skip to main content

DOE PAGESDOE PAGES

Title: Complete enzyme set for chlorophyll biosynthesis in Escherichia coli

Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg–protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in Escherichia coli. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning E. coli cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.
Authors:
ORCiD logo [1] ;  [1] ; ORCiD logo [1] ;  [1] ; ORCiD logo [1] ; ORCiD logo [1] ; ORCiD logo [2] ; ORCiD logo [1]
  1. Univ. of Sheffield (United Kingdom)
  2. Pennsylvania State Univ., University Park, PA (United States)
Publication Date:
Grant/Contract Number:
FG02-94ER20137; SC0001035
Type:
Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Research Org:
Pennsylvania State Univ., University Park, PA (United States); Washington Univ., St. Louis, MO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1499942

Chen, Guangyu E., Canniffe, Daniel P., Barnett, Samuel F. H., Hollingshead, Sarah, Brindley, Amanda A., Vasilev, Cvetelin, Bryant, Donald A., and Hunter, C. Neil. Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. United States: N. p., Web. doi:10.1126/sciadv.aaq1407.
Chen, Guangyu E., Canniffe, Daniel P., Barnett, Samuel F. H., Hollingshead, Sarah, Brindley, Amanda A., Vasilev, Cvetelin, Bryant, Donald A., & Hunter, C. Neil. Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. United States. doi:10.1126/sciadv.aaq1407.
Chen, Guangyu E., Canniffe, Daniel P., Barnett, Samuel F. H., Hollingshead, Sarah, Brindley, Amanda A., Vasilev, Cvetelin, Bryant, Donald A., and Hunter, C. Neil. 2018. "Complete enzyme set for chlorophyll biosynthesis in Escherichia coli". United States. doi:10.1126/sciadv.aaq1407. https://www.osti.gov/servlets/purl/1499942.
@article{osti_1499942,
title = {Complete enzyme set for chlorophyll biosynthesis in Escherichia coli},
author = {Chen, Guangyu E. and Canniffe, Daniel P. and Barnett, Samuel F. H. and Hollingshead, Sarah and Brindley, Amanda A. and Vasilev, Cvetelin and Bryant, Donald A. and Hunter, C. Neil},
abstractNote = {Chlorophylls are essential cofactors for photosynthesis, which sustains global food chains and oxygen production. Billions of tons of chlorophylls are synthesized annually, yet full understanding of chlorophyll biosynthesis has been hindered by the lack of characterization of the Mg–protoporphyrin IX monomethyl ester oxidative cyclase step, which confers the distinctive green color of these pigments. We demonstrate cyclase activity using heterologously expressed enzyme. Next, we assemble a genetic module that encodes the complete chlorophyll biosynthetic pathway and show that it functions in Escherichia coli. Expression of 12 genes converts endogenous protoporphyrin IX into chlorophyll a, turning E. coli cells green. Our results delineate a minimum set of enzymes required to make chlorophyll and establish a platform for engineering photosynthesis in a heterotrophic model organism.},
doi = {10.1126/sciadv.aaq1407},
journal = {Science Advances},
number = 1,
volume = 4,
place = {United States},
year = {2018},
month = {1}
}

Works referenced in this record:

Generic Assignments, Strain Histories and Properties of Pure Cultures of Cyanobacteria
journal, March 1979
  • Stanier, Roger Y.; Deruelles, Josette; Rippka, Rosmarie
  • Microbiology, Vol. 111, Issue 1, p. 1-61
  • DOI: 10.1099/00221287-111-1-1

A Broad Host Range Mobilization System for In Vivo Genetic Engineering Transposon Mutagenesis in Gram Negative Bacteria
journal, November 1983
  • Simon, R.; Priefer, U.; P�hler, A.
  • Bio/Technology, Vol. 1, Issue 9, p. 784-791
  • DOI: 10.1038/nbt1183-784