SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution
Abstract
The interaction between proteins and polymers in solution contributes to numerous important technological processes, including protein crystallization, biofouling, and the self-assembly of protein– polymer bioconjugates. To quantify these interactions, three different polymers—PNIPAM, POEGA, and PDMAPS—were each blended with a model protein mCherry and studied using contrast variation small angle neutron scattering (SANS). This technique allows for the decomposition of the SANS scattering intensity into partial structure factors corresponding to interactions between two polymer chains, interactions between two proteins, and interactions between a polymer chain and a protein, even for concentrations above the overlap concentration. Examining correlations between each component offers insight into the interactions within the system. In particular, mCherry–PNIPAM interactions are consistent with a depletion interaction, and mCherry–POEGA interactions suggest a considerable region of polymer enrichment close to the protein surface, indicative of attractive forces between the two. Interactions between mCherry and PDMAPS are more complex, with possible contributions from both depletion forces and electrostatic forces.
- Authors:
-
[1];
[1]
- Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, USA
- Publication Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1566261
- Alternate Identifier(s):
- OSTI ID: 1801762
- Grant/Contract Number:
- SC0007106
- Resource Type:
- Published Article
- Journal Name:
- Soft Matter
- Additional Journal Information:
- Journal Name: Soft Matter Journal Volume: 15 Journal Issue: 37; Journal ID: ISSN 1744-683X
- Publisher:
- Royal Society of Chemistry (RSC)
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 36 MATERIALS SCIENCE; Chemistry; Materials Science; Physics; Polymer Science
Citation Formats
Huang, Aaron, Yao, Helen, and Olsen, Bradley D. SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution. United Kingdom: N. p., 2019.
Web. doi:10.1039/C9SM00766K.
Huang, Aaron, Yao, Helen, & Olsen, Bradley D. SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution. United Kingdom. https://doi.org/10.1039/C9SM00766K
Huang, Aaron, Yao, Helen, and Olsen, Bradley D. Wed .
"SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution". United Kingdom. https://doi.org/10.1039/C9SM00766K.
@article{osti_1566261,
title = {SANS partial structure factor analysis for determining protein–polymer interactions in semidilute solution},
author = {Huang, Aaron and Yao, Helen and Olsen, Bradley D.},
abstractNote = {The interaction between proteins and polymers in solution contributes to numerous important technological processes, including protein crystallization, biofouling, and the self-assembly of protein– polymer bioconjugates. To quantify these interactions, three different polymers—PNIPAM, POEGA, and PDMAPS—were each blended with a model protein mCherry and studied using contrast variation small angle neutron scattering (SANS). This technique allows for the decomposition of the SANS scattering intensity into partial structure factors corresponding to interactions between two polymer chains, interactions between two proteins, and interactions between a polymer chain and a protein, even for concentrations above the overlap concentration. Examining correlations between each component offers insight into the interactions within the system. In particular, mCherry–PNIPAM interactions are consistent with a depletion interaction, and mCherry–POEGA interactions suggest a considerable region of polymer enrichment close to the protein surface, indicative of attractive forces between the two. Interactions between mCherry and PDMAPS are more complex, with possible contributions from both depletion forces and electrostatic forces.},
doi = {10.1039/C9SM00766K},
journal = {Soft Matter},
number = 37,
volume = 15,
place = {United Kingdom},
year = {Wed Sep 25 00:00:00 EDT 2019},
month = {Wed Sep 25 00:00:00 EDT 2019}
}
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1039/C9SM00766K
https://doi.org/10.1039/C9SM00766K
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 7 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Principles of protein-protein interactions.
journal, January 1996
- Jones, S.; Thornton, J. M.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 1
Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001
- Baker, N. A.; Sept, D.; Joseph, S.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041
Cooperative Hydration, Chain Collapse, and Flat LCST Behavior in Aqueous Poly( N -isopropylacrylamide) Solutions
journal, May 2005
- Okada, Yukinori; Tanaka, Fumihiko
- Macromolecules, Vol. 38, Issue 10
Potential distribution theorem for the polymer-induced depletion between colloidal particles
journal, April 2007
- Li, Zhidong; Wu, Jianzhong
- The Journal of Chemical Physics, Vol. 126, Issue 14
Thermosensitive PNIPAM-peptide conjugate – Synthesis and aggregation
journal, February 2013
- Trzebicka, Barbara; Robak, Barbara; Trzcinska, Roza
- European Polymer Journal, Vol. 49, Issue 2
Immobilizing Enzymes: How to Create More Suitable Biocatalysts
journal, July 2003
- Bornscheuer, Uwe T.
- Angewandte Chemie International Edition, Vol. 42, Issue 29
Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods
journal, April 1987
- Freedman, A. R.; Galfrè, G.; Gal, E.
- Journal of Immunological Methods, Vol. 98, Issue 1
Protein-Polymer Hybrid Nanoparticles for Drug Delivery
journal, August 2012
- Ge, Jun; Neofytou, Evgenios; Lei, Jiandu
- Small, Vol. 8, Issue 23
Phase transitions in concentrated solution self-assembly of globular protein–polymer block copolymers
journal, January 2013
- Lam, Christopher N.; Olsen, Bradley D.
