Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae)
Abstract
• Premise of the study: Are there dimensions of symbiotic root interactions that are overlooked because plant mineral nutrition is the foundation and, perhaps too often, the sole explanation through which we view these relationships? In this paper we investigate how the root nodule symbiosis in selenium (Se) hyperaccumulator and nonaccumulator Astragalus species influences plant selenium (Se) accumulation. • Methods: In greenhouse studies, Se was added to nodulated and nonnodulated hyperaccumulator and nonaccumulator Astragalus plants, followed by investigation of nitrogen (N)–Se relationships. Selenium speciation was also investigated, using x‐ray microprobe analysis and liquid chromatography–mass spectrometry (LC‐MS). • Key results: Nodulation enhanced biomass production and Se to S ratio in both hyperaccumulator and nonaccumulator plants. The hyperaccumulator contained more Se when nodulated, while the nonaccumulator contained less S when nodulated. Shoot [Se] was positively correlated with shoot N in Se‐hyperaccumulator species, but not in nonhyperaccumulator species. The x‐ray microprobe analysis showed that hyperaccumulators contain significantly higher amounts of organic Se than nonhyperaccumulators. LC‐MS of A. bisulcatus leaves revealed that nodulated plants contained more γ‐glutamyl‐methylselenocysteine (γ‐Glu‐MeSeCys) than nonnodulated plants, while MeSeCys levels were similar. • Conclusions: Root nodule mutualism positively affects Se hyperaccumulation in Astragalus . The microbial N supply particularly appearsmore »
- Authors:
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1464385
- Grant/Contract Number:
- DE‐AC02‐05CH11231
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- American Journal of Botany
- Additional Journal Information:
- Journal Name: American Journal of Botany Journal Volume: 101 Journal Issue: 11; Journal ID: ISSN 0002-9122
- Publisher:
- Wiley Blackwell (John Wiley & Sons)
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Alford, Élan R., Lindblom, Stormy D., Pittarello, Marco, Freeman, John L., Fakra, Sirine C., Marcus, Matthew A., Broeckling, Corey, Pilon‐Smits, Elizabeth A. H., and Paschke, Mark W. Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae). United States: N. p., 2014.
Web. doi:10.3732/ajb.1400223.
Alford, Élan R., Lindblom, Stormy D., Pittarello, Marco, Freeman, John L., Fakra, Sirine C., Marcus, Matthew A., Broeckling, Corey, Pilon‐Smits, Elizabeth A. H., & Paschke, Mark W. Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae). United States. https://doi.org/10.3732/ajb.1400223
Alford, Élan R., Lindblom, Stormy D., Pittarello, Marco, Freeman, John L., Fakra, Sirine C., Marcus, Matthew A., Broeckling, Corey, Pilon‐Smits, Elizabeth A. H., and Paschke, Mark W. Sat .
"Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae)". United States. https://doi.org/10.3732/ajb.1400223.
@article{osti_1464385,
title = {Roles of rhizobial symbionts in selenium hyperaccumulation in Astragalus (Fabaceae)},
author = {Alford, Élan R. and Lindblom, Stormy D. and Pittarello, Marco and Freeman, John L. and Fakra, Sirine C. and Marcus, Matthew A. and Broeckling, Corey and Pilon‐Smits, Elizabeth A. H. and Paschke, Mark W.},
abstractNote = {• Premise of the study: Are there dimensions of symbiotic root interactions that are overlooked because plant mineral nutrition is the foundation and, perhaps too often, the sole explanation through which we view these relationships? In this paper we investigate how the root nodule symbiosis in selenium (Se) hyperaccumulator and nonaccumulator Astragalus species influences plant selenium (Se) accumulation. • Methods: In greenhouse studies, Se was added to nodulated and nonnodulated hyperaccumulator and nonaccumulator Astragalus plants, followed by investigation of nitrogen (N)–Se relationships. Selenium speciation was also investigated, using x‐ray microprobe analysis and liquid chromatography–mass spectrometry (LC‐MS). • Key results: Nodulation enhanced biomass production and Se to S ratio in both hyperaccumulator and nonaccumulator plants. The hyperaccumulator contained more Se when nodulated, while the nonaccumulator contained less S when nodulated. Shoot [Se] was positively correlated with shoot N in Se‐hyperaccumulator species, but not in nonhyperaccumulator species. The x‐ray microprobe analysis showed that hyperaccumulators contain significantly higher amounts of organic Se than nonhyperaccumulators. LC‐MS of A. bisulcatus leaves revealed that nodulated plants contained more γ‐glutamyl‐methylselenocysteine (γ‐Glu‐MeSeCys) than nonnodulated plants, while MeSeCys levels were similar. • Conclusions: Root nodule mutualism positively affects Se hyperaccumulation in Astragalus . The microbial N supply particularly appears to contribute glutamate for the formation of γ‐Glu‐MeSeCys. Our results provide insight into the significance of symbiotic interactions in plant adaptation to edaphic conditions. Specifically, our findings illustrate that the importance of these relationships are not limited to alleviating macronutrient deficiencies.},
doi = {10.3732/ajb.1400223},
journal = {American Journal of Botany},
number = 11,
volume = 101,
place = {United States},
year = {Sat Nov 01 00:00:00 EDT 2014},
month = {Sat Nov 01 00:00:00 EDT 2014}
}
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