Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002
Abstract
RNase III is a ribonuclease that recognizes and cleaves double-stranded RNA. Across bacteria, RNase III is involved in rRNA maturation, CRISPR RNA maturation, controlling gene expression, and turnover of messenger RNAs. Many organisms have only one RNase III while others have both a full-length RNase III and another version that lacks a double-stranded RNA binding domain (mini-III). The genome of the cyanobacterium Synechococcus sp. strain PCC 7002 (PCC 7002) encodes three homologs of RNase III, two full-length and one mini-III, that are not essential even when deleted in combination. To discern if each enzyme had distinct responsibilities, we collected and sequenced global RNA samples from the wild type strain, the single, double, and triple RNase III mutants. Approximately 20% of genes were differentially expressed in various mutants with some operons and regulons showing complex changes in expression levels between mutants. Two RNase III's had a role in 23S rRNA maturation and the third was involved in copy number regulation one of six native plasmids. In vitro, purified RNase III enzymes were capable of cleaving some of the known Escherichia coli RNase III target sequences, highlighting the remarkably conserved substrate specificity between organisms yet complex regulation of gene expression.
- Authors:
-
[1]
- Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA, Microbiology Doctoral Training Program, University of Wisconsin-Madison, Madison, WI 53706, USA
- Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI 53706, USA
- Publication Date:
- Research Org.:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1430778
- Alternate Identifier(s):
- OSTI ID: 1502458
- Grant/Contract Number:
- SC0010329
- Resource Type:
- Published Article
- Journal Name:
- Nucleic Acids Research
- Additional Journal Information:
- Journal Name: Nucleic Acids Research Journal Volume: 46 Journal Issue: 4; Journal ID: ISSN 0305-1048
- Publisher:
- Oxford University Press
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Gordon, Gina C., Cameron, Jeffrey C., and Pfleger, Brian F.. Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002. United Kingdom: N. p., 2018.
Web. doi:10.1093/nar/gky041.
Gordon, Gina C., Cameron, Jeffrey C., & Pfleger, Brian F.. Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002. United Kingdom. https://doi.org/10.1093/nar/gky041
Gordon, Gina C., Cameron, Jeffrey C., and Pfleger, Brian F.. Wed .
"Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002". United Kingdom. https://doi.org/10.1093/nar/gky041.
@article{osti_1430778,
title = {Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002},
author = {Gordon, Gina C. and Cameron, Jeffrey C. and Pfleger, Brian F.},
abstractNote = {RNase III is a ribonuclease that recognizes and cleaves double-stranded RNA. Across bacteria, RNase III is involved in rRNA maturation, CRISPR RNA maturation, controlling gene expression, and turnover of messenger RNAs. Many organisms have only one RNase III while others have both a full-length RNase III and another version that lacks a double-stranded RNA binding domain (mini-III). The genome of the cyanobacterium Synechococcus sp. strain PCC 7002 (PCC 7002) encodes three homologs of RNase III, two full-length and one mini-III, that are not essential even when deleted in combination. To discern if each enzyme had distinct responsibilities, we collected and sequenced global RNA samples from the wild type strain, the single, double, and triple RNase III mutants. Approximately 20% of genes were differentially expressed in various mutants with some operons and regulons showing complex changes in expression levels between mutants. Two RNase III's had a role in 23S rRNA maturation and the third was involved in copy number regulation one of six native plasmids. In vitro, purified RNase III enzymes were capable of cleaving some of the known Escherichia coli RNase III target sequences, highlighting the remarkably conserved substrate specificity between organisms yet complex regulation of gene expression.},
doi = {10.1093/nar/gky041},
journal = {Nucleic Acids Research},
number = 4,
volume = 46,
place = {United Kingdom},
year = {2018},
month = {1}
}
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Figures / Tables:
Figure 1: Role of RNase III in rRNA processing. (A) BioAnalyzer traces of RNA extracted from WT and select RNase III mutants. (B) Extensions of 23S rRNA at the 5′ and 3′ ends in A0061 A2542 A0384. A diagram of the rRNA operon shows primers used to amplify cDNA mademore »
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