Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility

Xiao, Yao; He, Xuejun; Ojeda-Lassalle, Yemaiza; Poovaiah, Charleson; Coleman, Heather D.

doi:10.1186/s13068-018-1224-7

Title: Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility

Abstract

Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Furthermore utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates.

Authors:

Xiao, Yao; He, Xuejun; Ojeda-Lassalle, Yemaiza; Poovaiah, Charleson;

Publication Date:: Thu Aug 16 00:00:00 EDT 2018

Research Org.:: Syracuse Univ., NY (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1618743

Alternate Identifier(s):: OSTI ID: 1504257

Grant/Contract Number:: SC0010411

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 11 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Hyperthermophilic cellulase; Transgenic poplar; Cell wall composition; Saccharification; Biofuel production

Citation Formats


                    Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, and Coleman, Heather D. Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.  Netherlands: N. p., 2018. 
Web.  doi:10.1186/s13068-018-1224-7.

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                    Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, & Coleman, Heather D. Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility.  Netherlands.  https://doi.org/10.1186/s13068-018-1224-7

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                    Xiao, Yao, He, Xuejun, Ojeda-Lassalle, Yemaiza, Poovaiah, Charleson, and Coleman, Heather D. Thu .  
"Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility".  Netherlands.  https://doi.org/10.1186/s13068-018-1224-7.

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@article{osti_1618743,

  title        = {Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility},

  author       = {Xiao, Yao and He, Xuejun and Ojeda-Lassalle, Yemaiza and Poovaiah, Charleson and Coleman, Heather D.},

  abstractNote = {Expression of glycosyl hydrolases in lignocellulosic biomass has been proposed as an alternative to improve efficiency of cellulosic ethanol production. In planta production of hyperthermophilic hydrolytic enzymes could prevent the detrimental effects often seen resulting from the expression of recombinant mesophilic enzymes to plant hosts. Furthermore utilizing lignocellulosic feedstocks to produce hyperthermophilic hydrolases provides additional benefits for ethanol production in the way of transgenic feedstocks serving as both enzyme providers and cellulosic substrates.},

  doi          = {10.1186/s13068-018-1224-7},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 11,

  place        = {Netherlands},

  year         = {Thu Aug 16 00:00:00 EDT 2018},

  month        = {Thu Aug 16 00:00:00 EDT 2018}

}

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Works referenced in this record:

Global and grain-specific accumulation of glycoside hydrolase family 10 xylanases in transgenic maize (Zea mays): Maize xylanase expression
journal, June 2011

Gray, Benjamin N.; Bougri, Oleg; Carlson, Alvar R.
Plant Biotechnology Journal, Vol. 9, Issue 9
DOI: 10.1111/j.1467-7652.2011.00632.x

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
journal, January 2015

Klose, Holger; Günl, Markus; Usadel, Björn
BMC Plant Biology, Vol. 15, Issue 1
DOI: 10.1186/s12870-015-0443-3

Cell-wall carbohydrates and their modification as a resource for biofuels
journal, May 2008

Pauly, Markus; Keegstra, Kenneth
The Plant Journal, Vol. 54, Issue 4, p. 559-568
DOI: 10.1111/j.1365-313X.2008.03463.x

Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
journal, November 2007

Sun, Ye; Cheng, Jay J.; Himmel, Michael E.
Bioresource Technology, Vol. 98, Issue 15, p. 2866-2872
DOI: 10.1016/j.biortech.2006.09.055

Growth enhancement of transgenic poplar plants by overexpression of Arabidopsis thaliana endo-1,4- -glucanase (cel1)
journal, January 2004

Shani, Ziv; Dekel, Mara; Tsabary, Galit
Molecular Breeding, Vol. 14, Issue 3
DOI: 10.1023/B:MOLB.0000049213.15952.8a

Biosynthesis of plant cell wall polysaccharides — a complex process
journal, December 2006

Lerouxel, Olivier; Cavalier, David M.; Liepman, Aaron H.
Current Opinion in Plant Biology, Vol. 9, Issue 6
DOI: 10.1016/j.pbi.2006.09.009

Analyzing real-time PCR data by the comparative CT method
journal, June 2008

Schmittgen, Thomas D.; Livak, Kenneth J.
Nature Protocols, Vol. 3, Issue 6
DOI: 10.1038/nprot.2008.73

