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Title: Measuring fluorescence polarization with a dichrometer

In this article, a method for obtaining fluorescence polarization data from an instrument designed to measure circular and linear dichroism is compared with a previously reported approach. The new method places a polarizer between the sample and a detector mounted perpendicular to the direction of the incident beam and results in determination of the fluorescence polarization ratio, whereas the previous method does not use a polarizer and yields the fluorescence anisotropy. A similar analysis with the detector located axially with the excitation beam demonstrates that there is no frequency modulated signal due to fluorescence polarization in the absence of a polarizer.
Authors:
ORCiD logo [1]
  1. Augusta Univ., Augusta, GA (United States). Dept. of Chemistry and Physics; Brookhaven National Lab. (BNL), Upton, NY (United States). Dept. of Biology; East Carolina Univ., Greenville, NC (United States). Dept. of Physics
Publication Date:
Report Number(s):
BNL-200007-2018-JAAM
Journal ID: ISSN 0003-2697
Grant/Contract Number:
SC0012704
Type:
Accepted Manuscript
Journal Name:
Analytical Biochemistry
Additional Journal Information:
Journal Volume: 532; Journal Issue: C; Journal ID: ISSN 0003-2697
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Augusta Univ., Augusta, GA (United States)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Anisotropy; Circular dichroism; Dichrometer; Linear dichroism; Photoelastic modulator; Polarization ratio
OSTI Identifier:
1424976