GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase

Ishikawa, Toshiki; Fang, Lin; Rennie, Emilie A.; Sechet, Julien; Yan, Jingwei; Jing, Beibei; Moore, William; Cahoon, Edgar B.; Scheller, Henrik V.; Kawai-Yamada, Maki; Mortimer, Jenny C.

doi:10.1104/pp.18.00396

Title: GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase

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Abstract

Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structure, are the major sphingolipids in the plant plasma membrane. Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis thaliana, and demonstrated that the glycan headgroup is essential for plant viability. However, the function of GIPCs and the significance of their structural variation are poorly understood. Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain N-acetylglucosamine (GlcNAc) and glucosamine (GlcN; collectively GlcN(Ac)). In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants. However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal. Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup. Lastly, this study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function. We also identify amore »« less

Authors:

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Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan
Joint BioEnergy Institute, Emeryville, California 94608, Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley, California 94720
Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley, California 94720, Department of Plant and Molecular Biology, University of California, Berkeley, California 94720, Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588
Joint BioEnergy Institute, Emeryville, California 94608, Biosciences Area, Lawrence Berkeley National Laboratory, Berkeley, California 94720, Department of Plant and Molecular Biology, University of California, Berkeley, California 94720
Center for Plant Science Innovation and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588

Publication Date:: Mon May 14 00:00:00 EDT 2018

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)

OSTI Identifier:: 1437090

Alternate Identifier(s):: OSTI ID: 1457008

Grant/Contract Number:: AC02-05CH11231; 17K15411; 26292190; 1158500

Resource Type:: Published Article

Journal Name:: Plant Physiology (Bethesda)

Additional Journal Information:: Journal Name: Plant Physiology (Bethesda) Journal Volume: 177 Journal Issue: 3; Journal ID: ISSN 0032-0889

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Ishikawa, Toshiki, Fang, Lin, Rennie, Emilie A., Sechet, Julien, Yan, Jingwei, Jing, Beibei, Moore, William, Cahoon, Edgar B., Scheller, Henrik V., Kawai-Yamada, Maki, and Mortimer, Jenny C. GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.  United States: N. p., 2018. 
Web.  doi:10.1104/pp.18.00396.

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                    Ishikawa, Toshiki, Fang, Lin, Rennie, Emilie A., Sechet, Julien, Yan, Jingwei, Jing, Beibei, Moore, William, Cahoon, Edgar B., Scheller, Henrik V., Kawai-Yamada, Maki, & Mortimer, Jenny C. GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.  United States.  https://doi.org/10.1104/pp.18.00396

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                    Ishikawa, Toshiki, Fang, Lin, Rennie, Emilie A., Sechet, Julien, Yan, Jingwei, Jing, Beibei, Moore, William, Cahoon, Edgar B., Scheller, Henrik V., Kawai-Yamada, Maki, and Mortimer, Jenny C. Mon .  
"GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase".  United States.  https://doi.org/10.1104/pp.18.00396.

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@article{osti_1437090,

  title        = {GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase},

  author       = {Ishikawa, Toshiki and Fang, Lin and Rennie, Emilie A. and Sechet, Julien and Yan, Jingwei and Jing, Beibei and Moore, William and Cahoon, Edgar B. and Scheller, Henrik V. and Kawai-Yamada, Maki and Mortimer, Jenny C.},

  abstractNote = {Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structure, are the major sphingolipids in the plant plasma membrane. Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis thaliana, and demonstrated that the glycan headgroup is essential for plant viability. However, the function of GIPCs and the significance of their structural variation are poorly understood. Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain N-acetylglucosamine (GlcNAc) and glucosamine (GlcN; collectively GlcN(Ac)). In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants. However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal. Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup. Lastly, this study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function. We also identify a possible role for GIPCs in maintaining cell-cell adhesion.},

  doi          = {10.1104/pp.18.00396},

  journal      = {Plant Physiology (Bethesda)},

  number       = 3,

  volume       = 177,

  place        = {United States},

  year         = {Mon May 14 00:00:00 EDT 2018},

  month        = {Mon May 14 00:00:00 EDT 2018}

}

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