In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion
journal, September 2017

Mir, Bilal Ahmad; Myburg, Alexander A.; Mizrachi, Eshchar
Scientific Reports, Vol. 7, Issue 1
DOI: 10.1038/s41598-017-11026-1

Expression of a fungal glucuronoyl esterase in Populus: Effects on wood properties and saccharification efficiency
journal, April 2015

Latha Gandla, Madhavi; Derba-Maceluch, Marta; Liu, Xiaokun
Phytochemistry, Vol. 112
DOI: 10.1016/j.phytochem.2014.06.002

Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction
journal, February 2005

Cao, Yu; Tan, Huimin
Enzyme and Microbial Technology, Vol. 36, Issue 2-3
DOI: 10.1016/j.enzmictec.2004.09.002

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production
journal, June 2016

Xiao, Yao; Poovaiah, Charleson; Coleman, Heather D.
BioEnergy Research, Vol. 9, Issue 4
DOI: 10.1007/s12155-016-9766-7

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development
journal, January 2013

Klose, Holger; Günl, Markus; Usadel, Björn
Biotechnology for Biofuels, Vol. 6, Issue 1
DOI: 10.1186/1754-6834-6-53

Extremely thermophilic microorganisms for biomass conversion status and prospects
journal, June 2008

Blumer-Schuette, Sara E.; Kataeva, Irina; Westpheling, Janet
Current Opinion in Biotechnology, Vol. 19, Issue 3, p. 210-217
DOI: 10.1016/j.copbio.2008.04.007

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels
journal, August 2008

Taylor, Larry E.; Dai, Ziyu; Decker, Stephen R.
Trends in Biotechnology, Vol. 26, Issue 8
DOI: 10.1016/j.tibtech.2008.05.002

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems
journal, April 2009

Takahashi, Junko; Rudsander, Ulla J.; Hedenström, Mattias
Plant and Cell Physiology, Vol. 50, Issue 6
DOI: 10.1093/pcp/pcp062

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production
journal, December 2016

Bewg, William P.; Poovaiah, Charleson; Lan, Wu
Biotechnology for Biofuels, Vol. 9, Issue 1
DOI: 10.1186/s13068-016-0683-y

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility
journal, January 2014

Badhan, Ajay; Jin, Long; Wang, Yuxi
Biotechnology for Biofuels, Vol. 7, Issue 1
DOI: 10.1186/1754-6834-7-39

OPTIMIZER: a web server for optimizing the codon usage of DNA sequences
journal, May 2007

Puigbo, P.; Guzman, E.; Romeu, A.
Nucleic Acids Research, Vol. 35, Issue Web Server
DOI: 10.1093/nar/gkm219

In Plant Activation: An Inducible, Hyperexpression Platform for Recombinant Protein Production in Plants
journal, July 2013

Dugdale, B.; Mortimer, C. L.; Kato, M.
The Plant Cell, Vol. 25, Issue 7
DOI: 10.1105/tpc.113.113944

Purification, Characterization, and Molecular Analysis of Thermostable Cellulases CelA and CelB fromThermotoga neapolitana
journal, December 1998

Bok, Jin-Duck; Yernool, Dinesh A.; Eveleigh, Douglas E.
Applied and Environmental Microbiology, Vol. 64, Issue 12
DOI: 10.1128/AEM.64.12.4774-4781.1998

Poplar as a feedstock for biofuels: A review of compositional characteristics
journal, March 2010

Sannigrahi, Poulomi; Ragauskas, Arthur J.; Tuskan, Gerald A.
Biofuels, Bioproducts and Biorefining, Vol. 4, Issue 2
DOI: 10.1002/bbb.206

Recombinant hyperthermophilic enzyme expression in plants: a novel approach for lignocellulose digestion
journal, May 2014

Mir, Bilal Ahmad; Mewalal, Ritesh; Mizrachi, Eshchar
Trends in Biotechnology, Vol. 32, Issue 5
DOI: 10.1016/j.tibtech.2014.03.003

An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants
journal, December 2002

Côté, C.; Rutledge, R. G.
Plant Cell Reports, Vol. 21, Issue 6
DOI: 10.1007/s00299-002-0543-z

