Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains
Abstract
The spore forming pathogen Bacillus anthracis is the etiologic agent of anthrax in humans and animals. It cycles through infected hosts as vegetative cells and is eventually introduced into the environment where it generates an endospore resistant to many harsh conditions. The endospores are subsequently taken up by another host to begin the next cycle. Outbreaks of anthrax occur regularly worldwide in wildlife and livestock, and the potential for human infection exists whenever humans encounter infected animals. It is also possible to encounter intentional releases of anthrax spores, as was the case in October 2001. Consequently, it is important to be able to rapidly establish the provenance of infectious strains of B. anthracis. Here, we compare protein expression in seven low-passage wild isolates and four laboratory strains of B. anthracis grown under identical conditions using LC-MS/MS proteomic analysis. Of the 1,023 total identified proteins, 96 had significant abundance differences between wild and laboratory strains. Of those, 28 proteins directly related to sporulation were upregulated in wild isolates, with expression driven by Spo0A, CodY, and AbrB/ScoC. In addition, we observed evidence of changes in cell division and fatty acid biosynthesis between the two classes of strains, despite being grown under identicalmore »
- Authors:
-
[1];
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Univ. of Florida, Gainesville, FL (United States)
- Loyola Univ., Chicago, IL (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1497065
- Report Number(s):
- PNNL-SA-137549
Journal ID: ISSN 1932-6203
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 12; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Bacillus anthracis
Citation Formats
Leiser, Owen P., Blackburn, Jason K., Hadfield, Ted L., Kreuzer, Helen W., Wunschel, David S., Bruckner-Lea, Cindy J., and Driks, Adam. Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains. United States: N. p., 2018.
Web. doi:10.1371/journal.pone.0209120.
Leiser, Owen P., Blackburn, Jason K., Hadfield, Ted L., Kreuzer, Helen W., Wunschel, David S., Bruckner-Lea, Cindy J., & Driks, Adam. Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains. United States. https://doi.org/10.1371/journal.pone.0209120
Leiser, Owen P., Blackburn, Jason K., Hadfield, Ted L., Kreuzer, Helen W., Wunschel, David S., Bruckner-Lea, Cindy J., and Driks, Adam. Mon .
"Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains". United States. https://doi.org/10.1371/journal.pone.0209120. https://www.osti.gov/servlets/purl/1497065.
@article{osti_1497065,
title = {Laboratory strains of Bacillus anthracis exhibit pervasive alteration in expression of proteins related to sporulation under laboratory conditions relative to genetically related wild strains},
author = {Leiser, Owen P. and Blackburn, Jason K. and Hadfield, Ted L. and Kreuzer, Helen W. and Wunschel, David S. and Bruckner-Lea, Cindy J. and Driks, Adam},
abstractNote = {The spore forming pathogen Bacillus anthracis is the etiologic agent of anthrax in humans and animals. It cycles through infected hosts as vegetative cells and is eventually introduced into the environment where it generates an endospore resistant to many harsh conditions. The endospores are subsequently taken up by another host to begin the next cycle. Outbreaks of anthrax occur regularly worldwide in wildlife and livestock, and the potential for human infection exists whenever humans encounter infected animals. It is also possible to encounter intentional releases of anthrax spores, as was the case in October 2001. Consequently, it is important to be able to rapidly establish the provenance of infectious strains of B. anthracis. Here, we compare protein expression in seven low-passage wild isolates and four laboratory strains of B. anthracis grown under identical conditions using LC-MS/MS proteomic analysis. Of the 1,023 total identified proteins, 96 had significant abundance differences between wild and laboratory strains. Of those, 28 proteins directly related to sporulation were upregulated in wild isolates, with expression driven by Spo0A, CodY, and AbrB/ScoC. In addition, we observed evidence of changes in cell division and fatty acid biosynthesis between the two classes of strains, despite being grown under identical experimental conditions. These results suggest wild B. anthracis cells are more highly tuned to sporulate than their laboratory cousins, and this difference should be exploited as a method to differentiate between laboratory and low passage wild strains isolated during an anthrax outbreak. This knowledge should distinguish between intentional releases and exposure to strains in nature, providing a basis for the type of response by public health officials and investigators.},
doi = {10.1371/journal.pone.0209120},
journal = {PLoS ONE},
number = 12,
volume = 13,
place = {United States},
year = {Mon Dec 17 00:00:00 EST 2018},
month = {Mon Dec 17 00:00:00 EST 2018}
}
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Figures / Tables:
Fig 1. : Principal coordinate analysis of protein abundance of all proteins identified in this study. Protein abundance values for wild (red) and laboratory (blue) strains of B. anthracis were averaged by strain prior to analysis.
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Works referencing / citing this record:
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Laboratory strains of Bacillus anthracis lose their ability to rapidly grow and sporulate compared to wildlife outbreak strains
journal, January 2020
- Norris, Michael H.; Zincke, Diansy; Leiser, Owen P.
- PLOS ONE, Vol. 15, Issue 1
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