Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale

Abstract

Hydraulic fracturing is a prominent method of natural gas production that uses injected, high-pressure fluids to fracture low permeability, hydrocarbon rich strata such as shale. Upon completion of a well, the fluid returns to the surface (produced water) and contains natural gas, subsurface constituents, and microorganisms (Barbot et al., 2013; Daly et al., 2016). While the microbial community of the produced fluids has been studied in multiple gas wells, the activity of these microorganisms and their relation to biogeochemical activity is not well understood. In this experiment, we supplemented produced fluid with 13C-labeled carbon sources (glucose, acetate, bicarbonate, methanol, or methane), and 15N-labeled ammonium chloride in order to isotopically trace microbial activity over multiple day in anoxic incubations. Nanoscale secondary ion mass spectrometry (NanoSIMS) was used to generate isotopic images of 13C and 15N incorporation in individual cells, while isotope ratio monitoring–gas chromatography–mass spectrometry (IRM–GC–MS) was used to measure 13CO2, and 13CH4 as metabolic byproducts. Glucose, acetate, and methanol were all assimilated by microorganisms under anoxic conditions. 13CO2 production was only observed with glucose as a substrate indicating that catabolic activity was limited to this condition. The microbial communities observed at 0, 19, and 32 days of incubation did notmore » vary between different carbon sources, were low in diversity, and composed primarily of the class Clostridia. The primary genera detected in the incubations, Halanaerobium and Fusibacter, are known to be adapted to harsh physical and chemical conditions consistent with those that occur in the hydrofracturing environment. This study provides evidence that microorganisms in produced fluid are revivable in laboratory incubations and retained the ability to metabolize added carbon and nitrogen substrates.« less

Authors:
 [1];  [2];  [1];  [1];  [1];  [3];  [2];  [4];  [5];  [3]
+ Show Author Affiliations
  1. Japan Agency for Marine-Earth Science and Technology, Kochi (Japan); Japan Agency for Marine-Earth Science and Technology, Yokosuka (Japan)
  2. National Energy Technology Lab. (NETL), Albany, OR (United States)
  3. Oregon State Univ., Corvallis, OR (United States)
  4. Marine Works Japan, Yokosuka (Japan)
  5. Japan Agency for Marine-Earth Science and Technology, Kochi (Japan); Japan Agency for Marine-Earth Science and Technology, Yokosuka (Japan); Japan Agency for Marine-Earth Science and Technology, Yokohama (Japan)
Publication Date:
Research Org.:
National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV, and Albany, OR (United States)
Sponsoring Org.:
FE; USDOE
OSTI Identifier:
1532759
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal Issue: n/a; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Morono, Yuki, Wishart, Jessie R., Ito, Motoo, Ijiri, Akira, Hoshino, Tatsuhiko, Torres, Marta, Verba, Circe, Terada, Takeshi, Inagaki, Fumio, and Colwell, Frederick S. Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.00376.
Copy to clipboard
Morono, Yuki, Wishart, Jessie R., Ito, Motoo, Ijiri, Akira, Hoshino, Tatsuhiko, Torres, Marta, Verba, Circe, Terada, Takeshi, Inagaki, Fumio, & Colwell, Frederick S. Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale. United States. https://doi.org/10.3389/fmicb.2019.00376
Copy to clipboard
Morono, Yuki, Wishart, Jessie R., Ito, Motoo, Ijiri, Akira, Hoshino, Tatsuhiko, Torres, Marta, Verba, Circe, Terada, Takeshi, Inagaki, Fumio, and Colwell, Frederick S. Tue . "Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale". United States. https://doi.org/10.3389/fmicb.2019.00376. https://www.osti.gov/servlets/purl/1532759.
Copy to clipboard
@article{osti_1532759,
title = {Microbial Metabolism and Community Dynamics in Hydraulic Fracturing Fluids Recovered From Deep Hydrocarbon-Rich Shale},
author = {Morono, Yuki and Wishart, Jessie R. and Ito, Motoo and Ijiri, Akira and Hoshino, Tatsuhiko and Torres, Marta and Verba, Circe and Terada, Takeshi and Inagaki, Fumio and Colwell, Frederick S.},
abstractNote = {Hydraulic fracturing is a prominent method of natural gas production that uses injected, high-pressure fluids to fracture low permeability, hydrocarbon rich strata such as shale. Upon completion of a well, the fluid returns to the surface (produced water) and contains natural gas, subsurface constituents, and microorganisms (Barbot et al., 2013; Daly et al., 2016). While the microbial community of the produced fluids has been studied in multiple gas wells, the activity of these microorganisms and their relation to biogeochemical activity is not well understood. In this experiment, we supplemented produced fluid with 13C-labeled carbon sources (glucose, acetate, bicarbonate, methanol, or methane), and 15N-labeled ammonium chloride in order to isotopically trace microbial activity over multiple day in anoxic incubations. Nanoscale secondary ion mass spectrometry (NanoSIMS) was used to generate isotopic images of 13C and 15N incorporation in individual cells, while isotope ratio monitoring–gas chromatography–mass spectrometry (IRM–GC–MS) was used to measure 13CO2, and 13CH4 as metabolic byproducts. Glucose, acetate, and methanol were all assimilated by microorganisms under anoxic conditions. 13CO2 production was only observed with glucose as a substrate indicating that catabolic activity was limited to this condition. The microbial communities observed at 0, 19, and 32 days of incubation did not vary between different carbon sources, were low in diversity, and composed primarily of the class Clostridia. The primary genera detected in the incubations, Halanaerobium and Fusibacter, are known to be adapted to harsh physical and chemical conditions consistent with those that occur in the hydrofracturing environment. This study provides evidence that microorganisms in produced fluid are revivable in laboratory incubations and retained the ability to metabolize added carbon and nitrogen substrates.},
doi = {10.3389/fmicb.2019.00376},
journal = {Frontiers in Microbiology},
number = n/a,
volume = 10,
place = {United States},
year = {Tue Mar 12 00:00:00 EDT 2019},
month = {Tue Mar 12 00:00:00 EDT 2019}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.3389/fmicb.2019.00376
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 11 works
Citation information provided by
Web of Science

