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Title: Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production

Abstract

BackgroundLignocellulosic biomass is viewed as an abundant renewable source of liquid fuels and chemicals that are currently derived from petroleum. When lignocellulosic biomass is used for ethanol production, the resulting liquid residue (stillage) contains large amounts of organic material that could be further transformed into recoverable bioproducts, hence enhancing the economics of the biorefinery.ResultsHere we test the hypothesis that a bacterial community could transform the organics in stillage into valuable bioproducts. We demonstrate the ability of this microbiome to convert stillage organics into medium-chain fatty acids (MCFAs), identify the predominant community members, and perform a technoeconomic analysis of recovering MCFAs as co-products of ethanol production. Steady-state operation of a stillage-fed bioreactor showed that 18% of the organic matter in stillage was converted to MCFAs. Xylose and complex carbohydrates were the primary substrates transformed. During the MCFA production period, the five major genera represented more than 95% of the community, including Lactobacillus, Roseburia, Atopobium, Olsenella, and Pseudoramibacter. To assess the potential benefits of producing MCFAs from stillage, we modeled the economics of ethanol and MCFA co-production, at MCFA productivities observed during reactor operation. ConclusionsThe analysis predicts that production of MCFAs, ethanol, and electricity could reduce the minimum ethanol selling price frommore » 2.15 dollars 1.76 dollars gal-1 ($2.68 gal-1 gasoline equivalents) when compared to a lignocellulosic biorefinery that produces only ethanol and electricity.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1504752
Alternate Identifier(s):
OSTI ID: 1506424
Grant/Contract Number:  
FC02-07ER64494; SC0018409
Resource Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Medium-chain fatty acids; Mixed culture fermentation; Carboxylate platform; Chain elongation; Lignocellulose; Stillage

Citation Formats

Scarborough, Matthew J., Lynch, Griffin, Dickson, Mitch, McGee, Mick, Donohue, Timothy J., and Noguera, Daniel R. Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production. United States: N. p., 2018. Web. doi:10.1186/s13068-018-1193-x.
Scarborough, Matthew J., Lynch, Griffin, Dickson, Mitch, McGee, Mick, Donohue, Timothy J., & Noguera, Daniel R. Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production. United States. doi:10.1186/s13068-018-1193-x.
Scarborough, Matthew J., Lynch, Griffin, Dickson, Mitch, McGee, Mick, Donohue, Timothy J., and Noguera, Daniel R. Thu . "Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production". United States. doi:10.1186/s13068-018-1193-x. https://www.osti.gov/servlets/purl/1504752.
@article{osti_1504752,
title = {Increasing the economic value of lignocellulosic stillage through medium-chain fatty acid production},
author = {Scarborough, Matthew J. and Lynch, Griffin and Dickson, Mitch and McGee, Mick and Donohue, Timothy J. and Noguera, Daniel R.},
abstractNote = {BackgroundLignocellulosic biomass is viewed as an abundant renewable source of liquid fuels and chemicals that are currently derived from petroleum. When lignocellulosic biomass is used for ethanol production, the resulting liquid residue (stillage) contains large amounts of organic material that could be further transformed into recoverable bioproducts, hence enhancing the economics of the biorefinery.ResultsHere we test the hypothesis that a bacterial community could transform the organics in stillage into valuable bioproducts. We demonstrate the ability of this microbiome to convert stillage organics into medium-chain fatty acids (MCFAs), identify the predominant community members, and perform a technoeconomic analysis of recovering MCFAs as co-products of ethanol production. Steady-state operation of a stillage-fed bioreactor showed that 18% of the organic matter in stillage was converted to MCFAs. Xylose and complex carbohydrates were the primary substrates transformed. During the MCFA production period, the five major genera represented more than 95% of the community, including Lactobacillus, Roseburia, Atopobium, Olsenella, and Pseudoramibacter. To assess the potential benefits of producing MCFAs from stillage, we modeled the economics of ethanol and MCFA co-production, at MCFA productivities observed during reactor operation. ConclusionsThe analysis predicts that production of MCFAs, ethanol, and electricity could reduce the minimum ethanol selling price from 2.15 dollars 1.76 dollars gal-1 ($2.68 gal-1 gasoline equivalents) when compared to a lignocellulosic biorefinery that produces only ethanol and electricity.},
doi = {10.1186/s13068-018-1193-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {7}
}

