Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms
Abstract
ABSTRACT To better understand the impacts of Corexit 9500 on the structure and activity levels of hydrocarbon-degrading microbial communities, we analyzed next-generation 16S rRNA gene sequencing libraries of hydrocarbon enrichments grown at 5 and 25°C using both DNA and RNA extracts as the sequencing templates. Oil biodegradation patterns in both 5 and 25°C enrichments were consistent with those reported in the literature (i.e., aliphatics were degraded faster than aromatics). Slight increases in biodegradation were observed in the presence of Corexit at both temperatures. Differences in community structure were observed between treatment conditions in the DNA-based libraries. The 25°C consortia were dominated byVibrio,Idiomarina,Marinobacter,Alcanivorax, andThalassospiraspecies, while the 5°C consortia were dominated by several species of the generaFlavobacterium,Alcanivorax, andOleispira. Most of these genera have been linked to hydrocarbon degradation and have been observed after oil spills.ColwelliaandCycloclasticus, known aromatic degraders, were also found in these enrichments. The addition of Corexit did not have an effect on the active bacterial community structure of the 5°C consortia, while at 25°C, a decrease in the relative abundance ofMarinobacterwas observed. At 25°C,Thalassospira,Marinobacter, andIdiomarinawere present at higher relative abundances in the RNA than DNA libraries, suggesting that they were active in degradation. Similarly,Oleispirawas greatly stimulated by the addition ofmore »
- Authors:
-
- Michigan Technological Univ., Houghton, MI (United States)
- Univ. of Cincinnati, OH (United States)
- Pegasus, Inc., Cincinnati, OH (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- US Environmental Protection Agency (EPA), Cincinnati, OH (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1474527
- Alternate Identifier(s):
- OSTI ID: 1479312
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: 10; Journal ID: ISSN 0099-2240
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Techtmann, Stephen M., Zhuang, Mobing, Campo, Pablo, Holder, Edith, Elk, Michael, Hazen, Terry C., Conmy, Robyn, and Santo Domingo, Jorge W. Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms. United States: N. p., 2017.
Web. doi:10.1128/AEM.03462-16.
Techtmann, Stephen M., Zhuang, Mobing, Campo, Pablo, Holder, Edith, Elk, Michael, Hazen, Terry C., Conmy, Robyn, & Santo Domingo, Jorge W. Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms. United States. https://doi.org/10.1128/AEM.03462-16
Techtmann, Stephen M., Zhuang, Mobing, Campo, Pablo, Holder, Edith, Elk, Michael, Hazen, Terry C., Conmy, Robyn, and Santo Domingo, Jorge W. Fri .
"Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms". United States. https://doi.org/10.1128/AEM.03462-16. https://www.osti.gov/servlets/purl/1474527.
@article{osti_1474527,
title = {Corexit 9500 Enhances Oil Biodegradation and Changes Active Bacterial Community Structure of Oil-Enriched Microcosms},
author = {Techtmann, Stephen M. and Zhuang, Mobing and Campo, Pablo and Holder, Edith and Elk, Michael and Hazen, Terry C. and Conmy, Robyn and Santo Domingo, Jorge W.},
abstractNote = {ABSTRACT To better understand the impacts of Corexit 9500 on the structure and activity levels of hydrocarbon-degrading microbial communities, we analyzed next-generation 16S rRNA gene sequencing libraries of hydrocarbon enrichments grown at 5 and 25°C using both DNA and RNA extracts as the sequencing templates. Oil biodegradation patterns in both 5 and 25°C enrichments were consistent with those reported in the literature (i.e., aliphatics were degraded faster than aromatics). Slight increases in biodegradation were observed in the presence of Corexit at both temperatures. Differences in community structure were observed between treatment conditions in the DNA-based libraries. The 25°C consortia were dominated byVibrio,Idiomarina,Marinobacter,Alcanivorax, andThalassospiraspecies, while the 5°C consortia were dominated by several