Figures / Tables:

FIGURE 1 FIGURE 1: Serum bottle containing produced water as prepared for anoxic incubations.
All figures and tables (7 total)
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Spatial and Temporal Correlation of Water Quality Parameters of Produced Waters from Devonian-Age Shale following Hydraulic Fracturing
journal, February 2013

  • Barbot, Elise; Vidic, Natasa S.; Gregory, Kelvin B.
  • Environmental Science & Technology, Vol. 47, Issue 6
  • DOI: 10.1021/es304638h

Physical, chemical, and biological characteristics of compounds used in hydraulic fracturing
journal, June 2014

Wastewater management and Marcellus Shale gas development: Trends, drivers, and planning implications
journal, May 2013

Fusibacter bizertensis sp. nov., isolated from a corroded kerosene storage tank
journal, October 2014

  • Smii, L.; Ben Hania, W.; Cayol, J. -L.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 65, Issue Pt 1
  • DOI: 10.1099/ijs.0.066183-0

Distribution of the phosphoenolpyruvate:glucose phosphotransferase system in fermentative bacteria
journal, July 1979

Microbial activity in the marine deep biosphere: progress and prospects
journal, January 2013

  • Orcutt, Beth N.; LaRowe, Douglas E.; Biddle, Jennifer F.
  • Frontiers in Microbiology, Vol. 4
  • DOI: 10.3389/fmicb.2013.00189

Distribution of the Phosphoenolpyruvate:Glucose Phosphotransferase System in Bacteria
journal, November 1970

Naturally Occurring Asbestos in Valmalenco (Central Alps, Northern Italy): From Quarries and Mines to Stream Sediments
journal, February 2020

  • Cavallo, Alessandro; Petriglieri, Jasmine Rita
  • Environmental and Engineering Geoscience, Vol. 26, Issue 1
  • DOI: 10.2113/eeg-2270

Produced and Flowback Water Recycling and Reuse: Economics, Limitations, and Technology
journal, February 2014

Characterization of hydraulic fracturing flowback water in Colorado: Implications for water treatment
journal, April 2015

Formation Damage and Impact on Gas Flow Caused by Biofilms Growing Within Proppant Packing Used in Hydraulic Fracturing
conference, April 2013