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group
journal, January 1987

Cornelissen, Ben J. C.; Horowitz, Jeannine; van Kan, Jan A. L.
Nucleic Acids Research, Vol. 15, Issue 17
DOI: 10.1093/nar/15.17.6799

Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases
journal, April 2010

Borkhardt, Bernhard; Harholt, Jesper; Ulvskov, Peter
Plant Biotechnology Journal, Vol. 8, Issue 3
DOI: 10.1111/j.1467-7652.2010.00506.x

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
journal, March 1959

Miller, G. L.
Analytical Chemistry, Vol. 31, Issue 3
DOI: 10.1021/ac60147a030

Significant Increases in Pulping Efficiency in C4H-F5H-Transformed Poplars: Improved Chemical Savings and Reduced Environmental Toxins
journal, October 2003

Huntley, Shannon K.; Ellis, Dave; Gilbert, Margarita
Journal of Agricultural and Food Chemistry, Vol. 51, Issue 21
DOI: 10.1021/jf034320o

Feasibility of Large-Scale Biofuel Production
journal, October 1997

Giampietro, Mario; Ulgiati, Sergio; Pimentel, David
BioScience, Vol. 47, Issue 9
DOI: 10.2307/1313165

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

Brunecky, Roman; Selig, Michael J.; Vinzant, Todd B.
Biotechnology for Biofuels, Vol. 4, Issue 1
DOI: 10.1186/1754-6834-4-1

Extreme Thermophilic Enzyme CelB-m Efficiently Degrades the Cellulose in Transgenic Arabidopsis thaliana
journal, July 2015

Wu, Jiandong; Wang, Meng; Zhang, Hui
Applied Biochemistry and Biotechnology, Vol. 177, Issue 2
DOI: 10.1007/s12010-015-1748-2

Hyperthermophilic endoglucanase for in planta lignocellulose conversion
journal, January 2012

Klose, Holger; Röder, Juliane; Girfoglio, Michele
Biotechnology for Biofuels, Vol. 5, Issue 1
DOI: 10.1186/1754-6834-5-63

Inhibition of enzymatic hydrolysis by residual lignins from softwood-study of enzyme binding and inactivation on lignin-rich surface
journal, June 2011

Rahikainen, Jenni; Mikander, Saara; Marjamaa, Kaisa
Biotechnology and Bioengineering, Vol. 108, Issue 12
DOI: 10.1002/bit.23242

Characterization and varied expression of a membrane-bound endo-β-1,4-glucanase in hybrid poplar
journal, April 2010

Maloney, Victoria J.; Mansfield, Shawn D.
Plant Biotechnology Journal, Vol. 8, Issue 3
DOI: 10.1111/j.1467-7652.2009.00483.x

Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes
journal, July 2011

Zhang, Dongcheng; VanFossen, Amy L.; Pagano, Ryan M.
BioEnergy Research, Vol. 4, Issue 4
DOI: 10.1007/s12155-011-9138-2

Efficient Agrobacterium-mediated transformation of commercial hybrid poplar Populus nigra L. × P. maximowiczii A. Henry
journal, January 2010

Yevtushenko, Dmytro P.; Misra, Santosh
Plant Cell Reports, Vol. 29, Issue 3
DOI: 10.1007/s00299-009-0806-z

Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
journal, May 2010

Kimura, Tetsuya; Mizutani, Tomomi; Sun, Jia-Lin
Bioscience, Biotechnology, and Biochemistry, Vol. 74, Issue 5
DOI: 10.1271/bbb.90774

Evolution of development of vascular cambia and secondary growth: Tansley review
journal, April 2010

Spicer, Rachel; Groover, Andrew
New Phytologist, Vol. 186, Issue 3
DOI: 10.1111/j.1469-8137.2010.03236.x

Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates
journal, April 1991

Gusakov, Alexander V.; Protas, Oksana V.; Chernoglazov, Vladimir M.
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1073, Issue 3
DOI: 10.1016/0304-4165(91)90219-7

Generation of transgenic wheat ( Triticum aestivum L.) accumulating heterologous endo-xylanase or ferulic acid esterase in the endosperm
journal, April 2010

