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Title: Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2

Abstract

Recent studies reported miR-497 exhibited inhibitory effects in various cancers. However, whether miR-497 is involved in inhibiting angiogenesis, which is critical for tumor growth and metastasis, is still unknown. The purpose of this study was to investigate the potential role of miR-497 in tumor angiogenesis. In this work, cell proliferation and apoptosis analyses were conducted to explore the potential function of miR-497 in HUVECs by using MTT and TUNEL assays. Western blotting (WB) was employed to validate the downstream targets of miR-497. Furthermore, in order to disclose the role of miR-497 on angiogenesis, VEGFR2-luc transgenic mice were treated with miR-497 mimic and applied to monitor tumor angiogenesis and growth by in vivo bioluminescent imaging (BLI). The results demonstrated that overexpression of miR-497 showed inhibitory effects on VEGFR2 activation and downstream Raf/MEK/ERK signal pathways in vitro and in vivo. Moreover, overexpression of miR-497 effectively induced HUVECs apoptosis by targeting VEGFR2 and downstream PI3K/AKT signaling pathway. Furthermore, miR-497 exhibited anti-angiogenesis and anti-tumor effects in the VEGFR2-luc breast tumor model proven by BLI, WB and immunohistochemistry analysis. In summary, miR-497 inhibits tumor angiogenesis and growth via targeting VEGFR2, indicating miR-497 can be explored as a potential drug candidate for cancer therapy.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [3];  [3];  [5];  [3]
  1. College of Heilongjiang Province, Harbin, Heilongjiang (China). Key Laboratory of Molecular Imaging; Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang (China). Department of Cardiology; Stanford Univ., CA (United States). Molecular Imaging Program at Stanford, Department of Radiology and Bio-X Program
  2. Third Hospital of Harbin Medical University, Harbin, Heilongjiang (China). Department of Anesthesiology
  3. College of Heilongjiang Province, Harbin, Heilongjiang (China). Key Laboratory of Molecular Imaging; Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang (China). Department of Radiology
  4. Second Hospital of Harbin Medical University, Harbin, Heilongjiang (China). Department of Cardiology
  5. Stanford Univ., CA (United States). Molecular Imaging Program at Stanford, Department of Radiology and Bio-X Program
  6. Harbin Medical University, Daqing, Heilongjiang (China). College of Pharmacy
Publication Date:
Research Org.:
Stanford Univ., CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1440561
Grant/Contract Number:  
SC0008397
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 5; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats

Tu, Yingfeng, Liu, Li, Zhao, Dongliang, Liu, Youbin, Ma, Xiaowei, Fan, Yuhua, Wan, Lin, Huang, Tao, Cheng, Zhen, and Shen, Baozhong. Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2. United States: N. p., 2015. Web. doi:10.1038/srep13827.
Tu, Yingfeng, Liu, Li, Zhao, Dongliang, Liu, Youbin, Ma, Xiaowei, Fan, Yuhua, Wan, Lin, Huang, Tao, Cheng, Zhen, & Shen, Baozhong. Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2. United States. doi:10.1038/srep13827.
Tu, Yingfeng, Liu, Li, Zhao, Dongliang, Liu, Youbin, Ma, Xiaowei, Fan, Yuhua, Wan, Lin, Huang, Tao, Cheng, Zhen, and Shen, Baozhong. Tue . "Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2". United States. doi:10.1038/srep13827. https://www.osti.gov/servlets/purl/1440561.
@article{osti_1440561,
title = {Overexpression of miRNA-497 inhibits tumor angiogenesis by targeting VEGFR2},
author = {Tu, Yingfeng and Liu, Li and Zhao, Dongliang and Liu, Youbin and Ma, Xiaowei and Fan, Yuhua and Wan, Lin and Huang, Tao and Cheng, Zhen and Shen, Baozhong},
abstractNote = {Recent studies reported miR-497 exhibited inhibitory effects in various cancers. However, whether miR-497 is involved in inhibiting angiogenesis, which is critical for tumor growth and metastasis, is still unknown. The purpose of this study was to investigate the potential role of miR-497 in tumor angiogenesis. In this work, cell proliferation and apoptosis analyses were conducted to explore the potential function of miR-497 in HUVECs by using MTT and TUNEL assays. Western blotting (WB) was employed to validate the downstream targets of miR-497. Furthermore, in order to disclose the role of miR-497 on angiogenesis, VEGFR2-luc transgenic mice were treated with miR-497 mimic and applied to monitor tumor angiogenesis and growth by in vivo bioluminescent imaging (BLI). The results demonstrated that overexpression of miR-497 showed inhibitory effects on VEGFR2 activation and downstream Raf/MEK/ERK signal pathways in vitro and in vivo. Moreover, overexpression of miR-497 effectively induced HUVECs apoptosis by targeting VEGFR2 and downstream PI3K/AKT signaling pathway. Furthermore, miR-497 exhibited anti-angiogenesis and anti-tumor effects in the VEGFR2-luc breast tumor model proven by BLI, WB and immunohistochemistry analysis. In summary, miR-497 inhibits tumor angiogenesis and growth via targeting VEGFR2, indicating miR-497 can be explored as a potential drug candidate for cancer therapy.},
doi = {10.1038/srep13827},
journal = {Scientific Reports},
number = 1,
volume = 5,
place = {United States},
year = {2015},
month = {9}
}

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