  • Bottero, Simona; Picioreanu, Cristian; Enzien, Michael V.
  • SPE International Symposium and Exhibition on Formation Damage Control
  • DOI: 10.2118/128066-MS

The effect of amino acids on ammonium utilization and regeneration by heterotrophic bacteria in the subarctic Pacific
journal, November 1989

  • Kirchman, David L.; Keil, Richard G.; Wheeler, Patricia A.
  • Deep Sea Research Part A. Oceanographic Research Papers, Vol. 36, Issue 11
  • DOI: 10.1016/0198-0149(89)90071-X

Biocides in Hydraulic Fracturing Fluids: A Critical Review of Their Usage, Mobility, Degradation, and Toxicity
journal, December 2014

  • Kahrilas, Genevieve A.; Blotevogel, Jens; Stewart, Philip S.
  • Environmental Science & Technology, Vol. 49, Issue 1
  • DOI: 10.1021/es503724k

Modularity of methylotrophy, revisited: Modularity of methylotrophy, revisited
journal, March 2011

Geochemical evaluation of flowback brine from Marcellus gas wells in Pennsylvania, USA
journal, January 2013

Water Management Challenges Associated with the Production of Shale Gas by Hydraulic Fracturing
journal, June 2011

Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
journal, November 2014

Natural Gas Plays in the Marcellus Shale: Challenges and Potential Opportunities
journal, August 2010

  • Kargbo, David M.; Wilhelm, Ron G.; Campbell, David J.
  • Environmental Science & Technology, Vol. 44, Issue 15, p. 5679-5684
  • DOI: 10.1021/es903811p

The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs: perspectives and prospects
journal, June 2003

Natural fractures in some US shales and their importance for gas production
journal, January 2010

  • Gale, Julia F. W.; Holder, Jon
  • Geological Society, London, Petroleum Geology Conference series, Vol. 7, Issue 1
  • DOI: 10.1144/0071131

Effect of sodium chloride on growth and methane production of methanogens
journal, July 1977

  • Patel, G. B.; Roth, L. A.
  • Canadian Journal of Microbiology, Vol. 23, Issue 7
  • DOI: 10.1139/m77-131

Nitrogen and carbon partitioning in diagenetic and hydrothermal minerals from Paleozoic Black Shales, (Selwyn Basin, Yukon Territories, Canada)
journal, May 2005

Pore‐size constraints on the activity and survival of subsurface bacteria in a late cretaceous shale‐sandstone sequence, northwestern New Mexico
journal, July 1997

  • Fredrickson, J. K.; McKinley, J. P.; Bjornstad, B. N.
  • Geomicrobiology Journal, Vol. 14, Issue 3
  • DOI: 10.1080/01490459709378043

Carboxydocella sporoproducens sp. nov., a novel anaerobic CO-utilizing/H2-producing thermophilic bacterium from a Kamchatka hot spring
journal, April 2006

  • Slepova, T. V.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 56, Issue 4
  • DOI: 10.1099/ijs.0.63961-0

NIH Image to ImageJ: 25 years of image analysis
journal, June 2012

  • Schneider, Caroline A.; Rasband, Wayne S.; Eliceiri, Kevin W.
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2089

Salinity Constraints on Subsurface Archaeal Diversity and Methanogenesis in Sedimentary Rock Rich in Organic Matter
journal, April 2007

  • Waldron, P. J.; Petsch, S. T.; Martini, A. M.
  • Applied and Environmental Microbiology, Vol. 73, Issue 13
  • DOI: 10.1128/AEM.02810-06

Temporal Changes in Microbial Ecology and Geochemistry in Produced Water from Hydraulically Fractured Marcellus Shale Gas Wells
journal, May 2014

  • Cluff, Maryam A.; Hartsock, Angela; MacRae, Jean D.
  • Environmental Science & Technology, Vol. 48, Issue 11
  • DOI: 10.1021/es501173p

Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales
journal, September 2016

Deep subsurface microbial processes
journal, January 1995

  • Lovley, Derek R.; Chapelle, Francis H.
  • Reviews of Geophysics, Vol. 33, Issue 3
  • DOI: 10.1029/95RG01305

Carboxydocella thermautotrophica gen. nov., sp. nov., a novel anaerobic, CO-utilizing thermophile from a Kamchatkan hot spring.
journal, November 2002

