Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion

Vaynberg, Julia; Fukuda, Koichi; Lu, Fan; Bialkowska, Katarzyna; Chen, Yinghua; Plow, Edward F.; Qin, Jun

doi:10.1038/s41467-018-06906-7

Title: Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion

Abstract

Dynamic communication between integrin-containing complexes (focal adhesions, FAs) and actin filaments is critical for regulating cell adhesion. Pseudokinase ILK plays a key role in this process but the underlying mechanism remains highly elusive. Here in this paper we show that by recruiting FA adaptors PINCH and Parvin into a heterotrimeric complex (IPP), ILK triggers F-actin filament bundling – a process known to generate force/mechanical signal to promote cytoskeleton reassembly and dynamic cell adhesion. Structural, biochemical, and functional analyses revealed that the F-actin bundling is orchestrated by two previously unrecognized WASP-Homology-2 actin binding motifs within IPP, one from PINCH and the other from Parvin. Strikingly, this process is also sensitized to Mg-ATP bound to the pseudoactive site of ILK and its dysregulation severely impairs stress fibers formation, cell spreading, and migration. These data identify a crucial mechanism for ILK, highlighting its uniqueness as a pseudokinase to transduce non-catalytic signal and regulate cell adhesion.

Authors:

Vaynberg, Julia ^[1]; Fukuda, Koichi ^[1]; Lu, Fan ^[2]; Bialkowska, Katarzyna ^[1]; Chen, Yinghua ^[3]; Plow, Edward F. ^[1]; Qin, Jun ^[2]

Cleveland Clinic, Cleveland, OH (United States)
Cleveland Clinic, Cleveland, OH (United States); Case Western Reserve Univ., Cleveland, OH (United States)
Case Western Reserve Univ., Cleveland, OH (United States)

Publication Date:: Fri Oct 26 00:00:00 EDT 2018

Research Org.:: Cleveland Clinic, Cleveland, OH (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1483387

Grant/Contract Number:: AC02-06CH11357; HL058758; 1S10RR026820-01

Resource Type:: Accepted Manuscript

Journal Name:: Nature Communications

Additional Journal Information:: Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Vaynberg, Julia, Fukuda, Koichi, Lu, Fan, Bialkowska, Katarzyna, Chen, Yinghua, Plow, Edward F., and Qin, Jun. Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion.  United States: N. p., 2018. 
Web.  doi:10.1038/s41467-018-06906-7.

Copy to clipboard


                    Vaynberg, Julia, Fukuda, Koichi, Lu, Fan, Bialkowska, Katarzyna, Chen, Yinghua, Plow, Edward F., & Qin, Jun. Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion.  United States.  https://doi.org/10.1038/s41467-018-06906-7

Copy to clipboard


                    Vaynberg, Julia, Fukuda, Koichi, Lu, Fan, Bialkowska, Katarzyna, Chen, Yinghua, Plow, Edward F., and Qin, Jun. Fri .  
"Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion".  United States.  https://doi.org/10.1038/s41467-018-06906-7.  https://www.osti.gov/servlets/purl/1483387.

Copy to clipboard


                    
@article{osti_1483387,

  title        = {Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion},

  author       = {Vaynberg, Julia and Fukuda, Koichi and Lu, Fan and Bialkowska, Katarzyna and Chen, Yinghua and Plow, Edward F. and Qin, Jun},

  abstractNote = {Dynamic communication between integrin-containing complexes (focal adhesions, FAs) and actin filaments is critical for regulating cell adhesion. Pseudokinase ILK plays a key role in this process but the underlying mechanism remains highly elusive. Here in this paper we show that by recruiting FA adaptors PINCH and Parvin into a heterotrimeric complex (IPP), ILK triggers F-actin filament bundling – a process known to generate force/mechanical signal to promote cytoskeleton reassembly and dynamic cell adhesion. Structural, biochemical, and functional analyses revealed that the F-actin bundling is orchestrated by two previously unrecognized WASP-Homology-2 actin binding motifs within IPP, one from PINCH and the other from Parvin. Strikingly, this process is also sensitized to Mg-ATP bound to the pseudoactive site of ILK and its dysregulation severely impairs stress fibers formation, cell spreading, and migration. These data identify a crucial mechanism for ILK, highlighting its uniqueness as a pseudokinase to transduce non-catalytic signal and regulate cell adhesion.},

  doi          = {10.1038/s41467-018-06906-7},

  journal      = {Nature Communications},

  number       = 1,

  volume       = 9,

  place        = {United States},

  year         = {Fri Oct 26 00:00:00 EDT 2018},

  month        = {Fri Oct 26 00:00:00 EDT 2018}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1038/s41467-018-06906-7

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 41 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase
journal, January 1996

Hannigan, Gregory E.; Leung-Hagesteijn, Chungyee; Fitz-Gibbon, Linda
Nature, Vol. 379, Issue 6560
DOI: 10.1038/379091a0

C. elegans PAT-4/ILK Functions as an Adaptor Protein within Integrin Adhesion Complexes
journal, May 2002

Mackinnon, A. Craig; Qadota, Hiroshi; Norman, Kenneth R.
Current Biology, Vol. 12, Issue 10
DOI: 10.1016/S0960-9822(02)00810-2

