Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange
Abstract
Utilization of high-resolution molecular tomography revealed that the soybean nodule is not metabolically uniform, with several distinctive patterns of metabolite distributions through the nodule’s anatomy. Specifically, using two- and three-dimensional matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging (MALDI-FTICR MSI) metabolites showed i) cortex localization, ii) a gradual increase toward the central infection zone, iii) uniform localization through whole nodule, iv) uniform localization throughout the infection zone, or v) asymmetric distribution within the infection zone. Some molecules belonging to the same or correlated metabolic pathways were found localized with different patterns, indicating localized differences in their synthesis/turnover. Special attention was given to asymmetrical distributed metabolites (e.g., S-adenosylmethionine; SAMe), where some small-scale patterns were detected in individual sections, and 3D reconstruction enabled resolution of whole nodule molecular distributions. These results hint at a complex, spatially differentiated metabolism occurring in soybean root nodules.
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1454346
- Alternate Identifier(s):
- OSTI ID: 1458774; OSTI ID: 1513206
- Report Number(s):
- PNNL-SA-127419
Journal ID: ISSN 1751-7362
- Grant/Contract Number:
- DOE-FOA-0001192; 1734145; AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Name: The ISME Journal Journal Volume: 12 Journal Issue: 9; Journal ID: ISSN 1751-7362
- Publisher:
- Oxford University Press
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Veličković, Dušan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Paša-Tolić, Ljiljana, Stacey, Gary, and Anderton, Christopher R. Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange. United Kingdom: N. p., 2018.
Web. doi:10.1038/s41396-018-0188-8.
Veličković, Dušan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Paša-Tolić, Ljiljana, Stacey, Gary, & Anderton, Christopher R. Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange. United Kingdom. https://doi.org/10.1038/s41396-018-0188-8
Veličković, Dušan, Agtuca, Beverly J., Stopka, Sylwia A., Vertes, Akos, Koppenaal, David W., Paša-Tolić, Ljiljana, Stacey, Gary, and Anderton, Christopher R. Wed .
"Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange". United Kingdom. https://doi.org/10.1038/s41396-018-0188-8.
@article{osti_1454346,
title = {Observed metabolic asymmetry within soybean root nodules reflects unexpected complexity in rhizobacteria-legume metabolite exchange},
author = {Veličković, Dušan and Agtuca, Beverly J. and Stopka, Sylwia A. and Vertes, Akos and Koppenaal, David W. and Paša-Tolić, Ljiljana and Stacey, Gary and Anderton, Christopher R.},
abstractNote = {Utilization of high-resolution molecular tomography revealed that the soybean nodule is not metabolically uniform, with several distinctive patterns of metabolite distributions through the nodule’s anatomy. Specifically, using two- and three-dimensional matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry imaging (MALDI-FTICR MSI) metabolites showed i) cortex localization, ii) a gradual increase toward the central infection zone, iii) uniform localization through whole nodule, iv) uniform localization throughout the infection zone, or v) asymmetric distribution within the infection zone. Some molecules belonging to the same or correlated metabolic pathways were found localized with different patterns, indicating localized differences in their synthesis/turnover. Special attention was given to asymmetrical distributed metabolites (e.g., S-adenosylmethionine; SAMe), where some small-scale patterns were detected in individual sections, and 3D reconstruction enabled resolution of whole nodule molecular distributions. These results hint at a complex, spatially differentiated metabolism occurring in soybean root nodules.},
doi = {10.1038/s41396-018-0188-8},
journal = {The ISME Journal},
number = 9,
volume = 12,
place = {United Kingdom},
year = {Wed Jun 13 00:00:00 EDT 2018},
month = {Wed Jun 13 00:00:00 EDT 2018}
}
https://doi.org/10.1038/s41396-018-0188-8
Web of Science
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