The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin

Alazi, Ebru; Niu, Jing; Kowalczyk, Joanna E.; Peng, Mao; Aguilar Pontes, Maria Victoria; van Kan, Jan A.  L.; Visser, Jaap; de Vries, Ronald P.; Ram, Arthur F.  J.

doi:10.1002/1873-3468.12211

Title: The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin

Journal Article · Fri May 13 04:00:00 UTC 2016 · FEBS Letters

DOI: https://doi.org/10.1002/1873-3468.12211 · OSTI ID:1437993

Alazi, Ebru ^[1]; Niu, Jing ^[2]; Kowalczyk, Joanna E. ^[3]; Peng, Mao ^[3]; Aguilar Pontes, Maria Victoria ^[3]; van Kan, Jan A. L. ^[4]; Visser, Jaap ^[5]; de Vries, Ronald P. ^[3]; Ram, Arthur F. J. ^[2]

Leiden Univ. (Netherlands); Leiden University
Leiden Univ. (Netherlands)
Univ. of Utrecht (Netherlands)
Wageningen Univ. (Netherlands)
Leiden Univ. (Netherlands); Univ. of Utrecht (Netherlands)

We identified the d-galacturonic acid (GA)-responsive transcriptional activator GaaR of the saprotrophic fungus, Aspergillus niger, which was found to be essential for growth on GA and polygalacturonic acid (PGA). Growth of the ΔgaaR strain was reduced on complex pectins. Genome-wide expression analysis showed that GaaR is required for the expression of genes necessary to release GA from PGA and more complex pectins, to transport GA into the cell, and to induce the GA catabolic pathway. Residual growth of ΔgaaR on complex pectins is likely due to the expression of pectinases acting on rhamnogalacturonan and subsequent metabolism of the monosaccharides other than GA.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-93ER20097

OSTI ID:: 1437993

Journal Information:: FEBS Letters, Journal Name: FEBS Letters Journal Issue: 12 Vol. 590; ISSN 0014-5793

Publisher:: Federation of European Biochemical SocietiesCopyright Statement

Country of Publication:: United States

Language:: English

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Blachowicz, Adriana; Romsdahl, Jillian; Chiang, Abby J.; +6 more

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Title: The transcriptional activator GaaR of Aspergillus niger is required for release and utilization of d- galacturonic acid from pectin

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