Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis
Abstract
Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fast. Current genome-to-phenome mapping is still poorly performed for non-model organisms. Even for species with identical genomes, cell phenotypes can be strikingly different. To understand Synechococcus 2973’s fast-growth phenotype and its metabolic features advantageous to photo-biorefineries, 13C isotopically nonstationary metabolic flux analysis (INST-MFA), biomass compositional analysis, gene knockouts, and metabolite profiling were performed on both strains under various growth conditions.
- Publication Date:
- Research Org.:
- Washington Univ., St. Louis, MO (United States). Donald Danforth Plant Science Center
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1618702
- Alternate Identifier(s):
- OSTI ID: 1504898
- Grant/Contract Number:
- DESC0012722; AE-AR0000202; AR0000202; SC0012722
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 13C labeling experiments; Channeling; Glycogen; Metabolites; Photobioreactor; Energy charge
Citation Formats
Abernathy, Mary H., Yu, Jingjie, Ma, Fangfang, Liberton, Michelle, Ungerer, Justin, Hollinshead, Whitney D., Gopalakrishnan, Saratram, He, Lian, Maranas, Costas D., Pakrasi, Himadri B., Allen, Doug K., and Tang, Yinjie J. Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis. Netherlands: N. p., 2017.
Web. doi:10.1186/s13068-017-0958-y.
Abernathy, Mary H., Yu, Jingjie, Ma, Fangfang, Liberton, Michelle, Ungerer, Justin, Hollinshead, Whitney D., Gopalakrishnan, Saratram, He, Lian, Maranas, Costas D., Pakrasi, Himadri B., Allen, Doug K., & Tang, Yinjie J. Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis. Netherlands. https://doi.org/10.1186/s13068-017-0958-y
Abernathy, Mary H., Yu, Jingjie, Ma, Fangfang, Liberton, Michelle, Ungerer, Justin, Hollinshead, Whitney D., Gopalakrishnan, Saratram, He, Lian, Maranas, Costas D., Pakrasi, Himadri B., Allen, Doug K., and Tang, Yinjie J. Thu .
"Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis". Netherlands. https://doi.org/10.1186/s13068-017-0958-y.
@article{osti_1618702,
title = {Deciphering cyanobacterial phenotypes for fast photoautotrophic growth via isotopically nonstationary metabolic flux analysis},
author = {Abernathy, Mary H. and Yu, Jingjie and Ma, Fangfang and Liberton, Michelle and Ungerer, Justin and Hollinshead, Whitney D. and Gopalakrishnan, Saratram and He, Lian and Maranas, Costas D. and Pakrasi, Himadri B. and Allen, Doug K. and Tang, Yinjie J.},
abstractNote = {Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fast. Current genome-to-phenome mapping is still poorly performed for non-model organisms. Even for species with identical genomes, cell phenotypes can be strikingly different. To understand Synechococcus 2973’s fast-growth phenotype and its metabolic features advantageous to photo-biorefineries, 13C isotopically nonstationary metabolic flux analysis (INST-MFA), biomass compositional analysis, gene knockouts, and metabolite profiling were performed on both strains under various growth conditions.},
doi = {10.1186/s13068-017-0958-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Thu Nov 16 00:00:00 EST 2017},
month = {Thu Nov 16 00:00:00 EST 2017}
}
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https://doi.org/10.1186/s13068-017-0958-y
https://doi.org/10.1186/s13068-017-0958-y
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