Title: Enhancing Phenotyping and Molecular Analysis of Plant Root System Using Ultrasound Aeroponic Technology

Abstract

Abstract Several plant growth systems are available to enhance the observation of the root system (e.g., hydroponic and aeroponic plant growth systems, use of transparent soils, etc.). This article describes the use of the ultrasound aeroponic system (USAS) to treat and to enhance access to the root systems of various model plant and crop species (e.g., Arabidopsis thaliana , Medicago truncatula , soybean, etc.). This system is also compatible with short‐term (hr) and long‐term (days/weeks) biotic and abiotic treatments of plants. Upon treatment, the ease of access to the plant root system facilitates phenotyping (e.g., analysis of root architecture, establishment of root light spectrum using remote sensing technology), microscopic, molecular, and biochemical experiments. In addition, to facilitate functional genomic studies, we combined the use of the USAS with the hairy root transformation system to grow and observe transgenic roots on composite legume plants. © 2018 by John Wiley & Sons, Inc.

Authors:

Pingault, Lise ^[1]; Zogli, Prince ^[1]; Brooks, Jennifer ^[2]; Libault, Marc ^[1]

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+ Show Author Affiliations

1. Center for Plant Science Innovation, Department of Agronomy and Horticulture, Beadle Center, University of Nebraska‐Lincoln Lincoln Nebraska
2. Department of Microbiology and Plant Biology, George Lynn Cross Hall, University of Oklahoma, Norman Oklahoma

Publication Date:

Wed Oct 31 00:00:00 EDT 2018

Sponsoring Org.:

USDOE

OSTI Identifier:

1480147

Grant/Contract Number:  

DE‐SC0012629

Resource Type:

Publisher's Accepted Manuscript

Journal Name:

Current Protocols in Plant Biology

Additional Journal Information:

Journal Name: Current Protocols in Plant Biology Journal Volume: 3 Journal Issue: 4; Journal ID: ISSN 2379-8068

Publisher:

Wiley Blackwell (John Wiley & Sons)

Country of Publication:

United States

Language:

English