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Title: Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities

Abstract

The Caspian Sea, which is the largest landlocked body of water on the planet, receives substantial annual hydrocarbon input from anthropogenic sources (e.g., industry, agriculture, oil exploration, and extraction) and natural sources (e.g., mud volcanoes and oil seeps). The Caspian Sea also receives substantial amounts of runoff from agricultural and municipal sources, containing nutrients that have caused eutrophication and subsequent hypoxia in the deep, cold waters. The effect of decreasing oxygen saturation and cold temperatures on oil hydrocarbon biodegradation by a microbial community is not well characterized. The purpose of this study was to investigate the effect of oxic and anoxic conditions on oil hydrocarbon biodegradation at cold temperatures by microbial communities derived from the Caspian Sea. Water samples were collected from the Caspian Sea for study in experimental microcosms. Major taxonomic orders observed in the ambient water samples included Flavobacteriales, Actinomycetales, and Oceanospirillales. Microcosms were inoculated with microbial communities from the deepest waters and amended with oil hydrocarbons for 17 days. Hydrocarbon degradation and shifts in microbial community structure were measured. Surprisingly, oil hydrocarbon biodegradation under anoxic conditions exceeded that under oxic conditions; this was particularly evident in the degradation of aromatic hydrocarbons. Important microbial taxa associated with themore » anoxic microcosms included known oil degraders such as Oceanospirillaceae. This study provides knowledge about the ambient community structure of the Caspian Sea, which serves as an important reference point for future studies. Furthermore, this may be the first report in which anaerobic biodegradation of oil hydrocarbons exceeds aerobic biodegradation.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [5];  [6];  [7];  [8];  [8];  [9];  [9]; ORCiD logo [1]
  1. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Michigan Technological Univ., Houghton, MI (United States)
  3. Stanford Univ., CA (United States)
  4. McGill Univ., Montreal, QC (Canada)
  5. Univ. of Tennessee, Knoxville, TN (United States)
  6. Harvard School of Public Health, Cambridge, MA (United States)
  7. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
  8. Florida International University, Miami, FL (United States)
  9. BP, Baku (Azerbaijan)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1528693
Alternate Identifier(s):
OSTI ID: 1561904
Grant/Contract Number:  
AC05-00OR22725; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Volume: 10; Journal Issue: n/a; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 16S rRNA gene sequencing; Caspian Sea; anaerobic; cold temperature; marine microbial community; microbial ecology; oil biodegradation; petroleum

Citation Formats

Miller, John I., Techtmann, Stephen, Fortney, Julian, Mahmoudi, Nagissa, Joyner, Dominique, Liu, Jiang, Olesen, Scott, Alm, Eric, Fernandez, Adolfo, Gardinali, Piero, GaraJayeva, Nargiz, Askerov, Faig S., and Hazen, Terry C. Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities. United States: N. p., 2019. Web. doi:10.3389/fmicb.2019.00995.
Miller, John I., Techtmann, Stephen, Fortney, Julian, Mahmoudi, Nagissa, Joyner, Dominique, Liu, Jiang, Olesen, Scott, Alm, Eric, Fernandez, Adolfo, Gardinali, Piero, GaraJayeva, Nargiz, Askerov, Faig S., & Hazen, Terry C. Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities. United States. doi:10.3389/fmicb.2019.00995.
Miller, John I., Techtmann, Stephen, Fortney, Julian, Mahmoudi, Nagissa, Joyner, Dominique, Liu, Jiang, Olesen, Scott, Alm, Eric, Fernandez, Adolfo, Gardinali, Piero, GaraJayeva, Nargiz, Askerov, Faig S., and Hazen, Terry C. Thu . "Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities". United States. doi:10.3389/fmicb.2019.00995. https://www.osti.gov/servlets/purl/1528693.
@article{osti_1528693,
title = {Oil Hydrocarbon Degradation by Caspian Sea Microbial Communities},
author = {Miller, John I. and Techtmann, Stephen and Fortney, Julian and Mahmoudi, Nagissa and Joyner, Dominique and Liu, Jiang and Olesen, Scott and Alm, Eric and Fernandez, Adolfo and Gardinali, Piero and GaraJayeva, Nargiz and Askerov, Faig S. and Hazen, Terry C.},
abstractNote = {The Caspian Sea, which is the largest landlocked body of water on the planet, receives substantial annual hydrocarbon input from anthropogenic sources (e.g., industry, agriculture, oil exploration, and extraction) and natural sources (e.g., mud volcanoes and oil seeps). The Caspian Sea also receives substantial amounts of runoff from agricultural and municipal sources, containing nutrients that have caused eutrophication and subsequent hypoxia in the deep, cold waters. The effect of decreasing oxygen saturation and cold temperatures on oil hydrocarbon biodegradation by a microbial community is not well characterized. The purpose of this study was to investigate the effect of oxic and anoxic conditions on oil hydrocarbon biodegradation at cold temperatures by microbial communities derived from the Caspian Sea. Water samples were collected from the Caspian Sea for study in experimental microcosms. Major taxonomic orders observed in the ambient water samples included Flavobacteriales, Actinomycetales, and Oceanospirillales. Microcosms were inoculated with microbial communities from the deepest waters and amended with oil hydrocarbons for 17 days. Hydrocarbon degradation and shifts in microbial community structure were measured. Surprisingly, oil hydrocarbon biodegradation under anoxic conditions exceeded that under oxic conditions; this was particularly evident in the degradation of aromatic hydrocarbons. Important microbial taxa associated with the anoxic microcosms included known oil degraders such as Oceanospirillaceae. This study provides knowledge about the ambient community structure of the Caspian Sea, which serves as an important reference point for future studies. Furthermore, this may be the first report in which anaerobic biodegradation of oil hydrocarbons exceeds aerobic biodegradation.},
doi = {10.3389/fmicb.2019.00995},
journal = {Frontiers in Microbiology},
number = n/a,
volume = 10,
place = {United States},
year = {2019},
month = {5}
}

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