Building a genome engineering toolbox in nonmodel prokaryotic microbes
Abstract
The realization of a sustainable bioeconomy requires our ability to understand and engineer complex design principles for the development of platform organisms capable of efficient conversion of cheap and sustainable feedstocks (e.g. sunlight, CO2, non-food biomass) to biofuels and bioproducts at sufficient titers and costs. For model microbes such as E. coli, advances in DNA reading and writing technologies are driving adoption of new paradigms for engineering biological systems. Unfortunately, microbes with properties of interest for the utilization of cheap and renewable feedstocks such as photosynthesis, autotrophic growth, and cellulose degradation have very few, if any, genetic tools for metabolic engineering. Therefore, it is important to begin to develop “design rules” for building a genetic toolbox for novel microbes. Here, we present an overview of our current understanding of these rules for the genetic manipulation of prokaryotic microbes and available genetic tools to expand our ability to genetically engineer non-model systems.
- Authors:
-
[1];
[1]
- National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
- Univ. of Colorado, Boulder, CO (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1439175
- Alternate Identifier(s):
- OSTI ID: 1439401; OSTI ID: 1558846
- Report Number(s):
- NREL/JA-2700-71612
Journal ID: ISSN 0006-3592
- Grant/Contract Number:
- AC36-08GO28308; SC0018368
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology and Bioengineering
- Additional Journal Information:
- Journal Volume: 115; Journal Issue: 9; Journal ID: ISSN 0006-3592
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; bioengineering; genetic tools; genome editing; genome engineering; CRISPR; transformation
Citation Formats
Freed, Emily, Fenster, Jacob, Smolinski, Sharon L., Walker, Julie, Henard, Calvin A., Gill, Ryan, and Eckert, Carrie A. Building a genome engineering toolbox in nonmodel prokaryotic microbes. United States: N. p., 2018.
Web. doi:10.1002/bit.26727.
Freed, Emily, Fenster, Jacob, Smolinski, Sharon L., Walker, Julie, Henard, Calvin A., Gill, Ryan, & Eckert, Carrie A. Building a genome engineering toolbox in nonmodel prokaryotic microbes. United States. https://doi.org/10.1002/bit.26727
Freed, Emily, Fenster, Jacob, Smolinski, Sharon L., Walker, Julie, Henard, Calvin A., Gill, Ryan, and Eckert, Carrie A. Fri .
"Building a genome engineering toolbox in nonmodel prokaryotic microbes". United States. https://doi.org/10.1002/bit.26727. https://www.osti.gov/servlets/purl/1439175.
@article{osti_1439175,
title = {Building a genome engineering toolbox in nonmodel prokaryotic microbes},
author = {Freed, Emily and Fenster, Jacob and Smolinski, Sharon L. and Walker, Julie and Henard, Calvin A. and Gill, Ryan and Eckert, Carrie A.},
abstractNote = {The realization of a sustainable bioeconomy requires our ability to understand and engineer complex design principles for the development of platform organisms capable of efficient conversion of cheap and sustainable feedstocks (e.g. sunlight, CO2, non-food biomass) to biofuels and bioproducts at sufficient titers and costs. For model microbes such as E. coli, advances in DNA reading and writing technologies are driving adoption of new paradigms for engineering biological systems. Unfortunately, microbes with properties of interest for the utilization of cheap and renewable feedstocks such as photosynthesis, autotrophic growth, and cellulose degradation have very few, if any, genetic tools for metabolic engineering. Therefore, it is important to begin to develop “design rules” for building a genetic toolbox for novel microbes. Here, we present an overview of our current understanding of these rules for the genetic manipulation of prokaryotic microbes and available genetic tools to expand our ability to genetically engineer non-model systems.},
doi = {10.1002/bit.26727},
journal = {Biotechnology and Bioengineering},
number = 9,
volume = 115,
place = {United States},
year = {Fri May 11 00:00:00 EDT 2018},
month = {Fri May 11 00:00:00 EDT 2018}
}
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