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Title: Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation

Abstract

The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.

Authors:
ORCiD logo [1]; ORCiD logo [1]
  1. Dept. of Biochemistry and Purdue Center for Plant Biology, West Lafayette, IN (United States)
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1510783
Grant/Contract Number:  
FG02-07ER15905
Resource Type:
Accepted Manuscript
Journal Name:
G3
Additional Journal Information:
Journal Volume: 8; Journal Issue: 9; Journal ID: ISSN 2160-1836
Publisher:
Genetics Society of America
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Mediator; Arabidopsis; transcription regulation; gene expression

Citation Formats

Dolan, Whitney L., and Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States: N. p., 2018. Web. doi:10.1534/g3.118.200573.
Dolan, Whitney L., & Chapple, Clint. Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation. United States. doi:10.1534/g3.118.200573.
Dolan, Whitney L., and Chapple, Clint. Thu . "Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation". United States. doi:10.1534/g3.118.200573. https://www.osti.gov/servlets/purl/1510783.
@article{osti_1510783,
title = {Transcriptome Analysis of Four Arabidopsis thaliana Mediator Tail Mutants Reveals Overlapping and Unique Functions in Gene Regulation},
author = {Dolan, Whitney L. and Chapple, Clint},
abstractNote = {The Mediator complex is a central component of transcriptional regulation in Eukaryotes. The complex is structurally divided into four modules known as the head, middle, tail and kinase modules, and in Arabidopsis thaliana, comprises 28-34 subunits. Here, we explore the functions of four Arabidopsis Medi-ator tail subunits, MED2, MED5a/b, MED16, and MED23, by comparing the impact of mutations in each on the Arabidopsis transcriptome. We find that these subunits affect both unique and overlapping sets of genes, providing insight into the functional and structural relationships between them. The mutants pri-marily exhibit changes in the expression of genes related to biotic and abiotic stress. We find evidence for a tissue specific role for MED23, as well as in the production of alternative transcripts. Together, our data help disentangle the individual contributions of these MED subunits to global gene expression and suggest new avenues for future research into their functions.},
doi = {10.1534/g3.118.200573},
journal = {G3},
number = 9,
volume = 8,
place = {United States},
year = {2018},
month = {7}
}

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Cited by: 3 works
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Works referenced in this record:

An Activator Target in the RNA Polymerase II Holoenzyme
journal, May 1998


Mediator structure and rearrangements required for holoenzyme formation
journal, March 2017

  • Tsai, Kuang-Lei; Yu, Xiaodi; Gopalan, Sneha
  • Nature, Vol. 544, Issue 7649
  • DOI: 10.1038/nature21393

In Vivo Requirement of Activator-Specific Binding Targets of Mediator
journal, December 2000


Mediator: A key regulator of plant development
journal, November 2016


CDK8 is a positive regulator of transcriptional elongation within the serum response network
journal, January 2010

  • Donner, Aaron J.; Ebmeier, Christopher C.; Taatjes, Dylan J.
  • Nature Structural & Molecular Biology, Vol. 17, Issue 2
  • DOI: 10.1038/nsmb.1752

Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
journal, September 2011


Complexity of the Alternative Splicing Landscape in Plants
journal, October 2013

  • Reddy, Anireddy S. N.; Marquez, Yamile; Kalyna, Maria
  • The Plant Cell, Vol. 25, Issue 10
  • DOI: 10.1105/tpc.113.117523

A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
journal, July 1993


PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25 Regulates Lateral Root Formation via Auxin Signaling in Arabidopsis
journal, April 2014

  • Raya-González, Javier; Ortiz-Castro, Randy; Ruíz-Herrera, León Francisco
  • Plant Physiology, Vol. 165, Issue 2
  • DOI: 10.1104/pp.114.239806

ImageJ2: ImageJ for the next generation of scientific image data
journal, November 2017

  • Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
  • BMC Bioinformatics, Vol. 18, Issue 1
  • DOI: 10.1186/s12859-017-1934-z

TopHat: discovering splice junctions with RNA-Seq
journal, March 2009


A novel mediator between activator proteins and the RNA polymerase II transcription apparatus
journal, June 1990


An Activator Binding Module of Yeast RNA Polymerase II Holoenzyme
journal, April 1999

  • Lee, Young Chul; Park, Jin Mo; Min, Soyoung
  • Molecular and Cellular Biology, Vol. 19, Issue 4
  • DOI: 10.1128/MCB.19.4.2967

