Controlling cell-free metabolism through physiochemical perturbations

Karim, Ashty S.; Heggestad, Jacob T.; Crowe, Samantha A.; Jewett, Michael C.

doi:10.1016/j.ymben.2017.11.005

Title: Controlling cell-free metabolism through physiochemical perturbations

Authors:: Karim, Ashty S.; Heggestad, Jacob T.; Crowe, Samantha A.; Jewett, Michael C.

Publication Date:: Mon Jan 01 00:00:00 EST 2018

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1495880

Grant/Contract Number:: SC0018249

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Metabolic Engineering

Additional Journal Information:: Journal Name: Metabolic Engineering Journal Volume: 45 Journal Issue: C; Journal ID: ISSN 1096-7176

Publisher:: Elsevier

Country of Publication:: Belgium

Language:: English

Citation Formats


                    Karim, Ashty S., Heggestad, Jacob T., Crowe, Samantha A., and Jewett, Michael C. Controlling cell-free metabolism through physiochemical perturbations.  Belgium: N. p., 2018. 
Web.  doi:10.1016/j.ymben.2017.11.005.

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                    Karim, Ashty S., Heggestad, Jacob T., Crowe, Samantha A., & Jewett, Michael C. Controlling cell-free metabolism through physiochemical perturbations.  Belgium.  https://doi.org/10.1016/j.ymben.2017.11.005

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                    Karim, Ashty S., Heggestad, Jacob T., Crowe, Samantha A., and Jewett, Michael C. Mon .  
"Controlling cell-free metabolism through physiochemical perturbations".  Belgium.  https://doi.org/10.1016/j.ymben.2017.11.005.

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@article{osti_1495880,

  title        = {Controlling cell-free metabolism through physiochemical perturbations},

  author       = {Karim, Ashty S. and Heggestad, Jacob T. and Crowe, Samantha A. and Jewett, Michael C.},

  abstractNote = {},

  doi          = {10.1016/j.ymben.2017.11.005},

  journal      = {Metabolic Engineering},

  number       = C,

  volume       = 45,

  place        = {Belgium},

  year         = {Mon Jan 01 00:00:00 EST 2018},

  month        = {Mon Jan 01 00:00:00 EST 2018}

}

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https://doi.org/10.1016/j.ymben.2017.11.005

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Title: Controlling cell-free metabolism through physiochemical perturbations

Citation Formats

Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis journal, March 2011

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Parts plus pipes: Synthetic biology approaches to metabolic engineering journal, May 2012

A synthetic biochemistry module for production of bio-based chemicals from glucose journal, April 2016

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation journal, January 2014

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A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system journal, January 2015

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Synthetic non-oxidative glycolysis enables complete carbon conservation journal, September 2013

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development journal, April 2014

High-throughput preparation methods of crude extract for robust cell-free protein synthesis journal, March 2015

Streptomyces venezuelae TX-TL - a next generation cell-free synthetic biology tool journal, February 2017

Characterizing and prototyping genetic networks with cell-free transcription–translation reactions journal, September 2015

Cell-free metabolic engineering: Biomanufacturing beyond the cell journal, October 2014

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology journal, February 2016

Forward design of a complex enzyme cascade reaction journal, September 2016

A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system journal, July 2017

Synthetic biology and the development of tools for metabolic engineering journal, May 2012

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements journal, November 2016

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery journal, July 2016

Systems metabolic engineering of microorganisms for natural and non-natural chemicals journal, May 2012

Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production journal, October 2011

Construction and optimization of synthetic pathways in metabolic engineering journal, June 2010

The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases journal, November 2013

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis journal, July 2016

Mimicking theEscherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis journal, January 2004

Cell-free protein synthesis energized by slowly-metabolized maltodextrin journal, January 2009

Energizing eukaryotic cell-free protein synthesis with glucose metabolism journal, June 2015

Manufacturing Molecules Through Metabolic Engineering journal, December 2010

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System journal, December 2013

Metabolic engineering of Clostridium tyrobutyricum for n-butanol production through co-utilization of glucose and xylose : Metabolic Engineering of journal, June 2015

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol journal, November 2015

Five hard truths for synthetic biology journal, January 2010

Energizing cell-free protein synthesis with glucose metabolism journal, January 2005

Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source journal, December 2006

Engineering Cellular Metabolism journal, March 2016

In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli : In Vitro Reconstitution of Mevalonate Pathway journal, February 2014

Metabolic engineering of Clostridium acetobutylicum ATCC 824 for the high-yield production of a biofuel composed of an isopropanol/butanol/ethanol mixture journal, July 2013

Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
journal, March 2011

BRENDA in 2015: exciting developments in its 25th year of existence
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Parts plus pipes: Synthetic biology approaches to metabolic engineering
journal, May 2012

A synthetic biochemistry module for production of bio-based chemicals from glucose
journal, April 2016

Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation
journal, January 2014

Synthesis of 2.3 mg/ml of protein with an all Escherichia coli cell-free transcription–translation system
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A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system
journal, January 2015

An integrated cell-free metabolic platform for protein production and synthetic biology
journal, October 2008

Synthetic non-oxidative glycolysis enables complete carbon conservation
journal, September 2013

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development
journal, April 2014

High-throughput preparation methods of crude extract for robust cell-free protein synthesis
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journal, February 2017

Characterizing and prototyping genetic networks with cell-free transcription–translation reactions
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Cell-free metabolic engineering: Biomanufacturing beyond the cell
journal, October 2014

The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology
journal, February 2016

Forward design of a complex enzyme cascade reaction
journal, September 2016

A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system
journal, July 2017

Synthetic biology and the development of tools for metabolic engineering
journal, May 2012

Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements
journal, November 2016

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
journal, July 2016

Systems metabolic engineering of microorganisms for natural and non-natural chemicals
journal, May 2012

Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production
journal, October 2011

Construction and optimization of synthetic pathways in metabolic engineering
journal, June 2010

The MetaCyc database of metabolic pathways and enzymes and the BioCyc collection of Pathway/Genome Databases
journal, November 2013

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis
journal, July 2016

Mimicking theEscherichia coli cytoplasmic environment activates long-lived and efficient cell-free protein synthesis
journal, January 2004

Cell-free protein synthesis energized by slowly-metabolized maltodextrin
journal, January 2009

Energizing eukaryotic cell-free protein synthesis with glucose metabolism
journal, June 2015

Manufacturing Molecules Through Metabolic Engineering
journal, December 2010

Linear DNA for Rapid Prototyping of Synthetic Biological Circuits in an Escherichia coli Based TX-TL Cell-Free System
journal, December 2013

Metabolic engineering of Clostridium tyrobutyricum for n-butanol production through co-utilization of glucose and xylose : Metabolic Engineering of
journal, June 2015

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol
journal, November 2015

Five hard truths for synthetic biology
journal, January 2010

Energizing cell-free protein synthesis with glucose metabolism
journal, January 2005

Cell-free synthesis of proteins that require disulfide bonds using glucose as an energy source
journal, December 2006

Engineering Cellular Metabolism
journal, March 2016

In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli : In Vitro Reconstitution of Mevalonate Pathway
journal, February 2014

Metabolic engineering of Clostridium acetobutylicum ATCC 824 for the high-yield production of a biofuel composed of an isopropanol/butanol/ethanol mixture
journal, July 2013