Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models
Abstract
We are developing a laboratory-scale model to improve our understanding and capacity to assess the biological risks of genetically engineered bacteria and their genetic elements in the natural environment. Our hypothetical scenario concerns an industrial bioreactor failure resulting in the introduction of genetically engineered bacteria to a downstream municipal wastewater treatment plant (MWWTP). As the first step towards developing a model for this scenario, we sampled microbial communities from the aeration basin of a MWWTP at three seasonal time points. Having established a baseline for community composition, we investigated how the community changed when propagated in the laboratory, including cell culture media conditions that could provide selective pressure in future studies. Specifically, using PhyloChip 16S-rRNA-gene targeting microarrays, we compared the compositions of sampled communities to those of inocula propagated in the laboratory in simulated wastewater conditionally amended with various carbon sources (glucose, chloroacetate, D-threonine) or the ionic liquid 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl). Proteobacteria, Bacteroidetes, and Actinobacteria were predominant in both aeration basin and laboratory-cultured communities. Laboratory-cultured communities were enriched in γ-Proteobacteria. Enterobacteriaceae, and Aeromonadaceae were enriched by glucose, Pseudomonadaceae by chloroacetate and D-threonine, and Burkholderiacea by high (50 mM) concentrations of chloroacetate. Microbial communities cultured with chloroacetate and D-threonine were moremore »
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA), Office of Defense Nuclear Nonproliferation
- OSTI Identifier:
- 1511406
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 8; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Lee, Sangwon, Geller, Jil T., Torok, Tamas, Wu, Cindy H., Singer, Mary, Reid, Francine C., Tarjan, Daniel R., Hazen, Terry C., Arkin, Adam P., and Hillson, Nathan J. Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0105689.
Lee, Sangwon, Geller, Jil T., Torok, Tamas, Wu, Cindy H., Singer, Mary, Reid, Francine C., Tarjan, Daniel R., Hazen, Terry C., Arkin, Adam P., & Hillson, Nathan J. Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models. United States. https://doi.org/10.1371/journal.pone.0105689
Lee, Sangwon, Geller, Jil T., Torok, Tamas, Wu, Cindy H., Singer, Mary, Reid, Francine C., Tarjan, Daniel R., Hazen, Terry C., Arkin, Adam P., and Hillson, Nathan J. Fri .
"Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models". United States. https://doi.org/10.1371/journal.pone.0105689. https://www.osti.gov/servlets/purl/1511406.
@article{osti_1511406,
title = {Characterization of Wastewater Treatment Plant Microbial Communities and the Effects of Carbon Sources on Diversity in Laboratory Models},
author = {Lee, Sangwon and Geller, Jil T. and Torok, Tamas and Wu, Cindy H. and Singer, Mary and Reid, Francine C. and Tarjan, Daniel R. and Hazen, Terry C. and Arkin, Adam P. and Hillson, Nathan J.},
abstractNote = {We are developing a laboratory-scale model to improve our understanding and capacity to assess the biological risks of genetically engineered bacteria and their genetic elements in the natural environment. Our hypothetical scenario concerns an industrial bioreactor failure resulting in the introduction of genetically engineered bacteria to a downstream municipal wastewater treatment plant (MWWTP). As the first step towards developing a model for this scenario, we sampled microbial communities from the aeration basin of a MWWTP at three seasonal time points. Having established a baseline for community composition, we investigated how the community changed when propagated in the laboratory, including cell culture media conditions that could provide selective pressure in future studies. Specifically, using PhyloChip 16S-rRNA-gene targeting microarrays, we compared the compositions of sampled communities to those of inocula propagated in the laboratory in simulated wastewater conditionally amended with various carbon sources (glucose, chloroacetate, D-threonine) or the ionic liquid 1-ethyl-3-methylimidazolium chloride ([C2mim]Cl). Proteobacteria, Bacteroidetes, and Actinobacteria were predominant in both aeration basin and laboratory-cultured communities. Laboratory-cultured communities were enriched in γ-Proteobacteria. Enterobacteriaceae, and Aeromonadaceae were enriched by glucose, Pseudomonadaceae by chloroacetate and D-threonine, and Burkholderiacea by high (50 mM) concentrations of chloroacetate. Microbial communities cultured with chloroacetate and D-threonine were more similar to sampled field communities than those cultured with glucose or [C2mim]Cl. Although observed relative richness in operational taxonomic units (OTUs) was lower for laboratory cultures than for field communities, both flask and reactor systems supported phylogenetically diverse communities. These results importantly provide a foundation for laboratory models of industrial bioreactor failure scenarios.},
doi = {10.1371/journal.pone.0105689},
journal = {PLoS ONE},
number = 8,
volume = 9,
place = {United States},
year = {Fri Aug 22 00:00:00 EDT 2014},
month = {Fri Aug 22 00:00:00 EDT 2014}
}
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Cited by: 6 works
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Figures / Tables:
Figure 1: Overview of Experiments. All substrates were tested in triplicate, except for ‘No-Carbon in bioreactors’, which was tested in duplicate. The No-Carbon and [C2mim]Cl triplicates in flasks were pooled in order to provide adequate biomass for PhyloChip analysis.
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