Taxonomic patterns in the nitrogen assimilation of soil prokaryotes
Abstract
Summary Nitrogen (N) is frequently a limiting nutrient in soil; its availability can govern ecosystem functions such as primary production and decomposition. Assimilation of N by microorganisms impacts the availability of N in soil. Despite its established ecological significance, the contributions of microbial taxa to N assimilation are unknown. Here we measure N uptake and use by microbial phylotypes and taxonomic groups within a diverse assemblage of soil microbes through quantitative stable isotope probing (qSIP) with 15 N. Following incubation with 15 , distinct patterns of 15 N assimilation among taxonomic groups were observed. For instance, glucose addition stimulated 15 N assimilation in most members of Actinobacteria and Proteobacteria but generally decreased 15 N use by Firmicutes and Bacteriodetes. While is considered a preferred and universal source of N to prokaryotes, the majority (> 80%) of N assimilation in our soils could be attributed to a handful of active orders. Characterizing N assimilation of taxonomic groups with 15 N qSIP may provide a basis for understanding how microbial community composition influences N availability in the environment.
- Authors:
-
- Center for Ecosystem Science and Society Northern Arizona University Flagstaff AZ USA, Division of Plant and Soil Sciences West Virginia University Morgantown WV USA
- Center for Ecosystem Science and Society Northern Arizona University Flagstaff AZ USA
- Center for Ecosystem Science and Society Northern Arizona University Flagstaff AZ USA, Department of Biological Sciences Northern Arizona University Flagstaff AZ USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1426319
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Name: Environmental Microbiology Journal Volume: 20 Journal Issue: 3; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Morrissey, Ember M., Mau, Rebecca L., Schwartz, Egbert, Koch, Benjamin J., Hayer, Michaela, and Hungate, Bruce A. Taxonomic patterns in the nitrogen assimilation of soil prokaryotes. United Kingdom: N. p., 2018.
Web. doi:10.1111/1462-2920.14051.
Morrissey, Ember M., Mau, Rebecca L., Schwartz, Egbert, Koch, Benjamin J., Hayer, Michaela, & Hungate, Bruce A. Taxonomic patterns in the nitrogen assimilation of soil prokaryotes. United Kingdom. https://doi.org/10.1111/1462-2920.14051
Morrissey, Ember M., Mau, Rebecca L., Schwartz, Egbert, Koch, Benjamin J., Hayer, Michaela, and Hungate, Bruce A. Wed .
"Taxonomic patterns in the nitrogen assimilation of soil prokaryotes". United Kingdom. https://doi.org/10.1111/1462-2920.14051.
@article{osti_1426319,
title = {Taxonomic patterns in the nitrogen assimilation of soil prokaryotes},
author = {Morrissey, Ember M. and Mau, Rebecca L. and Schwartz, Egbert and Koch, Benjamin J. and Hayer, Michaela and Hungate, Bruce A.},
abstractNote = {Summary Nitrogen (N) is frequently a limiting nutrient in soil; its availability can govern ecosystem functions such as primary production and decomposition. Assimilation of N by microorganisms impacts the availability of N in soil. Despite its established ecological significance, the contributions of microbial taxa to N assimilation are unknown. Here we measure N uptake and use by microbial phylotypes and taxonomic groups within a diverse assemblage of soil microbes through quantitative stable isotope probing (qSIP) with 15 N. Following incubation with 15 , distinct patterns of 15 N assimilation among taxonomic groups were observed. For instance, glucose addition stimulated 15 N assimilation in most members of Actinobacteria and Proteobacteria but generally decreased 15 N use by Firmicutes and Bacteriodetes. While is considered a preferred and universal source of N to prokaryotes, the majority (> 80%) of N assimilation in our soils could be attributed to a handful of active orders. Characterizing N assimilation of taxonomic groups with 15 N qSIP may provide a basis for understanding how microbial community composition influences N availability in the environment.},
doi = {10.1111/1462-2920.14051},
journal = {Environmental Microbiology},
number = 3,
volume = 20,
place = {United Kingdom},
year = {Wed Mar 14 00:00:00 EDT 2018},
month = {Wed Mar 14 00:00:00 EDT 2018}
}
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https://doi.org/10.1111/1462-2920.14051
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