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Title: TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana

Abstract

Summary O ‐Acetylated pectins are abundant in the primary cell wall of plants and growing evidence suggests they have important roles in plant cell growth and interaction with the environment. Despite their importance, genes required for O ‐acetylation of pectins are still largely unknown. In this study, we showed that TRICHOME BIREFRINGENCE LIKE 10 ( AT 3G06080 ) is involved in O ‐acetylation of pectins in Arabidopsis ( Arabidopsis thaliana ). The activity of the TBL 10 promoter was strong in tissues where pectins are highly abundant ( e.g . leaves). Two homozygous knock‐out mutants of Arabidopsis, tbl10‐1 and tbl10‐2 , were isolated and shown to exhibit reduced levels of wall‐bound acetyl esters, equivalent of ~50% of the wild‐type level in pectin‐enriched fractions derived from leaves. Further fractionation revealed that the degree of acetylation of the pectin rhamnogalacturonan‐I ( RG ‐I) was reduced in the tbl10 mutant compared to the wild type, whereas the pectin homogalacturonan ( HG ) was unaffected. The degrees of acetylation in hemicelluloses ( i.e . xyloglucan, xylan and mannan) were indistinguishable between the tbl10 mutants and the wild type. The mutant plants contained normal trichomes in leaves and exhibited a similar level of susceptibility tomore » the phytopathogenic microorganisms Pseudomonas syringae pv. tomato DC 3000 and Botrytis cinerea ; while they displayed enhanced tolerance to drought. These results indicate that TBL 10 is required for O ‐acetylation of RG ‐I, possibly as an acetyltransferase, and suggest that O ‐acetylated RG ‐I plays a role in abiotic stress responses in Arabidopsis.« less

Authors:
 [1];  [2];  [1];  [2];  [3];  [4];  [4];  [5];  [5]; ORCiD logo [6]; ORCiD logo [1]
+ Show Author Affiliations
  1. Univ. of Copenhagen (Denmark)
  2. Joint Bioenergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Joint Bioenergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Nanjing Agricultural Univ., Nanjing, Jiangsu (China)
  4. Normandie Univ., Rouen (France)
  5. RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa (Japan)
  6. Joint Bioenergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Joint BioEnergy Inst.; China Scholarship Council; Villum Foundation; Innovation Foundation Denmark
OSTI Identifier:
1515776
Alternate Identifier(s):
OSTI ID: 1474785
Grant/Contract Number:  
AC02-05CH11231; 001‐2011‐4; 13363
Resource Type:
Accepted Manuscript
Journal Name:
The Plant Journal
Additional Journal Information:
Journal Volume: 96; Journal Issue: 4; Journal ID: ISSN 0960-7412
Publisher:
Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; O‐acetylation; pectin; rhamnogalacturonan I; the cell wall; Arabidopsis thaliana

Citation Formats

Stranne, Maria, Ren, Yanfang, Fimognari, Lorenzo, Birdseye, Devon, Yan, Jingwei, Bardor, Muriel, Mollet, Jean-Claude, Komatsu, Takanori, Kikuchi, Jun, Scheller, Henrik V., and Sakuragi, Yumiko. TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana. United States: N. p., 2018. Web. doi:10.1111/tpj.14067.
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Stranne, Maria, Ren, Yanfang, Fimognari, Lorenzo, Birdseye, Devon, Yan, Jingwei, Bardor, Muriel, Mollet, Jean-Claude, Komatsu, Takanori, Kikuchi, Jun, Scheller, Henrik V., & Sakuragi, Yumiko. TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana. United States. https://doi.org/10.1111/tpj.14067
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Stranne, Maria, Ren, Yanfang, Fimognari, Lorenzo, Birdseye, Devon, Yan, Jingwei, Bardor, Muriel, Mollet, Jean-Claude, Komatsu, Takanori, Kikuchi, Jun, Scheller, Henrik V., and Sakuragi, Yumiko. Fri . "TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana". United States. https://doi.org/10.1111/tpj.14067. https://www.osti.gov/servlets/purl/1515776.
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@article{osti_1515776,
title = {TBL10 is required for O-acetylation of pectic rhamnogalacturonan-I in Arabidopsis thaliana},
author = {Stranne, Maria and Ren, Yanfang and Fimognari, Lorenzo and Birdseye, Devon and Yan, Jingwei and Bardor, Muriel and Mollet, Jean-Claude and Komatsu, Takanori and Kikuchi, Jun and Scheller, Henrik V. and Sakuragi, Yumiko},
abstractNote = {Summary O ‐Acetylated pectins are abundant in the primary cell wall of plants and growing evidence suggests they have important roles in plant cell growth and interaction with the environment. Despite their importance, genes required for O ‐acetylation of pectins are still largely unknown. In this study, we showed that TRICHOME BIREFRINGENCE LIKE 10 ( AT 3G06080 ) is involved in O ‐acetylation of pectins in Arabidopsis ( Arabidopsis thaliana ). The activity of the TBL 10 promoter was strong in tissues where pectins are highly abundant ( e.g . leaves). Two homozygous knock‐out mutants of Arabidopsis, tbl10‐1 and tbl10‐2 , were isolated and shown to exhibit reduced levels of wall‐bound acetyl esters, equivalent of ~50% of the wild‐type level in pectin‐enriched fractions derived from leaves. Further fractionation revealed that the degree of acetylation of the pectin rhamnogalacturonan‐I ( RG ‐I) was reduced in the tbl10 mutant compared to the wild type, whereas the pectin homogalacturonan ( HG ) was unaffected. The degrees of acetylation in hemicelluloses ( i.e . xyloglucan, xylan and mannan) were indistinguishable between the tbl10 mutants and the wild type. The mutant plants contained normal trichomes in leaves and exhibited a similar level of susceptibility to the phytopathogenic microorganisms Pseudomonas syringae pv. tomato DC 3000 and Botrytis cinerea ; while they displayed enhanced tolerance to drought. These results indicate that TBL 10 is required for O ‐acetylation of RG ‐I, possibly as an acetyltransferase, and suggest that O ‐acetylated RG ‐I plays a role in abiotic stress responses in Arabidopsis.},
doi = {10.1111/tpj.14067},
journal = {The Plant Journal},
number = 4,
volume = 96,
place = {United States},
year = {Fri Aug 17 00:00:00 EDT 2018},
month = {Fri Aug 17 00:00:00 EDT 2018}
}
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https://doi.org/10.1111/tpj.14067
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