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Title: Dose‐dependent study of effects of 532‐nm continuous wave laser on rat skin: A mechanistic insight

Abstract

Abstract Visible lasers emitting in the green spectral region are being routinely employed in various medical and defense fields namely treatment of pigmented lesions, tattoo inks, port wine stains, dazzling the target or mob dispersal. Despite their increasing applications, lasers also tend to pose occupational hazards to operators, ancillary personnel, individuals undergoing laser therapies. This study was aimed at investigating the effects of different doses of 532‐nm continuous wave laser on rat skin. The present study demonstrated that higher fluences of 532‐nm continuous wave (CW) laser induces significant tissue damage through induction of tumor necrosis factor‐α, cyclooxygenase‐2, tumor protein (p53), PARP 1, caspase3 which in turn leads to tissue damage and cell death. Furthermore, level of heat shock proteins, pAkt were found up‐regulated as a cope up response to laser‐induced stress. On the basis of the findings, irradiation with 532‐nm CW laser up to 2.5 J/cm 2 was found within the safe exposure limits. Thus, it is probably the first attempt to demonstrate the tissue damage induced by 532‐nm CW laser on skin, which may help in choosing safe laser dose for certain skin‐based applications and evolving methods to ameliorate laser‐inflicted injuries.

Authors:
 [1]; ORCiD logo [1];  [2];  [2]
  1. Defence Institute of Physiology and Allied Sciences (DIPAS) DRDO Delhi India
  2. Laser Science and Technology Centre (LASTEC) DRDO Delhi India
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1559367
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Biophotonics
Additional Journal Information:
Journal Name: Journal of Biophotonics Journal Volume: 12 Journal Issue: 10; Journal ID: ISSN 1864-063X
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Germany
Language:
English

Citation Formats

Bora, Kiran, Kumar, Bhuvnesh, Prakash, Satya, and Rathi, Ajay. Dose‐dependent study of effects of 532‐nm continuous wave laser on rat skin: A mechanistic insight. Germany: N. p., 2019. Web. doi:10.1002/jbio.201800484.
Bora, Kiran, Kumar, Bhuvnesh, Prakash, Satya, & Rathi, Ajay. Dose‐dependent study of effects of 532‐nm continuous wave laser on rat skin: A mechanistic insight. Germany. https://doi.org/10.1002/jbio.201800484
Bora, Kiran, Kumar, Bhuvnesh, Prakash, Satya, and Rathi, Ajay. Thu . "Dose‐dependent study of effects of 532‐nm continuous wave laser on rat skin: A mechanistic insight". Germany. https://doi.org/10.1002/jbio.201800484.
@article{osti_1559367,
title = {Dose‐dependent study of effects of 532‐nm continuous wave laser on rat skin: A mechanistic insight},
author = {Bora, Kiran and Kumar, Bhuvnesh and Prakash, Satya and Rathi, Ajay},
abstractNote = {Abstract Visible lasers emitting in the green spectral region are being routinely employed in various medical and defense fields namely treatment of pigmented lesions, tattoo inks, port wine stains, dazzling the target or mob dispersal. Despite their increasing applications, lasers also tend to pose occupational hazards to operators, ancillary personnel, individuals undergoing laser therapies. This study was aimed at investigating the effects of different doses of 532‐nm continuous wave laser on rat skin. The present study demonstrated that higher fluences of 532‐nm continuous wave (CW) laser induces significant tissue damage through induction of tumor necrosis factor‐α, cyclooxygenase‐2, tumor protein (p53), PARP 1, caspase3 which in turn leads to tissue damage and cell death. Furthermore, level of heat shock proteins, pAkt were found up‐regulated as a cope up response to laser‐induced stress. On the basis of the findings, irradiation with 532‐nm CW laser up to 2.5 J/cm 2 was found within the safe exposure limits. Thus, it is probably the first attempt to demonstrate the tissue damage induced by 532‐nm CW laser on skin, which may help in choosing safe laser dose for certain skin‐based applications and evolving methods to ameliorate laser‐inflicted injuries.},
doi = {10.1002/jbio.201800484},
journal = {Journal of Biophotonics},
number = 10,
volume = 12,
place = {Germany},
year = {2019},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1002/jbio.201800484

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