species of the generaFlavobacterium,Alcanivorax, andOleispira. Most of these genera have been linked to hydrocarbon degradation and have been observed after oil spills.ColwelliaandCycloclasticus, known aromatic degraders, were also found in these enrichments. The addition of Corexit did not have an effect on the active bacterial community structure of the 5°C consortia, while at 25°C, a decrease in the relative abundance ofMarinobacterwas observed. At 25°C,Thalassospira,Marinobacter, andIdiomarinawere present at higher relative abundances in the RNA than DNA libraries, suggesting that they were active in degradation. Similarly,Oleispirawas greatly stimulated by the addition of oil at 5°C. IMPORTANCEWhile dispersants such as Corexit 9500 can be used to treat oil spills, there is still debate on the effectiveness on enhancing oil biodegradation and its potential toxic effect on oil-degrading microbial communities. The results of this study provide some insights on the microbial dynamics of hydrocarbon-degrading bacterial populations in the presence of Corexit 9500. Operational taxonomic unit (OTU) analyses indicated that several OTUs were inhibited by the addition of Corexit. Conversely, a number of OTUs were stimulated by the addition of the dispersant, many of which were identified as known hydrocarbon-degrading bacteria. The results highlight the value of using RNA-based methods to further understand the impact of dispersant on the overall activity of different hydrocarbon-degrading bacterial groups.},
doi = {10.1128/AEM.03462-16},
journal = {Applied and Environmental Microbiology},
number = 10,
volume = 83,
place = {United States},
year = {Fri Mar 10 00:00:00 EST 2017},
month = {Fri Mar 10 00:00:00 EST 2017}
}
Web of Science
Works referenced in this record:
Effect of Initial Oil Concentration and Dispersant on Crude Oil Biodegradation in Contaminated Seawater
journal, March 2010
- Zahed, Mohammad Ali; Aziz, Hamidi Abdul; Isa, Mohamed Hasnain
- Bulletin of Environmental Contamination and Toxicology, Vol. 84, Issue 4
Marine microorganisms make a meal of oil
journal, March 2006
- Head, Ian M.; Jones, D. Martin; Röling, Wilfred F. M.
- Nature Reviews Microbiology, Vol. 4, Issue 3
UCHIME improves sensitivity and speed of chimera detection
journal, June 2011
- Edgar, Robert C.; Haas, Brian J.; Clemente, Jose C.
- Bioinformatics, Vol. 27, Issue 16
Effect of dispersants on the biodegradation of South Louisiana crude oil at 5 and 25 °C
journal, February 2016
- Zhuang, Mobing; Abulikemu, Gulizhaer; Campo, Pablo
- Chemosphere, Vol. 144
Metagenome, metatranscriptome and single-cell sequencing reveal microbial response to Deepwater Horizon oil spill
journal, June 2012
- Mason, Olivia U.; Hazen, Terry C.; Borglin, Sharon
- The ISME Journal, Vol. 6, Issue 9
Oil Biodegradation and Bioremediation: A Tale of the Two Worst Spills in U.S. History
journal, August 2011
- Atlas, Ronald M.; Hazen, Terry C.
- Environmental Science & Technology, Vol. 45, Issue 16, p. 6709-6715
PyNAST: a flexible tool for aligning sequences to a template alignment
journal, November 2009
- Caporaso, J. G.; Bittinger, K.; Bushman, F. D.
- Bioinformatics, Vol. 26, Issue 2
An Ordination of the Upland Forest Communities of Southern Wisconsin
journal, February 1957
- Bray, J. Roger; Curtis, J. T.
- Ecological Monographs, Vol. 27, Issue 4
Estimation of hydrocarbon biodegradation rates in marine environments: A critical review of the Q10 approach
journal, August 2013
- Bagi, Andrea; Pampanin, Daniela M.; Brakstad, Odd Gunnar
- Marine Environmental Research, Vol. 89
Hydrocarbon-Degrading Bacteria and the Bacterial Community Response in Gulf of Mexico Beach Sands Impacted by the Deepwater Horizon Oil Spill
journal, September 2011
- Kostka, Joel E.; Prakash, Om; Overholt, Will A.
- Applied and Environmental Microbiology, Vol. 77, Issue 22
Assessment of polyaromatic hydrocarbon degradation by potentially pathogenic environmental Vibrio parahaemolyticus isolates from coastal Louisiana, USA
journal, January 2012
- Smith, Conor B.; Johnson, Crystal N.; King, Gary M.