- Soft Matter, Vol. 9, Issue 8
Depletion and pair interactions of proteins in polymer solutions
journal, April 2005
- Surve, Megha; Pryamitsyn, Victor; Ganesan, Venkat
- The Journal of Chemical Physics, Vol. 122, Issue 15
Partial structure factors in colloidal silica mixtures determined with small‐angle neutron scattering contrast variation
journal, March 1991
- Duits, M. H. G.; May, R. P.; Vrij, A.
- The Journal of Chemical Physics, Vol. 94, Issue 6
Responsive Polymer‐Protein Bioconjugates Prepared by RAFT Polymerization and Copper‐Catalyzed Azide‐Alkyne Click Chemistry
journal, July 2008
- Li, Ming; De, Priyadarsi; Gondi, Sudershan R.
- Macromolecular Rapid Communications, Vol. 29, Issue 12–13
Strategies for extended serum half-life of protein therapeutics
journal, December 2011
- Kontermann, Roland E.
- Current Opinion in Biotechnology, Vol. 22, Issue 6
Industrial enzyme applications
journal, August 2002
- Kirk, Ole; Borchert, Torben Vedel; Fuglsang, Claus Crone
- Current Opinion in Biotechnology, Vol. 13, Issue 4
Protein hydration in solution: Experimental observation by x-ray and neutron scattering
journal, March 1998
- Svergun, D. I.; Richard, S.; Koch, M. H. J.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 5
Polysaccharide protein interactions
journal, July 2001
- de Kruif, C. G.; Tuinier, R.
- Food Hydrocolloids, Vol. 15, Issue 4-6
Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents
journal, April 2003
- McBride, Mary T.; Gammon, Stuart; Pitesky, Maurice
- Analytical Chemistry, Vol. 75, Issue 8
The dawning era of polymer therapeutics
journal, May 2003
- Duncan, Ruth
- Nature Reviews Drug Discovery, Vol. 2, Issue 5, p. 347-360
Potential applications of enzymes immobilized on/in nano materials: A review
journal, May 2012
- Ansari, Shakeel Ahmed; Husain, Qayyum
- Biotechnology Advances, Vol. 30, Issue 3
Gelation Mechanism of Poly( N -isopropylacrylamide)−Clay Nanocomposite Gels
journal, June 2007
- Miyazaki, Sho; Endo, Hitoshi; Karino, Takeshi
- Macromolecules, Vol. 40, Issue 12
Highly Active Biocatalytic Coatings from Protein–Polymer Diblock Copolymers
journal, July 2015
- Huang, Aaron; Qin, Guokui; Olsen, Bradley D.
- ACS Applied Materials & Interfaces, Vol. 7, Issue 27
Nanostructured materials for applications in drug delivery and tissue engineering
journal, January 2007
- Goldberg, Michael; Langer, Robert; Jia, Xinqiao
- Journal of Biomaterials Science, Polymer Edition, Vol. 18, Issue 3
Irreversible Immobilization of Diisopropylfluorophosphatase in Polyurethane Polymers
journal, December 2000
- Drevon, Géraldine F.; Russell, Alan J.
- Biomacromolecules, Vol. 1, Issue 4
Enzymatic hydrolysis optimization to ethanol production by simultaneous saccharification and fermentation
journal, April 2007
- Vásquez, Mariana Peñuela; da Silva, Juliana Nascimento C.; de Souza, Maurício Bezerra
- Applied Biochemistry and Biotechnology, Vol. 137-140, Issue 1-12
Solid-State Nanostructured Materials from Self-Assembly of a Globular Protein–Polymer Diblock Copolymer
journal, June 2011
- Thomas, Carla S.; Glassman, Matthew J.; Olsen, Bradley D.
- ACS Nano, Vol. 5, Issue 7
The shape of protein-polymer conjugates in dilute solution
journal, November 2015
- Lam, Christopher N.; Chang, Dongsook; Wang, Muzhou
- Journal of Polymer Science Part A: Polymer Chemistry, Vol. 54, Issue 2
Understanding enzyme immobilisation
journal, January 2009
- Hanefeld, Ulf; Gardossi, Lucia; Magner, Edmond
- Chemical Society Reviews, Vol. 38, Issue 2, p. 453-468
Trehalose Glycopolymers for Stabilization of Protein Conjugates to Environmental Stressors
journal, May 2012
- Mancini, Rock J.; Lee, Juneyoung; Maynard, Heather D.
- Journal of the American Chemical Society, Vol. 134, Issue 20
[FeFe]-Hydrogenase-Catalyzed H 2 Production in a Photoelectrochemical Biofuel Cell
journal, February 2008
- Hambourger, Michael; Gervaldo, Miguel; Svedruzic, Drazenka
- Journal of the American Chemical Society, Vol. 130, Issue 6
Biomaterials for Mediation of Chemical and Biological Warfare Agents
journal, August 2003
- Russell, Alan J.; Berberich, Jason A.; Drevon, Géraldine F.