  • Sokolova, T. G.; Kostrikina, N. A.; Chernyh, N. A.
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 6
  • DOI: 10.1099/00207713-52-6-1961

Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
journal, May 2012

  • Davis, James P.; Struchtemeyer, Christopher G.; Elshahed, Mostafa S.
  • Microbial Ecology, Vol. 64, Issue 4
  • DOI: 10.1007/s00248-012-0073-3

Can We Treat Hydraulic Fracturing Flowback with a Conventional Biological Process? The Case of Guar Gum
journal, August 2013

  • Lester, Yaal; Yacob, Tesfayohanes; Morrissey, Ian
  • Environmental Science & Technology Letters, Vol. 1, Issue 1
  • DOI: 10.1021/ez4000115

Shale gas development impacts on surface water quality in Pennsylvania
journal, March 2013

  • Olmstead, S. M.; Muehlenbachs, L. A.; Shih, J. -S.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 13
  • DOI: 10.1073/pnas.1213871110

Hot-Alkaline DNA Extraction Method for Deep-Subseafloor Archaeal Communities
journal, January 2014

  • Morono, Yuki; Terada, Takeshi; Hoshino, Tatsuhiko
  • Applied and Environmental Microbiology, Vol. 80, Issue 6
  • DOI: 10.1128/aem.04150-13

Water Use for Shale-Gas Production in Texas, U.S.
journal, March 2012

  • Nicot, Jean-Philippe; Scanlon, Bridget R.
  • Environmental Science & Technology, Vol. 46, Issue 6
  • DOI: 10.1021/es204602t

Characterization of Microbial Diversity in Treated and Untreated Flowback Water Impoundments From Gas Fracturing Operations
conference, April 2013

  • Murali Mohan, Arvind; Gregory, Kelvin; Vidic, Radisav
  • SPE Annual Technical Conference and Exhibition
  • DOI: 10.2118/147414-MS

Microorganisms from deep, high temperature sandstones: constraints on microbial colonization
journal, July 1997

Extremophilic microorganisms: Biochemical adaptation and biotechnological application (review)
journal, January 2010

  • Morozkina, E. V.; Slutskaya, E. S.; Fedorova, T. V.
  • Applied Biochemistry and Microbiology, Vol. 46, Issue 1
  • DOI: 10.1134/S0003683810010011

Microbial Community Changes in Hydraulic Fracturing Fluids and Produced Water from Shale Gas Extraction
journal, October 2013

  • Murali Mohan, Arvind; Hartsock, Angela; Bibby, Kyle J.
  • Environmental Science & Technology, Vol. 47, Issue 22
  • DOI: 10.1021/es402928b

Carbon and nitrogen assimilation in deep subseafloor microbial cells
journal, October 2011

  • Morono, Y.; Terada, T.; Nishizawa, M.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 45
  • DOI: 10.1073/pnas.1107763108

Shale gas development impacts on surface water quality in Pennsylvania
journal, March 2013

  • Olmstead, S. M.; Muehlenbachs, L. A.; Shih, J. -S.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 13
  • DOI: 10.1073/pnas.1213871110

Redox zonation: Equilibrium constraints on the Fe(III)/SO4-reduction interface
journal, September 1996

Geochemical evidence for possible natural migration of Marcellus Formation brine to shallow aquifers in Pennsylvania
journal, July 2012

  • Warner, Nathaniel R.; Jackson, Robert B.; Darrah, Thomas H.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 30
  • DOI: 10.1073/pnas.1121181109

Fiji: an open-source platform for biological-image analysis
journal, June 2012

  • Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2019

Desulfotomaculum thermocisternum sp. nov., a Sulfate Reducer Isolated from a Hot North Sea Oil Reservoir
journal, April 1996

  • Nilsen, R. K.; Torsvik, T.; Lien, T.
  • International Journal of Systematic Bacteriology, Vol. 46, Issue 2
  • DOI: 10.1099/00207713-46-2-397

Generation, transport, and disposal of wastewater associated with Marcellus Shale gas development: RAPID COMMUNICATION
journal, February 2013

  • Lutz, Brian D.; Lewis, Aurana N.; Doyle, Martin W.
  • Water Resources Research, Vol. 49, Issue 2
  • DOI: 10.1002/wrcr.20096

