Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer

Abstract

Flowers of the hop plant provide both bitterness and "hoppy" flavor to beer. Hops are, however, both a water and energy intensive crop and vary considerably in essential oil content, making it challenging to achieve a consistent hoppy taste in beer. Here, we report that brewer's yeast can be engineered to biosynthesize aromatic monoterpene molecules that impart hoppy flavor to beer by incorporating recombinant DNA derived from yeast, mint, and basil. Whereas metabolic engineering of biosynthetic pathways is commonly enlisted to maximize product titers, tuning expression of pathway enzymes to affect target production levels of multiple commercially important metabolites without major collateral metabolic changes represents a unique challenge. By applying state-of-the-art engineering techniques and a framework to guide iterative improvement, strains are generated with target performance characteristics. Finally, beers produced using these strains are perceived as hoppier than traditionally hopped beers by a sensory panel in a double-blind tasting.

Authors:
 [1];  [2];  [3];  [4];  [1];  [5];  [6];  [7]; ORCiD logo [6];  [5];  [8]; ORCiD logo [4]; ORCiD logo [9]
+ Show Author Affiliations
  1. Univ. of California, Berkeley, CA (United States). California Inst. of Quantitative Biosciences (QB3); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  3. Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
  4. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; DOE Agile BioFoundry, Emeryville, CA (United States)
  5. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
  6. Univ. of California, Davis, CA (United States). Dept. of Food Science and Technology
  7. Lagunitas Brewing Company, Petaluma, CA (United States)
  8. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Environmental Genomics and Systems Biology Division
  9. Univ. of California, Berkeley, CA (United States). California Inst. of Quantitative Biosciences (QB3); Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering; Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering; Technical Univ. of Denmark, Hellerup (Denmark). Novo Nordisk Foundation Center for Sustainability
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1457017
Grant/Contract Number:  
AC02-05CH11231; 1722376
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Denby, Charles M., Li, Rachel A., Vu, Van T., Costello, Zak, Lin, Weiyin, Chan, Leanne Jade G., Williams, Joseph, Donaldson, Bryan, Bamforth, Charles W., Petzold, Christopher J., Scheller, Henrik V., Martin, Hector Garcia, and Keasling, Jay D. Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer. United States: N. p., 2018. Web. doi:10.1038/s41467-018-03293-x.
Copy to clipboard
Denby, Charles M., Li, Rachel A., Vu, Van T., Costello, Zak, Lin, Weiyin, Chan, Leanne Jade G., Williams, Joseph, Donaldson, Bryan, Bamforth, Charles W., Petzold, Christopher J., Scheller, Henrik V., Martin, Hector Garcia, & Keasling, Jay D. Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer. United States. https://doi.org/10.1038/s41467-018-03293-x
Copy to clipboard
Denby, Charles M., Li, Rachel A., Vu, Van T., Costello, Zak, Lin, Weiyin, Chan, Leanne Jade G., Williams, Joseph, Donaldson, Bryan, Bamforth, Charles W., Petzold, Christopher J., Scheller, Henrik V., Martin, Hector Garcia, and Keasling, Jay D. Tue . "Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer". United States. https://doi.org/10.1038/s41467-018-03293-x. https://www.osti.gov/servlets/purl/1457017.
Copy to clipboard
@article{osti_1457017,
title = {Industrial brewing yeast engineered for the production of primary flavor determinants in hopped beer},
author = {Denby, Charles M. and Li, Rachel A. and Vu, Van T. and Costello, Zak and Lin, Weiyin and Chan, Leanne Jade G. and Williams, Joseph and Donaldson, Bryan and Bamforth, Charles W. and Petzold, Christopher J. and Scheller, Henrik V. and Martin, Hector Garcia and Keasling, Jay D.},
abstractNote = {Flowers of the hop plant provide both bitterness and "hoppy" flavor to beer. Hops are, however, both a water and energy intensive crop and vary considerably in essential oil content, making it challenging to achieve a consistent hoppy taste in beer. Here, we report that brewer's yeast can be engineered to biosynthesize aromatic monoterpene molecules that impart hoppy flavor to beer by incorporating recombinant DNA derived from yeast, mint, and basil. Whereas metabolic engineering of biosynthetic pathways is commonly enlisted to maximize product titers, tuning expression of pathway enzymes to affect target production levels of multiple commercially important metabolites without major collateral metabolic changes represents a unique challenge. By applying state-of-the-art engineering techniques and a framework to guide iterative improvement, strains are generated with target performance characteristics. Finally, beers produced using these strains are perceived as hoppier than traditionally hopped beers by a sensory panel in a double-blind tasting.},
doi = {10.1038/s41467-018-03293-x},
journal = {Nature Communications},
number = 1,
volume = 9,
place = {United States},
year = {Tue Mar 20 00:00:00 EDT 2018},
month = {Tue Mar 20 00:00:00 EDT 2018}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1038/s41467-018-03293-x
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 122 works
Citation information provided by
Web of Science

