Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002

Gordon, Gina C.; Cameron, Jeffrey C.; Pfleger, Brian F.

doi:10.1093/nar/gky041

Title: Distinct and redundant functions of three homologs of RNase III in the cyanobacterium Synechococcus sp. strain PCC 7002

Journal Article · 24 January 2018 · Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gky041 · OSTI ID:1430778

Gordon, Gina C. ^[1]; Cameron, Jeffrey C. ^[2];

^[1]

Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering, and Microbiology Doctoral Training Program
Univ. of Wisconsin, Madison, WI (United States). Dept. of Chemical and Biological Engineering; Univ. of Colorado, Boulder, CO (United States). Dept. of Chemistry and Biochemistry

RNase III is a ribonuclease that recognizes and cleaves double-stranded RNA. Across bacteria, RNase III is involved in rRNA maturation, CRISPR RNA maturation, controlling gene expression, and turnover of messenger RNAs. Many organisms have only one RNase III while others have both a full-length RNase III and another version that lacks a double-stranded RNA binding domain (mini-III). The genome of the cyanobacterium Synechococcus sp. strain PCC 7002 (PCC 7002) encodes three homologs of RNase III, two full-length and one mini-III, that are not essential even when deleted in combination. To discern if each enzyme had distinct responsibilities, we collected and sequenced global RNA samples from the wild type strain, the single, double, and triple RNase III mutants. Approximately 20% of genes were differentially expressed in various mutants with some operons and regulons showing complex changes in expression levels between mutants. Two RNase III's had a role in 23S rRNA maturation and the third was involved in copy number regulation one of six native plasmids. In vitro, purified RNase III enzymes were capable of cleaving some of the known Escherichia coli RNase III target sequences, highlighting the remarkably conserved substrate specificity between organisms yet complex regulation of gene expression.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC); USDOE

Grant/Contract Number:: SC0010329

OSTI ID:: 1430778

Alternate ID(s):: OSTI ID: 1502458

Journal Information:: Nucleic Acids Research, Vol. 46, Issue 4; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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