Measuring fluorescence polarization with a dichrometer

Sutherland, John C.

doi:10.1016/j.ab.2017.04.001

Title: Measuring fluorescence polarization with a dichrometer

Abstract

In this article, a method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.

Authors:

^[1]

Augusta Univ., Augusta, GA (United States). Dept. of Chemistry and Physics; Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Biology; East Carolina Univ., Greenville, NC (United States). Dept. of Physics

Publication Date:: Thu Apr 06 00:00:00 EDT 2017

Research Org.:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Augusta Univ., Augusta, GA (United States)

OSTI Identifier:: 1424976

Report Number(s):: BNL-200007-2018-JAAM
Journal ID: ISSN 0003-2697

Grant/Contract Number:: SC0012704

Resource Type:: Accepted Manuscript

Journal Name:: Analytical Biochemistry

Additional Journal Information:: Journal Volume: 532; Journal Issue: C; Journal ID: ISSN 0003-2697

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Anisotropy; Circular dichroism; Dichrometer; Linear dichroism; Photoelastic modulator; Polarization ratio

Citation Formats


                    Sutherland, John C. Measuring fluorescence polarization with a dichrometer.  United States: N. p., 2017. 
Web.  doi:10.1016/j.ab.2017.04.001.

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                    Sutherland, John C. Measuring fluorescence polarization with a dichrometer.  United States.  https://doi.org/10.1016/j.ab.2017.04.001

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                    Sutherland, John C. Thu .  
"Measuring fluorescence polarization with a dichrometer".  United States.  https://doi.org/10.1016/j.ab.2017.04.001.  https://www.osti.gov/servlets/purl/1424976.

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@article{osti_1424976,

  title        = {Measuring fluorescence polarization with a dichrometer},

  author       = {Sutherland, John C.},

  abstractNote = {In this article, a method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.},

  doi          = {10.1016/j.ab.2017.04.001},

  journal      = {Analytical Biochemistry},

  number       = C,

  volume       = 532,

  place        = {United States},

  year         = {Thu Apr 06 00:00:00 EDT 2017},

  month        = {Thu Apr 06 00:00:00 EDT 2017}

}

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Works referenced in this record:

Recording polarization of fluorescence spectrometer. Unique application of piezoelectric birefringence modulation
journal, April 1974

Wampler, John E.; DeSa, Richard J.
Analytical Chemistry, Vol. 46, Issue 4
DOI: 10.1021/ac60340a004

Emission and polarization spectrometer for biophysical spectroscopy
journal, March 1976

Sutherland, John Clark; Cimino, George D.; Lowe, John T.
Review of Scientific Instruments, Vol. 47, Issue 3
DOI: 10.1063/1.1134624

High-Sensitivity Fluorescence Anisotropy Detection of Protein-Folding Events: Application to α-Lactalbumin
journal, April 2001

Canet, Denis; Doering, Klaus; Dobson, Christopher M.
Biophysical Journal, Vol. 80, Issue 4
DOI: 10.1016/S0006-3495(01)76169-3

Linear dichroism of DNA: Characterization of the orientation distribution function caused by hydrodynamic shear
journal, April 2017

Sutherland, John C.
Analytical Biochemistry, Vol. 523
DOI: 10.1016/j.ab.2017.01.016

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Similar Records

Title: Measuring fluorescence polarization with a dichrometer

Abstract

Citation Formats

Recording polarization of fluorescence spectrometer. Unique application of piezoelectric birefringence modulation journal, April 1974

Emission and polarization spectrometer for biophysical spectroscopy journal, March 1976

High-Sensitivity Fluorescence Anisotropy Detection of Protein-Folding Events: Application to α-Lactalbumin journal, April 2001

Linear dichroism of DNA: Characterization of the orientation distribution function caused by hydrodynamic shear journal, April 2017

A quantitative characterization of interaction between prion protein with nucleic acids journal, July 2018

Recording polarization of fluorescence spectrometer. Unique application of piezoelectric birefringence modulation
journal, April 1974

Emission and polarization spectrometer for biophysical spectroscopy
journal, March 1976

High-Sensitivity Fluorescence Anisotropy Detection of Protein-Folding Events: Application to α-Lactalbumin
journal, April 2001

Linear dichroism of DNA: Characterization of the orientation distribution function caused by hydrodynamic shear
journal, April 2017

A quantitative characterization of interaction between prion protein with nucleic acids
journal, July 2018