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation
journal, April 2003

Sakai, T.
Genes & Development, Vol. 17, Issue 7
DOI: 10.1101/gad.255603

Emerging roles of pseudokinases
journal, September 2006

Boudeau, Jérôme; Miranda-Saavedra, Diego; Barton, Geoffrey J.
Trends in Cell Biology, Vol. 16, Issue 9
DOI: 10.1016/j.tcb.2006.07.003

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

Williams, Ag; Woolkalis, Mj; Poncz, M.
Blood, Vol. 76, Issue 4
DOI: 10.1182/blood.v76.4.721.bloodjournal764721

The Structure of α-Parvin CH2-Paxillin LD1 Complex Reveals a Novel Modular Recognition for Focal Adhesion Assembly
journal, May 2008

Wang, Xiaoxia; Fukuda, Koichi; Byeon, In-Ja
Journal of Biological Chemistry, Vol. 283, Issue 30
DOI: 10.1074/jbc.m801270200

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane
journal, March 2001

Zervas, Christos G.; Gregory, Stephen L.; Brown, Nicholas H.
The Journal of Cell Biology, Vol. 152, Issue 5
DOI: 10.1083/jcb.152.5.1007

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites
journal, December 2002

Zhang, Y.
Journal of Cell Science, Vol. 115, Issue 24
DOI: 10.1242/jcs.00166

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch
journal, June 2015

Case, Lindsay B.; Waterman, Clare M.
Nature Cell Biology, Vol. 17, Issue 8
DOI: 10.1038/ncb3191

Spire and Cordon-bleu: multifunctional regulators of actin dynamics
journal, October 2008

Renault, Louis; Bugyi, Beáta; Carlier, Marie-France
Trends in Cell Biology, Vol. 18, Issue 10
DOI: 10.1016/j.tcb.2008.07.008

The integrin adhesome: from genes and proteins to human disease
journal, March 2014

Winograd-Katz, Sabina E.; Fässler, Reinhard; Geiger, Benjamin
Nature Reviews Molecular Cell Biology, Vol. 15, Issue 4
DOI: 10.1038/nrm3769

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase!
journal, December 2009

Wickström, Sara A.; Lange, Anika; Montanez, Eloi
The EMBO Journal, Vol. 29, Issue 2
DOI: 10.1038/emboj.2009.376

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation
journal, November 2009

Zeqiraj, E.; Filippi, B. M.; Deak, M.
Science, Vol. 326, Issue 5960
DOI: 10.1126/science.1178377

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling
journal, October 2012

Qin, Jun; Wu, Chuanyue
Current Opinion in Cell Biology, Vol. 24, Issue 5
DOI: 10.1016/j.ceb.2012.06.003

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells ^†
journal, September 2004

Joel, Peteranne B.; Fagnant, Patricia M.; Trybus, Kathleen M.
Biochemistry, Vol. 43, Issue 36
DOI: 10.1021/bi048899a

Integrin-linked kinase is an adaptor with essential functions during mouse development
journal, October 2009

Lange, Anika; Wickström, Sara A.; Jakobson, Madis
Nature, Vol. 461, Issue 7266
DOI: 10.1038/nature08468

ILK, PINCH and parvin: the tIPP of integrin signalling
journal, December 2005

Legate, Kyle R.; Montañez, Eloi; Kudlacek, Oliver
Nature Reviews Molecular Cell Biology, Vol. 7, Issue 1
DOI: 10.1038/nrm1789

Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif
journal, August 2015

Park, Eunyoung; Graziano, Brian R.; Zheng, Wei
Structure, Vol. 23, Issue 8
DOI: 10.1016/j.str.2015.05.015

Structure of an Ultraweak Protein-Protein Complex and Its Crucial Role in Regulation of Cell Morphology and Motility
journal, February 2005

Vaynberg, Julia; Fukuda, Tomohiko; Chen, Ka
Molecular Cell, Vol. 17, Issue 4
DOI: 10.1016/j.molcel.2004.12.031

A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams
journal, December 2011

Garrett, Daniel S.; Powers, Robert; Gronenborn, Angela M.
Journal of Magnetic Resonance, Vol. 213, Issue 2
DOI: 10.1016/j.jmr.2011.09.007

Determining the structures of large proteins and protein complexes by NMR
journal, January 1998

Clore, G. Marius; Gronenborn, Angela M.
Trends in Biotechnology, Vol. 16, Issue 1
DOI: 10.1016/s0167-7799(97)01135-9

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex
journal, May 2011

Rooney, Nicholas; Streuli, Charles H.
FEBS Letters, Vol. 585, Issue 11
DOI: 10.1016/j.febslet.2011.05.014

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
journal, January 2017

Liang, Xiquan; Potter, Jason; Kumar, Shantanu
Journal of Biotechnology, Vol. 241
DOI: 10.1016/j.jbiotec.2016.11.011

Mechanotransduction and extracellular matrix homeostasis
journal, October 2014

Humphrey, Jay D.; Dufresne, Eric R.; Schwartz, Martin A.
Nature Reviews Molecular Cell Biology, Vol. 15, Issue 12
DOI: 10.1038/nrm3896

Structural Basis of Focal Adhesion Localization of LIM-only Adaptor PINCH by Integrin-linked Kinase
journal, December 2008

Yang, Yanwu; Wang, Xiaoxia; Hawkins, Cheryl A.
Journal of Biological Chemistry, Vol. 284, Issue 9
DOI: 10.1074/jbc.m805319200