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Cited by: 12 works
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Works referenced in this record:

Metabolic and microbial community dynamics during the anaerobic digestion of maize silage in a two-phase process
journal, September 2015

  • Sträuber, Heike; Lucas, Rico; Kleinsteuber, Sabine
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 1
  • DOI: 10.1007/s00253-015-6996-0

Xylose utilization in ethanol production: a patent landscape: Xylose Utilization in Ethanol Production: A Patent Landscape
journal, July 2016

  • Devarapalli, Pratap; Deshpande, Nishad; Hirwani, Rajkumar R.
  • Biofuels, Bioproducts and Biorefining, Vol. 10, Issue 5
  • DOI: 10.1002/bbb.1664

Microbiome-based carboxylic acids production: from serum bottles to bioreactors
journal, January 2017

  • Hegner, Richard; Koch, Christin; Riechert, Vanessa
  • RSC Advances, Vol. 7, Issue 25
  • DOI: 10.1039/C6RA28259H

Microbial synthesis of medium-chain chemicals from renewables
journal, December 2017

  • Sarria, Stephen; Kruyer, Nicholas S.; Peralta-Yahya, Pamela
  • Nature Biotechnology, Vol. 35, Issue 12
  • DOI: 10.1038/nbt.4022

PyNAST: a flexible tool for aligning sequences to a template alignment
journal, November 2009


Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces
journal, October 2006

  • Duncan, S. H.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 56, Issue 10
  • DOI: 10.1099/ijs.0.64098-0

Electrolytic extraction drives volatile fatty acid chain elongation through lactic acid and replaces chemical pH control in thin stillage fermentation
journal, December 2015

  • Andersen, Stephen J.; Candry, Pieter; Basadre, Thais
  • Biotechnology for Biofuels, Vol. 8, Issue 1
  • DOI: 10.1186/s13068-015-0396-7

Draft Genome Sequence of the Mannitol-Producing Strain Lactobacillus mucosae CRL573
journal, November 2014


Life cycle assessment of lignocellulosic ethanol: a review of key factors and methods affecting calculated GHG emissions and energy use
journal, April 2016


Amended description of Ramibacterium alactolyticum Prevot and Taffanel with proposal of a neotype strain
journal, October 1967

  • Holdeman, L. V.; Cato, E. P.; Moore, W. E. C.
  • International Journal of Systematic Bacteriology, Vol. 17, Issue 4
  • DOI: 10.1099/00207713-17-4-323

Upgrading dilute ethanol from syngas fermentation to n-caproate with reactor microbiomes
journal, January 2014


Complete genome sequence of Lactobacillus plantarum WCFS1
journal, February 2003

  • Kleerebezem, M.; Boekhorst, J.; van Kranenburg, R.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 4
  • DOI: 10.1073/pnas.0337704100

Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris
journal, July 2015

  • Austin, Samantha; Kontur, Wayne S.; Ulbrich, Arne
  • Environmental Science & Technology, Vol. 49, Issue 14
  • DOI: 10.1021/acs.est.5b02062

Lactic acid production from biomass-derived sugars via co-fermentation of Lactobacillus brevis and Lactobacillus plantarum
journal, June 2015


Conversion of l-lactate into n-caproate by a continuously fed reactor microbiome
journal, April 2016


Glycerol production by microbial fermentation
journal, June 2001


Characterization of a novel Atopobium isolate from the human vagina: description of Atopobium vaginae sp. nov.
journal, October 1999

  • Jovita, M. R.; Collins, M. D.; Sjoden, B.
  • International Journal of Systematic Bacteriology, Vol. 49, Issue 4
  • DOI: 10.1099/00207713-49-4-1573

Measurement of protein using bicinchoninic acid
journal, October 1985


FLASH: fast length adjustment of short reads to improve genome assemblies
journal, September 2011


Large-scale production, harvest and logistics of switchgrass ( Panicum virgatum L. ) - current technology and envisioning a mature technology
journal, March 2009