Figures / Tables:

Fig. 1 Fig. 1: Engineering brewer’s yeast to express monoterpene biosynthetic pathways thereby replacing flavor hop addition. a During the brewing process, S. cerevisiae converts wort—a barley extract solution rich in fermentable sugars—into ethanol and other by-products. Hops are added immediately before, during, or after fermentation to impart “hoppy” flavor. Engineered strainsmore » produce linalool and geraniol, primary flavor components of hoppy beer, thereby replacing hop additions. b Six full-length plant-derived linalool synthase genes, as well as PTS-truncated variants, were expressed on high-copy plasmids. Full-length genes and PTS-truncated genes predicted by either ChloroP (C) or the RR-heuristic method (RR) are indicated by colored lines. c Error bars correspond to mean ± standard deviation of three biological replicates. Asterisks indicate statistically significant increases in monoterpene production compared with the control strain (Y) as determined by a t-test using p-value <0.025. The LIS from the California wildflower Clarkia breweri has been shown to increase production of linalool when heterologously expressed in plants and in yeast. However, when C. breweri LIS was expressed, either with native codons (nCb) or “yeast-optimized” codons (Cb), linalool was not detected. The Mentha citrata LIS (Mc) truncated at the RR motif was identified as sufficiently active to allow for monoterpene production at levels characteristic of commercial beer and was chosen for integration into brewer’s yeast strains« less
All figures and tables (5 total)
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Standard Flow Liquid Chromatography for Shotgun Proteomics in Bioenergy Research
journal, April 2015

  • González Fernández-Niño, Susana M.; Smith-Moritz, A. Michelle; Chan, Leanne Jade G.
  • Frontiers in Bioengineering and Biotechnology, Vol. 3
  • DOI: 10.3389/fbioe.2015.00044

ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites
journal, January 1999

  • Emanuelsson, Olof; Nielsen, Henrik; Heijne, Gunnar Von
  • Protein Science, Vol. 8, Issue 5
  • DOI: 10.1110/ps.8.5.978

Molecular cloning and characterization of a new linalool synthase
journal, September 2002

  • Crowell, Anastasia L.; Williams, David C.; Davis, Edward M.
  • Archives of Biochemistry and Biophysics, Vol. 405, Issue 1
  • DOI: 10.1016/S0003-9861(02)00348-X

Floral hop aroma in beer
journal, November 1981

  • Peacock, Val E.; Deinzer, Max L.; Likens, Sam T.
  • Journal of Agricultural and Food Chemistry, Vol. 29, Issue 6
  • DOI: 10.1021/jf00108a041

Enhanced Levels of the Aroma and Flavor Compound S- Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits
journal, November 2001

  • Lewinsohn, Efraim; Schalechet, Fernond; Wilkinson, Jack
  • Plant Physiology, Vol. 127, Issue 3
  • DOI: 10.1104/pp.010293

j5 DNA Assembly Design Automation Software
journal, December 2011

  • Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
  • ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
  • DOI: 10.1021/sb2000116

mProphet: automated data processing and statistical validation for large-scale SRM experiments
journal, March 2011