Are Uncultivated Bacteria Really Uncultivable?
journal, January 2012

  • Dewi Puspita, Indun; Kamagata, Yoichi; Tanaka, Michiko
  • Microbes and Environments, Vol. 27, Issue 4
  • DOI: 10.1264/jsme2.me12092

Organic and inorganic composition and microbiology of produced waters from Pennsylvania shale gas wells
journal, September 2015

Carboxydocella thermautotrophica gen. nov., sp. nov., a novel anaerobic, CO-utilizing thermophile from a Kamchatkan hot spring.
journal, November 2002

  • Sokolova, T. G.; Kostrikina, N. A.; Chernyh, N. A.
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 6
  • DOI: 10.1099/00207713-52-6-1961

Living microbial ecosystems within the active zone of catagenesis: Implications for feeding the deep biosphere
journal, June 2006

Microbial communities in flowback water impoundments from hydraulic fracturing for recovery of shale gas
journal, August 2013

  • Murali Mohan, Arvind; Hartsock, Angela; Hammack, Richard W.
  • FEMS Microbiology Ecology, Vol. 86, Issue 3
  • DOI: 10.1111/1574-6941.12183

Revisiting the glyoxylate cycle: alternate pathways for microbial acetate assimilation
journal, July 2006

Biocides in Hydraulic Fracturing Fluids: A Critical Review of Their Usage, Mobility, Degradation, and Toxicity
journal, December 2014

  • Kahrilas, Genevieve A.; Blotevogel, Jens; Stewart, Philip S.
  • Environmental Science & Technology, Vol. 49, Issue 1
  • DOI: 10.1021/es503724k

1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum
journal, April 2012

Identification and activity of acetate-assimilating bacteria in diffuse fluids venting from two deep-sea hydrothermal systems
journal, October 2014

  • Winkel, Matthias; Pjevac, Petra; Kleiner, Manuel
  • FEMS Microbiology Ecology, Vol. 90, Issue 3
  • DOI: 10.1111/1574-6941.12429

Metabolic Capability of a Predominant Halanaerobium sp. in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
journal, June 2016

  • Liang, Renxing; Davidova, Irene A.; Marks, Christopher R.
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00988

Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well
journal, July 1999

  • Ravot, G.; Magot, M.; Fardeau, M. -L.
  • International Journal of Systematic Bacteriology, Vol. 49, Issue 3
  • DOI: 10.1099/00207713-49-3-1141

Diversity of culturable sulfidogenic bacteria in two oil–water separation tanks in the north-eastern oil fields of India
journal, February 2010

The Uncultured Microbial Majority
journal, October 2003

Physical, chemical, and biological characteristics of compounds used in hydraulic fracturing
journal, June 2014

Wastewater Management and Marcellus Shale Gas Development: Trends, Drivers, and Planning Implications
journal, January 2012

  • Rahm, Brian G.; Bates, Josephine; Bertoia, Lara R.
  • SSRN Electronic Journal
  • DOI: 10.2139/ssrn.2176530

Fusibacter bizertensis sp. nov., isolated from a corroded kerosene storage tank
journal, October 2014

  • Smii, L.; Ben Hania, W.; Cayol, J. -L.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 65, Issue Pt 1
  • DOI: 10.1099/ijs.0.066183-0

Spatial and Temporal Correlation of Water Quality Parameters of Produced Waters from Devonian-Age Shale following Hydraulic Fracturing
journal, February 2013

  • Barbot, Elise; Vidic, Natasa S.; Gregory, Kelvin B.
  • Environmental Science & Technology, Vol. 47, Issue 6
  • DOI: 10.1021/es304638h

Anaerophaga thermohalophila gen. nov., sp. nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium.
journal, January 2002

  • Denger, Karin; Ludwig, Wolfgang; Warthmann, Rolf
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 1
  • DOI: 10.1099/00207713-52-1-173

Microbial activity in the marine deep biosphere: progress and prospects
journal, January 2013

  • Orcutt, Beth N.; LaRowe, Douglas E.; Biddle, Jennifer F.
  • Frontiers in Microbiology, Vol. 4
  • DOI: 10.3389/fmicb.2013.00189