- Marine Pollution Bulletin, Vol. 64, Issue 1
What Genomic Sequence Information Has Revealed About Vibrio Ecology in the Ocean—A Review
journal, September 2009
- Grimes, Darrell Jay; Johnson, Crystal N.; Dillon, Kevin S.
- Microbial Ecology, Vol. 58, Issue 3
Large-scale cold water dispersant effectiveness experiments with Alaskan crude oils and Corexit 9500 and 9527 dispersants
journal, January 2009
- Belore, Randy C.; Trudel, Ken; Mullin, Joseph V.
- Marine Pollution Bulletin, Vol. 58, Issue 1
A new method for non-parametric multivariate analysis of variance: NON-PARAMETRIC MANOVA FOR ECOLOGY
journal, February 2001
- Anderson, Marti J.
- Austral Ecology, Vol. 26, Issue 1
Chemical dispersants can suppress the activity of natural oil-degrading microorganisms
journal, November 2015
- Kleindienst, Sara; Seidel, Michael; Ziervogel, Kai
- Proceedings of the National Academy of Sciences, Vol. 112, Issue 48
Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007
- Wang, Q.; Garrity, G. M.; Tiedje, J. M.
- Applied and Environmental Microbiology, Vol. 73, Issue 16
GP2 medium is an artificial seawater for culture or maintenance of marine organisms
journal, January 1984
- Spotte, Stephen; Adams, Gary; Bubucis, Patricia M.
- Zoo Biology, Vol. 3, Issue 3
Biodegradation of dispersed Macondo oil in seawater at low temperature and different oil droplet sizes
journal, April 2015
- Brakstad, Odd G.; Nordtug, Trond; Throne-Holst, Mimmi
- Marine Pollution Bulletin, Vol. 93, Issue 1-2
Long-Term Persistence of Dispersants following the Deepwater Horizon Oil Spill
journal, June 2014
- White, Helen K.; Lyons, Shelby L.; Harrison, Sarah J.
- Environmental Science & Technology Letters, Vol. 1, Issue 7
Use of hopane as a conservative biomarker for monitoring the bioremediation effectiveness of crude oil contaminating a sandy beach
journal, February 1997
- Venosa, A. D.; Suidan, M. T.; King, D.
- Journal of Industrial Microbiology and Biotechnology, Vol. 18, Issue 2-3
Long-term persistence of oil from the Exxon Valdez spill in two-layer beaches
journal, January 2010
- Li, Hailong; Boufadel, Michel C.
- Nature Geoscience, Vol. 3, Issue 2
Natural gas and temperature structured a microbial community response to the Deepwater Horizon oil spill
journal, October 2011
- Redmond, M. C.; Valentine, D. L.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 50
Search and clustering orders of magnitude faster than BLAST
journal, August 2010
- Edgar, Robert C.
- Bioinformatics, Vol. 26, Issue 19, p. 2460-2461
Impact of Heavy Metals on Transcriptional and Physiological Activity of Nitrifying Bacteria
journal, November 2015
- Kapoor, Vikram; Li, Xuan; Elk, Michael
- Environmental Science & Technology, Vol. 49, Issue 22
Effects of COREXIT® EC9500A on bacteria from a beach oiled by the Deepwater Horizon spill
journal, March 2011
- Hamdan, Lj; Fulmer, Pa
- Aquatic Microbial Ecology, Vol. 63, Issue 2
Microbial Response to the MC-252 Oil and Corexit 9500 in the Gulf of Mexico
journal, January 2012
- Chakraborty, Romy; Borglin, Sharon E.; Dubinsky, Eric A.
- Frontiers in Microbiology, Vol. 3
Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a New, Extremely Halotolerant, Hydrocarbon-Degrading Marine Bacterium
journal, October 1992
- Gauthier, M. J.; Lafay, B.; Christen, R.
- International Journal of Systematic Bacteriology, Vol. 42, Issue 4
Biodegradability of Corexit 9500 and Dispersed South Louisiana Crude Oil at 5 and 25 °C
journal, February 2013
- Campo, Pablo; Venosa, Albert D.; Suidan, Makram T.
- Environmental Science & Technology, Vol. 47, Issue 4
The primary biodegradation of dispersed crude oil in the sea
journal, January 2013
- Prince, Roger C.; McFarlin, Kelly M.; Butler, Josh D.
