Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation
Abstract
The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.
- Authors:
-
[1];
[1]
- Dept. of Biochemistry and Purdue Center for Plant Biology, West Lafayette, IN (United States)
- Publication Date:
- Research Org.:
- Purdue Univ., West Lafayette, IN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1510783
- Grant/Contract Number:
- FG02-07ER15905
- Resource Type:
- Accepted Manuscript
- Journal Name:
- G3
- Additional Journal Information:
- Journal Volume: 8; Journal Issue: 9; Journal ID: ISSN 2160-1836
- Publisher:
- Genetics Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Mediator; Arabidopsis; transcription regulation; gene expression
Citation Formats
Dolan, Whitney L., and Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States: N. p., 2018.
Web. doi:10.1534/g3.118.200573.
Dolan, Whitney L., & Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States. https://doi.org/10.1534/g3.118.200573
Dolan, Whitney L., and Chapple, Clint. Thu .
"Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation". United States. https://doi.org/10.1534/g3.118.200573. https://www.osti.gov/servlets/purl/1510783.
@article{osti_1510783,
title = {Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation},
author = {Dolan, Whitney L. and Chapple, Clint},
abstractNote = {The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.},
doi = {10.1534/g3.118.200573},
journal = {G3},
number = 9,
volume = 8,
place = {United States},
year = {2018},
month = {7}
}
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Small-sample estimation of negative binomial dispersion, with applications to SAGE data
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The TRAP100 component of the TRAP/Mediator complex is essential in broad transcriptional events and development
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The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function
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The Arabidopsis Mediator Complex Subunit16 Is a Key Component of Basal Resistance against the Necrotrophic Fungal Pathogen Sclerotinia sclerotiorum
journal, July 2015
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Conserved Alternative Splicing of Arabidopsis Transthyretin-Like Determines Protein Localization and S -Allantoin Synthesis in Peroxisomes
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The Arabidopsis Mediator Complex Subunit16 Positively Regulates Salicylate-Mediated Systemic Acquired Resistance and Jasmonate/Ethylene-Induced Defense Pathways
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Mediator Complex Subunits MED2, MED5, MED16, and MED23 Genetically Interact in the Regulation of Phenylpropanoid Biosynthesis
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Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
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The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
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In Vivo Requirement of Activator-Specific Binding Targets of Mediator
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A Triad of Subunits from the Gal11/Tail Domain of Srb Mediator Is an In Vivo Target of Transcriptional Activator Gcn4p
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The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana
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Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
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Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units
journal, January 2006
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An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets
journal, August 2007
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The M ediator subunit MED 23 couples H2B mono‐ubiquitination to transcriptional control and cell fate determination
journal, September 2015
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- The EMBO Journal, Vol. 34, Issue 23
Self-assembly of embryonic and two extra-embryonic stem cell types into gastrulating embryo-like structures.
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Role of Arabidopsis MYC and MYB Homologs in Drought- and Abscisic Acid-Regulated Gene Expression
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journal, September 2015
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Plant mediator
journal, June 2010
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Mediator MED23 regulates basal transcription in vivo via an interaction with P-TEFb
journal, January 2013
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Molecular architecture of the yeast Mediator complex
journal, September 2015
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Molecular Architecture Of The Yeast Mediator Complex
dataset, January 2015
- Robinson, Philip J.; Trnka, Michael J.; Pellarin, Riccardo
- Zenodo
Additional file 2: of The Mediator complex subunits MED25/PFT1 and MED8 are required for transcriptional responses to changes in cell wall arabinose composition and glucose treatment in Arabidopsis thaliana
dataset, January 2015
- Seguela-Arnaud, Mathilde; Smith, Caroline; Uribe, Marcos
- Figshare
Works referencing / citing this record:
NeVOmics: An Enrichment Tool for Gene Ontology and Functional Network Analysis and Visualization of Data from OMICs Technologies
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- Genes, Vol. 9, Issue 12