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Title: Cytoprotective metal-organic frameworks for anaerobic bacteria

Abstract

We report a strategy to uniformly wrap Morella thermoacetica bacteria with a metal-organic framework (MOF) monolayer of nanometer thickness for cytoprotection in artificial photosynthesis. The catalytic activity of the MOF enclosure toward decomposition of reactive oxygen species (ROS) reduces the death of strictly anaerobic bacteria by fivefold in the presence of 21% O2, and enables the cytoprotected bacteria to continuously produce acetate from CO2 fixation under oxidative stress. The high definition of the MOF–bacteria interface involving direct bonding between phosphate units on the cell surface and zirconium clusters on MOF monolayer, provides for enhancement of life throughout reproduction. The dynamic nature of the MOF wrapping allows for cell elongation and separation, including spontaneous covering of the newly grown cell surface. Finally, the open-metal sites on the zirconium clusters lead to 600 times more efficient ROS decomposition compared with zirconia nanoparticles.

Authors:
 [1];  [2];  [2];  [3];  [4]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division; Kavli Energy NanoSciences Inst., Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
  3. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division; King Abdulaziz City of Science and Technology, Riyadh (Saudi Arabia)
  4. Univ. of California, Berkeley, CA (United States). Dept. of Chemistry; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division; Univ. of California, Berkeley, CA (United States). Dept. of Materials Science and Engineering; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). fChemical Sciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC); National Aeronautics and Space Administration (NASA)
OSTI Identifier:
1561896
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 115; Journal Issue: 42; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; cell wrapping; metal-organic frameworks; anaerobic bacteria; artificial photosynthesis; reactive oxygen species

Citation Formats

Ji, Zhe, Zhang, Hao, Liu, Hao, Yaghi, Omar M., and Yang, Peidong. Cytoprotective metal-organic frameworks for anaerobic bacteria. United States: N. p., 2018. Web. doi:10.1073/pnas.1808829115.
Ji, Zhe, Zhang, Hao, Liu, Hao, Yaghi, Omar M., & Yang, Peidong. Cytoprotective metal-organic frameworks for anaerobic bacteria. United States. doi:10.1073/pnas.1808829115.
Ji, Zhe, Zhang, Hao, Liu, Hao, Yaghi, Omar M., and Yang, Peidong. Mon . "Cytoprotective metal-organic frameworks for anaerobic bacteria". United States. doi:10.1073/pnas.1808829115. https://www.osti.gov/servlets/purl/1561896.
@article{osti_1561896,
title = {Cytoprotective metal-organic frameworks for anaerobic bacteria},
author = {Ji, Zhe and Zhang, Hao and Liu, Hao and Yaghi, Omar M. and Yang, Peidong},
abstractNote = {We report a strategy to uniformly wrap Morella thermoacetica bacteria with a metal-organic framework (MOF) monolayer of nanometer thickness for cytoprotection in artificial photosynthesis. The catalytic activity of the MOF enclosure toward decomposition of reactive oxygen species (ROS) reduces the death of strictly anaerobic bacteria by fivefold in the presence of 21% O2, and enables the cytoprotected bacteria to continuously produce acetate from CO2 fixation under oxidative stress. The high definition of the MOF–bacteria interface involving direct bonding between phosphate units on the cell surface and zirconium clusters on MOF monolayer, provides for enhancement of life throughout reproduction. The dynamic nature of the MOF wrapping allows for cell elongation and separation, including spontaneous covering of the newly grown cell surface. Finally, the open-metal sites on the zirconium clusters lead to 600 times more efficient ROS decomposition compared with zirconia nanoparticles.},
doi = {10.1073/pnas.1808829115},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 42,
volume = 115,
place = {United States},
year = {2018},
month = {10}
}

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Cited by: 29 works
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    Nanocoating of Single Cells: From Maintenance of Cell Viability to Manipulation of Cellular Activities
    journal, January 2014


    Metal-Organic Framework Coatings as Cytoprotective Exoskeletons for Living Cells
    journal, July 2016

    • Liang, Kang; Richardson, Joseph J.; Cui, Jiwei
    • Advanced Materials, Vol. 28, Issue 36
    • DOI: 10.1002/adma.201602335

    Biomimetic Encapsulation of Individual Cells with Silica
    journal, November 2009

    • Yang, Sung Ho; Lee, Kyung-Bok; Kong, Bokyung
    • Angewandte Chemie International Edition, Vol. 48, Issue 48
    • DOI: 10.1002/anie.200903010

    Self-Supporting Metal-Organic Layers as Single-Site Solid Catalysts
    journal, March 2016

    • Cao, Lingyun; Lin, Zekai; Peng, Fei
    • Angewandte Chemie International Edition, Vol. 55, Issue 16
    • DOI: 10.1002/anie.201512054

    An Enzyme-Coated Metal-Organic Framework Shell for Synthetically Adaptive Cell Survival
    journal, June 2017

    • Liang, Kang; Richardson, Joseph J.; Doonan, Christian J.
    • Angewandte Chemie International Edition, Vol. 56, Issue 29
    • DOI: 10.1002/anie.201704120

    Nanowire–Bacteria Hybrids for Unassisted Solar Carbon Dioxide Fixation to Value-Added Chemicals
    journal, April 2015


    Cysteine–Cystine Photoregeneration for Oxygenic Photosynthesis of Acetic Acid from CO 2 by a Tandem Inorganic–Biological Hybrid System
    journal, August 2016


    Electrostatic Layer-by-Layer Nanoassembly on Biological Microtemplates:  Platelets
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    Characterization of Zirconium Phosphate/Polycation Thin Films Grown by Sequential Adsorption Reactions
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    Self-photosensitization of nonphotosynthetic bacteria for solar-to-chemical production
    journal, December 2015


    Water splitting–biosynthetic system with CO 2 reduction efficiencies exceeding photosynthesis
    journal, June 2016


    Tolerance and Metabolic Response of Acetogenic Bacteria toward Oxygen
    journal, February 2002


    Hydrogen-Dependent Oxygen Reduction by Homoacetogenic Bacteria Isolated from Termite Guts
    journal, February 2003


    Cytochrome bd Oxidase, Oxidative Stress, and Dioxygen Tolerance of the Strictly Anaerobic Bacterium Moorella thermoacetica
    journal, March 2005


    Wall Teichoic Acids of Gram-Positive Bacteria
    journal, September 2013


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    journal, October 1980


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