Influence of the Drilling Mud Formulation Process on the Bacterial Communities in Thermogenic Natural Gas Wells of the Barnett Shale
journal, May 2011

  • Struchtemeyer, Christopher G.; Davis, James P.; Elshahed, Mostafa S.
  • Applied and Environmental Microbiology, Vol. 77, Issue 14
  • DOI: 10.1128/aem.00233-11

An Energy Balance Concept for Habitability
journal, December 2007

Nitrogen and carbon partitioning in diagenetic and hydrothermal minerals from Paleozoic Black Shales, (Selwyn Basin, Yukon Territories, Canada)
journal, May 2005

Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
journal, May 2012

  • Davis, James P.; Struchtemeyer, Christopher G.; Elshahed, Mostafa S.
  • Microbial Ecology, Vol. 64, Issue 4
  • DOI: 10.1007/s00248-012-0073-3

Natural fractures in some US shales and their importance for gas production
journal, January 2010

  • Gale, Julia F. W.; Holder, Jon
  • Geological Society, London, Petroleum Geology Conference series, Vol. 7, Issue 1
  • DOI: 10.1144/0071131

Microbial communities in flowback water impoundments from hydraulic fracturing for recovery of shale gas
journal, August 2013

  • Murali Mohan, Arvind; Hartsock, Angela; Hammack, Richard W.
  • FEMS Microbiology Ecology, Vol. 86, Issue 3
  • DOI: 10.1111/1574-6941.12183

Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales
journal, September 2016

Fusibacter paucivorans gen. nov., sp. nov., an anaerobic, thiosulfate-reducing bacterium from an oil-producing well
journal, July 1999

  • Ravot, G.; Magot, M.; Fardeau, M. -L.
  • International Journal of Systematic Bacteriology, Vol. 49, Issue 3
  • DOI: 10.1099/00207713-49-3-1141

Biodegradation in Waters from Hydraulic Fracturing: Chemistry, Microbiology, and Engineering
journal, May 2014

The Transport of Carbohydrates by a Bacterial Phosphotransferase System
journal, July 1969

Methanohalophilus zhilinae sp. nov., an Alkaliphilic, Halophilic, Methylotrophic Methanogen
journal, April 1988

  • Mathrani, I. M.; Boone, D. R.; Mah, R. A.
  • International Journal of Systematic Bacteriology, Vol. 38, Issue 2
  • DOI: 10.1099/00207713-38-2-139

Fusibacter tunisiensis sp. nov., isolated from an anaerobic reactor used to treat olive-mill wastewater
journal, August 2011

  • Ben Hania, W.; Fraj, B.; Postec, A.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 6
  • DOI: 10.1099/ijs.0.034603-0

Segmentation of Multi-Isotope Imaging Mass Spectrometry Data for Semi-Automatic Detection of Regions of Interest
journal, February 2012

Geochemical evaluation of flowback brine from Marcellus gas wells in Pennsylvania, USA
journal, January 2013

Metabolic Capability of a Predominant Halanaerobium sp. in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
journal, June 2016

  • Liang, Renxing; Davidova, Irene A.; Marks, Christopher R.
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00988

1,3-Propanediol production and tolerance of a halophilic fermentative bacterium, Halanaerobium saccharolyticum subsp. saccharolyticum
journal, April 2012

Predominance and Metabolic Potential of Halanaerobium spp. in Produced Water from Hydraulically Fractured Marcellus Shale Wells
journal, February 2017

  • Lipus, Daniel; Vikram, Amit; Ross, Daniel
  • Applied and Environmental Microbiology, Vol. 83, Issue 8
  • DOI: 10.1128/AEM.02659-16

Living microbial ecosystems within the active zone of catagenesis: Implications for feeding the deep biosphere
journal, June 2006

Automatic Slide-Loader Fluorescence Microscope for Discriminative Enumeration of Subseafloor Life
journal, January 2010

Coalbed- and Shale-Gas Reservoirs
journal, February 2008

  • Jenkins, Creties D.; Boyer, Charles M.
  • Journal of Petroleum Technology, Vol. 60, Issue 02
  • DOI: 10.2118/103514-JPT

