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Title: Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity

Abstract

The conversion of biomass-derived sugars and aromatic molecules to cis,cis-muconic acid (referred to hereafter as muconic acid or muconate) has been of recent interest owing to its facile conversion to adipic acid, an important commodity chemical. Metabolic routes to produce muconate from both sugars and many lignin-derived aromatic compounds require the use of a decarboxylase to convert protocatechuate (PCA, 3,4-dihydroxybenzoate) to catechol (1,2-dihydroxybenzene), two central aromatic intermediates in this pathway. Several studies have identified the PCA decarboxylase as a metabolic bottleneck, causing an accumulation of PCA that subsequently reduces muconate production. A recent study showed that activity of the PCA decarboxylase is enhanced by co-expression of two genetically associated proteins, one of which likely produces a flavin-derived cofactor utilized by the decarboxylase. Using entirely genome-integrated gene expression, we have engineered Pseudomonas putida KT2440-derived strains to produce muconate from either aromatic molecules or sugars and demonstrate in both cases that co-expression of these decarboxylase associated proteins reduces PCA accumulation and enhances muconate production relative to strains expressing the PCA decarboxylase alone. In bioreactor experiments, co-expression increased the specific productivity (mg/g cells/h) of muconate from the aromatic lignin monomer p-coumarate by 50% and resulted in a titer of >15 g/L. In strainsmore » engineered to produce muconate from glucose, co-expression more than tripled the titer, yield, productivity, and specific productivity, with the best strain producing 4.92+/-0.48 g/L muconate. Furthermore, this study demonstrates that overcoming the PCA decarboxylase bottleneck can increase muconate yields from biomass-derived sugars and aromatic molecules in industrially relevant strains and cultivation conditions.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1433729
Alternate Identifier(s):
OSTI ID: 1254108
Report Number(s):
NREL/JA-5100-66472
Journal ID: ISSN 2214-0301; S2214030116300128; PII: S2214030116300128
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Metabolic Engineering Communications
Additional Journal Information:
Journal Name: Metabolic Engineering Communications Journal Volume: 3 Journal Issue: C; Journal ID: ISSN 2214-0301
Publisher:
Elsevier
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; protocatechuate decarboxylase; Pseudomonas Putida KT2440; cis,cis-muconate; muconic acid; lignin valorization

Citation Formats

Johnson, Christopher W., Salvachúa, Davinia, Khanna, Payal, Smith, Holly, Peterson, Darren J., and Beckham, Gregg T. Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity. Netherlands: N. p., 2016. Web. doi:10.1016/j.meteno.2016.04.002.
Johnson, Christopher W., Salvachúa, Davinia, Khanna, Payal, Smith, Holly, Peterson, Darren J., & Beckham, Gregg T. Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity. Netherlands. doi:10.1016/j.meteno.2016.04.002.
Johnson, Christopher W., Salvachúa, Davinia, Khanna, Payal, Smith, Holly, Peterson, Darren J., and Beckham, Gregg T. Thu . "Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity". Netherlands. doi:10.1016/j.meteno.2016.04.002.
@article{osti_1433729,
title = {Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity},
author = {Johnson, Christopher W. and Salvachúa, Davinia and Khanna, Payal and Smith, Holly and Peterson, Darren J. and Beckham, Gregg T.},
abstractNote = {The conversion of biomass-derived sugars and aromatic molecules to cis,cis-muconic acid (referred to hereafter as muconic acid or muconate) has been of recent interest owing to its facile conversion to adipic acid, an important commodity chemical. Metabolic routes to produce muconate from both sugars and many lignin-derived aromatic compounds require the use of a decarboxylase to convert protocatechuate (PCA, 3,4-dihydroxybenzoate) to catechol (1,2-dihydroxybenzene), two central aromatic intermediates in this pathway. Several studies have identified the PCA decarboxylase as a metabolic bottleneck, causing an accumulation of PCA that subsequently reduces muconate production. A recent study showed that activity of the PCA decarboxylase is enhanced by co-expression of two genetically associated proteins, one of which likely produces a flavin-derived cofactor utilized by the decarboxylase. Using entirely genome-integrated gene expression, we have engineered Pseudomonas putida KT2440-derived strains to produce muconate from either aromatic molecules or sugars and demonstrate in both cases that co-expression of these decarboxylase associated proteins reduces PCA accumulation and enhances muconate production relative to strains expressing the PCA decarboxylase alone. In bioreactor experiments, co-expression increased the specific productivity (mg/g cells/h) of muconate from the aromatic lignin monomer p-coumarate by 50% and resulted in a titer of >15 g/L. In strains engineered to produce muconate from glucose, co-expression more than tripled the titer, yield, productivity, and specific productivity, with the best strain producing 4.92+/-0.48 g/L muconate. Furthermore, this study demonstrates that overcoming the PCA decarboxylase bottleneck can increase muconate yields from biomass-derived sugars and aromatic molecules in industrially relevant strains and cultivation conditions.},
doi = {10.1016/j.meteno.2016.04.002},
journal = {Metabolic Engineering Communications},
number = C,
volume = 3,
place = {Netherlands},
year = {2016},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1016/j.meteno.2016.04.002

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Works referencing / citing this record:

Microbial Genes for a Circular and Sustainable Bio-PET Economy
journal, May 2019

  • Salvador, Manuel; Abdulmutalib, Umar; Gonzalez, Jaime
  • Genes, Vol. 10, Issue 5
  • DOI: 10.3390/genes10050373

Microbial Genes for a Circular and Sustainable Bio-PET Economy
journal, May 2019

  • Salvador, Manuel; Abdulmutalib, Umar; Gonzalez, Jaime
  • Genes, Vol. 10, Issue 5
  • DOI: 10.3390/genes10050373