High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner
Abstract
The Frontier Microfocus Macromolecular Crystallography (FMX) beamline at the National Synchrotron Light Source II with its 1 µm beam size and photon flux of 3 × 1012photons s−1at a photon energy of 12.66 keV has reached unprecedented dose rates for a structural biology beamline. The high dose rate presents a great advantage for serial microcrystallography in cutting measurement time from hours to minutes. To provide the instrumentation basis for such measurements at the full flux of the FMX beamline, a high-speed, high-precision goniometer based on a uniqueXYZpiezo positioner has been designed and constructed. The piezo-based goniometer is able to achieve sub-100 nm raster-scanning precision at over 10 grid-linepairs s−1frequency for fly scans of a 200 µm-wide raster. The performance of the scanner in both laboratory and serial crystallography measurements up to the maximum frame rate of 750 Hz of the Eiger 16M's 4M region-of-interest mode has been verified in this work. This unprecedented experimental speed significantly reduces serial-crystallography data collection time at synchrotrons, allowing utilization of the full brightness of the emerging synchrotron radiation facilities.
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Laboratory Directed Research and Development (LDRD) Program; National Institutes of Health (NIH)
- OSTI Identifier:
- 1466078
- Alternate Identifier(s):
- OSTI ID: 1476286
- Report Number(s):
- BNL-209117-2018-JAAM
Journal ID: ISSN 1600-5775; JSYRES; PII: S1600577518010354
- Grant/Contract Number:
- SC0012704; P41GM111244
- Resource Type:
- Published Article
- Journal Name:
- Journal of Synchrotron Radiation (Online)
- Additional Journal Information:
- Journal Name: Journal of Synchrotron Radiation (Online) Journal Volume: 25 Journal Issue: 5; Journal ID: ISSN 1600-5775
- Publisher:
- International Union of Crystallography
- Country of Publication:
- Denmark
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 43 PARTICLE ACCELERATORS; macromolecular crystallography; nano-positioning; serial crystallography; raster-scanning; high-precision goniometer
Citation Formats
Gao, Yuan, Xu, Weihe, Shi, Wuxian, Soares, Alexei, Jakoncic, Jean, Myers, Stuart, Martins, Bruno, Skinner, John, Liu, Qun, Bernstein, Herbert, McSweeney, Sean, Nazaretski, Evgeny, and Fuchs, Martin R. High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner. Denmark: N. p., 2018.
Web. doi:10.1107/S1600577518010354.
Gao, Yuan, Xu, Weihe, Shi, Wuxian, Soares, Alexei, Jakoncic, Jean, Myers, Stuart, Martins, Bruno, Skinner, John, Liu, Qun, Bernstein, Herbert, McSweeney, Sean, Nazaretski, Evgeny, & Fuchs, Martin R. High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner. Denmark. https://doi.org/10.1107/S1600577518010354
Gao, Yuan, Xu, Weihe, Shi, Wuxian, Soares, Alexei, Jakoncic, Jean, Myers, Stuart, Martins, Bruno, Skinner, John, Liu, Qun, Bernstein, Herbert, McSweeney, Sean, Nazaretski, Evgeny, and Fuchs, Martin R. Thu .
"High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner". Denmark. https://doi.org/10.1107/S1600577518010354.
@article{osti_1466078,
title = {High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner},
author = {Gao, Yuan and Xu, Weihe and Shi, Wuxian and Soares, Alexei and Jakoncic, Jean and Myers, Stuart and Martins, Bruno and Skinner, John and Liu, Qun and Bernstein, Herbert and McSweeney, Sean and Nazaretski, Evgeny and Fuchs, Martin R.},
abstractNote = {The Frontier Microfocus Macromolecular Crystallography (FMX) beamline at the National Synchrotron Light Source II with its 1 µm beam size and photon flux of 3 × 1012photons s−1at a photon energy of 12.66 keV has reached unprecedented dose rates for a structural biology beamline. The high dose rate presents a great advantage for serial microcrystallography in cutting measurement time from hours to minutes. To provide the instrumentation basis for such measurements at the full flux of the FMX beamline, a high-speed, high-precision goniometer based on a uniqueXYZpiezo positioner has been designed and constructed. The piezo-based goniometer is able to achieve sub-100 nm raster-scanning precision at over 10 grid-linepairs s−1frequency for fly scans of a 200 µm-wide raster. The performance of the scanner in both laboratory and serial crystallography measurements up to the maximum frame rate of 750 Hz of the Eiger 16M's 4M region-of-interest mode has been verified in this work. This unprecedented experimental speed significantly reduces serial-crystallography data collection time at synchrotrons, allowing utilization of the full brightness of the emerging synchrotron radiation facilities.},
doi = {10.1107/S1600577518010354},
journal = {Journal of Synchrotron Radiation (Online)},
number = 5,
volume = 25,
place = {Denmark},
year = {Thu Aug 23 00:00:00 EDT 2018},
month = {Thu Aug 23 00:00:00 EDT 2018}
}
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https://doi.org/10.1107/S1600577518010354
https://doi.org/10.1107/S1600577518010354
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Cited by: 16 works
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