  • Reiter, Lukas; Rinner, Oliver; Picotti, Paola
  • Nature Methods, Vol. 8, Issue 5
  • DOI: 10.1038/nmeth.1584

Large-Scale Selection and Breeding To Generate Industrial Yeasts with Superior Aroma Production
journal, September 2014

  • Steensels, Jan; Meersman, Esther; Snoek, Tim
  • Applied and Environmental Microbiology, Vol. 80, Issue 22
  • DOI: 10.1128/AEM.02235-14

Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
journal, February 2010

A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
journal, April 2015

  • Lee, Michael E.; DeLoache, William C.; Cervantes, Bernardo
  • ACS Synthetic Biology, Vol. 4, Issue 9
  • DOI: 10.1021/sb500366v

Terpene Biosynthesis in Glandular Trichomes of Hop
journal, September 2008

Regulation of HMG-CoA reductase in mammals and yeast
journal, October 2011

Production of the antimalarial drug precursor artemisinic acid in engineered yeast
journal, April 2006

  • Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario
  • Nature, Vol. 440, Issue 7086, p. 940-943
  • DOI: 10.1038/nature04640

Plant terpenoid synthases: Molecular biology and phylogenetic analysis
journal, April 1998

  • Bohlmann, J.; Meyer-Gauen, G.; Croteau, R.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 8
  • DOI: 10.1073/pnas.95.8.4126

Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast
journal, January 1998

  • Polakowski, T.; Stahl, U.; Lang, C.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 1
  • DOI: 10.1007/s002530051138

Use of GC−Olfactometry to Identify the Hop Aromatic Compounds in Beer
journal, August 2001

  • Lermusieau, Guillaume; Bulens, Marie; Collin, Sonia
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 8
  • DOI: 10.1021/jf0101509

Secreting Glandular Trichomes: More than Just Hairs
journal, July 1991

Varietal Dependence of hop Flavour Volatiles in Lager
journal, May 1989

Functional optimization of gene clusters by combinatorial design and assembly
journal, November 2014

  • Smanski, Michael J.; Bhatia, Swapnil; Zhao, Dehua
  • Nature Biotechnology, Vol. 32, Issue 12, p. 1241-1249
  • DOI: 10.1038/nbt.3063

DeviceEditor visual biological CAD canvas
journal, December 2012

  • Chen, Joanna; Densmore, Douglas; Ham, Timothy S.
  • Journal of Biological Engineering, Vol. 6, Issue 1
  • DOI: 10.1186/1754-1611-6-1

ggplot2: Elegant Graphics for Data Analysis by WICKHAM, H.
journal, June 2011

Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine
journal, March 2008

Modern Applied Statistics with S
book, August 2002

De novo production of six key grape aroma monoterpenes by a geraniol synthase-engineered S. cerevisiae wine strain
journal, September 2015

Design, implementation and practice of JBEI-ICE: an open source biological part registry platform and tools
journal, June 2012

  • Ham, T. S.; Dmytriv, Z.; Plahar, H.
  • Nucleic Acids Research, Vol. 40, Issue 18
  • DOI: 10.1093/nar/gks531

Reshaping Data with the reshape Package
journal, January 2007

Quantitation of ( R )- and ( S )-Linalool in Beer Using Solid Phase Microextraction (SPME) in Combination with a Stable Isotope Dilution Assay (SIDA)
journal, November 2003

  • Steinhaus, Martin; Fritsch, Helge T.; Schieberle, Peter
  • Journal of Agricultural and Food Chemistry, Vol. 51, Issue 24
  • DOI: 10.1021/jf0347057

Expression Levels of the Yeast Alcohol Acetyltransferase Genes ATF1, Lg-ATF1, and ATF2 Control the Formation of a Broad Range of Volatile Esters
journal, September 2003

  • Verstrepen, K. J.; Van Laere, S. D. M.; Vanderhaegen, B. M. P.
  • Applied and Environmental Microbiology, Vol. 69, Issue 9
  • DOI: 10.1128/AEM.69.9.5228-5237.2003

