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Title: Single-virus genomics reveals hidden cosmopolitan and abundant viruses

Microbes drive ecosystems under constraints imposed by viruses. However, a lack of virus genome information hinders our ability to answer fundamental, biological questions concerning microbial communities. Here we apply single-virus genomics (SVGs) to assess whether portions of marine viral communities are missed by current techniques. The majority of the here-identified 44 viral single-amplified genomes (vSAGs) are more abundant in global ocean virome data sets than published metagenome-assembled viral genomes or isolates. This indicates that vSAGs likely best represent the dsDNA viral populations dominating the oceans. Species-specific recruitment patterns and virome simulation data suggest that vSAGs are highly microdiverse and that microdiversity hinders the metagenomic assembly, which could explain why their genomes have not been identified before. Altogether, SVGs enable the discovery of some of the likely most abundant and ecologically relevant marine viral species, such as vSAG 37-F6, which were overlooked by other methodologies.
Authors:
 [1] ;  [2] ;  [1] ;  [3] ;  [1] ;  [4] ;  [1] ;  [5] ;  [6] ;  [6] ;  [7] ;  [5] ;  [1]
  1. University of Alicante (Spain). Department of Physiology, Genetics, and Microbiology
  2. The Barcelona Institute for Science and Technology (BIST) (Spain). Centre for Genomic Regulation (CRG; Universitat Pompeu Fabra (UPF), Barcelona (Spain)
  3. The Ohio State Univ., Columbus, OH (United States). Department of Microbiology
  4. Bigelow Laboratory for Ocean Sciences, East Boothbay, ME (United States)
  5. Institut de Ciencies del Mar (ICM), Barcelona (Spain). Department of Marine Biology and Oceanography
  6. Universidad Miguel Hernandez, San Juan (Spain). Evolutionary Genomics Group, Departamento de Produccion Vegetal y Microbiologia
  7. The Ohio State Univ., Columbus, OH (United States). Department of Microbiology and Department of Civil, Environmental and Geodetic Engineering
Publication Date:
Grant/Contract Number:
SC0010580
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Univ. of Arizona, Tucson, AZ (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; Genomic analysis; Microbial ecology; Sequencing; Virology
OSTI Identifier:
1424950