Mechanisms of biofilm resistance to antimicrobial agents
journal, January 2001

Can We Treat Hydraulic Fracturing Flowback with a Conventional Biological Process? The Case of Guar Gum
journal, August 2013

  • Lester, Yaal; Yacob, Tesfayohanes; Morrissey, Ian
  • Environmental Science & Technology Letters, Vol. 1, Issue 1
  • DOI: 10.1021/ez4000115

The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

A critical assessment of the efficacy of biocides used during the hydraulic fracturing process in shale natural gas wells
journal, July 2012

  • Struchtemeyer, Christopher G.; Morrison, Michael D.; Elshahed, Mostafa S.
  • International Biodeterioration & Biodegradation, Vol. 71
  • DOI: 10.1016/j.ibiod.2012.01.013

Microorganisms from deep, high temperature sandstones: constraints on microbial colonization
journal, July 1997

Redox zonation: Equilibrium constraints on the Fe(III)/SO4-reduction interface
journal, September 1996

Drilling accurate nanopores for biosensors by energetic multi-wall carbon nanotubes: a molecular dynamics investigation
journal, September 2022

Sulfate-reducing Bacteria and Their Activities in Oil Production
journal, January 1987

  • Cord-Ruwisch, R.; Kleinitz, W.; Widdel, F.
  • Journal of Petroleum Technology, Vol. 39, Issue 01
  • DOI: 10.2118/13554-PA

Bacterial communities associated with hydraulic fracturing fluids in thermogenic natural gas wells in North Central Texas, USA
journal, November 2011

Coupled laboratory and field investigations resolve microbial interactions that underpin persistence in hydraulically fractured shales
journal, June 2018

  • Borton, Mikayla A.; Hoyt, David W.; Roux, Simon
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 28
  • DOI: 10.1073/pnas.1800155115

Marcellus Shale Post-Frac Flowback Waters - Where is All the Salt Coming from and What are the Implications?
conference, April 2013

  • Blauch, Matthew Eric; Myers, Roger R.; Moore, Tom
  • SPE Eastern Regional Meeting
  • DOI: 10.2118/125740-MS

Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
journal, October 2009

  • Schloss, P. D.; Westcott, S. L.; Ryabin, T.
  • Applied and Environmental Microbiology, Vol. 75, Issue 23, p. 7537-7541
  • DOI: 10.1128/aem.01541-09

Microbial Community Changes in Hydraulic Fracturing Fluids and Produced Water from Shale Gas Extraction
journal, October 2013

  • Murali Mohan, Arvind; Hartsock, Angela; Bibby, Kyle J.
  • Environmental Science & Technology, Vol. 47, Issue 22
  • DOI: 10.1021/es402928b

Microbial production of surfactants and their commercial potential
journal, March 1997

Pore‐size constraints on the activity and survival of subsurface bacteria in a late cretaceous shale‐sandstone sequence, northwestern New Mexico
journal, July 1997

  • Fredrickson, J. K.; McKinley, J. P.; Bjornstad, B. N.
  • Geomicrobiology Journal, Vol. 14, Issue 3
  • DOI: 10.1080/01490459709378043

Flexistipes sinusarabici, a novel genus and species of eubacteria occurring in the Atlantis II Deep brines of the Red Sea
journal, July 1990

  • Fiala, Gerhard; Woese, Carl R.; Langworthy, Thomas A.
  • Archives of Microbiology, Vol. 154, Issue 2
  • DOI: 10.1007/bf00423320

The Uncultured Microbial Majority
journal, October 2003

Coupled laboratory and field investigations resolve microbial interactions that underpin persistence in hydraulically fractured shales
journal, June 2018

  • Borton, Mikayla A.; Hoyt, David W.; Roux, Simon
  • Proceedings of the National Academy of Sciences, Vol. 115, Issue 28
  • DOI: 10.1073/pnas.1800155115

Segmentation of Multi-Isotope Imaging Mass Spectrometry Data for Semi-Automatic Detection of Regions of Interest
journal, February 2012

Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales
journal, July 2017

Diversity of culturable sulfidogenic bacteria in two oil–water separation tanks in the north-eastern oil fields of India
journal, February 2010

Water Use for Shale-Gas Production in Texas, U.S.
journal, March 2012

  • Nicot, Jean-Philippe; Scanlon, Bridget R.
  • Environmental Science & Technology, Vol. 46, Issue 6
  • DOI: 10.1021/es204602t