A One Pot, One Step, Precision Cloning Method with High Throughput Capability
journal, November 2008

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
journal, September 2010

Enzymatic assembly of DNA molecules up to several hundred kilobases
journal, April 2009

  • Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan
  • Nature Methods, Vol. 6, Issue 5, p. 343-345
  • DOI: 10.1038/nmeth.1318

Improving industrial yeast strains: exploiting natural and artificial diversity
journal, September 2014

  • Steensels, Jan; Snoek, Tim; Meersman, Esther
  • FEMS Microbiology Reviews, Vol. 38, Issue 5
  • DOI: 10.1111/1574-6976.12073

Engineering Monoterpene Production in Yeast Using a Synthetic Dominant Negative Geranyl Diphosphate Synthase
journal, January 2014

  • Ignea, Codruta; Pontini, Marianna; Maffei, Massimo E.
  • ACS Synthetic Biology, Vol. 3, Issue 5
  • DOI: 10.1021/sb400115e

mProphet: automated data processing and statistical validation for large-scale SRM experiments
text, January 2011

Varietal Dependence of hop Flavour Volatiles in Lager
journal, May 1989

Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast
journal, January 1998

  • Polakowski, T.; Stahl, U.; Lang, C.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 1
  • DOI: 10.1007/s002530051138

Engineering the Saccharomyces cerevisiae isoprenoid pathway for de novo production of aromatic monoterpenes in wine
journal, March 2008

Floral hop aroma in beer
journal, November 1981

  • Peacock, Val E.; Deinzer, Max L.; Likens, Sam T.
  • Journal of Agricultural and Food Chemistry, Vol. 29, Issue 6
  • DOI: 10.1021/jf00108a041

Use of GC−Olfactometry to Identify the Hop Aromatic Compounds in Beer
journal, August 2001

  • Lermusieau, Guillaume; Bulens, Marie; Collin, Sonia
  • Journal of Agricultural and Food Chemistry, Vol. 49, Issue 8
  • DOI: 10.1021/jf0101509

Quantitation of ( R )- and ( S )-Linalool in Beer Using Solid Phase Microextraction (SPME) in Combination with a Stable Isotope Dilution Assay (SIDA)
journal, November 2003

  • Steinhaus, Martin; Fritsch, Helge T.; Schieberle, Peter
  • Journal of Agricultural and Food Chemistry, Vol. 51, Issue 24
  • DOI: 10.1021/jf0347057

j5 DNA Assembly Design Automation Software
journal, December 2011

  • Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D.
  • ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21
  • DOI: 10.1021/sb2000116

Production of the antimalarial drug precursor artemisinic acid in engineered yeast
journal, April 2006

  • Ro, Dae-Kyun; Paradise, Eric M.; Ouellet, Mario
  • Nature, Vol. 440, Issue 7086, p. 940-943
  • DOI: 10.1038/nature04640

Functional optimization of gene clusters by combinatorial design and assembly
journal, November 2014

  • Smanski, Michael J.; Bhatia, Swapnil; Zhao, Dehua
  • Nature Biotechnology, Vol. 32, Issue 12, p. 1241-1249
  • DOI: 10.1038/nbt.3063

Machine learning discovery of missing links that mediate alternative branches to plant alkaloids
journal, March 2022

  • Vavricka, Christopher J.; Takahashi, Shunsuke; Watanabe, Naoki
  • Nature Communications, Vol. 13, Issue 1
  • DOI: 10.1038/s41467-022-28883-8

Estimating nitrogen and phosphorus concentrations in streams and rivers, within a machine learning framework
journal, May 2020

Effect of design geometry, exposure energy, cytophilic molecules, cell type and load in fabrication of single-cell arrays using micro-contact printing
journal, September 2020

Plant terpenoid synthases: Molecular biology and phylogenetic analysis
journal, April 1998

  • Bohlmann, J.; Meyer-Gauen, G.; Croteau, R.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 8
  • DOI: 10.1073/pnas.95.8.4126

Enhanced Levels of the Aroma and Flavor Compound S- Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits
journal, November 2001