- Annual Review of Biomedical Engineering, Vol. 5, Issue 1
Protein−Macromolecule Interactions
journal, January 1996
- Odijk, Theo
- Macromolecules, Vol. 29, Issue 5
Crystallogenesis of Membrane Proteins Mediated by Polymer-Bounded Lipid Nanodiscs
journal, February 2017
- Broecker, Jana; Eger, Bryan T.; Ernst, Oliver P.
- Structure, Vol. 25, Issue 2
Ferrocene-mediated enzyme electrode for amperometric determination of glucose
journal, April 1984
- Cass, Anthony E. G.; Davis, Graham.; Francis, Graeme D.
- Analytical Chemistry, Vol. 56, Issue 4
Depletion Interactions in the Protein Limit: Effects of Polymer Density Fluctuations
journal, November 1999
- Kulkarni, Amit M.; Chatterjee, Avik P.; Schweizer, Kenneth S.
- Physical Review Letters, Vol. 83, Issue 22
Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003
- Bayburt, Timothy H.; Sligar, Stephen G.
- Protein Science, Vol. 12, Issue 11, p. 2476-2481
SANS studies on catalyst ink of fuel cell
journal, August 2013
- Shibayama, Mitsuhiro; Matsunaga, Takuro; Kusano, Takumi
- Journal of Applied Polymer Science, Vol. 131, Issue 3
Improvements to the APBS biomolecular solvation software suite: Improvements to the APBS Software Suite
journal, October 2017
- Jurrus, Elizabeth; Engel, Dave; Star, Keith
- Protein Science, Vol. 27, Issue 1
Enhanced activity and stability of organophosphorus hydrolase via interaction with an amphiphilic polymer
journal, January 2014
- Kim, Minkyu; Gkikas, Manos; Huang, Aaron
- Chemical Communications, Vol. 50, Issue 40
Enzyme-based biofuel cells
journal, June 2007
- Minteer, Shelley D.; Liaw, Bor Yann; Cooney, Michael J.
- Current Opinion in Biotechnology, Vol. 18, Issue 3
Structure Analyses of Swollen Rubber-Filler Systems by Using Contrast Variation SANS
journal, January 2009
- Takenaka, Mikihito; Nishitsuji, Shotaro; Amino, Naoya
- Macromolecules, Vol. 42, Issue 1
PDB2PQR: an automated pipeline for the setup of Poisson-Boltzmann electrostatics calculations
journal, July 2004
- Dolinsky, T. J.; Nielsen, J. E.; McCammon, J. A.
- Nucleic Acids Research, Vol. 32, Issue Web Server, p. W665-W667
Three-Dimensional Ordered Antibody Arrays Through Self-Assembly of Antibody-Polymer Conjugates
journal, December 2016
- Dong, Xue-Hui; Obermeyer, Allie C.; Olsen, Bradley D.
- Angewandte Chemie International Edition, Vol. 56, Issue 5
PEGylation of therapeutic proteins
journal, January 2010
- JevsÌevar, Simona; Kunstelj, MencÌi; Porekar, Vladka Gaberc
- Biotechnology Journal, Vol. 5, Issue 1
Discovery and development of bevacizumab, an anti-VEGF antibody for treating cancer
journal, May 2004
- Ferrara, Napoleone; Hillan, Kenneth J.; Gerber, Hans-Peter
- Nature Reviews Drug Discovery, Vol. 3, Issue 5
PDB2PQR: expanding and upgrading automated preparation of biomolecular structures for molecular simulations
journal, May 2007
- Dolinsky, T. J.; Czodrowski, P.; Li, H.
- Nucleic Acids Research, Vol. 35, Issue Web Server
Self-assembly of protein-zwitterionic polymer bioconjugates into nanostructured materials
journal, January 2016
- Chang, Dongsook; Olsen, Bradley D.
- Polymer Chemistry, Vol. 7, Issue 13
Nitrile Hydratase and Its Application to Industrial Production of Acrylamide
journal, January 1996
- Yamada, Hideaki; Kobayashi, Michihiko
- Bioscience, Biotechnology, and Biochemistry, Vol. 60, Issue 9
Water-repairable zwitterionic polymer coatings for anti-biofouling surfaces
journal, January 2017
- Wang, Zhanhua; van Andel, Esther; Pujari, Sidharam P.
- Journal of Materials Chemistry B, Vol. 5, Issue 33
Effect of polymer chemistry on globular protein–polymer block copolymer self-assembly
journal, January 2014
- Chang, Dongsook; Lam, Christopher N.; Tang, Shengchang
- Polym. Chem., Vol. 5, Issue 17
Enzymes as Catalysts in Synthetic Organic Chemistry [New Synthetic Methods (53)]
journal, August 1985
- Whitesides, George M.; Wong, Chi-Huey
- Angewandte Chemie International Edition in English, Vol. 24, Issue 8
Protein conjugation of thermoresponsive amine-reactive polymers prepared by RAFT
journal, January 2011
- Li, Hongmei; Bapat, Abhijeet P.; Li, Ming
- Polym. Chem., Vol. 2, Issue 2
Structural origins of diamagnetic anisotropy in proteins.
journal, November 1978
- Worcester, D. L.
- Proceedings of the National Academy of Sciences, Vol. 75, Issue 11