ATTED-II in 2016: A Plant Coexpression Database Towards Lineage-Specific Coexpression
journal, November 2015

  • Aoki, Yuichi; Okamura, Yasunobu; Tadaka, Shu
  • Plant and Cell Physiology, Vol. 57, Issue 1
  • DOI: 10.1093/pcp/pcv165

The Structural and Functional Role of Med5 in the Yeast Mediator Tail Module
journal, October 2005

  • Béve, Jenny; Hu, Guo-Zhen; Myers, Lawrence C.
  • Journal of Biological Chemistry, Vol. 280, Issue 50
  • DOI: 10.1074/jbc.M511181200

Conservation and Divergence of Mediator Structure and Function: Insights from Plants
journal, November 2016


The M ediator subunit MED 23 couples H2B mono‐ubiquitination to transcriptional control and cell fate determination
journal, September 2015


Mediator Expression Profiling Epistasis Reveals a Signal Transduction Pathway with Antagonistic Submodules and Highly Specific Downstream Targets
journal, August 2005


Mediator MED23 regulates basal transcription in vivo via an interaction with P-TEFb
journal, January 2013


Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation: YUCCA4 and auxin biosynthesis
journal, January 2012


Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
journal, November 1995

  • Li, Y.; Bjorklund, S.; Jiang, Y. W.
  • Proceedings of the National Academy of Sciences, Vol. 92, Issue 24
  • DOI: 10.1073/pnas.92.24.10864

The Mediator Complex Subunit PFT1 Is a Key Regulator of Jasmonate-Dependent Defense in Arabidopsis
journal, August 2009

  • Kidd, Brendan N.; Edgar, Cameron I.; Kumar, Krish K.
  • The Plant Cell, Vol. 21, Issue 8
  • DOI: 10.1105/tpc.109.066910

Architecture of the RNA polymerase II–Mediator core initiation complex
journal, February 2015

  • Plaschka, C.; Larivière, L.; Wenzeck, L.
  • Nature, Vol. 518, Issue 7539
  • DOI: 10.1038/nature14229

A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis
journal, March 2013

  • Remy, Estelle; Cabrito, Tânia R.; Baster, Pawel
  • The Plant Cell, Vol. 25, Issue 3
  • DOI: 10.1105/tpc.113.110353

An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets
journal, August 2007


The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk in Arabidopsis
journal, December 2016


Molecular architecture of the yeast Mediator complex
journal, September 2015

  • Robinson, Philip J.; Trnka, Michael J.; Pellarin, Riccardo
  • eLife, Vol. 4
  • DOI: 10.7554/eLife.08719

Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
journal, May 2014

  • Zhang, Yue; Wu, Huilan; Wang, Ning
  • New Phytologist, Vol. 203, Issue 3
  • DOI: 10.1111/nph.12860

The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function
journal, February 2009

  • Knuesel, M. T.; Meyer, K. D.; Bernecky, C.
  • Genes & Development, Vol. 23, Issue 4
  • DOI: 10.1101/gad.1767009

Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants
journal, September 2015


Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
journal, December 2008

  • Huang, Da Wei; Sherman, Brad T.; Lempicki, Richard A.
  • Nature Protocols, Vol. 4, Issue 1
  • DOI: 10.1038/nprot.2008.211

Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids
journal, January 2014

  • Greaves, Ian K.; Groszmann, Michael; Wang, Aihua
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 5, p. 2017-2022
  • DOI: 10.1073/pnas.1323656111

The MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis
journal, December 2009

  • Gillmor, C. S.; Park, M. Y.; Smith, M. R.
  • Development, Vol. 137, Issue 1
  • DOI: 10.1242/dev.043174

A Triad of Subunits from the Gal11/Tail Domain of Srb Mediator Is an In Vivo Target of Transcriptional Activator Gcn4p
journal, July 2004


Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo
journal, November 2016


Small-sample estimation of negative binomial dispersion, with applications to SAGE data
journal, July 2007


Conserved Alternative Splicing of Arabidopsis Transthyretin-Like Determines Protein Localization and S -Allantoin Synthesis in Peroxisomes
journal, May 2010

  • Lamberto, Ilaria; Percudani, Riccardo; Gatti, Rita
  • The Plant Cell, Vol. 22, Issue 5
  • DOI: 10.1105/tpc.109.070102

edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
journal, November 2009


HTSeq--a Python framework to work with high-throughput sequencing data
journal, September 2014