Desulfotomaculum thermocisternum sp. nov., a Sulfate Reducer Isolated from a Hot North Sea Oil Reservoir
journal, April 1996

  • Nilsen, R. K.; Torsvik, T.; Lien, T.
  • International Journal of Systematic Bacteriology, Vol. 46, Issue 2
  • DOI: 10.1099/00207713-46-2-397

Identification and activity of acetate-assimilating bacteria in diffuse fluids venting from two deep-sea hydrothermal systems
journal, October 2014

  • Winkel, Matthias; Pjevac, Petra; Kleiner, Manuel
  • FEMS Microbiology Ecology, Vol. 90, Issue 3
  • DOI: 10.1111/1574-6941.12429

Haloanaerobium lacusroseus sp. nov., an Extremely Halophilic Fermentative Bacterium from the Sediments of a Hypersaline Lake
journal, October 1995

  • Cayol, J. -L.; Ollivier, B.; Patel, B. K. C.
  • International Journal of Systematic Bacteriology, Vol. 45, Issue 4
  • DOI: 10.1099/00207713-45-4-790

Automatic Slide-Loader Fluorescence Microscope for Discriminative Enumeration of Subseafloor Life
journal, January 2010

Methanohalophilus zhilinae sp. nov., an Alkaliphilic, Halophilic, Methylotrophic Methanogen
journal, April 1988

  • Mathrani, I. M.; Boone, D. R.; Mah, R. A.
  • International Journal of Systematic Bacteriology, Vol. 38, Issue 2
  • DOI: 10.1099/00207713-38-2-139

Organic and inorganic composition and microbiology of produced waters from Pennsylvania shale gas wells
journal, September 2015

Fusibacter tunisiensis sp. nov., isolated from an anaerobic reactor used to treat olive-mill wastewater
journal, August 2011

  • Ben Hania, W.; Fraj, B.; Postec, A.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 62, Issue Pt 6
  • DOI: 10.1099/ijs.0.034603-0

Characterization of hydraulic fracturing flowback water in Colorado: Implications for water treatment
journal, April 2015

Haloanaerobium lacusroseus sp. nov., an Extremely Halophilic Fermentative Bacterium from the Sediments of a Hypersaline Lake
journal, April 1996

  • Cayol, J. -L.; Ollivier, B.; Patel, B. K. C.
  • International Journal of Systematic Bacteriology, Vol. 46, Issue 2
  • DOI: 10.1099/00207713-46-2-628

Corrosion of Iron by Sulfate-Reducing Bacteria: New Views of an Old Problem
journal, December 2013

  • Enning, Dennis; Garrelfs, Julia
  • Applied and Environmental Microbiology, Vol. 80, Issue 4
  • DOI: 10.1128/aem.02848-13

Geochemical evidence for possible natural migration of Marcellus Formation brine to shallow aquifers in Pennsylvania
journal, July 2012

  • Warner, Nathaniel R.; Jackson, Robert B.; Darrah, Thomas H.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 30
  • DOI: 10.1073/pnas.1121181109

Water Management Challenges Associated with the Production of Shale Gas by Hydraulic Fracturing
journal, June 2011

Carbon and nitrogen assimilation in deep subseafloor microbial cells
journal, October 2011

  • Morono, Y.; Terada, T.; Nishizawa, M.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 45
  • DOI: 10.1073/pnas.1107763108

Microbial production of surfactants and their commercial potential.
journal, January 1997

  • Desai, J. D.; Banat, I. M.
  • Microbiology and molecular biology reviews : MMBR, Vol. 61, Issue 1
  • DOI: 10.1128/.61.1.47-64.1997
    Sort by:
    [ × clear filter / sort ]

    Figures / Tables found in this record:

    FIGURE 1 (p. 4)figure
    TABLE 1 (p. 5)table
    FIGURE 2 (p. 6)figure
    FIGURE 3 (p. 6)figure
    FIGURE 4 (p. 7)figure
    FIGURE 5 (p. 8)figure
    TABLE 2 (p. 8)table
      [ × clear filter / sort ]
      Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

      Similar Records in DOE PAGES and OSTI.GOV collections: