Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated
Abstract
Abstract Cellulose synthases (CESAs) are glycosyltransferases that catalyze formation of cellulose microfibrils in plant cell walls. Seed plant CESA isoforms cluster in six phylogenetic clades, whose non‐interchangeable members play distinct roles within cellulose synthesis complexes (CSCs). A ‘class specific region’ (CSR), with higher sequence similarity within versus between functional CESA classes, has been suggested to contribute to specific activities or interactions of different isoforms. We investigated CESA isoform specificity in the moss, Physcomitrella patens (Hedw.) B. S. G. to gain evolutionary insights into CESA structure/function relationships. Like seed plants, P. patens has oligomeric rosette‐type CSCs, but the PpCESAs diverged independently and form a separate CESA clade. We showed that P. patens has two functionally distinct CESAs classes, based on the ability to complement the gametophore‐negative phenotype of a ppcesa5 knockout line. Thus, non‐interchangeable CESA classes evolved separately in mosses and seed plants. However, testing of chimeric moss CESA genes for complementation demonstrated that functional class‐specificity is not determined by the CSR. Sequence analysis and computational modeling showed that the CSR is intrinsically disordered and contains predicted molecular recognition features, consistent with a possible role in CESA oligomerization and explaining the evolution of class‐specific sequences without selection for class‐specific function.
- Authors:
-
- Department of Biological Sciences University of Rhode Island 120 Flagg Road Kingston RI 02881 USA
- Department of Materials Science and Engineering North Carolina State University Raleigh NC 27695 USA
- Department of Crop and Soil Sciences and Department of Plant and Microbial Biology North Carolina State University Raleigh NC 27695 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1430743
- Grant/Contract Number:
- DE‐SC0001090
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Journal of Integrative Plant Biology
- Additional Journal Information:
- Journal Name: Journal of Integrative Plant Biology Journal Volume: 60 Journal Issue: 6; Journal ID: ISSN 1672-9072
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Scavuzzo‐Duggan, Tess R., Chaves, Arielle M., Singh, Abhishek, Sethaphong, Latsavongsakda, Slabaugh, Erin, Yingling, Yaroslava G., Haigler, Candace H., and Roberts, Alison W. Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated. United Kingdom: N. p., 2018.
Web. doi:10.1111/jipb.12637.
Scavuzzo‐Duggan, Tess R., Chaves, Arielle M., Singh, Abhishek, Sethaphong, Latsavongsakda, Slabaugh, Erin, Yingling, Yaroslava G., Haigler, Candace H., & Roberts, Alison W. Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated. United Kingdom. https://doi.org/10.1111/jipb.12637
Scavuzzo‐Duggan, Tess R., Chaves, Arielle M., Singh, Abhishek, Sethaphong, Latsavongsakda, Slabaugh, Erin, Yingling, Yaroslava G., Haigler, Candace H., and Roberts, Alison W. Fri .
"Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated". United Kingdom. https://doi.org/10.1111/jipb.12637.
@article{osti_1430743,
title = {Cellulose synthase ‘class specific regions’ are intrinsically disordered and functionally undifferentiated},
author = {Scavuzzo‐Duggan, Tess R. and Chaves, Arielle M. and Singh, Abhishek and Sethaphong, Latsavongsakda and Slabaugh, Erin and Yingling, Yaroslava G. and Haigler, Candace H. and Roberts, Alison W.},
abstractNote = {Abstract Cellulose synthases (CESAs) are glycosyltransferases that catalyze formation of cellulose microfibrils in plant cell walls. Seed plant CESA isoforms cluster in six phylogenetic clades, whose non‐interchangeable members play distinct roles within cellulose synthesis complexes (CSCs). A ‘class specific region’ (CSR), with higher sequence similarity within versus between functional CESA classes, has been suggested to contribute to specific activities or interactions of different isoforms. We investigated CESA isoform specificity in the moss, Physcomitrella patens (Hedw.) B. S. G. to gain evolutionary insights into CESA structure/function relationships. Like seed plants, P. patens has oligomeric rosette‐type CSCs, but the PpCESAs diverged independently and form a separate CESA clade. We showed that P. patens has two functionally distinct CESAs classes, based on the ability to complement the gametophore‐negative phenotype of a ppcesa5 knockout line. Thus, non‐interchangeable CESA classes evolved separately in mosses and seed plants. However, testing of chimeric moss CESA genes for complementation demonstrated that functional class‐specificity is not determined by the CSR. Sequence analysis and computational modeling showed that the CSR is intrinsically disordered and contains predicted molecular recognition features, consistent with a possible role in CESA oligomerization and explaining the evolution of class‐specific sequences without selection for class‐specific function.},
doi = {10.1111/jipb.12637},
journal = {Journal of Integrative Plant Biology},
number = 6,
volume = 60,
place = {United Kingdom},
year = {Fri Mar 30 00:00:00 EDT 2018},
month = {Fri Mar 30 00:00:00 EDT 2018}
}
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