Structure and function of focal adhesions
journal, February 2012

Wehrle-Haller, Bernhard
Current Opinion in Cell Biology, Vol. 24, Issue 1
DOI: 10.1016/j.ceb.2011.11.001

HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes
journal, July 2006

Minor, Wladek; Cymborowski, Marcin; Otwinowski, Zbyszek
Acta Crystallographica Section D Biological Crystallography, Vol. 62, Issue 8
DOI: 10.1107/S0907444906019949

Chemical shift assignments of the catalytic and ATP-binding domain of HK853 from Thermotoga maritime
journal, January 2019

Zhou, Yuan; Liu, Xinghong; Li, Conggang
Biomolecular NMR Assignments, Vol. 13, Issue 1
DOI: 10.1007/s12104-019-09872-3

Structure and function of focal adhesions
journal, February 2012

Wehrle-Haller, Bernhard
Current Opinion in Cell Biology, Vol. 24, Issue 1
DOI: 10.1016/j.ceb.2011.11.001

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly
journal, November 2005

Chereau, D.; Kerff, F.; Graceffa, P.
Proceedings of the National Academy of Sciences, Vol. 102, Issue 46
DOI: 10.1073/pnas.0507021102

Purification and SAXS Analysis of the Integrin Linked Kinase, PINCH, Parvin (IPP) Heterotrimeric Complex
journal, January 2013

Stiegler, Amy L.; Grant, Thomas D.; Luft, Joseph R.
PLoS ONE, Vol. 8, Issue 1
DOI: 10.1371/journal.pone.0055591

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling
journal, October 2012

Qin, Jun; Wu, Chuanyue
Current Opinion in Cell Biology, Vol. 24, Issue 5
DOI: 10.1016/j.ceb.2012.06.003

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

Murshudov, G. N.; Vagin, A. A.; Dodson, E. J.
Acta Crystallographica Section D Biological Crystallography, Vol. 53, Issue 3
DOI: 10.1107/s0907444996012255

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1
journal, August 2004

Stevens, J. M.; Jordan, P. A.; Sage, T.
Journal of Thrombosis and Haemostasis, Vol. 2, Issue 8
DOI: 10.1111/j.1538-7836.2004.00870.x

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

Delaglio, Frank; Grzesiek, Stephan; Vuister, GeertenW.
Journal of Biomolecular NMR, Vol. 6, Issue 3
DOI: 10.1007/BF00197809

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
journal, February 2001

Tan, Song
Protein Expression and Purification, Vol. 21, Issue 1
DOI: 10.1006/prep.2000.1363

Biochemical, Proteomic, Structural, and Thermodynamic Characterizations of Integrin-linked Kinase (ILK): CROSS-VALIDATION OF THE PSEUDOKINASE
journal, April 2011

Fukuda, Koichi; Knight, James D. R.; Piszczek, Grzegorz
Journal of Biological Chemistry, Vol. 286, Issue 24
DOI: 10.1074/jbc.M111.240093

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch
journal, June 2015

Case, Lindsay B.; Waterman, Clare M.
Nature Cell Biology, Vol. 17, Issue 8
DOI: 10.1038/ncb3191

The secret life of kinases: insights into non-catalytic signalling functions from pseudokinases
journal, June 2017

Jacobsen, Annette V.; Murphy, James M.
Biochemical Society Transactions, Vol. 45, Issue 3
DOI: 10.1042/bst20160331

Integrin-linked kinase: a cancer therapeutic target unique among its ILK
journal, January 2005

Hannigan, Gregory; Troussard, Armelle A.; Dedhar, Shoukat
Nature Reviews Cancer, Vol. 5, Issue 1
DOI: 10.1038/nrc1524

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex
journal, June 2008

Postel, Ruben; Vakeel, Padmanabhan; Topczewski, Jacek
Developmental Biology, Vol. 318, Issue 1
DOI: 10.1016/j.ydbio.2008.03.024

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
journal, March 2006

Loomis, Patricia A.; Kelly, Alexander E.; Zheng, Lili
Journal of Cell Science, Vol. 119, Issue 8
DOI: 10.1242/jcs.02869

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase
journal, January 1996

Hannigan, Gregory E.; Leung-Hagesteijn, Chungyee; Fitz-Gibbon, Linda
Nature, Vol. 379, Issue 6560
DOI: 10.1038/379091a0

Mechanotransduction and extracellular matrix homeostasis
journal, October 2014

Humphrey, Jay D.; Dufresne, Eric R.; Schwartz, Martin A.
Nature Reviews Molecular Cell Biology, Vol. 15, Issue 12
DOI: 10.1038/nrm3896

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation
journal, April 2003

Sakai, T.
Genes & Development, Vol. 17, Issue 7
DOI: 10.1101/gad.255603

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

Williams, Ag; Woolkalis, Mj; Poncz, M.
Blood, Vol. 76, Issue 4
DOI: 10.1182/blood.v76.4.721.bloodjournal764721

Integrin Adhesion: When Is a Kinase a Kinase?
journal, May 2002

Zervas, Christos G.; Brown, Nicholas H.
Current Biology, Vol. 12, Issue 10
DOI: 10.1016/s0960-9822(02)00856-4

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation
journal, November 2009

Zeqiraj, E.; Filippi, B. M.; Deak, M.
Science, Vol. 326, Issue 5960
DOI: 10.1126/science.1178377