Harholt, Jesper; Bach, Inga C.; Lind-Bouquin, Solveig
Plant Biotechnology Journal, Vol. 8, Issue 3
DOI: 10.1111/j.1467-7652.2009.00490.x

An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
journal, October 1959

Segal, L.; Creely, J. J.; Martin, A. E.
Textile Research Journal, Vol. 29, Issue 10
DOI: 10.1177/004051755902901003

Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010

Park, Sunkyu; Baker, John O.; Himmel, Michael E.
Biotechnology for Biofuels, Vol. 3, Issue 1
DOI: 10.1186/1754-6834-3-10

Effect of bio-treatment on the lipophilic and hydrophilic extractives of wheat straw
journal, June 2009

Qin, M. H.; Xu, Q. H.; Shao, Z. Y.
Bioresource Technology, Vol. 100, Issue 12
DOI: 10.1016/j.biortech.2009.01.055

The Plastochron Index as Applied to Developmental Studies of Cottonwood
journal, March 1971

Larson, Philip R.; Isebrands, J. G.
Canadian Journal of Forest Research, Vol. 1, Issue 1
DOI: 10.1139/x71-001

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
journal, June 2017

Donohoe, Bryon S.; Wei, Hui; Mittal, Ashutosh
Scientific Reports, Vol. 7, Issue 1
DOI: 10.1038/s41598-017-04502-1

Grain and cellulosic ethanol: History, economics, and energy policy
journal, June 2007

Solomon, Barry D.; Barnes, Justin R.; Halvorsen, Kathleen E.
Biomass and Bioenergy, Vol. 31, Issue 6
DOI: 10.1016/j.biombioe.2007.01.023

Development of an Efficient Protocol of RNA Isolation from Recalcitrant Tree Tissues
journal, August 2007

Wang, Xuchu; Tian, Weimin; Li, Yinxin
Molecular Biotechnology, Vol. 38, Issue 1
DOI: 10.1007/s12033-007-0073-6

Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants
journal, November 2006

Biswas, Gadab C. Ghosh; Ransom, Callista; Sticklen, Mariam
Plant Science, Vol. 171, Issue 5, p. 617-623
DOI: 10.1016/j.plantsci.2006.06.004

High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome
journal, February 2011

Petersen, Kerstin; Bock, Ralph
Plant Molecular Biology, Vol. 76, Issue 3-5
DOI: 10.1007/s11103-011-9742-8

The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release
journal, November 2017

Willis, Jonathan D.; Grant, Joshua N.; Mazarei, Mitra
Biotechnology for Biofuels, Vol. 10, Issue 1
DOI: 10.1186/s13068-017-0918-6

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan
journal, August 1988

Chernoglazov, Vladimir M.; Ermolova, Olga V.; Klyosov, Anatole A.
Enzyme and Microbial Technology, Vol. 10, Issue 8, p. 503-507
DOI: 10.1016/0141-0229(88)90029-4

Pretreatment: the key to unlocking low-cost cellulosic ethanol
journal, January 2008

Yang, Bin; Wyman, Charles E.
Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1
DOI: 10.1002/bbb.49

Expression of a Trichoderma reesei β-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses
journal, August 2012

Buanafina, Marcia M. de O.; Langdon, Tim; Dalton, Sue
Planta, Vol. 236, Issue 6
DOI: 10.1007/s00425-012-1724-9

Wood modification by heat treatment: A review
journal, December 2008

Esteves, Bruno M.; Pereira, Helena M.
BioResources, Vol. 4, Issue 1
DOI: 10.15376/biores.4.1.370-404

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Title: Expression of a hyperthermophilic endoglucanase in hybrid poplar modifies the plant cell wall and enhances digestibility

Abstract

Citation Formats

Global and grain-specific accumulation of glycoside hydrolase family 10 xylanases in transgenic maize (Zea mays): Maize xylanase expression journal, June 2011

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei journal, January 2015

Cell-wall carbohydrates and their modification as a resource for biofuels journal, May 2008

Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627 journal, November 2007

Growth enhancement of transgenic poplar plants by overexpression of Arabidopsis thaliana endo-1,4- -glucanase (cel1) journal, January 2004

Biosynthesis of plant cell wall polysaccharides — a complex process journal, December 2006