Plant Mediator complex and its critical functions in transcription regulation: Plant Mediator complex and its function
journal, September 2015

  • Yang, Yan; Li, Ling; Qu, Li-Jia
  • Journal of Integrative Plant Biology, Vol. 58, Issue 2
  • DOI: 10.1111/jipb.12377

The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
journal, May 1999

  • Knight, Heather; Veale, Emma L.; Warren, Gareth J.
  • The Plant Cell, Vol. 11, Issue 5
  • DOI: 10.1105/tpc.11.5.875

Regulation of flowering time by light quality
journal, June 2003


Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units
journal, January 2006


A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction
journal, April 2013

  • Tsai, Kuang-Lei; Sato, Shigeo; Tomomori-Sato, Chieri
  • Nature Structural & Molecular Biology, Vol. 20, Issue 5
  • DOI: 10.1038/nsmb.2549

Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit
journal, February 2012


Fiji: an open-source platform for biological-image analysis
journal, June 2012

  • Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2019

Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator
journal, February 2014

  • Gonzalez, D.; Hamidi, N.; Del Sol, R.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 7
  • DOI: 10.1073/pnas.1307525111

MEDIATOR25 Acts as an Integrative Hub for the Regulation of Jasmonate-Responsive Gene Expression in Arabidopsis
journal, July 2012

  • Çevik, Volkan; Kidd, Brendan N.; Zhang, Peijun
  • Plant Physiology, Vol. 160, Issue 1
  • DOI: 10.1104/pp.112.202697

Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
journal, January 2009

  • Dixon, David P.; Hawkins, Timothy; Hussey, Patrick J.
  • Journal of Experimental Botany, Vol. 60, Issue 4
  • DOI: 10.1093/jxb/ern365

Mediator protein mutations that selectively abolish activated transcription
journal, January 1999

  • Myers, L. C.; Gustafsson, C. M.; Hayashibara, K. C.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 1
  • DOI: 10.1073/pnas.96.1.67

SENSITIVE TO FREEZING6 Integrates Cellular and Environmental Inputs to the Plant Circadian Clock
journal, July 2008

  • Knight, Heather; Thomson, Adrian J. W.; McWatters, Harriet G.
  • Plant Physiology, Vol. 148, Issue 1
  • DOI: 10.1104/pp.108.123901

The Arabidopsis Mediator Complex Subunits MED16, MED14, and MED2 Regulate Mediator and RNA Polymerase II Recruitment to CBF-Responsive Cold-Regulated Genes
journal, January 2014

  • Hemsley, Piers A.; Hurst, Charlotte H.; Kaliyadasa, Ewon
  • The Plant Cell, Vol. 26, Issue 1
  • DOI: 10.1105/tpc.113.117796

The Mediator subunit SFR6/MED16 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways
journal, April 2012


The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
journal, October 2006

  • Elmlund, H.; Baraznenok, V.; Lindahl, M.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 43
  • DOI: 10.1073/pnas.0607483103

Facilitated Assembly of the Preinitiation Complex by Separated Tail and Head/Middle Modules of the Mediator
journal, January 2012

  • Galdieri, Luciano; Desai, Parima; Vancura, Ales
  • Journal of Molecular Biology, Vol. 415, Issue 3
  • DOI: 10.1016/j.jmb.2011.11.020

Mediator Complex Subunits MED2, MED5, MED16, and MED23 Genetically Interact in the Regulation of Phenylpropanoid Biosynthesis
journal, December 2017

  • Dolan, Whitney L.; Dilkes, Brian P.; Stout, Jake M.
  • The Plant Cell, Vol. 29, Issue 12
  • DOI: 10.1105/tpc.17.00282

The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress
journal, May 2003


Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit
journal, November 2014

  • Cevher, Murat A.; Shi, Yi; Li, Dan
  • Nature Structural & Molecular Biology, Vol. 21, Issue 12
  • DOI: 10.1038/nsmb.2914

Identification of SFR6, a key component in cold acclimation acting post-translationally on CBF function: SFR6 and cold acclimation
journal, January 2009


The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
journal, May 2011

  • Elfving, N.; Davoine, C.; Benlloch, R.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 20
  • DOI: 10.1073/pnas.1002981108

PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis
journal, October 2012

  • Sundaravelpandian, Kalaipandian; Chandrika, Nulu Naga Prafulla; Schmidt, Wolfgang
  • New Phytologist, Vol. 197, Issue 1
  • DOI: 10.1111/nph.12000

Human Mediator Subunit MED26 Functions as a Docking Site for Transcription Elongation Factors
journal, July 2011


The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
journal, February 2014

  • Yang, Yan; Ou, Bin; Zhang, Jinzhe
  • The Plant Journal, Vol. 77, Issue 6
  • DOI: 10.1111/tpj.12440

REF4 and RFR1, Subunits of the Transcriptional Coregulatory Complex Mediator, Are Required for Phenylpropanoid Homeostasis in Arabidopsis
journal, December 2011

  • Bonawitz, Nicholas D.; Soltau, Whitney L.; Blatchley, Michael R.
  • Journal of Biological Chemistry, Vol. 287, Issue 8
  • DOI: 10.1074/jbc.M111.312298

The plant Mediator and its role in noncoding RNA production
journal, April 2011


FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
journal, May 1999

  • Michaels, Scott D.; Amasino, Richard M.
  • The Plant Cell, Vol. 11, Issue 5
  • DOI: 10.1105/tpc.11.5.949

The Mediator complex and transcription regulation
journal, October 2013

  • Poss, Zachary C.; Ebmeier, Christopher C.; Taatjes, Dylan J.
  • Critical Reviews in Biochemistry and Molecular Biology, Vol. 48, Issue 6
  • DOI: 10.3109/10409238.2013.840259

The Mediator complex: a master coordinator of transcription and cell lineage development
journal, February 2014


The Mediator complex: a central integrator of transcription
journal, February 2015

  • Allen, Benjamin L.; Taatjes, Dylan J.
  • Nature Reviews Molecular Cell Biology, Vol. 16, Issue 3
  • DOI: 10.1038/nrm3951

FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
journal, May 1999

  • Michaels, Scott D.; Amasino, Richard M.
  • The Plant Cell, Vol. 11, Issue 5
  • DOI: 10.2307/3870827

    Works referencing / citing this record:

    Supplemental Material for Dolan and Chapple, 2018
    dataset, January 2018


    Supplemental Material for Dolan and Chapple, 2018
    dataset, January 2018


    The plant Mediator and its role in noncoding RNA production
    journal, April 2011


    A novel mediator between activator proteins and the RNA polymerase II transcription apparatus
    journal, June 1990


    A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast
    journal, July 1993


    Human Mediator Subunit MED26 Functions as a Docking Site for Transcription Elongation Factors
    journal, July 2011


    Facilitated Assembly of the Preinitiation Complex by Separated Tail and Head/Middle Modules of the Mediator
    journal, January 2012

    • Galdieri, Luciano; Desai, Parima; Vancura, Ales
    • Journal of Molecular Biology, Vol. 415, Issue 3
    • DOI: 10.1016/j.jmb.2011.11.020

    Mediator Expression Profiling Epistasis Reveals a Signal Transduction Pathway with Antagonistic Submodules and Highly Specific Downstream Targets
    journal, August 2005


    Mediator Complex Regulates Alternative mRNA Processing via the MED23 Subunit
    journal, February 2012


    Tail and Kinase Modules Differently Regulate Core Mediator Recruitment and Function In Vivo
    journal, November 2016


    Mediator: A key regulator of plant development
    journal, November 2016


    An Activator Target in the RNA Polymerase II Holoenzyme
    journal, May 1998


    Regulation of flowering time by light quality
    journal, June 2003


    Architecture of the RNA polymerase II–Mediator core initiation complex
    journal, February 2015

    • Plaschka, C.; Larivière, L.; Wenzeck, L.
    • Nature, Vol. 518, Issue 7539
    • DOI: 10.1038/nature14229

    Mediator structure and rearrangements required for holoenzyme formation
    journal, March 2017

    • Tsai, Kuang-Lei; Yu, Xiaodi; Gopalan, Sneha
    • Nature, Vol. 544, Issue 7649
    • DOI: 10.1038/nature21393

    Fiji: an open-source platform for biological-image analysis
    journal, June 2012

    • Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
    • Nature Methods, Vol. 9, Issue 7
    • DOI: 10.1038/nmeth.2019

    Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
    journal, December 2008

    • Huang, Da Wei; Sherman, Brad T.; Lempicki, Richard A.
    • Nature Protocols, Vol. 4, Issue 1
    • DOI: 10.1038/nprot.2008.211

    The Mediator complex: a central integrator of transcription
    journal, February 2015

    • Allen, Benjamin L.; Taatjes, Dylan J.
    • Nature Reviews Molecular Cell Biology, Vol. 16, Issue 3
    • DOI: 10.1038/nrm3951

    CDK8 is a positive regulator of transcriptional elongation within the serum response network
    journal, January 2010

    • Donner, Aaron J.; Ebmeier, Christopher C.; Taatjes, Dylan J.
    • Nature Structural & Molecular Biology, Vol. 17, Issue 2
    • DOI: 10.1038/nsmb.1752

    A conserved Mediator–CDK8 kinase module association regulates Mediator–RNA polymerase II interaction
    journal, April 2013

    • Tsai, Kuang-Lei; Sato, Shigeo; Tomomori-Sato, Chieri
    • Nature Structural & Molecular Biology, Vol. 20, Issue 5
    • DOI: 10.1038/nsmb.2549

    Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit
    journal, November 2014

    • Cevher, Murat A.; Shi, Yi; Li, Dan
    • Nature Structural & Molecular Biology, Vol. 21, Issue 12
    • DOI: 10.1038/nsmb.2914

    The sfr6 mutant of Arabidopsis is defective in transcriptional activation via CBF/DREB1 and DREB2 and shows sensitivity to osmotic stress
    journal, May 2003


    The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
    journal, October 2006

    • Elmlund, H.; Baraznenok, V.; Lindahl, M.
    • Proceedings of the National Academy of Sciences, Vol. 103, Issue 43
    • DOI: 10.1073/pnas.0607483103

    The Arabidopsis thaliana Med25 mediator subunit integrates environmental cues to control plant development
    journal, May 2011

    • Elfving, N.; Davoine, C.; Benlloch, R.
    • Proceedings of the National Academy of Sciences, Vol. 108, Issue 20
    • DOI: 10.1073/pnas.1002981108

    Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator
    journal, February 2014

    • Gonzalez, D.; Hamidi, N.; Del Sol, R.
    • Proceedings of the National Academy of Sciences, Vol. 111, Issue 7
    • DOI: 10.1073/pnas.1307525111

    Inheritance of Trans Chromosomal Methylation patterns from Arabidopsis F1 hybrids
    journal, January 2014

    • Greaves, Ian K.; Groszmann, Michael; Wang, Aihua
    • Proceedings of the National Academy of Sciences, Vol. 111, Issue 5, p. 2017-2022
    • DOI: 10.1073/pnas.1323656111

    Yeast global transcriptional regulators Sin4 and Rgr1 are components of mediator complex/RNA polymerase II holoenzyme.
    journal, November 1995

    • Li, Y.; Bjorklund, S.; Jiang, Y. W.
    • Proceedings of the National Academy of Sciences, Vol. 92, Issue 24
    • DOI: 10.1073/pnas.92.24.10864

    Mediator protein mutations that selectively abolish activated transcription
    journal, January 1999

    • Myers, L. C.; Gustafsson, C. M.; Hayashibara, K. C.
    • Proceedings of the National Academy of Sciences, Vol. 96, Issue 1
    • DOI: 10.1073/pnas.96.1.67

    REF4 and RFR1, Subunits of the Transcriptional Coregulatory Complex Mediator, Are Required for Phenylpropanoid Homeostasis in Arabidopsis
    journal, December 2011

    • Bonawitz, Nicholas D.; Soltau, Whitney L.; Blatchley, Michael R.
    • Journal of Biological Chemistry, Vol. 287, Issue 8
    • DOI: 10.1074/jbc.m111.312298

    The Structural and Functional Role of Med5 in the Yeast Mediator Tail Module
    journal, October 2005

    • Béve, Jenny; Hu, Guo-Zhen; Myers, Lawrence C.
    • Journal of Biological Chemistry, Vol. 280, Issue 50
    • DOI: 10.1074/jbc.m511181200

    TopHat: discovering splice junctions with RNA-Seq
    journal, March 2009


    edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
    journal, November 2009


    HTSeq--a Python framework to work with high-throughput sequencing data
    journal, September 2014


    Small-sample estimation of negative binomial dispersion, with applications to SAGE data
    journal, July 2007


    Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily
    journal, January 2009