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
journal, May 2014

Montague, Tessa G.; Cruz, José M.; Gagnon, James A.
Nucleic Acids Research, Vol. 42, Issue W1
DOI: 10.1093/nar/gku410

Integrin signalling adaptors: not only figurants in the cancer story
journal, November 2010

Cabodi, Sara; del Pilar Camacho-Leal, Maria; Di Stefano, Paola
Nature Reviews Cancer, Vol. 10, Issue 12
DOI: 10.1038/nrc2967

The oligomeric structures of plant cryptochromes
journal, May 2020

Shao, Kai; Zhang, Xue; Li, Xu
Nature Structural & Molecular Biology, Vol. 27, Issue 5
DOI: 10.1038/s41594-020-0420-x

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane
journal, March 2001

Zervas, Christos G.; Gregory, Stephen L.; Brown, Nicholas H.
The Journal of Cell Biology, Vol. 152, Issue 5
DOI: 10.1083/jcb.152.5.1007

Functional plasticity of CH domains
journal, December 2001

Gimona, Mario; Djinovic-Carugo, Kristina; Kranewitter, Wolfgang J.
FEBS Letters, Vol. 513, Issue 1
DOI: 10.1016/S0014-5793(01)03240-9

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites
journal, December 2002

Zhang, Y.
Journal of Cell Science, Vol. 115, Issue 24
DOI: 10.1242/jcs.00166

ILK, PINCH and parvin: the tIPP of integrin signalling
journal, December 2005

Legate, Kyle R.; Montañez, Eloi; Kudlacek, Oliver
Nature Reviews Molecular Cell Biology, Vol. 7, Issue 1
DOI: 10.1038/nrm1789

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex
journal, May 2011

Rooney, Nicholas; Streuli, Charles H.
FEBS Letters, Vol. 585, Issue 11
DOI: 10.1016/j.febslet.2011.05.014

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

Williams, Ag; Woolkalis, Mj; Poncz, M.
Blood, Vol. 76, Issue 4
DOI: 10.1182/blood.V76.4.721.721

Integrin-linked kinase is an adaptor with essential functions during mouse development
journal, October 2009

Lange, Anika; Wickström, Sara A.; Jakobson, Madis
Nature, Vol. 461, Issue 7266
DOI: 10.1038/nature08468

PROCHECK: a program to check the stereochemical quality of protein structures
journal, April 1993

Laskowski, R. A.; MacArthur, M. W.; Moss, D. S.
Journal of Applied Crystallography, Vol. 26, Issue 2
DOI: 10.1107/S0021889892009944

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
journal, March 2006

Loomis, Patricia A.; Kelly, Alexander E.; Zheng, Lili
Journal of Cell Science, Vol. 119, Issue 8
DOI: 10.1242/jcs.02869

The structural basis of integrin-linked kinase-PINCH interactions
journal, December 2008

Chiswell, B. P.; Zhang, R.; Murphy, J. W.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 52
DOI: 10.1073/pnas.0811415106

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells ^†
journal, September 2004

Joel, Peteranne B.; Fagnant, Patricia M.; Trybus, Kathleen M.
Biochemistry, Vol. 43, Issue 36
DOI: 10.1021/bi048899a

The integrin adhesome: from genes and proteins to human disease
journal, March 2014

Winograd-Katz, Sabina E.; Fässler, Reinhard; Geiger, Benjamin
Nature Reviews Molecular Cell Biology, Vol. 15, Issue 4
DOI: 10.1038/nrm3769

Integrin-linked kinase: a cancer therapeutic target unique among its ILK
journal, January 2005

Hannigan, Gregory; Troussard, Armelle A.; Dedhar, Shoukat
Nature Reviews Cancer, Vol. 5, Issue 1
DOI: 10.1038/nrc1524

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions
journal, December 2009

Fukuda, Koichi; Gupta, Sudhiranjan; Chen, Ka
Molecular Cell, Vol. 36, Issue 5
DOI: 10.1016/j.molcel.2009.11.028

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase!
journal, December 2009

Wickström, Sara A.; Lange, Anika; Montanez, Eloi
The EMBO Journal, Vol. 29, Issue 2
DOI: 10.1038/emboj.2009.376

Emerging roles of pseudokinases
journal, September 2006

Boudeau, Jérôme; Miranda-Saavedra, Diego; Barton, Geoffrey J.
Trends in Cell Biology, Vol. 16, Issue 9
DOI: 10.1016/j.tcb.2006.07.003

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
journal, February 2001

Tan, Song
Protein Expression and Purification, Vol. 21, Issue 1
DOI: 10.1006/prep.2000.1363

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1
journal, August 2004

Stevens, J. M.; Jordan, P. A.; Sage, T.
Journal of Thrombosis and Haemostasis, Vol. 2, Issue 8
DOI: 10.1111/j.1538-7836.2004.00870.x

Spire and Cordon-bleu: multifunctional regulators of actin dynamics
journal, October 2008

Renault, Louis; Bugyi, Beáta; Carlier, Marie-France
Trends in Cell Biology, Vol. 18, Issue 10
DOI: 10.1016/j.tcb.2008.07.008

The WH2 Domain and Actin Nucleation: Necessary but Insufficient
journal, June 2016