Analyzing real-time PCR data by the comparative CT method journal, June 2008

In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion journal, September 2017

Expression of a fungal glucuronoyl esterase in Populus: Effects on wood properties and saccharification efficiency journal, April 2015

Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction journal, February 2005

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production journal, June 2016

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development journal, January 2013

Extremely thermophilic microorganisms for biomass conversion status and prospects journal, June 2008

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels journal, August 2008

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems journal, April 2009

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In Plant Activation: An Inducible, Hyperexpression Platform for Recombinant Protein Production in Plants journal, July 2013

Purification, Characterization, and Molecular Analysis of Thermostable Cellulases CelA and CelB fromThermotoga neapolitana journal, December 1998

Poplar as a feedstock for biofuels: A review of compositional characteristics journal, March 2010

Recombinant hyperthermophilic enzyme expression in plants: a novel approach for lignocellulose digestion journal, May 2014

An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants journal, December 2002

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group journal, January 1987

Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases journal, April 2010

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar journal, March 1959

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Feasibility of Large-Scale Biofuel Production journal, October 1997

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize journal, January 2011

Extreme Thermophilic Enzyme CelB-m Efficiently Degrades the Cellulose in Transgenic Arabidopsis thaliana journal, July 2015

Hyperthermophilic endoglucanase for in planta lignocellulose conversion journal, January 2012

Inhibition of enzymatic hydrolysis by residual lignins from softwood-study of enzyme binding and inactivation on lignin-rich surface journal, June 2011

Characterization and varied expression of a membrane-bound endo-β-1,4-glucanase in hybrid poplar journal, April 2010

Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes journal, July 2011

Efficient Agrobacterium-mediated transformation of commercial hybrid poplar Populus nigra L. × P. maximowiczii A. Henry journal, January 2010

Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice journal, May 2010

Evolution of development of vascular cambia and secondary growth: Tansley review journal, April 2010

Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates journal, April 1991

Generation of transgenic wheat ( Triticum aestivum L.) accumulating heterologous endo-xylanase or ferulic acid esterase in the endosperm journal, April 2010

An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer journal, October 1959

Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance journal, January 2010

Effect of bio-treatment on the lipophilic and hydrophilic extractives of wheat straw journal, June 2009

The Plastochron Index as Applied to Developmental Studies of Cottonwood journal, March 1971

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase journal, June 2017

Grain and cellulosic ethanol: History, economics, and energy policy journal, June 2007

Development of an Efficient Protocol of RNA Isolation from Recalcitrant Tree Tissues journal, August 2007

Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants journal, November 2006

High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome journal, February 2011

The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release journal, November 2017

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan journal, August 1988

Pretreatment: the key to unlocking low-cost cellulosic ethanol journal, January 2008

Expression of a Trichoderma reesei β-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses journal, August 2012

Wood modification by heat treatment: A review journal, December 2008

Engineering hydroxyproline‐ O ‐glycosylated biopolymers to reconstruct the plant cell wall for improved biomass processability journal, January 2020

Global and grain-specific accumulation of glycoside hydrolase family 10 xylanases in transgenic maize (Zea mays): Maize xylanase expression
journal, June 2011

Cell wall modification in tobacco by differential targeting of recombinant endoglucanase from Trichoderma reesei
journal, January 2015

Cell-wall carbohydrates and their modification as a resource for biofuels
journal, May 2008

Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627
journal, November 2007

Growth enhancement of transgenic poplar plants by overexpression of Arabidopsis thaliana endo-1,4- -glucanase (cel1)
journal, January 2004

Biosynthesis of plant cell wall polysaccharides — a complex process
journal, December 2006

Analyzing real-time PCR data by the comparative CT method
journal, June 2008

In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion
journal, September 2017

Expression of a fungal glucuronoyl esterase in Populus: Effects on wood properties and saccharification efficiency
journal, April 2015

Study on crystal structures of enzyme-hydrolyzed cellulosic materials by X-ray diffraction
journal, February 2005

Expression of Glycosyl Hydrolases in Lignocellulosic Feedstock: An Alternative for Affordable Cellulosic Ethanol Production
journal, June 2016

Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development
journal, January 2013

Extremely thermophilic microorganisms for biomass conversion status and prospects
journal, June 2008