    • Dixon, David P.; Hawkins, Timothy; Hussey, Patrick J.
    • Journal of Experimental Botany, Vol. 60, Issue 4
    • DOI: 10.1093/jxb/ern365

    ATTED-II in 2016: A Plant Coexpression Database Towards Lineage-Specific Coexpression
    journal, November 2015

    • Aoki, Yuichi; Okamura, Yasunobu; Tadaka, Shu
    • Plant and Cell Physiology, Vol. 57, Issue 1
    • DOI: 10.1093/pcp/pcv165

    Conservation and Divergence of Mediator Structure and Function: Insights from Plants
    journal, November 2016


    The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function
    journal, February 2009

    • Knuesel, M. T.; Meyer, K. D.; Bernecky, C.
    • Genes & Development, Vol. 23, Issue 4
    • DOI: 10.1101/gad.1767009

    SENSITIVE TO FREEZING6 Integrates Cellular and Environmental Inputs to the Plant Circadian Clock
    journal, July 2008

    • Knight, Heather; Thomson, Adrian J. W.; McWatters, Harriet G.
    • Plant Physiology, Vol. 148, Issue 1
    • DOI: 10.1104/pp.108.123901

    MEDIATOR25 Acts as an Integrative Hub for the Regulation of Jasmonate-Responsive Gene Expression in Arabidopsis
    journal, July 2012

    • Çevik, Volkan; Kidd, Brendan N.; Zhang, Peijun
    • Plant Physiology, Vol. 160, Issue 1
    • DOI: 10.1104/pp.112.202697

    PHYTOCHROME AND FLOWERING TIME1/MEDIATOR25 Regulates Lateral Root Formation via Auxin Signaling in Arabidopsis
    journal, April 2014

    • Raya-González, Javier; Ortiz-Castro, Randy; Ruíz-Herrera, León Francisco
    • Plant Physiology, Vol. 165, Issue 2
    • DOI: 10.1104/pp.114.239806

    The Mediator Complex Subunit PFT1 Is a Key Regulator of Jasmonate-Dependent Defense in Arabidopsis
    journal, August 2009

    • Kidd, Brendan N.; Edgar, Cameron I.; Kumar, Krish K.
    • The Plant Cell, Vol. 21, Issue 8
    • DOI: 10.1105/tpc.109.066910

    Conserved Alternative Splicing of Arabidopsis Transthyretin-Like Determines Protein Localization and S -Allantoin Synthesis in Peroxisomes
    journal, May 2010

    • Lamberto, Ilaria; Percudani, Riccardo; Gatti, Rita
    • The Plant Cell, Vol. 22, Issue 5
    • DOI: 10.1105/tpc.109.070102

    The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif
    journal, May 1999

    • Knight, Heather; Veale, Emma L.; Warren, Gareth J.
    • The Plant Cell, Vol. 11, Issue 5
    • DOI: 10.1105/tpc.11.5.875

    FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
    journal, May 1999

    • Michaels, Scott D.; Amasino, Richard M.
    • The Plant Cell, Vol. 11, Issue 5
    • DOI: 10.1105/tpc.11.5.949

    A Major Facilitator Superfamily Transporter Plays a Dual Role in Polar Auxin Transport and Drought Stress Tolerance in Arabidopsis
    journal, March 2013

    • Remy, Estelle; Cabrito, Tânia R.; Baster, Pawel
    • The Plant Cell, Vol. 25, Issue 3
    • DOI: 10.1105/tpc.113.110353

    Complexity of the Alternative Splicing Landscape in Plants
    journal, October 2013

    • Reddy, Anireddy S. N.; Marquez, Yamile; Kalyna, Maria
    • The Plant Cell, Vol. 25, Issue 10
    • DOI: 10.1105/tpc.113.117523

    The Arabidopsis Mediator Complex Subunits MED16, MED14, and MED2 Regulate Mediator and RNA Polymerase II Recruitment to CBF-Responsive Cold-Regulated Genes
    journal, January 2014

    • Hemsley, Piers A.; Hurst, Charlotte H.; Kaliyadasa, Ewon
    • The Plant Cell, Vol. 26, Issue 1
    • DOI: 10.1105/tpc.113.117796

    Mediator Complex Subunits MED2, MED5, MED16, and MED23 Genetically Interact in the Regulation of Phenylpropanoid Biosynthesis
    journal, December 2017