- Chemosphere, Vol. 90, Issue 2
Responses of Microbial Communities in Arctic Sea Ice After Contamination by Crude Petroleum Oil
journal, September 2007
- Brakstad, Odd Gunnar; Nonstad, Ingunn; Faksness, Liv-Guri
- Microbial Ecology, Vol. 55, Issue 3
Assessment of the Deepwater Horizon oil spill impact on Gulf coast microbial communities
journal, April 2014
- Lamendella, Regina; Strutt, Steven; Borglin, Sharon
- Frontiers in Microbiology, Vol. 5
Natural seepage of crude oil into the marine environment
journal, December 2003
- Kvenvolden, K. A.; Cooper, C. K.
- Geo-Marine Letters, Vol. 23, Issue 3-4
Oleispira antarctica gen. nov., sp. nov., a novel hydrocarbonoclastic marine bacterium isolated from Antarctic coastal sea water
journal, May 2003
- Yakimov, M. M.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 53, Issue 3
Single-cell genomics reveals features of a Colwellia species that was dominant during the Deepwater Horizon oil spill
journal, July 2014
- Mason, Olivia U.; Han, James; Woyke, Tanja
- Frontiers in Microbiology, Vol. 5
Succession of Hydrocarbon-Degrading Bacteria in the Aftermath of the Deepwater Horizon Oil Spill in the Gulf of Mexico
journal, September 2013
- Dubinsky, Eric A.; Conrad, Mark E.; Chakraborty, Romy
- Environmental Science & Technology, Vol. 47, Issue 19
Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP
journal, June 2013
- Gutierrez, Tony; Singleton, David R.; Berry, David
- The ISME Journal, Vol. 7, Issue 11
QIIME allows analysis of high-throughput community sequencing data
journal, April 2010
- Caporaso, J. Gregory; Kuczynski, Justin; Stombaugh, Jesse
- Nature Methods, Vol. 7, Issue 5
The use of hydrocarbon analyses for environmental assessment and remediation
journal, January 1992
- Douglas, G. S.; McCarthy, K. J.; Dahlen, D. T.
- Journal of Soil Contamination, Vol. 1, Issue 3
Differential abundance analysis for microbial marker-gene surveys
journal, September 2013
- Paulson, Joseph N.; Stine, O. Colin; Bravo, Héctor Corrada
- Nature Methods, Vol. 10, Issue 12
Fate of Dispersants Associated with the Deepwater Horizon Oil Spill
journal, February 2011
- Kujawinski, Elizabeth B.; Kido Soule, Melissa C.; Valentine, David L.
- Environmental Science & Technology, Vol. 45, Issue 4, p. 1298-1306
High Numbers of Vibrio vulnificus in Tar Balls Collected from Oiled Areas of the North-Central Gulf of Mexico Following the 2010 BP Deepwater Horizon Oil Spill
journal, November 2011
- Tao, Zhen; Bullard, Stephen; Arias, Covadonga
- EcoHealth, Vol. 8, Issue 4
Biodegradability of dispersed crude oil at two different temperatures
journal, May 2007
- Venosa, A. D.; Holder, E. L.
- Marine Pollution Bulletin, Vol. 54, Issue 5
Biodegradation of Dispersed Oil in Arctic Seawater at -1°C
journal, January 2014
- McFarlin, Kelly M.; Prince, Roger C.; Perkins, Robert
- PLoS ONE, Vol. 9, Issue 1
Marine Oil Biodegradation
journal, February 2016
- Hazen, Terry C.; Prince, Roger C.; Mahmoudi, Nagissa
- Environmental Science & Technology, Vol. 50, Issue 5
Psychrophiles - Cold-Adapted Hydrocarbon-Degrading Microorganisms
book, January 2010
- Giudice, A. Lo; Bruni, V.; De Domenico, M.
- Handbook of Hydrocarbon and Lipid Microbiology
Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms
journal, March 2012
- Caporaso, J. Gregory; Lauber, Christian L.; Walters, William A.