Heterologous expression of glycosyl hydrolases in planta: a new departure for biofuels
journal, August 2008

KORRIGAN1 and its Aspen Homolog PttCel9A1 Decrease Cellulose Crystallinity in Arabidopsis Stems
journal, April 2009

RNAi downregulation of three key lignin genes in sugarcane improves glucose release without reduction in sugar production
journal, December 2016

Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility
journal, January 2014

OPTIMIZER: a web server for optimizing the codon usage of DNA sequences
journal, May 2007

In Plant Activation: An Inducible, Hyperexpression Platform for Recombinant Protein Production in Plants
journal, July 2013

Purification, Characterization, and Molecular Analysis of Thermostable Cellulases CelA and CelB fromThermotoga neapolitana
journal, December 1998

Poplar as a feedstock for biofuels: A review of compositional characteristics
journal, March 2010

Recombinant hyperthermophilic enzyme expression in plants: a novel approach for lignocellulose digestion
journal, May 2014

An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants
journal, December 2002

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group
journal, January 1987

Autohydrolysis of plant xylans by apoplastic expression of thermophilic bacterial endo-xylanases
journal, April 2010

Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
journal, March 1959

Significant Increases in Pulping Efficiency in C4H-F5H-Transformed Poplars: Improved Chemical Savings and Reduced Environmental Toxins
journal, October 2003

Feasibility of Large-Scale Biofuel Production
journal, October 1997

In planta expression of A. cellulolyticus Cel5A endocellulase reduces cell wall recalcitrance in tobacco and maize
journal, January 2011

Extreme Thermophilic Enzyme CelB-m Efficiently Degrades the Cellulose in Transgenic Arabidopsis thaliana
journal, July 2015

Hyperthermophilic endoglucanase for in planta lignocellulose conversion
journal, January 2012

Inhibition of enzymatic hydrolysis by residual lignins from softwood-study of enzyme binding and inactivation on lignin-rich surface
journal, June 2011

Characterization and varied expression of a membrane-bound endo-β-1,4-glucanase in hybrid poplar
journal, April 2010

Consolidated Pretreatment and Hydrolysis of Plant Biomass Expressing Cell Wall Degrading Enzymes
journal, July 2011

Efficient Agrobacterium-mediated transformation of commercial hybrid poplar Populus nigra L. × P. maximowiczii A. Henry
journal, January 2010

Stable Production of Thermotolerant Xylanase B of Clostridium stercorarium in Transgenic Tobacco and Rice
journal, May 2010

Evolution of development of vascular cambia and secondary growth: Tansley review
journal, April 2010

Transglycosylation activity of cellobiohydrolase I from Trichoderma longibrachiatum on synthetic and natural substrates
journal, April 1991

Generation of transgenic wheat ( Triticum aestivum L.) accumulating heterologous endo-xylanase or ferulic acid esterase in the endosperm
journal, April 2010

An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray Diffractometer
journal, October 1959

Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance
journal, January 2010

Effect of bio-treatment on the lipophilic and hydrophilic extractives of wheat straw
journal, June 2009

The Plastochron Index as Applied to Developmental Studies of Cottonwood
journal, March 1971

Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase
journal, June 2017

Grain and cellulosic ethanol: History, economics, and energy policy
journal, June 2007

Development of an Efficient Protocol of RNA Isolation from Recalcitrant Tree Tissues
journal, August 2007

Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize plants
journal, November 2006

High-level expression of a suite of thermostable cell wall-degrading enzymes from the chloroplast genome
journal, February 2011

The TcEG1 beetle (Tribolium castaneum) cellulase produced in transgenic switchgrass is active at alkaline pH and auto-hydrolyzes biomass for increased cellobiose release
journal, November 2017

Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan
journal, August 1988

Pretreatment: the key to unlocking low-cost cellulosic ethanol
journal, January 2008

Expression of a Trichoderma reesei β-1,4 endo-xylanase in tall fescue modifies cell wall structure and digestibility and elicits pathogen defence responses
journal, August 2012

Wood modification by heat treatment: A review
journal, December 2008

Engineering hydroxyproline‐ O ‐glycosylated biopolymers to reconstruct the plant cell wall for improved biomass processability
journal, January 2020