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Title: Evolution of a Vegetarian Vibrio: Metabolic Specialization of Vibrio breoganii to Macroalgal Substrates

Abstract

While most Vibrionaceae are considered generalists that thrive on diverse substrates, including animal-derived material, we show that Vibrio breoganii has specialized for the consumption of marine macroalga-derived substrates. Genomic and physiological comparisons of V. breoganii with other Vibrionaceae isolates revealed the ability to degrade alginate, laminarin, and additional glycans present in algal cell walls. Moreover, the widely conserved ability to hydrolyze animal-derived polymers, including chitin and glycogen, was lost, along with the ability to efficiently grow on a variety of amino acids. Ecological data showing associations with particulate algal material but not zooplankton further support this shift in niche preference, and the loss of motility appears to reflect a sessile macroalga-associated lifestyle. Altogether, these findings indicate that algal polysaccharides have become a major source of carbon and energy in V. breoganii, and these ecophysiological adaptations may facilitate transient commensal associations with marine invertebrates that feed on algae.

Authors:
 [1];  [1];  [1];  [2];  [3];  [4];  [1];  [5]; ORCiD logo [1];  [6]
  1. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  2. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Univ. of Bremen Max Planck Inst. for Marine Microbiology, Bremen (Germany)
  3. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Khalifa Univ. of Science and Technology, Abu Dhabi (United Arab of Emirates)
  4. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Johns Hopkins Univ., Baltimore, MD (United States)
  5. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Broad Inst., Cambridge, MA (United States)
  6. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1541705
Grant/Contract Number:  
SC0008743
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Bacteriology
Additional Journal Information:
Journal Volume: 200; Journal Issue: 15; Journal ID: ISSN 0021-9193
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; microbiology; macroalgal carbohydrates; horizontal gene transfer; metabolic specialization; Vibrionaceae; ecology; adaptation; macroalgae; seaweed; Vibrio; algae; degradation; polysaccharide

Citation Formats

Corzett, Christopher H., Elsherbini, Joseph, Chien, Diana M., Hehemann, Jan-Hendrik, Henschel, Andreas, Preheim, Sarah P., Yu, Xiaoqian, Alm, Eric J., Polz, Martin F., and DiRita, Victor J. Evolution of a Vegetarian Vibrio: Metabolic Specialization of Vibrio breoganii to Macroalgal Substrates. United States: N. p., 2018. Web. doi:10.1128/jb.00020-18.
Corzett, Christopher H., Elsherbini, Joseph, Chien, Diana M., Hehemann, Jan-Hendrik, Henschel, Andreas, Preheim, Sarah P., Yu, Xiaoqian, Alm, Eric J., Polz, Martin F., & DiRita, Victor J. Evolution of a Vegetarian Vibrio: Metabolic Specialization of Vibrio breoganii to Macroalgal Substrates. United States. doi:10.1128/jb.00020-18.
Corzett, Christopher H., Elsherbini, Joseph, Chien, Diana M., Hehemann, Jan-Hendrik, Henschel, Andreas, Preheim, Sarah P., Yu, Xiaoqian, Alm, Eric J., Polz, Martin F., and DiRita, Victor J. Mon . "Evolution of a Vegetarian Vibrio: Metabolic Specialization of Vibrio breoganii to Macroalgal Substrates". United States. doi:10.1128/jb.00020-18. https://www.osti.gov/servlets/purl/1541705.
@article{osti_1541705,
title = {Evolution of a Vegetarian Vibrio: Metabolic Specialization of Vibrio breoganii to Macroalgal Substrates},
author = {Corzett, Christopher H. and Elsherbini, Joseph and Chien, Diana M. and Hehemann, Jan-Hendrik and Henschel, Andreas and Preheim, Sarah P. and Yu, Xiaoqian and Alm, Eric J. and Polz, Martin F. and DiRita, Victor J.},
abstractNote = {While most Vibrionaceae are considered generalists that thrive on diverse substrates, including animal-derived material, we show that Vibrio breoganii has specialized for the consumption of marine macroalga-derived substrates. Genomic and physiological comparisons of V. breoganii with other Vibrionaceae isolates revealed the ability to degrade alginate, laminarin, and additional glycans present in algal cell walls. Moreover, the widely conserved ability to hydrolyze animal-derived polymers, including chitin and glycogen, was lost, along with the ability to efficiently grow on a variety of amino acids. Ecological data showing associations with particulate algal material but not zooplankton further support this shift in niche preference, and the loss of motility appears to reflect a sessile macroalga-associated lifestyle. Altogether, these findings indicate that algal polysaccharides have become a major source of carbon and energy in V. breoganii, and these ecophysiological adaptations may facilitate transient commensal associations with marine invertebrates that feed on algae.},
doi = {10.1128/jb.00020-18},
journal = {Journal of Bacteriology},
number = 15,
volume = 200,
place = {United States},
year = {2018},
month = {4}
}

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