://doi.org/10.1002/anie.198506173	journal	August 1985
Protein conjugation of thermoresponsive amine-reactive polymers prepared by RAFT Li, Hongmei; Bapat, Abhijeet P.; Li, Ming Polym. Chem., Vol. 2, Issue 2 https://doi.org/10.1039/C0PY00178C	journal	January 2011
Structural origins of diamagnetic anisotropy in proteins. Worcester, D. L. Proceedings of the National Academy of Sciences, Vol. 75, Issue 11 https://doi.org/10.1073/pnas.75.11.5475	journal	November 1978

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Title: SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution

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