Dominguez, Roberto
Trends in Biochemical Sciences, Vol. 41, Issue 6
DOI: 10.1016/j.tibs.2016.03.004

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
journal, January 2017

Liang, Xiquan; Potter, Jason; Kumar, Shantanu
Journal of Biotechnology, Vol. 241
DOI: 10.1016/j.jbiotec.2016.11.011

Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties
journal, April 2007

Millard, T. H.; Dawson, J.; Machesky, L. M.
Journal of Cell Science, Vol. 120, Issue 9
DOI: 10.1242/jcs.001776

TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts
journal, June 2009

Shen, Yang; Delaglio, Frank; Cornilescu, Gabriel
Journal of Biomolecular NMR, Vol. 44, Issue 4
DOI: 10.1007/s10858-009-9333-z

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex
journal, June 2008

Postel, Ruben; Vakeel, Padmanabhan; Topczewski, Jacek
Developmental Biology, Vol. 318, Issue 1
DOI: 10.1016/j.ydbio.2008.03.024

Solution Structure of the Focal Adhesion Adaptor PINCH LIM1 Domain and Characterization of Its Interaction with the Integrin-linked Kinase Ankyrin Repeat Domain
journal, November 2000

Velyvis, Algirdas; Yang, Yanwu; Wu, Chuanyue
Journal of Biological Chemistry, Vol. 276, Issue 7
DOI: 10.1074/jbc.m007632200

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions
journal, December 2009

Fukuda, Koichi; Gupta, Sudhiranjan; Chen, Ka
Molecular Cell, Vol. 36, Issue 5
DOI: 10.1016/j.molcel.2009.11.028

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly
journal, November 2005

Chereau, D.; Kerff, F.; Graceffa, P.
Proceedings of the National Academy of Sciences, Vol. 102, Issue 46
DOI: 10.1073/pnas.0507021102

The Xplor-NIH NMR molecular structure determination package
journal, January 2003

Schwieters, Charles D.; Kuszewski, John J.; Tjandra, Nico
Journal of Magnetic Resonance, Vol. 160, Issue 1
DOI: 10.1016/s1090-7807(02)00014-9

The structural basis of integrin-linked kinase-PINCH interactions
journal, December 2008

Chiswell, B. P.; Zhang, R.; Murphy, J. W.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 52
DOI: 10.1073/pnas.0811415106

MOLREP an Automated Program for Molecular Replacement
journal, December 1997

Vagin, A.; Teplyakov, A.
Journal of Applied Crystallography, Vol. 30, Issue 6, p. 1022-1025
DOI: 10.1107/s0021889897006766

Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif
journal, August 2015

Park, Eunyoung; Graziano, Brian R.; Zheng, Wei
Structure, Vol. 23, Issue 8
DOI: 10.1016/j.str.2015.05.015

A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams
journal, October 1991

Garrett, Daniel S.; Powers, Robert; Gronenborn, Angela M.
Journal of Magnetic Resonance (1969), Vol. 95, Issue 1
DOI: 10.1016/0022-2364(91)90341-p

Multi-functionality of a tryptophan residue conserved in substrate-binding groove of GH19 chitinases
journal, January 2021

Nagata, Takuya; Shinya, Shoko; Ohnuma, Takayuki
Scientific Reports, Vol. 11, Issue 1
DOI: 10.1038/s41598-021-81903-3

Receptor and G protein-mediated responses to thrombin in HEL cells.
journal, January 1991

Brass, L. F.; Manning, D. R.; Williams, A. G.
Journal of Biological Chemistry, Vol. 266, Issue 2
DOI: 10.1016/S0021-9258(17)35267-5

Works referencing / citing this record:

Cell Adhesion by Integrins
journal, October 2019

Bachmann, Michael; Kukkurainen, Sampo; Hytönen, Vesa P.
Physiological Reviews, Vol. 99, Issue 4
DOI: 10.1152/physrev.00036.2018

Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
journal, December 2018

Urner, Sofia; Planas‐Paz, Lara; Hilger, Laura Sophie
The EMBO Journal, Vol. 38, Issue 2
DOI: 10.15252/embj.201899322

Genetic Animal Models for Arrhythmogenic Cardiomyopathy
journal, June 2020

Gerull, Brenda; Brodehl, Andreas
Frontiers in Physiology, Vol. 11
DOI: 10.3389/fphys.2020.00624

Identification of ILK as a critical regulator of VEGFR 3 signalling and lymphatic vascular growth
journal, December 2018

Urner, Sofia; Planas‐Paz, Lara; Hilger, Laura Sophie
The EMBO Journal, Vol. 38, Issue 2
DOI: 10.15252/embj.201899322

ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process
journal, March 2020

Gagné, David; Benoit, Yannick D.; Groulx, Jean-François
BMC Molecular and Cell Biology, Vol. 21, Issue 1
DOI: 10.1186/s12860-020-00259-0

ILK supports RhoA/ROCK-mediated contractility of human intestinal epithelial crypt cells by inducing the fibrillogenesis of endogenous soluble fibronectin during the spreading process
journal, March 2020

Gagné, David; Benoit, Yannick D.; Groulx, Jean-François
BMC Molecular and Cell Biology, Vol. 21, Issue 1
DOI: 10.1186/s12860-020-00259-0

Emerging regulators of vascular smooth muscle cell migration
journal, June 2019

Afewerki, TecLino; Ahmed, Sultan; Warren, Derek
Journal of Muscle Research and Cell Motility, Vol. 40, Issue 2
DOI: 10.1007/s10974-019-09531-z

Similar Records in DOE PAGES and OSTI.GOV collections:

Structural Basis of Focal Adhesion Localization of LIM-only Adaptor PINCH by Integrin-linked Kinase

Journal Article Yang, Yanwu ; Wang, Xiaoxia ; Hawkins, Cheryl A ; ... - J. Biol. Chem.