- The ISME Journal, Vol. 6, Issue 8
Deep-sea bacteria enriched by oil and dispersant from the Deepwater Horizon spill: Enrichment of oil degraders from Gulf of Mexico
journal, May 2012
- Baelum, Jacob; Borglin, Sharon; Chakraborty, Romy
- Environmental Microbiology, Vol. 14, Issue 9
UniFrac: a New Phylogenetic Method for Comparing Microbial Communities
journal, December 2005
- Lozupone, C.; Knight, R.
- Applied and Environmental Microbiology, Vol. 71, Issue 12
A new method for non-parametric multivariate analysis of variance
journal, February 2001
- Anderson, Marti J.
- Austral Ecology, Vol. 26, Issue 1
Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake
journal, March 2020
- Liu, Yang; Ren, Ze; Qu, Xiaodong
- Scientific Reports, Vol. 10, Issue 1
A new method for non-parametric multivariate analysis of variance
journal, February 2001
- Anderson, Marti J.
- Austral Ecology, Vol. 26, Issue 1
Hydrocarbon-degrading bacteria enriched by the Deepwater Horizon oil spill identified by cultivation and DNA-SIP
text, January 2013
- David, Berry,; D., Aitken, Michael; Tony, Gutierrez,
- The University of North Carolina at Chapel Hill University Libraries
Chemical dispersants can suppress the activity of natural oil-degrading microorganisms
text, January 2015
- Sarah, Harrison,; Y., Malkin, Sairah; Thorsten, Dittmar,
- The University of North Carolina at Chapel Hill University Libraries
Works referencing / citing this record:
Pressure and temperature effects on deep-sea hydrocarbon-degrading microbial communities in subarctic sediments
journal, November 2018
- Perez Calderon, Luis J.; Gontikaki, Evangelia; Potts, Lloyd D.
- MicrobiologyOpen, Vol. 8, Issue 6
Biodegradation of dispersed oil in natural seawaters from Western Greenland and a Norwegian fjord
journal, July 2018
- Brakstad, Odd G.; Davies, Emlyn J.; Ribicic, Deni
- Polar Biology, Vol. 41, Issue 12
Spatial and temporal dynamics of a freshwater eukaryotic plankton community revealed via 18S rRNA gene metabarcoding
journal, April 2018
- Banerji, A.; Bagley, M.; Elk, M.
- Hydrobiologia, Vol. 818, Issue 1
Chemical and biological dispersants differently affect the bacterial communities of uncontaminated and oil-contaminated marine water
journal, October 2019
- Rattes de Almeida Couto, Camila; Catharine de Assis Leite, Deborah; Jurelevicius, Diogo
- Brazilian Journal of Microbiology, Vol. 51, Issue 2
Effect of spatial origin and hydrocarbon composition on bacterial consortia community structure and hydrocarbon biodegradation rates
journal, June 2018
- Potts, Lloyd D.; Perez Calderon, Luis J.; Gontikaki, Evangelia
- FEMS Microbiology Ecology, Vol. 94, Issue 9
Hydrocarbon-Degrading Microbial Communities Are Site Specific, and Their Activity Is Limited by Synergies in Temperature and Nutrient Availability in Surface Ocean Waters
journal, May 2019
- Sun, Xiaoxu; Kostka, Joel E.
- Applied and Environmental Microbiology, Vol. 85, Issue 15
Response of microbial community structure and metabolic profile to shifts of inlet VOCs in a gas-phase biofilter
journal, October 2018
- Lu, Lichao; Wang, Guangchun; Yeung, Marvin
- AMB Express, Vol. 8, Issue 1
Rapid Formation of Microbe-Oil Aggregates and Changes in Community Composition in Coastal Surface Water Following Exposure to Oil and the Dispersant Corexit
journal, April 2018
- Doyle, Shawn M.; Whitaker, Emily A.; De Pascuale, Veronica
- Frontiers in Microbiology, Vol. 9
Biodegradation of Crude Oil and Corexit 9500 in Arctic Seawater
journal, August 2018
- McFarlin, Kelly M.; Perkins, Matt J.; Field, Jennifer A.
- Frontiers in Microbiology, Vol. 9
Dispersant Enhances Hydrocarbon Degradation and Alters the Structure of Metabolically Active Microbial Communities in Shallow Seawater From the Northeastern Gulf of Mexico
journal, October 2019
- Sun, Xiaoxu; Chu, Lena; Mercando, Elisa
- Frontiers in Microbiology, Vol. 10