Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals

Yan, Qiang; Pfleger, Brian F.

doi:10.1016/j.ymben.2019.04.009

Title: Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals

Journal Article · Mon Apr 22 00:00:00 UTC 2019 · Metabolic Engineering

DOI: https://doi.org/10.1016/j.ymben.2019.04.009 · OSTI ID:1509679

Yan, Qiang ^[1]; Pfleger, Brian F. ^[2]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, and DOE Center for Advanced Bioenergy and Bioproducts Innovation; CABBI
Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, DOE Center for Advanced Bioenergy and Bioproducts Innovation, and Microbiology Doctoral Training Program

Microbial production of oleochemicals from renewable feedstocks remains an attractive route to produce high-energy density, liquid transportation fuels and high-value chemical products. Metabolic engineering strategies have been applied to demonstrate production of a wide range of oleochemicals, including free fatty acids, fatty alcohols, esters, olefins, alkanes, ketones, and polyesters in both bacteria and yeast. The majority of these demonstrations synthesized products containing long-chain fatty acids. These successes motivated additional effort to produce analogous molecules comprised of medium-chain fatty acids, molecules that are less common in natural oils and therefore of higher commercial value. Substantial progress has been made towards producing a subset of these chemicals, but significant work remains for most. The other primary challenge to producing oleochemicals in microbes is improving the performance, in terms of yield, rate, and titer, of biocatalysts such that economic large-scale processes are feasible. Common metabolic engineering strategies include blocking pathways that compete with synthesis of oleochemical building blocks and/or consume products, pulling flux through pathways by removing regulatory signals, pushing flux into biosynthesis by overexpressing rate-limiting enzymes, and engineering cells to tolerate the presence of oleochemical products. In this review, we describe the basic fundamentals of oleochemical synthesis and summarize advances since 2013 towards improving performance of heterotrophic microbial cell factories.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018420

OSTI ID:: 1509679

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Vol. 58; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
Beta-oxidation
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Fatty acid
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Saccharomyces cerevisiae
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Title: Revisiting metabolic engineering strategies for microbial synthesis of oleochemicals

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