  • Sokhansanj, Shahab; Mani, Sudhagar; Turhollow, Anthony
  • Biofuels, Bioproducts and Biorefining, Vol. 3, Issue 2
  • DOI: 10.1002/bbb.129

Lactobacillus odoratitofui sp. nov., isolated from stinky tofu brine
journal, January 2010

  • Chao, S. -H.; Sasamoto, M.; Kudo, Y.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 60, Issue 12
  • DOI: 10.1099/ijs.0.019307-0

Chain Elongation with Reactor Microbiomes: Open-Culture Biotechnology To Produce Biochemicals
journal, February 2016

  • Angenent, Largus T.; Richter, Hanno; Buckel, Wolfgang
  • Environmental Science & Technology, Vol. 50, Issue 6
  • DOI: 10.1021/acs.est.5b04847

The estimation of carbohydrates in plant extracts by anthrone
journal, July 1954

  • Yemm, E. W.; Willis, A. J.
  • Biochemical Journal, Vol. 57, Issue 3
  • DOI: 10.1042/bj0570508

Biochemical Analysis of a  -D-Xylosidase and a Bifunctional Xylanase-Ferulic Acid Esterase from a Xylanolytic Gene Cluster in Prevotella ruminicola 23
journal, March 2009

  • Dodd, D.; Kocherginskaya, S. A.; Spies, M. A.
  • Journal of Bacteriology, Vol. 191, Issue 10
  • DOI: 10.1128/JB.01628-08

Search and clustering orders of magnitude faster than BLAST
journal, August 2010


phyloseq: An R Package for Reproducible Interactive Analysis and Graphics of Microbiome Census Data
journal, April 2013


Inhibition of microbial biofuel production in drought-stressed switchgrass hydrolysate
journal, November 2016

  • Ong, Rebecca Garlock; Higbee, Alan; Bottoms, Scott
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0657-0

Mixed Carboxylic Acid Production by Megasphaera elsdenii from Glucose and Lignocellulosic Hydrolysate
journal, March 2017


The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production
journal, September 2015

  • Zhu, Xiaoyu; Tao, Yong; Liang, Cheng
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep14360

RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies
journal, January 2014


Production of medium-chain carboxylic acids by Megasphaera sp. MH with supplemental electron acceptors
journal, June 2016

  • Jeon, Byoung Seung; Choi, Okkyoung; Um, Youngsoon
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0549-3

Lactobacillus diolivorans sp. nov., a 1,2-propanediol-degrading bacterium isolated from aerobically stable maize silage.
journal, March 2002

  • Gottschal, J. C.; Alderkamp, A. C.; Elferink, S. J. H. W. Oude
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 52, Issue 2
  • DOI: 10.1099/00207713-52-2-639

A Clostridium Group IV Species Dominates and Suppresses a Mixed Culture Fermentation by Tolerance to Medium Chain Fatty Acids Products
journal, February 2017

  • Andersen, Stephen J.; De Groof, Vicky; Khor, Way Cern
  • Frontiers in Bioengineering and Biotechnology, Vol. 5
  • DOI: 10.3389/fbioe.2017.00008

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies
journal, August 2012

  • Klindworth, Anna; Pruesse, Elmar; Schweer, Timmy
  • Nucleic Acids Research, Vol. 41, Issue 1
  • DOI: 10.1093/nar/gks808

The SILVA ribosomal RNA gene database project: improved data processing and web-based tools
journal, November 2012

  • Quast, Christian; Pruesse, Elmar; Yilmaz, Pelin
  • Nucleic Acids Research, Vol. 41, Issue D1
  • DOI: 10.1093/nar/gks1219

Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine
journal, January 2000

  • Roos, S.; Karner, F.; Axelsson, L.
  • INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 50, Issue 1
  • DOI: 10.1099/00207713-50-1-251

Anaerobic digestion for treatment of stillage from cellulosic bioethanol production
journal, September 2013


Roseburia cecicola gen. nov., sp. nov., a Motile, Obligately Anaerobic Bacterium from a Mouse Cecum
journal, July 1983

  • Stanton, T. B.; Savage, D. C.
  • International Journal of Systematic Bacteriology, Vol. 33, Issue 3
  • DOI: 10.1099/00207713-33-3-618