The LIM-only adaptor PINCH (the particularly interesting cysteine- and histidine-rich protein) plays a pivotal role in the assembly of focal adhesions (FAs), supramolecular complexes that transmit mechanical and biochemical information between extracellular matrix and actin cytoskeleton, regulating diverse cell adhesive processes such as cell migration, cell spreading, and survival. A key step for the PINCH function is its localization to FAs, which depends critically on the tight binding of PINCH to integrin-linked kinase (ILK). Here we report the solution NMR structure of the core ILK {center_dot} PINCH complex (28 kDa, K{sub D} {approx} 68 nm) involving the N-terminal ankyrin repeatmore »« less
https://doi.org/10.1074/jbc.M805319200
Structural Basis of Competition Between PINCH1 and PINCH2 for Binding to the Ankyrin Repeat Domain of Integrin-linked Kinase

Journal Article Chiswell, B ; Steigler, A ; Razinia, Z ; ... - Journal of Structural Biology

Formation of a heterotrimeric IPP complex composed of integrin-linked kinase (ILK), the LIM domain protein PINCH, and parvin is important for signaling through integrin adhesion receptors. Mammals possess two PINCH genes that are expressed simultaneously in many tissues. PINCH1 and PINCH2 have overlapping functions and can compensate for one another in many settings; however, isoform-specific functions have been reported and it is proposed that association with a PINCH1- or PINCH2-containing IPP complex may provide a bifurcation point in integrin signaling promoting different cellular responses. Here we report that the LIM1 domains of PINCH1 and PINCH2 directly compete for the samemore »« less
https://doi.org/10.1016/j.jsb.2009.12.002
Purification and SAXS Analysis of the Integrin Linked Kinase, PINCH, Parvin (IPP) Heterotrimeric Complex

Journal Article Stiegler, Amy L. ; Grant, Thomas D. ; Luft, Joseph R. ; ... - PLoS ONE

The heterotrimeric protein complex containing the integrin linked kinase (ILK), parvin, and PINCH proteins, termed the IPP complex, is an essential component of focal adhesions, where it interacts with many proteins to mediate signaling from integrin adhesion receptors. Here we conduct a biochemical and structural analysis of the minimal IPP complex, comprising full-length human ILK, the LIM1 domain of PINCH1, and the CH2 domain of α-parvin. We provide a detailed purification protocol for IPP and show that the purified IPP complex is stable and monodisperse in solution. Using small-angle X-ray scattering (SAXS), we also conduct the first structural characterization ofmore »« less
https://doi.org/10.1371/journal.pone.0055591

Full Text Available
Integrin-linked kinase is involved in matrix-induced hepatocyte differentiation

Journal Article Gkretsi, Vasiliki ; Bowen, William C ; Yang, Yu ; ... - Biochemical and Biophysical Research Communications

Hepatocytes have restricted proliferative capacity in culture and when cultured without matrix, lose the hepatocyte-specific gene expression and characteristic cellular micro-architecture. Overlay of matrix-preparations on de-differentiated hepatocytes restores differentiation. Integrin-linked kinase (ILK) is a cell-matrix-adhesion protein crucial in fundamental processes such as differentiation and survival. In this study, we investigated the role of ILK, and its binding partners PINCH, {alpha}-parvin, and Mig-2 in matrix-induced hepatocyte differentiation. We report here that ILK is present in the liver and localizes at cell-matrix adhesions of cultured hepatocytes. We also show that ILK, PINCH, {alpha}-parvin, and Mig-2 expression level is dramatically reduced in themore »« less
https://doi.org/10.1016/j.bbrc.2006.12.091
Thymosin β4 induces invasion and migration of human colorectal cancer cells through the ILK/AKT/β-catenin signaling pathway

Journal Article Piao, Zhengri ; Center for Creative Biomedical Scientists ; Hong, Chang-Soo ; ... - Biochemical and Biophysical Research Communications

Highlights: • Tβ4 is overexpressed in human colorectal cancer cells. • The overexpression of Tβ4 is correlated with stage of colorectal cancer. • Tβ4 stimulates cell adhesion, invasion, migration and EMT. • Tβ4 activates the ILK/AKT/β-catenin signaling pathway. - Abstract: Thymosin β4 (Tβ4) is a 43-amino-acid peptide involved in many biological processes. However, the precise molecular signaling mechanism(s) of Tβ4 in cell invasion and migration remain unclear. In this study, we show that Tβ4 was significantly overexpressed in colorectal cancer tissues compared to adjacent normal tissues and high levels of Tβ4 were correlated with stage of colorectal cancer, and thatmore »« less
https://doi.org/10.1016/J.BBRC.2014.09.012

Similar Records
Related Works
dataset (1)