    • Dolan, Whitney L.; Dilkes, Brian P.; Stout, Jake M.
    • The Plant Cell, Vol. 29, Issue 12
    • DOI: 10.1105/tpc.17.00282

    Identification of SFR6, a key component in cold acclimation acting post-translationally on CBF function: SFR6 and cold acclimation
    journal, January 2009


    Alternative splicing of the auxin biosynthesis gene YUCCA4 determines its subcellular compartmentation: YUCCA4 and auxin biosynthesis
    journal, January 2012


    The Mediator subunit SFR6/MED16 controls defence gene expression mediated by salicylic acid and jasmonate responsive pathways
    journal, April 2012


    Plant Mediator complex and its critical functions in transcription regulation: Plant Mediator complex and its function
    journal, September 2015

    • Yang, Yan; Li, Ling; Qu, Li-Jia
    • Journal of Integrative Plant Biology, Vol. 58, Issue 2
    • DOI: 10.1111/jipb.12377

    PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis
    journal, October 2012

    • Sundaravelpandian, Kalaipandian; Chandrika, Nulu Naga Prafulla; Schmidt, Wolfgang
    • New Phytologist, Vol. 197, Issue 1
    • DOI: 10.1111/nph.12000

    Mediator subunit 16 functions in the regulation of iron uptake gene expression in Arabidopsis
    journal, May 2014

    • Zhang, Yue; Wu, Huilan; Wang, Ning
    • New Phytologist, Vol. 203, Issue 3
    • DOI: 10.1111/nph.12860

    The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
    journal, February 2014

    • Yang, Yan; Ou, Bin; Zhang, Jinzhe
    • The Plant Journal, Vol. 77, Issue 6
    • DOI: 10.1111/tpj.12440

    An Activator Binding Module of Yeast RNA Polymerase II Holoenzyme
    journal, April 1999

    • Lee, Young Chul; Park, Jin Mo; Min, Soyoung
    • Molecular and Cellular Biology, Vol. 19, Issue 4
    • DOI: 10.1128/mcb.19.4.2967

    In Vivo Requirement of Activator-Specific Binding Targets of Mediator
    journal, December 2000


    A Triad of Subunits from the Gal11/Tail Domain of Srb Mediator Is an In Vivo Target of Transcriptional Activator Gcn4p
    journal, July 2004


    ImageJ2: ImageJ for the next generation of scientific image data
    journal, November 2017

    • Rueden, Curtis T.; Schindelin, Johannes; Hiner, Mark C.
    • BMC Bioinformatics, Vol. 18, Issue 1
    • DOI: 10.1186/s12859-017-1934-z

    The MED12-MED13 module of Mediator regulates the timing of embryo patterning in Arabidopsis
    journal, December 2009

    • Gillmor, C. S.; Park, M. Y.; Smith, M. R.
    • Development, Vol. 137, Issue 1
    • DOI: 10.1242/dev.043174

    Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
    journal, September 2011


    The Mediator complex: a master coordinator of transcription and cell lineage development
    journal, February 2014


    Differential Use of Signal Peptides and Membrane Domains Is a Common Occurrence in the Protein Output of Transcriptional Units
    journal, January 2006


    An “Electronic Fluorescent Pictograph” Browser for Exploring and Analyzing Large-Scale Biological Data Sets
    journal, August 2007


    The M ediator subunit MED 23 couples H2B mono‐ubiquitination to transcriptional control and cell fate determination
    journal, September 2015


    The Mediator complex and transcription regulation
    journal, October 2013

    • Poss, Zachary C.; Ebmeier, Christopher C.; Taatjes, Dylan J.
    • Critical Reviews in Biochemistry and Molecular Biology, Vol. 48, Issue 6
    • DOI: 10.3109/10409238.2013.840259

    Importance of Mediator complex in the regulation and integration of diverse signaling pathways in plants
    journal, September 2015


    The Mediator Complex Subunits MED14, MED15, and MED16 Are Involved in Defense Signaling Crosstalk in Arabidopsis
    journal, December 2016


    Mediator MED23 regulates basal transcription in vivo via an interaction with P-TEFb
    journal, January 2013


    Molecular architecture of the yeast Mediator complex
    journal, September 2015

    • Robinson, Philip J.; Trnka, Michael J.; Pellarin, Riccardo
    • eLife, Vol. 4
    • DOI: 10.7554/elife.08719