  • Lewinsohn, Efraim; Schalechet, Fernond; Wilkinson, Jack
  • Plant Physiology, Vol. 127, Issue 3
  • DOI: 10.1104/pp.010293

Terpene Biosynthesis in Glandular Trichomes of Hop
journal, September 2008

ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites
journal, January 1999

  • Emanuelsson, Olof; Nielsen, Henrik; Heijne, Gunnar Von
  • Protein Science, Vol. 8, Issue 5
  • DOI: 10.1110/ps.8.5.978

Improving industrial yeast strains: exploiting natural and artificial diversity
journal, September 2014

  • Steensels, Jan; Snoek, Tim; Meersman, Esther
  • FEMS Microbiology Reviews, Vol. 38, Issue 5
  • DOI: 10.1111/1574-6976.12073

De novo production of six key grape aroma monoterpenes by a geraniol synthase-engineered S. cerevisiae wine strain
journal, September 2015

Standard Flow Liquid Chromatography for Shotgun Proteomics in Bioenergy Research
journal, April 2015

  • González Fernández-Niño, Susana M.; Smith-Moritz, A. Michelle; Chan, Leanne Jade G.
  • Frontiers in Bioengineering and Biotechnology, Vol. 3
  • DOI: 10.3389/fbioe.2015.00044

mProphet: automated data processing and statistical validation for large-scale SRM experiments
text, January 2011

    Sort by:
    [ × clear filter / sort ]

    Works referencing / citing this record:

    CRISPR/Cas9‐RNA interference system for combinatorial metabolic engineering of Saccharomyces cerevisiae
    journal, May 2019

    • Kildegaard, Kanchana Rueksomtawin; Tramontin, Larissa Ribeiro Ramos; Chekina, Ksenia
    • Yeast, Vol. 36, Issue 5
    • DOI: 10.1002/yea.3390

    The renaissance of yeasts as microbial factories in the modern age of biomanufacturing
    journal, September 2019

    Yeast bioprospecting versus synthetic biology—which is better for innovative beverage fermentation?
    journal, January 2020

    • Alperstein, Lucien; Gardner, Jennifer M.; Sundstrom, Joanna F.
    • Applied Microbiology and Biotechnology, Vol. 104, Issue 5
    • DOI: 10.1007/s00253-020-10364-x

    Progress in the development of methods used for the abatement of microbial contaminants in ethanol fermentations: a review
    journal, August 2019

    • Sekoai, Patrick T.; Mhlongo, Sizwe I.; Ezeokoli, Obinna T.
    • Reviews in Environmental Science and Bio/Technology, Vol. 18, Issue 4
    • DOI: 10.1007/s11157-019-09511-2

    Synthetic biology strategies for microbial biosynthesis of plant natural products
    journal, May 2019

    Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate
    journal, August 2019

    Rerouting of NADPH synthetic pathways for increased protopanaxadiol production in Saccharomyces cerevisiae
    journal, October 2018

    Advanced strategy for metabolite exploration in filamentous fungi
    journal, January 2020

    Genome editing to generate nonfoam-forming sake yeast strains
    journal, December 2018

    • Ohnuki, Shinsuke; Kashima, Mao; Yamada, Toshikazu
    • Bioscience, Biotechnology, and Biochemistry, Vol. 83, Issue 8
    • DOI: 10.1080/09168451.2019.1631146

    The molecular biology of fruity and floral aromas in beer and other alcoholic beverages
    journal, November 2018

    • Holt, Sylvester; Miks, Marta H.; de Carvalho, Bruna Trindade
    • FEMS Microbiology Reviews, Vol. 43, Issue 3
    • DOI: 10.1093/femsre/fuy041

    Lager-brewing yeasts in the era of modern genetics
    journal, September 2019

    • Gorter de Vries, Arthur R.; Pronk, Jack T.; Daran, Jean-Marc G.
    • FEMS Yeast Research, Vol. 19, Issue 7
    • DOI: 10.1093/femsyr/foz063

    Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering
    journal, November 2018