Title: Non-catalytic signaling by pseudokinase ILK for regulating cell adhesion

Abstract

Citation Formats

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase journal, January 1996

C. elegans PAT-4/ILK Functions as an Adaptor Protein within Integrin Adhesion Complexes journal, May 2002

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation journal, April 2003

Emerging roles of pseudokinases journal, September 2006

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells journal, August 1990

The Structure of α-Parvin CH2-Paxillin LD1 Complex Reveals a Novel Modular Recognition for Focal Adhesion Assembly journal, May 2008

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane journal, March 2001

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites journal, December 2002

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch journal, June 2015

Spire and Cordon-bleu: multifunctional regulators of actin dynamics journal, October 2008

The integrin adhesome: from genes and proteins to human disease journal, March 2014

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase! journal, December 2009

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation journal, November 2009

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling journal, October 2012

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells † journal, September 2004

Integrin-linked kinase is an adaptor with essential functions during mouse development journal, October 2009

ILK, PINCH and parvin: the tIPP of integrin signalling journal, December 2005

Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif journal, August 2015

Structure of an Ultraweak Protein-Protein Complex and Its Crucial Role in Regulation of Cell Morphology and Motility journal, February 2005

A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams journal, December 2011

Determining the structures of large proteins and protein complexes by NMR journal, January 1998

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex journal, May 2011

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA journal, January 2017

Mechanotransduction and extracellular matrix homeostasis journal, October 2014

Structural Basis of Focal Adhesion Localization of LIM-only Adaptor PINCH by Integrin-linked Kinase journal, December 2008

Structure and function of focal adhesions journal, February 2012

HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes journal, July 2006

Chemical shift assignments of the catalytic and ATP-binding domain of HK853 from Thermotoga maritime journal, January 2019

Structure and function of focal adhesions journal, February 2012

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly journal, November 2005

Purification and SAXS Analysis of the Integrin Linked Kinase, PINCH, Parvin (IPP) Heterotrimeric Complex journal, January 2013

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling journal, October 2012

Refinement of Macromolecular Structures by the Maximum-Likelihood Method journal, May 1997

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1 journal, August 2004

NMRPipe: A multidimensional spectral processing system based on UNIX pipes journal, November 1995

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli journal, February 2001

Biochemical, Proteomic, Structural, and Thermodynamic Characterizations of Integrin-linked Kinase (ILK): CROSS-VALIDATION OF THE PSEUDOKINASE journal, April 2011

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch journal, June 2015

The secret life of kinases: insights into non-catalytic signalling functions from pseudokinases journal, June 2017

Integrin-linked kinase: a cancer therapeutic target unique among its ILK journal, January 2005

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex journal, June 2008

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells journal, March 2006

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase journal, January 1996

Mechanotransduction and extracellular matrix homeostasis journal, October 2014

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation journal, April 2003

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells journal, August 1990

Integrin Adhesion: When Is a Kinase a Kinase? journal, May 2002

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation journal, November 2009

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing journal, May 2014

Integrin signalling adaptors: not only figurants in the cancer story journal, November 2010

The oligomeric structures of plant cryptochromes journal, May 2020

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane journal, March 2001

Functional plasticity of CH domains journal, December 2001

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites journal, December 2002

ILK, PINCH and parvin: the tIPP of integrin signalling journal, December 2005

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex journal, May 2011

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells journal, August 1990

Integrin-linked kinase is an adaptor with essential functions during mouse development journal, October 2009

PROCHECK: a program to check the stereochemical quality of protein structures journal, April 1993

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells journal, March 2006

The structural basis of integrin-linked kinase-PINCH interactions journal, December 2008

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells † journal, September 2004

The integrin adhesome: from genes and proteins to human disease journal, March 2014

Integrin-linked kinase: a cancer therapeutic target unique among its ILK journal, January 2005

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions journal, December 2009

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase! journal, December 2009

Emerging roles of pseudokinases journal, September 2006

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli journal, February 2001

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1 journal, August 2004

Spire and Cordon-bleu: multifunctional regulators of actin dynamics journal, October 2008

The WH2 Domain and Actin Nucleation: Necessary but Insufficient journal, June 2016

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA journal, January 2017

Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties journal, April 2007

TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts journal, June 2009

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex journal, June 2008

Solution Structure of the Focal Adhesion Adaptor PINCH LIM1 Domain and Characterization of Its Interaction with the Integrin-linked Kinase Ankyrin Repeat Domain journal, November 2000

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions journal, December 2009

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase
journal, January 1996

C. elegans PAT-4/ILK Functions as an Adaptor Protein within Integrin Adhesion Complexes
journal, May 2002

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation
journal, April 2003

Emerging roles of pseudokinases
journal, September 2006

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

The Structure of α-Parvin CH2-Paxillin LD1 Complex Reveals a Novel Modular Recognition for Focal Adhesion Assembly
journal, May 2008

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane
journal, March 2001

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites
journal, December 2002

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch
journal, June 2015

Spire and Cordon-bleu: multifunctional regulators of actin dynamics
journal, October 2008

The integrin adhesome: from genes and proteins to human disease
journal, March 2014

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase!
journal, December 2009

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation
journal, November 2009

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling
journal, October 2012

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells ^†
journal, September 2004

Integrin-linked kinase is an adaptor with essential functions during mouse development
journal, October 2009

ILK, PINCH and parvin: the tIPP of integrin signalling
journal, December 2005

Structure of a Bud6/Actin Complex Reveals a Novel WH2-like Actin Monomer Recruitment Motif
journal, August 2015

Structure of an Ultraweak Protein-Protein Complex and Its Crucial Role in Regulation of Cell Morphology and Motility
journal, February 2005

A common sense approach to peak picking in two-, three-, and four-dimensional spectra using automatic computer analysis of contour diagrams
journal, December 2011

Determining the structures of large proteins and protein complexes by NMR
journal, January 1998

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex
journal, May 2011

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
journal, January 2017

Mechanotransduction and extracellular matrix homeostasis
journal, October 2014

Structural Basis of Focal Adhesion Localization of LIM-only Adaptor PINCH by Integrin-linked Kinase
journal, December 2008

Structure and function of focal adhesions
journal, February 2012

HKL -3000: the integration of data reduction and structure solution – from diffraction images to an initial model in minutes
journal, July 2006

Chemical shift assignments of the catalytic and ATP-binding domain of HK853 from Thermotoga maritime
journal, January 2019

Structure and function of focal adhesions
journal, February 2012

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly
journal, November 2005

Purification and SAXS Analysis of the Integrin Linked Kinase, PINCH, Parvin (IPP) Heterotrimeric Complex
journal, January 2013

ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling
journal, October 2012

Refinement of Macromolecular Structures by the Maximum-Likelihood Method
journal, May 1997

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1
journal, August 2004

NMRPipe: A multidimensional spectral processing system based on UNIX pipes
journal, November 1995

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
journal, February 2001

Biochemical, Proteomic, Structural, and Thermodynamic Characterizations of Integrin-linked Kinase (ILK): CROSS-VALIDATION OF THE PSEUDOKINASE
journal, April 2011

Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch
journal, June 2015

The secret life of kinases: insights into non-catalytic signalling functions from pseudokinases
journal, June 2017

Integrin-linked kinase: a cancer therapeutic target unique among its ILK
journal, January 2005

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex
journal, June 2008

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
journal, March 2006

Regulation of cell adhesion and anchorage-dependent growth by a new β1-integrin-linked protein kinase
journal, January 1996

Mechanotransduction and extracellular matrix homeostasis
journal, October 2014

Integrin-linked kinase (ILK) is required for polarizing the epiblast, cell adhesion, and controlling actin accumulation
journal, April 2003

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

Integrin Adhesion: When Is a Kinase a Kinase?
journal, May 2002

Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation
journal, November 2009

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing
journal, May 2014

Integrin signalling adaptors: not only figurants in the cancer story
journal, November 2010

The oligomeric structures of plant cryptochromes
journal, May 2020

Drosophila Integrin-Linked Kinase Is Required at Sites of Integrin Adhesion to Link the Cytoskeleton to the Plasma Membrane
journal, March 2001

Functional plasticity of CH domains
journal, December 2001

Assembly of the PINCH-ILK-CH-ILKBP complex precedes and is essential for localization of each component to cell-matrix adhesion sites
journal, December 2002

ILK, PINCH and parvin: the tIPP of integrin signalling
journal, December 2005

How integrins control mammary epithelial differentiation: A possible role for the ILK-PINCH-Parvin complex
journal, May 2011

Identification of the pertussis toxin-sensitive G proteins in platelets, megakaryocytes, and human erythroleukemia cells
journal, August 1990

Integrin-linked kinase is an adaptor with essential functions during mouse development
journal, October 2009

PROCHECK: a program to check the stereochemical quality of protein structures
journal, April 1993

Targeted wild-type and jerker espins reveal a novel, WH2-domain-dependent way to make actin bundles in cells
journal, March 2006

The structural basis of integrin-linked kinase-PINCH interactions
journal, December 2008

Expression of a Nonpolymerizable Actin Mutant in Sf9 Cells ^†
journal, September 2004

The integrin adhesome: from genes and proteins to human disease
journal, March 2014

Integrin-linked kinase: a cancer therapeutic target unique among its ILK
journal, January 2005

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions
journal, December 2009

The ILK/PINCH/parvin complex: the kinase is dead, long live the pseudokinase!
journal, December 2009

Emerging roles of pseudokinases
journal, September 2006

A Modular Polycistronic Expression System for Overexpressing Protein Complexes in Escherichia coli
journal, February 2001

The regulation of integrin-linked kinase in human platelets: evidence for involvement in the regulation of integrin alpha2beta1
journal, August 2004

Spire and Cordon-bleu: multifunctional regulators of actin dynamics
journal, October 2008

The WH2 Domain and Actin Nucleation: Necessary but Insufficient
journal, June 2016

Enhanced CRISPR/Cas9-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA
journal, January 2017

Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties
journal, April 2007

TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts
journal, June 2009

Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin–ECM adhesion complex
journal, June 2008

Solution Structure of the Focal Adhesion Adaptor PINCH LIM1 Domain and Characterization of Its Interaction with the Integrin-linked Kinase Ankyrin Repeat Domain
journal, November 2000

The Pseudoactive Site of ILK Is Essential for Its Binding to α-Parvin and Localization to Focal Adhesions
journal, December 2009

Actin-bound structures of Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 and the implications for filament assembly
journal, November 2005

The Xplor-NIH NMR molecular structure determination package
journal, January 2003

The structural basis of integrin-linked kinase-PINCH interactions
journal, December 2008