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Title: Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation

Abstract

The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.

Authors:
ORCiD logo [1]; ORCiD logo [1]
  1. Dept. of Biochemistry and Purdue Center for Plant Biology, West Lafayette, IN (United States)
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1510783
Grant/Contract Number:  
FG02-07ER15905
Resource Type:
Accepted Manuscript
Journal Name:
G3
Additional Journal Information:
Journal Volume: 8; Journal Issue: 9; Journal ID: ISSN 2160-1836
Publisher:
Genetics Society of America
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Mediator; Arabidopsis; transcription regulation; gene expression

Citation Formats

Dolan, Whitney L., and Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States: N. p., 2018. Web. doi:10.1534/g3.118.200573.
Dolan, Whitney L., & Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States. doi:10.1534/g3.118.200573.
Dolan, Whitney L., and Chapple, Clint. Thu . "Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation". United States. doi:10.1534/g3.118.200573. https://www.osti.gov/servlets/purl/1510783.
@article{osti_1510783,
title = {Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation},
author = {Dolan, Whitney L. and Chapple, Clint},
abstractNote = {The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.},
doi = {10.1534/g3.118.200573},
journal = {G3},
number = 9,
volume = 8,
place = {United States},
year = {2018},
month = {7}
}

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Works referenced in this record:

Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids
journal, January 2014

  • Greaves, Ian K.; Groszmann, Michael; Wang, Aihua
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 5, p. 2017-2022
  • DOI: 10.1073/pnas.1323656111