Comparative assessment of single-stage and two-stage anaerobic digestion for the treatment of thin stillage
journal, May 2012


Long-Term n -Caproic Acid Production from Yeast-Fermentation Beer in an Anaerobic Bioreactor with Continuous Product Extraction
journal, June 2015

  • Ge, Shijian; Usack, Joseph G.; Spirito, Catherine M.
  • Environmental Science & Technology, Vol. 49, Issue 13
  • DOI: 10.1021/acs.est.5b00238

QIIME allows analysis of high-throughput community sequencing data
journal, April 2010

  • Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
  • Nature Methods, Vol. 7, Issue 5
  • DOI: 10.1038/nmeth.f.303

High n-caprylate productivities and specificities from dilute ethanol and acetate: chain elongation with microbiomes to upgrade products from syngas fermentation
journal, January 2016

  • Kucek, Leo A.; Spirito, Catherine M.; Angenent, Largus T.
  • Energy & Environmental Science, Vol. 9, Issue 11
  • DOI: 10.1039/C6EE01487A

Lactic acid production from wheat straw hemicellulose hydrolysate by Lactobacillus pentosus and Lactobacillus brevis
journal, February 2002


Chain elongation with reactor microbiomes: upgrading dilute ethanol to medium-chain carboxylates
journal, January 2012

  • Agler, Matthew T.; Spirito, Catherine M.; Usack, Joseph G.
  • Energy & Environmental Science, Vol. 5, Issue 8
  • DOI: 10.1039/c2ee22101b

Production of drop-in fuels from biomass at high selectivity by combined microbial and electrochemical conversion
journal, January 2017

  • Urban, Carolin; Xu, Jiajie; Sträuber, Heike
  • Energy & Environmental Science, Vol. 10, Issue 10
  • DOI: 10.1039/C7EE01303E

Sustainable bioenergy production from marginal lands in the US Midwest
journal, January 2013

  • Gelfand, Ilya; Sahajpal, Ritvik; Zhang, Xuesong
  • Nature, Vol. 493, Issue 7433
  • DOI: 10.1038/nature11811

Carboxylate Platform: The MixAlco Process Part 1: Comparison of Three Biomass Conversion Platforms
journal, January 2009

  • Holtzapple, Mark T.; Granda, Cesar B.
  • Applied Biochemistry and Biotechnology, Vol. 156, Issue 1-3
  • DOI: 10.1007/s12010-008-8466-y

MUSCLE: multiple sequence alignment with high accuracy and high throughput
journal, March 2004

  • Edgar, R. C.
  • Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797
  • DOI: 10.1093/nar/gkh340

Olsenella scatoligenes sp. nov., a 3-methylindole- (skatole) and 4-methylphenol- (p-cresol) producing bacterium isolated from pig faeces
journal, April 2015

  • Li, Xiaoqiong; Canibe, Nuria; Jensen, Bent Borg
  • International Journal of Systematic and Evolutionary Microbiology, Vol. 65, Issue 4
  • DOI: 10.1099/ijs.0.000083

Waste to bioproduct conversion with undefined mixed cultures: the carboxylate platform
journal, February 2011


Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
journal, January 2011


Superheat: An R Package for Creating Beautiful and Extendable Heatmaps for Visualizing Complex Data
journal, July 2018


    Works referencing / citing this record:

    Key sub-community dynamics of medium-chain carboxylate production
    journal, May 2019

    • Lambrecht, Johannes; Cichocki, Nicolas; Schattenberg, Florian
    • Microbial Cell Factories, Vol. 18, Issue 1
    • DOI: 10.1186/s12934-019-1143-8

    Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation
    journal, January 2019


    Key sub-community dynamics of medium-chain carboxylate production
    journal, May 2019

    • Lambrecht, Johannes; Cichocki, Nicolas; Schattenberg, Florian
    • Microbial Cell Factories, Vol. 18, Issue 1
    • DOI: 10.1186/s12934-019-1143-8

    Medium Chain Carboxylic Acids from Complex Organic Feedstocks by Mixed Culture Fermentation
    journal, January 2019