    • Taylor, George M.; Mordaka, Paweł M.; Heap, John T.
    • Nucleic Acids Research, Vol. 47, Issue 3
    • DOI: 10.1093/nar/gky1182

    High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA
    journal, June 2019

    • O’Kane, Patrick T.; Dudley, Quentin M.; McMillan, Aislinn K.
    • Science Advances, Vol. 5, Issue 6
    • DOI: 10.1126/sciadv.aaw9180

    Towards semi-synthetic microbial communities: enhancing soy sauce fermentation properties in B. subtilis co-cultures
    journal, June 2019

    Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products
    journal, December 2018

    • Shaver, Zachary M.; Bent, Stephanie S.; Bilby, Steven R.
    • BMC Research Notes, Vol. 11, Issue 1
    • DOI: 10.1186/s13104-018-3965-6

    Microbial Platform for Terpenoid Production: Escherichia coli and Yeast
    journal, October 2018

    Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview
    journal, December 2019

    Terpenoid Metabolic Engineering in Photosynthetic Microorganisms
    journal, October 2018

    • Vavitsas, Konstantinos; Fabris, Michele; Vickers, Claudia
    • Genes, Vol. 9, Issue 11
    • DOI: 10.3390/genes9110520

    The rise of bottom-up synthetic biology and cell-free biology
    journal, May 2019

    CRISPR/Cas9‐RNA interference system for combinatorial metabolic engineering of Saccharomyces cerevisiae
    journal, May 2019

    • Kildegaard, Kanchana Rueksomtawin; Tramontin, Larissa Ribeiro Ramos; Chekina, Ksenia
    • Yeast, Vol. 36, Issue 5
    • DOI: 10.1002/yea.3390

    Synthetic biology strategies for microbial biosynthesis of plant natural products
    journal, May 2019

    Orthogonal monoterpenoid biosynthesis in yeast constructed on an isomeric substrate
    journal, August 2019

    A machine learning Automated Recommendation Tool for synthetic biology
    journal, September 2020

    Rerouting of NADPH synthetic pathways for increased protopanaxadiol production in Saccharomyces cerevisiae
    journal, October 2018

    Lager-brewing yeasts in the era of modern genetics
    journal, September 2019

    • Gorter de Vries, Arthur R.; Pronk, Jack T.; Daran, Jean-Marc G.
    • FEMS Yeast Research, Vol. 19, Issue 7
    • DOI: 10.1093/femsyr/foz063

    High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA
    journal, June 2019

    • O’Kane, Patrick T.; Dudley, Quentin M.; McMillan, Aislinn K.
    • Science Advances, Vol. 5, Issue 6
    • DOI: 10.1126/sciadv.aaw9180

    Towards semi-synthetic microbial communities: enhancing soy sauce fermentation properties in B. subtilis co-cultures
    journal, June 2019

    Attempted use of PACE for riboswitch discovery generates three new translational theophylline riboswitch side products
    journal, December 2018

    • Shaver, Zachary M.; Bent, Stephanie S.; Bilby, Steven R.
    • BMC Research Notes, Vol. 11, Issue 1
    • DOI: 10.1186/s13104-018-3965-6

    Beverage and Food Fragrance Biotechnology, Novel Applications, Sensory and Sensor Techniques: An Overview
    journal, December 2019

    Applications of Yeast Synthetic Biology Geared towards the Production of Biopharmaceuticals
    journal, July 2018

    Terpenoid Metabolic Engineering in Photosynthetic Microorganisms
    journal, October 2018

    • Vavitsas, Konstantinos; Fabris, Michele; Vickers, Claudia
    • Genes, Vol. 9, Issue 11
    • DOI: 10.3390/genes9110520
      Sort by:
      [ × clear filter / sort ]

      Figures / Tables found in this record:

      Fig. 1 (p. 2)figure
      Fig. 2 (p. 3)figure
      Fig. 3 (p. 4)figure
      Fig. 4 (p. 5)figure
      Fig. 5 (p. 6)figure
        [ × clear filter / sort ]
        Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.

        Similar Records in DOE PAGES and OSTI.GOV collections: