Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component

Hernandez, Carina; Birktoft, Jens J.; Ohayon, Yoel P.; Chandrasekaran, Arun Richard; Abdallah, Hatem; Sha, Ruojie; Stojanoff, Vivian; Mao, Chengde; Seeman, Nadrian C.

doi:10.1016/j.chembiol.2017.08.018

Title: Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component

Abstract

There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. Furthermore, the work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding.

Authors:: Hernandez, Carina; Birktoft, Jens J.; Ohayon, Yoel P.; Chandrasekaran, Arun Richard; Abdallah, Hatem; Sha, Ruojie; Stojanoff, Vivian; Mao, Chengde; Seeman, Nadrian C.

Publication Date:: Wed Nov 01 00:00:00 EDT 2017

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1482398

Alternate Identifier(s):: OSTI ID: 1435732

Report Number(s):: BNL-203483-2018-JAAM
Journal ID: ISSN 2451-9456; S2451945617303161; PII: S2451945617303161

Grant/Contract Number:: SC0007991; SC0012704

Resource Type:: Published Article

Journal Name:: Cell Chemical Biology

Additional Journal Information:: Journal Name: Cell Chemical Biology Journal Volume: 24 Journal Issue: 11; Journal ID: ISSN 2451-9456

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 36 MATERIALS SCIENCE; torsionally stressed DNA; structural DNA nanotechnology; self-assembled 3D; DNA crystals; X-ray diffraction; designed crystals

Citation Formats


                    Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, and Seeman, Nadrian C. Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component.  United States: N. p., 2017. 
Web.  doi:10.1016/j.chembiol.2017.08.018.

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                    Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, & Seeman, Nadrian C. Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component.  United States.  https://doi.org/10.1016/j.chembiol.2017.08.018

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                    Hernandez, Carina, Birktoft, Jens J., Ohayon, Yoel P., Chandrasekaran, Arun Richard, Abdallah, Hatem, Sha, Ruojie, Stojanoff, Vivian, Mao, Chengde, and Seeman, Nadrian C. Wed .  
"Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component".  United States.  https://doi.org/10.1016/j.chembiol.2017.08.018.

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@article{osti_1482398,

  title        = {Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component},

  author       = {Hernandez, Carina and Birktoft, Jens J. and Ohayon, Yoel P. and Chandrasekaran, Arun Richard and Abdallah, Hatem and Sha, Ruojie and Stojanoff, Vivian and Mao, Chengde and Seeman, Nadrian C.},

  abstractNote = {There is an increasing appreciation for structural diversity of DNA that is of interest to both DNA nanotechnology and basic biology. Here, we have explored how DNA responds to torsional stress by building on a previously reported two-turn DNA tensegrity triangle and demonstrating that we could introduce an extra nucleotide pair (np) into the original sequence without affecting assembly and crystallization. The extra np imposes a significant torsional stress, which is accommodated by global changes throughout the B-DNA duplex and the DNA lattice. Furthermore, the work reveals a near-atomic structure of naked DNA under a torsional stress of approximately 14%, and thus provides an example of DNA distortions that occur without a requirement for either an external energy source or the free energy available from protein or drug binding.},

  doi          = {10.1016/j.chembiol.2017.08.018},

  journal      = {Cell Chemical Biology},

  number       = 11,

  volume       = 24,

  place        = {United States},

  year         = {Wed Nov 01 00:00:00 EDT 2017},

  month        = {Wed Nov 01 00:00:00 EDT 2017}

}

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Works referencing / citing this record:

A study on a special DNA nanotube assembled from two single-stranded tiles
journal, January 2019

Xu, Fei; Wu, Tingfang; Shi, Xiaolong
Nanotechnology, Vol. 30, Issue 11
DOI: 10.1088/1361-6528/aaf9bc

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Title: Self-Assembly of 3D DNA Crystals Containing a Torsionally Stressed Component

Abstract

Citation Formats

A study on a special DNA nanotube assembled from two single-stranded tiles journal, January 2019

A study on a special DNA nanotube assembled from two single-stranded tiles
journal, January 2019