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Title: Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility

Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Furthermore utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ; ORCiD logo [1]
  1. Syracuse Univ., Syracuse, NY (United States)
  2. Syracuse Univ., Syracuse, NY (United States); Scion, Rotorua (New Zealand)
Publication Date:
Grant/Contract Number:
SC0010411
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Syracuse Univ., Syracuse, NY (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Hyperthermophilic cellulase; Transgenic poplar; Cell wall composition; Saccharification; Biofuel production
OSTI Identifier:
1504257

Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, and Coleman, Heather D. Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility. United States: N. p., Web. doi:10.1186/s13068-018-1224-7.
Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, & Coleman, Heather D. Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility. United States. doi:10.1186/s13068-018-1224-7.
Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, and Coleman, Heather D. 2018. "Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility". United States. doi:10.1186/s13068-018-1224-7. https://www.osti.gov/servlets/purl/1504257.
@article{osti_1504257,
title = {Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility},
author = {Xiao, Yao and He, Xuejun and Ojeda-Lassalle, Yemaiza and Poovaiah, Charleson and Coleman, Heather D.},
abstractNote = {Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Furthermore utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates.},
doi = {10.1186/s13068-018-1224-7},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 11,
place = {United States},
year = {2018},
month = {8}
}

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Cell-wall carbohydrates and their modification as a resource for biofuels
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Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
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