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Title: Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat

Abstract

Fusarium crown rot (FCR) of wheat and barley, predominantly caused by the fungal pathogen Fusarium pseudograminearum, is a disease of economic significance. The quantitative nature of FCR resistance within cultivated wheat germplasm has significantly limited breeding efforts to enhanced FCR resistance in wheat. In this study, we characterized the molecular responses of Brachypodium distachyon (Brachypodium hereafter) to F. pseudograminearum infection using RNA-seq to determine whether Brachypodium can be exploited as a model system towards better understanding of F. pseudograminearum-wheat interaction. The transcriptional response to infection in Brachypodium was strikingly similar to that previously reported in wheat, both in shared expression patterns of wheat homologs of Brachypodium genes and functional overlap revealed through comparative gene ontology analysis in both species. Metabolites produced by various biosynthetic pathways induced in both wheat and Brachypodium were quantified, revealing a high degree of overlap between these two species in metabolic response to infection but also showed Brachypodium does not produce certain defence-related metabolites found in wheat. Functional analyses of candidate genes identified in this study will improve our understanding of resistance mechanisms and may lead to the development of new strategies to protect cereal crops from pathogen infection.

Authors:
 [1];  [2];  [3];  [2];  [2]; ORCiD logo [2]; ORCiD logo [2];  [4];  [5];  [6];  [1]
  1. Commonwealth Scientific and Industrial Research Organization Agriculture and Food (CSIRO), St Lucia, QLD (Australia); Univ. of Queensland, St Lucia, QLD (Australia)
  2. Commonwealth Scientific and Industrial Research Organization Agriculture and Food (CSIRO), St Lucia, QLD (Australia)
  3. Univ. of Technology Sydney (Australia)
  4. Commonwealth Scientific and Industrial Research Organization (CSIRO), Dickson ACT (Australia)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Univ. of Queensland, St Lucia, QLD (Australia)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC); Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1543778
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Powell, Jonathan J., Carere, Jason, Sablok, Gaurav, Fitzgerald, Timothy L., Stiller, Jiri, Colgrave, Michelle L., Gardiner, Donald M., Manners, John M., Vogel, John P., Henry, Robert J., and Kazan, Kemal. Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat. United States: N. p., 2017. Web. doi:10.1038/s41598-017-17454-3.
Powell, Jonathan J., Carere, Jason, Sablok, Gaurav, Fitzgerald, Timothy L., Stiller, Jiri, Colgrave, Michelle L., Gardiner, Donald M., Manners, John M., Vogel, John P., Henry, Robert J., & Kazan, Kemal. Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat. United States. doi:10.1038/s41598-017-17454-3.
Powell, Jonathan J., Carere, Jason, Sablok, Gaurav, Fitzgerald, Timothy L., Stiller, Jiri, Colgrave, Michelle L., Gardiner, Donald M., Manners, John M., Vogel, John P., Henry, Robert J., and Kazan, Kemal. Fri . "Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat". United States. doi:10.1038/s41598-017-17454-3. https://www.osti.gov/servlets/purl/1543778.
@article{osti_1543778,
title = {Transcriptome analysis of Brachypodium during fungal pathogen infection reveals both shared and distinct defense responses with wheat},
author = {Powell, Jonathan J. and Carere, Jason and Sablok, Gaurav and Fitzgerald, Timothy L. and Stiller, Jiri and Colgrave, Michelle L. and Gardiner, Donald M. and Manners, John M. and Vogel, John P. and Henry, Robert J. and Kazan, Kemal},
abstractNote = {Fusarium crown rot (FCR) of wheat and barley, predominantly caused by the fungal pathogen Fusarium pseudograminearum, is a disease of economic significance. The quantitative nature of FCR resistance within cultivated wheat germplasm has significantly limited breeding efforts to enhanced FCR resistance in wheat. In this study, we characterized the molecular responses of Brachypodium distachyon (Brachypodium hereafter) to F. pseudograminearum infection using RNA-seq to determine whether Brachypodium can be exploited as a model system towards better understanding of F. pseudograminearum-wheat interaction. The transcriptional response to infection in Brachypodium was strikingly similar to that previously reported in wheat, both in shared expression patterns of wheat homologs of Brachypodium genes and functional overlap revealed through comparative gene ontology analysis in both species. Metabolites produced by various biosynthetic pathways induced in both wheat and Brachypodium were quantified, revealing a high degree of overlap between these two species in metabolic response to infection but also showed Brachypodium does not produce certain defence-related metabolites found in wheat. Functional analyses of candidate genes identified in this study will improve our understanding of resistance mechanisms and may lead to the development of new strategies to protect cereal crops from pathogen infection.},
doi = {10.1038/s41598-017-17454-3},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {12}
}

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