Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry

Lu, Yue; Goodson, Carrie; Blankenship, Robert E.; Gross, Michael L.

doi:10.1021/acs.jproteome.7b00897

Title: Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry

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Abstract

The reaction center (RC) from the phototrophic bacterium Blastochloris viridis was the first integral membrane protein complex to have its structure determined by X-ray crystallography and has been studied extensively since then. It is composed of four protein subunits, H, M, L, and C, as well as cofactors, including bacteriopheophytin (BPh), bacteriochlorophyll (BCh), menaquinone, ubiquinone, heme, carotenoid, and Fe. In this study, we utilized mass spectrometry-based proteomics to study this protein complex via bottom-up sequencing, intact protein mass analysis, and native MS ligand-binding analysis. Its primary structure shows a series of mutations, including an unusual alteration and extension on the C-terminus of the M-subunit. In terms of quaternary structure, proteins such as this containing many cofactors serve to test the ability to introduce native-state protein assemblies into the gas phase because the cofactors will not be retained if the quaternary structure is seriously perturbed. Furthermore, this specific RC, under native MS, exhibits a strong ability not only to bind the special pair but also to preserve the two peripheral BCh’s.

Authors:

^[1]; Goodson, Carrie ^[2]; Blankenship, Robert E. ^[3];

^[1]

Washington Univ., St. Louis, MO (United States). Dept. of Chemistry, and Photosynthetic Antenna Research Center
Washington Univ., St. Louis, MO (United States). Photosynthetic Antenna Research Center, and Dept. of Biology
Washington Univ., St. Louis, MO (United States). Dept. of Chemistry, Photosynthetic Antenna Research Center, and Dept. of Biology

Publication Date:: 2018-02-21

Research Org.:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1470583

Grant/Contract Number:: SC0001035

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Proteome Research

Additional Journal Information:: Journal Volume: 17; Journal Issue: 4; Related Information: PARC partners with Washington University in St. Louis (lead); University of California, Riverside; University of Glasgow, UK; Los Alamos National Laboratory; University of New Mexico; New Mexico Corsortium; North Carolina State University; Northwestern University; Oak Ridge National Laboratory; University of Pennsylvania; Sandia National Laboratories; University of Sheffield, UK; Journal ID: ISSN 1535-3893

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; solar (fuels); photosynthesis (natural and artificial); biofuels (including algae and biomass); bio-inspired; charge transport; membrane; synthesis (novel materials); synthesis (self-assembly)

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                    Lu, Yue, Goodson, Carrie, Blankenship, Robert E., and Gross, Michael L. Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry.  United States: N. p., 2018. 
Web.  doi:10.1021/acs.jproteome.7b00897.

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                    Lu, Yue, Goodson, Carrie, Blankenship, Robert E., & Gross, Michael L. Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry.  United States.  https://doi.org/10.1021/acs.jproteome.7b00897

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                    Lu, Yue, Goodson, Carrie, Blankenship, Robert E., and Gross, Michael L. Wed .  
"Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry".  United States.  https://doi.org/10.1021/acs.jproteome.7b00897.  https://www.osti.gov/servlets/purl/1470583.

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@article{osti_1470583,

  title        = {Primary and Higher Order Structure of the Reaction Center from the Purple Phototrophic Bacterium Blastochloris viridis: A Test for Native Mass Spectrometry},

  author       = {Lu, Yue and Goodson, Carrie and Blankenship, Robert E. and Gross, Michael L.},

  abstractNote = {The reaction center (RC) from the phototrophic bacterium Blastochloris viridis was the first integral membrane protein complex to have its structure determined by X-ray crystallography and has been studied extensively since then. It is composed of four protein subunits, H, M, L, and C, as well as cofactors, including bacteriopheophytin (BPh), bacteriochlorophyll (BCh), menaquinone, ubiquinone, heme, carotenoid, and Fe. In this study, we utilized mass spectrometry-based proteomics to study this protein complex via bottom-up sequencing, intact protein mass analysis, and native MS ligand-binding analysis. Its primary structure shows a series of mutations, including an unusual alteration and extension on the C-terminus of the M-subunit. In terms of quaternary structure, proteins such as this containing many cofactors serve to test the ability to introduce native-state protein assemblies into the gas phase because the cofactors will not be retained if the quaternary structure is seriously perturbed. Furthermore, this specific RC, under native MS, exhibits a strong ability not only to bind the special pair but also to preserve the two peripheral BCh’s.},

  doi          = {10.1021/acs.jproteome.7b00897},

  journal      = {Journal of Proteome Research},

  number       = 4,

  volume       = 17,

  place        = {United States},

  year         = {2018},

  month        = {2}

}

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